Article
Plant Sciences
Ruibing Chen, Yuejuan Bu, Junze Ren, Kyle A. Pelot, Xiangyang Hu, Yong Diao, Wansheng Chen, Philipp Zerbe, Lei Zhang
Summary: The study identified diverse diterpenoid biosynthesis pathways in Artemisia annua, showcasing their important roles in plant organ development and stress resistance. Results demonstrate that metabolic engineering of diterpenoids can increase artemisinin yield and plant stress resilience simultaneously. In addition, bioactive gibberellins GA(4)/GA(1) contribute to the formation of glandular trichomes.
Article
Biotechnology & Applied Microbiology
Zhiying Guo, Kai Hao, Zongyou Lv, Luyao Yu, Qitao Bu, Junze Ren, Henan Zhang, Ruibing Chen, Lei Zhang
Summary: This study identified a phytohormone-responsive miRNA, miR160, that negatively regulates glandular trichome development and artemisinin biosynthesis in Artemisia annua. The miR160-ARF1 module was found to be a key circuit in artemisinin biosynthesis, and this finding may contribute to the development of metabolic engineering strategies for high and stable production of artemisinin.
PLANT BIOTECHNOLOGY JOURNAL
(2023)
Article
Plant Sciences
Wei Qin, Yongpeng Li, Bowen Peng, Hang Liu, Tiantian Chen, Xin Yan, Yaojie Zhang, Chen Wang, Xinghao Yao, Xueqing Fu, Ling Li, Kexuan Tang
Summary: In this study, a method using laser capture microdissection (LCM) was developed to isolate glandular secretory trichomes (GSTs) from leaf epidermis without fixation. The method efficiently captured GSTs and enriched for related metabolites. This approach enables the study of pure plant cell populations and has the potential to discover novel secondary metabolites.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Agricultural Engineering
Yanchao Yuan, Qingjia Wang, Boqiang Tong, Dan Liu, Hanzhu Liu, Qingkang Yan, Hua Xin
Summary: The distribution of glandular trichomes (GTs) on the shooting of Lonicera japonica was observed, showing that they were mainly distributed along the veins on the leaf surface and evenly distributed on the stem surface. A new type of GTs with disc-shaped base was identified. Two novel HD-ZIP genes, LjROC3 and LjHD-ZIP1, were found to be up-regulated during GT development. Additionally, LjROC3 was found to increase GT density while LjHD-ZIP1 showed no significant difference.
INDUSTRIAL CROPS AND PRODUCTS
(2023)
Article
Plant Sciences
Zhaojun Wang, Yanhua Li, Hongying Zhang, Xiaoxiao Yan, Hong Cui
Summary: The study indicates that glandular and non-glandular trichomes in tobacco play different roles in herbivore defense, with glandular trichomes primarily enhancing aphid resistance and non-glandular trichomes strengthening herbivore resistance by accumulating defensive proteins.
PLANT CELL REPORTS
(2022)
Article
Plant Sciences
Lihui Xie, Tingxiang Yan, Ling Li, Minghui Chen, Danial Hassani, Yongpeng Li, Wei Qin, Hang Liu, Tiantian Chen, Xueqing Fu, Qian Shen, Jocelyn K. C. Rose, Kexuan Tang
Summary: The study identified key transcription factors, AaMYB16 and AaMYB5, that interact with AaHD1 to regulate glandular secretory trichomes (GSTs) initiation in plants. AaMYB16 was found to be a positive regulator while AaMYB5 had an inhibitory effect on GST initiation. The competitive interaction between AaMYB16 and AaMYB5 modulates the binding activity of AaHD1 promoter, providing new insights into the regulatory network controlling GST formation.
Article
Biochemistry & Molecular Biology
Meiyu Sun, Yanan Zhang, Li Zhu, Ningning Liu, Hongtong Bai, Guofeng Sun, Jinzheng Zhang, Lei Shi
Summary: This study successfully assembled and annotated the genome of T. quinquecostatus, providing significant insights into monoterpenoid biosynthesis and glandular secretory trichome development. This work is important for understanding the mechanisms of thyme biosynthesis and developing molecular breeding tools to enhance the production of bioactive secondary metabolites.
PLANT COMMUNICATIONS
(2022)
Review
Plant Sciences
Cailun A. S. Tanney, Rachel Backer, Anja Geitmann, Donald L. L. Smith
Summary: Cannabis is legalized in many countries for recreational and medical use, leading to a growing international market demand. The key compounds, cannabinoids, are produced by glandular trichomes on female flowers, which are the true source of revenue for the multi-billion-dollar industry.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Plant Sciences
Wei Qin, Lihui Xie, Yongpeng Li, Hang Liu, Xueqing Fu, Tiantian Chen, Danial Hassani, Ling Li, Xiaofen Sun, Kexuan Tang
Summary: The AaMYB17 gene has been identified as a positive regulator of GST initiation in Artemisia annua leaves, leading to increased artemisinin content. Overexpression or RNA interference of AaMYB17 does not affect plant growth or trichome morphology.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Agricultural Engineering
Jelena Stanojkovic, Sladana Todorovic, Ilinka Pecinar, Steva Levic, Snezana Calic, Dusica Janosevic
Summary: Glandular trichomes are secretory organs with diverse characteristics, such as shape, size, location, and composition of secondary metabolites. Sucrose has an impact on the development, density, and chemical composition of leaf glandular trichomes of in vitro cultured Inula britannica, affecting growth and biomass accumulation. A lack of sugar does not affect growth, but a high sugar concentration inhibits biomass production in vitro. Differences in the chemical profiles of glandular trichomes grown in different sugar concentrations mainly come from phenolic compounds.
INDUSTRIAL CROPS AND PRODUCTS
(2021)
Article
Plant Sciences
Sahana Basu, Surbhi Kumari, Pritee Subhadarshini, Aniket Kumar Rishu, Shashi Shekhar, Gautam Kumar
Summary: Soil salinity leading to sodium toxicity is a global challenge for agricultural productivity, and applying plant growth-promoting rhizobacteria (PGPR) can enhance plant salinity tolerance. This study found that the halotolerant Bacillus sp. BSE01 can improve salt stress endurance in chickpea by modulating hormonal signaling and maintaining cellular homeostasis.
PHYSIOLOGIA PLANTARUM
(2023)
Article
Biochemistry & Molecular Biology
Zhongxuan Feng, Lei Sun, Mingming Dong, Shanshan Fan, Kexin Shi, Yixin Qu, Liyan Zhu, Jinfeng Shi, Wujun Wang, Yihan Liu, Xiaofeng Chen, Yiqun Weng, Xingwang Liu, Huazhong Ren
Summary: Glandular trichomes (GTs) are specialized structures formed by plant epidermal cells to resist external stresses. They store and secrete secondary metabolites with agricultural and medicinal values. This study identified and analyzed 13 MYC transcription factors in the cucumber genome, and found that CsMYC2 negatively regulates GT formation, while CsMYC4, CsMYC5, CsMYC6, CsMYC7, and CsMYC8 positively regulate GT formation. CsMYC2 and CsMYC7 co-regulate the balance of GT development in an antagonistic way.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Plant Sciences
Evelyn Amrehn, Otmar Spring
Summary: The study investigated the structural and secretory activities of sunflower trichome cells using various types of microscopy, revealing the functional units and subcellular compartmentation of the trichome.
Article
Plant Sciences
Boran Dong, Xingxing Wang, Rui Jiang, Shiyuan Fang, Jinxing Li, Qing Li, Zong You Lv, Wan Sheng Chen
Summary: In this study, AaCycTL was found to regulate trichome development and affect the content of artemisinin in Artemisia annua. Overexpression of AaCycTL in Arabidopsis and A. annua resulted in changes in wax coverage on the plant surface and decreased trichome density. The interaction between AaCycTL and AaTAR1 was identified, indicating a potential role of AaCycTL in regulating trichome density and artemisinin biosynthesis.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Plant Sciences
L. Nomani, A. Zehra, S. Choudhary, K. I. Wani, M. Naeem, M. H. Siddiqui, M. M. A. Khan, T. Aftab
Summary: The study assessed the impact of exogenous hydrogen sulfide in alleviating copper stress on Artemisia annua, showing that hydrogen sulfide can restore the physiological and biochemical characteristics of copper-stressed plants.
Article
Plant Sciences
Jyoti Singh Jadaun, Neelam S. Sangwan, Lokesh K. Narnoliya, Neha Singh, Shilpi Bansal, Bhawana Mishra, Rajender Singh Sangwan
PHYSIOLOGIA PLANTARUM
(2017)
Article
Plant Sciences
Jyoti Singh Jadaun, Neelam Singh Sangwan, Lokesh Kumar Narnoliya, Sandhya Tripathi, Rajender Singh Sangwan
Article
Plant Sciences
Shiwani Maurya, Muktesh Chandra, Ritesh K. Yadav, Lokesh K. Narnoliya, Rajender S. Sangwan, Shilpi Bansal, Pankajpreet Sandhu, Umesh Singh, Devender Kumar, Neelam Singh Sangwan
Article
Plant Sciences
Bhawana Mishra, Neelam Singh Sangwan
PLANT GROWTH REGULATION
(2019)
Article
Plant Sciences
Bhawana Mishra, Sukhmal Chand, Neelam Singh Sangwan
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2019)
Article
Multidisciplinary Sciences
Sandhya Tripathi, Yashdeep Srivastava, Rajender Singh Sangwan, Neelam Singh Sangwan
SCIENTIFIC REPORTS
(2020)
Article
Plant Sciences
Jyoti Singh Jadaun, Amit Kumar Kushwaha, Neelam S. Sangwan, Lokesh Kumar Narnoliya, Smrati Mishra, Rajender Singh Sangwan
PLANT CELL REPORTS
(2020)
Article
Agricultural Engineering
Bhawana Mishra, Subir K. Bose, Neelam S. Sangwan
INDUSTRIAL CROPS AND PRODUCTS
(2020)
Article
Biochemistry & Molecular Biology
Muktesh Chandra, Shiwani Kushwaha, Neelam S. Sangwan
MOLECULAR BIOLOGY REPORTS
(2020)
Article
Plant Sciences
Sandeep K. Singh, Guru R. Valicherla, Anil K. Bikkasani, Srikanth H. Cheruvu, Zakir Hossain, Isha Taneja, Hafsa Ahmad, Kanumuri S. R. Raju, Neelam S. Sangwan, Shio K. Singh, Anil K. Dwivedi, Mohammad Wahajuddin, Jiaur R. Gayen
Summary: The study aimed to develop a precise method for analyzing Withanone (WN) in rat plasma, and investigate its in-vitro ADME behavior. Results showed that WN exhibited stability and high permeability in simulated gastric and intestinal environments, with high plasma protein binding. CYP phenotyping indicated extensive metabolism by CYP 3A4 and 1A2, though it did not potently inhibit any CYP isoenzymes in rat and human liver microsomes. The findings suggest that WN could be a promising phytochemical for neurological disorders based on its pharmacokinetics and pharmacodynamics.
JOURNAL OF ETHNOPHARMACOLOGY
(2021)
Article
Plant Sciences
Lokesh Kumar Narnoliya, Neelam Sangwan, Jyoti Singh Jadaun, Shilpi Bansal, Rajender Singh Sangwan
Summary: This study cloned and characterized the recombinant caffeic acid 3-O-methyltransferase gene from Neem, demonstrating its crucial role in ferulic acid biosynthesis. The heterologous expression of the gene in different plant species provided insights into its biological function in plants, highlighting potential applications in metabolic engineering for the biosynthesis of therapeutically important phytomolecules.
Article
Oncology
Deepti Diwan, Lei Cheng, Zeba Usmani, Minaxi Sharma, Nicola Holden, Nicholas Willoughby, Neelam Sangwan, Rama Raju Baadhe, Chenchen Liu, Vijai Kumar Gupta
Summary: The success of conventional cancer therapeutics is limited by antibiotic resistance and lack of specificity in anti-tumor drugs. Microbial-derived bioactive molecules are offering a new strategic solution by selectively targeting cancer cells. This review provides a comprehensive summary of the anti-carcinogenic properties of these molecules and discusses effective therapeutic applications to minimize systemic side effects.
SEMINARS IN CANCER BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Shama Afroz, Zafar Iqbal Warsi, Kahkashan Khatoon, Neelam S. Sangwan, Feroz Khan, Laiq Ur Rahman
Summary: In this study, a key gene CaHMGS in Centella asiatica was successfully isolated and characterized. The gene showed the highest expression level in the roots and the lowest expression level in the node of the plant. Functional complementation experiment confirmed the functionality of the CaHMGS gene. This study contributes to the enhancement of secondary metabolite production in the plant.
MOLECULAR BIOLOGY REPORTS
(2022)
Article
Multidisciplinary Sciences
Jyoti Sangwan, Sandhya Tripathi, Nisha Yadav, Yogesh Kumar, Neelam Sangwan
Summary: SARS-nCoV, a rapidly spreading coronavirus, has affected millions of people worldwide. Understanding the genomic information is crucial for controlling its spread. Comparative sequence analysis revealed variations in the sequences coding for different proteins between SARS CoV and SARS nCoV. The S protein coding sequences of SARS CoV showed deletions, insertions, and substitutions in SARS nCoV sequence. The binding interaction pattern of ACE2 receptor protein with SARS-CoV-2 and Omicron variant indicated potential impacts on virulence.
PROCEEDINGS OF THE INDIAN NATIONAL SCIENCE ACADEMY
(2023)