Article
Multidisciplinary Sciences
Alex J. Vecchio, Sewwandi S. Rathnayake, Robert M. Stroud
Summary: The study reveals the molecular and structural basis of how CpE targets susceptible claudins, as well as differences in the primary CpE receptors in mice and humans due to sequence changes in the target motif.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biochemistry & Molecular Biology
Jean L. L. Beltran, Lila G. G. McGrath, Sophia Caruso, Richara K. K. Bain, Claire E. E. Hendrix, Hana Kamran, Hartlee G. G. Johnston, Rebecca M. M. Collings, Menkara-Chinua N. Henry, Tsega-Ab L. Abera, Valeria A. A. Donoso, Erin C. C. Carriker, Bryan H. H. Thurtle-Schmidt
Summary: Borate transporters regulate intracellular borate levels, which are essential for plant growth but toxic in excess. In yeast, borate export confers tolerance. Despite sequence and solute differences, borate transporters share structural homology with human bicarbonate transporters in the SLC4 family. This study characterizes the yeast borate transporter Bor1p and demonstrates shared properties and critical amino acids with SLC4 transporters, providing insights into their mechanisms and functional similarities.
Article
Chemistry, Organic
D. Aerssens, L. Miret-Casals, D. Gomez, D. Sousa-Neves, Y. Levy, M. De Vleesschouwer, A. Manicardi, A. Madder
Summary: We report the co-existence of parallel and antiparallel conformations of E3/K3 and E3/R3 heterodimeric coiled-coil systems in solution for the first time. Introducing a furanylated amino acid in the (EIAALEK)(3) sequence enabled the freezing of the respective coiled-coil complexes through photo-induced covalent crosslinking. Computational simulations and fluorescence experiments based on pyrene-pyrene stacking further confirmed the presence of both conformations in solution.
ORGANIC & BIOMOLECULAR CHEMISTRY
(2023)
Article
Multidisciplinary Sciences
Jiangfeng Zhao, Hao Xie, Ahmad Reza Mehdipour, Schara Safarian, Ulrich Ermler, Cornelia Muenke, Yvonne Thielmann, Gerhard Hummer, Ingo Ebersberger, Jingkang Wang, Hartmut Michel
Summary: MATE transporters utilize H+ or Na+ gradients to export xenobiotics, with the substrate binding site located in the N bundle playing a crucial role in its function. The crystal structure of a MATE transporter from Aquifex aeolicus provides new insights into this important transporter family.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biochemistry & Molecular Biology
Hetao Chen, Hang Zhang, Pu Chen, Song Xiang
Summary: This study elucidated the interaction mechanism between CRTCs and 14-3-3, as well as their crucial roles in nuclear translocation and activation of gene transcription. The discovery of a novel salt bridge structure expanded the understanding of this interaction.
JOURNAL OF MOLECULAR BIOLOGY
(2021)
Article
Biology
Darko Mitrovic, Sarah E. McComas, Claudia Alleva, Marta Bonaccorsi, David Drew, Lucie Delemotte
Summary: In this study, we predicted the structures of the entire sugar porter superfamily in each state of the transport cycle using coevolution analysis and comparative modeling, and identified the molecular determinants of the transport cycle. This research is of great significance for understanding the functional mechanism of transport proteins.
Article
Chemistry, Physical
Remi F. Andre, Gwenaelle Rousse, Capucine Sassoye, Maxim Avdeev, Benedikt Lassalle-Kaiser, Benoit Baptiste, Sophie Carenco
Summary: This article investigates the key compound of cerium oxide as a catalyst and identifies an intermediate phase of Ce-(IV) that forms in the presence of air. The crystal structure of this phase is proposed, and it is confirmed to be an observable intermediate in the synthesis of cerium dioxide.
CHEMISTRY OF MATERIALS
(2023)
Article
Chemistry, Inorganic & Nuclear
A. P. Tyutyunnik, V. D. Zhuravlev, A. Yu Chufarov, I. V. Baklanova, T. A. Patrusheva
Summary: The compound is a polycrystalline orthophosphate Pb0.954(3)Mn2(PO4)(2) obtained at 830 degrees C in a melt with NaCl, which is isostructural to SrMn2(PO4)(2). It has specific structural parameters and a hexagonal close packing of phosphate groups. Lead deficiency is compensated for by a change in the oxidation state of part of manganese.
JOURNAL OF SOLID STATE CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Shruti Chatterjee, Shankar V. Kundapura, Aditya J. Basak, Debangshu Mukherjee, Sagarika Dash, Namrata Ganguli, Amit K. Das, Gayatri Mukherjee, Dibyendu Samanta, Udupi A. Ramagopal
Summary: Tuberculosis is a disease caused by Mycobacterium tuberculosis, primarily transmitted through inhaling droplets from infected individuals. Mycobacteria use pathogen-associated molecular patterns (PAMPs) on their surface to enter alveolar macrophages by recognizing pattern recognition receptors on host cells. One important PAMP is the 19 kDa surface antigen LpqH, which plays a critical role in host-pathogen interactions and immune regulation. This study presents the crystal structure of non-acylated LpqH and demonstrates its functionality in inducing apoptosis in human monocytic cell line THP-1. Conservation analysis reveals a patch of conserved residues on the protein surface, potentially involved in binding partner recognition during host-pathogen interaction.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Multidisciplinary Sciences
Azadeh Shahsavar, Peter Stohler, Gleb Bourenkov, Iwan Zimmermann, Martin Siegrist, Wolfgang Guba, Emmanuel Pinard, Steffen Sinning, Markus A. Seeger, Thomas R. Schneider, Roger J. P. Dawson, Poul Nissen
Summary: This study utilized synthetic single-domain antibodies and serial synchrotron crystallography to determine the structure of GlyT1 in complex with an inhibitor, revealing that the inhibitor locks GlyT1 in an inward-open conformation and binds at the intracellular gate of the release pathway. These findings define the mechanism of inhibition and provide insights for the rational design of new, clinically efficacious GlyT1 inhibitors.
Review
Biophysics
Stephen H. White
Summary: The author shares his childhood experiences in the South, how it shaped his values, his fascination with science, and the influences leading to his higher education. His military service during the Vietnam War enhanced his leadership skills. The majority of the autobiography focuses on his scientific career, emphasizing the contributions of postdoctoral students and colleagues to his lab's progress. White's groundbreaking findings played a significant role in the development of membrane biophysics, highlighting the collaborative and meticulous work required for success in science.
ANNUAL REVIEW OF BIOPHYSICS
(2023)
Article
Biochemistry & Molecular Biology
Robbins Puthenveetil, Eric T. Christenson, Olga Vinogradova
Summary: This review discusses various techniques used in studying protein dynamics, including NMR, cryo-EM, and X-ray crystallography, as well as methods for conformational trapping using specific binders. It also showcases the application of biophysical techniques such as FRET, EPR, and computational approaches in GPCRs, transporters, and ion channels.
Article
Engineering, Environmental
Yang-Peng Lin, Sanlue Hu, Jiahao Xu, Zhizhuang Zhang, Xinghui Qi, Xuexia Lu, Jiance Jin, Xiao-Ying Huang, Qiang Xu, Zhonghua Deng, Zewen Xiao, Ke-Zhao Du
Summary: Zero-dimensional (0D) metal halides based on environmental friendliness, low cost, and earth-abundant elements have gained attention for various applications. In this study, Mn-doped 0D C4H12N2ZnX4 (X = Cl and Br) with strong green emission was developed for scintillator and white light-emitting diode (WLED) applications. Mn doping effect on the electronic structure of C4H12N2ZnX4 was investigated using spectroscopic studies and density functional theory (DFT) calculations. The results showed that Mn doping reduced the bandgap and enhanced the Mn d-d emission, resulting in high photoluminescence quantum yield (PLQY) and luminous efficacy in Mn-doped C4H12N2ZnX4-based WLEDs.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Crystallography
Eya Rezgui, Najoua Ouerfelli, S. R. Gavinho, J. P. F. Carvalho, M. P. F. Graca, S. Soreto Teixeira
Summary: The compounds (Na1.5Mn3MnIII)-Mn-II (0.5)(AsO4) (3) and Na1.5MnII Fe-3(III) (0.5)(AsO4) (3) were synthesized via a high-temperature solid-state combustion reaction and characterized. X-ray diffraction measurements showed their monoclinic crystal structures. These compounds have potential applications due to their unique structural and electrical properties.
Article
Biochemistry & Molecular Biology
Keita Matsuoka, Toru Nakatsu, Hiroaki Kato
Summary: This study reported the crystal structure of the outward-facing state of the CmABCB1 G132V mutant, showing that the conformational change caused by the valine substitution led to an increase in ATPase activity, although the specific mechanism behind this remains unclear.
Article
Biochemistry & Molecular Biology
Timothy J. Wendorff, Bryan H. Schmidt, Pauline Heslop, Caroline A. Austin, James M. Berger
JOURNAL OF MOLECULAR BIOLOGY
(2012)
Article
Multidisciplinary Sciences
Bryan H. Schmidt, Alex B. Burgin, Joseph E. Deweese, Neil Osheroff, James M. Berger
Review
Cell Biology
Seychelle M. Vos, Elsa M. Tretter, Bryan H. Schmidt, James M. Berger
NATURE REVIEWS MOLECULAR CELL BIOLOGY
(2011)
Article
Biochemistry & Molecular Biology
Bryan H. Schmidt, Neil Osheroff, James M. Berger
NATURE STRUCTURAL & MOLECULAR BIOLOGY
(2012)
Article
Biochemistry & Molecular Biology
Kathryn M. Hart, Michael J. Harms, Bryan H. Schmidt, Carolyn Elya, Joseph W. Thornton, Susan Marqusee
Article
Multidisciplinary Sciences
Sebastian Flores, Andrew P. Feld, Bryan H. Thurtle-Schmidt
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2019)
Article
Biochemistry & Molecular Biology
Jean L. L. Beltran, Lila G. G. McGrath, Sophia Caruso, Richara K. K. Bain, Claire E. E. Hendrix, Hana Kamran, Hartlee G. G. Johnston, Rebecca M. M. Collings, Menkara-Chinua N. Henry, Tsega-Ab L. Abera, Valeria A. A. Donoso, Erin C. C. Carriker, Bryan H. H. Thurtle-Schmidt
Summary: Borate transporters regulate intracellular borate levels, which are essential for plant growth but toxic in excess. In yeast, borate export confers tolerance. Despite sequence and solute differences, borate transporters share structural homology with human bicarbonate transporters in the SLC4 family. This study characterizes the yeast borate transporter Bor1p and demonstrates shared properties and critical amino acids with SLC4 transporters, providing insights into their mechanisms and functional similarities.
Article
Multidisciplinary Sciences
Fedor V. Karginov, Cecilia Conaco, Zhenyu Xuan, Bryan H. Schmidt, Joel S. Parker, Gail Mandel, Gregory J. Hannon
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2007)