Crucial steps in the structure determination of a coronavirus spike glycoprotein using cryo-electron microscopy

Resolution advances in cryo-EM enable application to drug discovery

(2016) Sriram Subramaniam et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

Cryo-electron microscopy structure of a coronavirus spike glycoprotein trimer

(2016) Alexandra C. Walls et al.   NATURE

Atomic-accuracy models from 4.5-Å cryo-electron microscopy data with density-guided iterative local refinement

(2015) Frank DiMaio et al.   NATURE METHODS

De novo protein structure determination from near-atomic-resolution cryo-EM maps

(2015) Ray Yu-Ruei Wang et al.   NATURE METHODS

Host cell proteases: Critical determinants of coronavirus tropism and pathogenesis

(2015) Jean Kaoru Millet et al.   VIRUS RESEARCH

2.8 Å resolution reconstruction of the Thermoplasma acidophilum 20S proteasome using cryo-electron microscopy

(2015) Melody G Campbell et al.   eLife

Measuring the optimal exposure for single particle cryo-EM using a 2.6 Å reconstruction of rotavirus VP6

(2015) Timothy Grant et al.   eLife

Sampling the conformational space of the catalytic subunit of human γ-secretase

(2015) Xiao-chen Bai et al.   eLife

Single-particle cryo-EM data acquisition by using direct electron detection camera

(2015) Shenping Wu et al.   Microscopy

Coronaviruses: Important Emerging Human Pathogens

(2014) C. M. Coleman et al.   JOURNAL OF VIROLOGY

Host cell entry of Middle East respiratory syndrome coronavirus after two-step, furin-mediated activation of the spike protein

(2014) Jean Kaoru Millet et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Coronavirus Cell Entry Occurs through the Endo-/Lysosomal Pathway in a Proteolysis-Dependent Manner

(2014) Christine Burkard et al.   PLoS Pathogens

Ambiguities in helical reconstruction

(2014) Edward H Egelman   eLife

Beam-induced motion correction for sub-megadalton cryo-EM particles

(2014) Sjors HW Scheres   eLife

Likelihood-based classification of cryo-EM images using FREALIGN

(2013) Dmitry Lyumkis et al.   JOURNAL OF STRUCTURAL BIOLOGY

Maximizing the potential of electron cryomicroscopy data collected using direct detectors

(2013) David Veesler et al.   JOURNAL OF STRUCTURAL BIOLOGY

Structure of the Fusion Core and Inhibition of Fusion by a Heptad Repeat Peptide Derived from the S Protein of Middle East Respiratory Syndrome Coronavirus

(2013) J. Gao et al.   JOURNAL OF VIROLOGY

Molecular basis of binding between novel human coronavirus MERS-CoV and its receptor CD26

(2013) Guangwen Lu et al.   NATURE

Electron counting and beam-induced motion correction enable near-atomic-resolution single-particle cryo-EM

(2013) Xueming Li et al.   NATURE METHODS

Cryo-EM model validation using independent map reconstructions

(2013) Frank DiMaio et al.   PROTEIN SCIENCE

Structure of RSV Fusion Glycoprotein Trimer Bound to a Prefusion-Specific Neutralizing Antibody

(2013) J. S. McLellan et al.   SCIENCE

High-Resolution Comparative Modeling with RosettaCM

(2013) Yifan Song et al.   STRUCTURE

Ribosome structures to near-atomic resolution from thirty thousand cryo-EM particles

(2013) Xiao-chen Bai et al.   eLife

Crystal Structure of Bovine Coronavirus Spike Protein Lectin Domain

(2012) Guiqing Peng et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

RELION: Implementation of a Bayesian approach to cryo-EM structure determination

(2012) Sjors H.W. Scheres   JOURNAL OF STRUCTURAL BIOLOGY

Movies of Ice-Embedded Particles Enhance Resolution in Electron Cryo-Microscopy

(2012) Melody G. Campbell et al.   STRUCTURE

A Bayesian View on Cryo-EM Structure Determination

(2011) Sjors H.W. Scheres   JOURNAL OF MOLECULAR BIOLOGY

Structure of Respiratory Syncytial Virus Fusion Glycoprotein in the Postfusion Conformation Reveals Preservation of Neutralizing Epitopes

(2011) J. S. McLellan et al.   JOURNAL OF VIROLOGY

Modeling Symmetric Macromolecular Structures in Rosetta3

(2011) Frank DiMaio et al.   PLoS One

Structural basis for immunization with postfusion respiratory syncytial virus fusion F glycoprotein (RSV F) to elicit high neutralizing antibody titers

(2011) K. A. Swanson et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Crystal structure of mouse coronavirus receptor-binding domain complexed with its murine receptor

(2011) G. Peng et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Production and biophysical characterization of the CorA transporter from Methanosarcina mazei

(2009) David Veesler et al.   ANALYTICAL BIOCHEMISTRY

The spike protein of SARS-CoV — a target for vaccine and therapeutic development

(2009) Lanying Du et al.   NATURE REVIEWS MICROBIOLOGY

Crystal structure of NL63 respiratory coronavirus receptor-binding domain complexed with its human receptor

(2009) K. Wu et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Viral membrane fusion

(2008) Stephen C Harrison   NATURE STRUCTURAL & MOLECULAR BIOLOGY