Generation of Recombinant Monoclonal Antibodies from Immunised Mice and Rabbits via Flow Cytometry and Sorting of Antigen-Specific IgG+ Memory B Cells

Long-lived plasma cells are early and constantly generated in New Zealand Black/New Zealand White F1 mice and their therapeutic depletion requires a combined targeting of autoreactive plasma cells and their precursors

(2015) Adriano Taddeo et al.   ARTHRITIS RESEARCH & THERAPY

Exposure of Epitope Residues on the Outer Face of the Chikungunya Virus Envelope Trimer Determines Antibody Neutralizing Efficacy

(2014) R. H. Fong et al.   JOURNAL OF VIROLOGY

Monoclonal anti-β1-adrenergic receptor antibodies activate G protein signaling in the absence of β-arrestin recruitment

(2013) Catherine J Hutchings et al.   mAbs

The rapid generation of recombinant functional monoclonal antibodies from individual, antigen-specific bone marrow-derived plasma cells isolated using a novel fluorescence-based method

(2013) Alison M Clargo et al.   mAbs

Mammalian cell display for the discovery and optimization of antibody therapeutics

(2013) Peter M. Bowers et al.   METHODS

An automated system for high-throughput single cell-based breeding

(2013) Nobuo Yoshimoto et al.   Scientific Reports

The Discovery, Engineering and Characterisation of a Highly Potent Anti-Human IL-13 Fab Fragment Designed for Administration by Inhalation

(2012) Daniel Lightwood et al.   JOURNAL OF MOLECULAR BIOLOGY

High Affinity Humanized Antibodies without Making Hybridomas; Immunization Paired with Mammalian Cell Display and In Vitro Somatic Hypermutation

(2012) Audrey D. McConnell et al.   PLoS One

A Novel Rabbit Immunospot Array Assay on a Chip Allows for the Rapid Generation of Rabbit Monoclonal Antibodies with High Affinity

(2012) Tatsuhiko Ozawa et al.   PLoS One

Ex vivo characterization and isolation of rare memory B cells with antigen tetramers

(2011) B. Franz et al.   BLOOD

Rapid selection of single cells with high antibody production rates by microwell array

(2011) Sunyoung Park et al.   JOURNAL OF BIOTECHNOLOGY

Beyond natural antibodies: the power of in vitro display technologies

(2011) Andrew R M Bradbury et al.   NATURE BIOTECHNOLOGY

Rapid isolation of antigen-specific antibody-secreting cells using a chip-based immunospot array

(2011) Aishun Jin et al.   Nature Protocols

Single B cell antibody technologies

(2011) Thomas Tiller   New Biotechnology

Plasmablast-derived polyclonal antibody response after influenza vaccination

(2010) Xiao-Song He et al.   JOURNAL OF IMMUNOLOGICAL METHODS

Rapid Generation of Rotavirus-Specific Human Monoclonal Antibodies from Small-Intestinal Mucosa

(2010) R. Di Niro et al.   JOURNAL OF IMMUNOLOGY

Cloning and expression of murine Ig genes from single B cells

(2009) Thomas Tiller et al.   JOURNAL OF IMMUNOLOGICAL METHODS

A rapid and efficient single-cell manipulation method for screening antigen-specific antibody–secreting cells from human peripheral blood

(2009) Aishun Jin et al.   NATURE MEDICINE

Rapid generation of fully human monoclonal antibodies specific to a vaccinating antigen

(2009) Kenneth Smith et al.   Nature Protocols

Screening individual hybridomas by microengraving to discover monoclonal antibodies

(2009) Adebola O Ogunniyi et al.   Nature Protocols

An optimized electrofusion-based protocol for generating virus-specific human monoclonal antibodies

(2008) Xiaocong Yu et al.   JOURNAL OF IMMUNOLOGICAL METHODS

A method for identification of HIV gp140 binding memory B cells in human blood

(2008) Johannes F. Scheid et al.   JOURNAL OF IMMUNOLOGICAL METHODS

Efficient generation of monoclonal antibodies from single human B cells by single cell RT-PCR and expression vector cloning

(2007) Thomas Tiller et al.   JOURNAL OF IMMUNOLOGICAL METHODS