Article
Microbiology
Marcelo L. Merli, Kaylie A. Padgett-Pagliai, Alexandra E. Cuaycal, Lucila Garcia, Maria Rosa Marano, Graciela L. Lorca, Claudio F. Gonzalez
Summary: The study revealed that a potential effector LotP of 'Candidatus Liberibacter asiaticus' may interact with chaperones like GroEL and Hsp40 to enhance Lon protease activity, leading to damage in citrus plant tissues. LotP may also interact with plant proteins, indicating its potential role in protease activity.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Plant Sciences
Bernard Reynaud, Patrick Turpin, Florencia M. Molinari, Martial Grondin, Solene Roque, Frederic Chiroleu, Alberto Fereres, Helene Delatte
Summary: Huanglongbing (HLB) is a serious disease of citrus worldwide, caused by three non-cultivable bacteria. The African psyllid Trioza erytreae is capable of efficiently acquiring and transmitting the bacteria, highlighting the high risk of spread of the disease in Europe.
FRONTIERS IN PLANT SCIENCE
(2022)
Review
Microbiology
Chuanyu Yang, Veronica Ancona
Summary: This review summarizes the main aspects of mechanisms against citrus greening, including inhibition of virulence genes, activation of plant defense genes and metabolites, and the role of citrus microbiome in combatting the disease.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Plant Sciences
Monica N. Alves, Laudecir L. Raiol-Junior, Eduardo A. Girardi, Maeva Miranda, Nelson A. Wulff, Everton Carvalho, Silvio A. Lopes, Jesus A. Ferro, Patrick Ollitrault, Leandro Pena
Summary: Huanglongbing (HLB), the most destructive citrus disease, is associated with the Candidatus Liberibacter species Ca. L. asiaticus (Las). In this study, it was found that certain Oceanian citrus genotypes and hybrids showed full resistance to Las, with their leaves remaining Las-free even when the susceptible rootstock was infected. However, Las was detected in the stem bark above the graft union. Further experiments confirmed that the insects feeding on the Oceanian citrus plants and their canopies, new flushes, and leaves from rooted cuttings remained negative for Las, and their budwood pieces were unable to infect sweet orange through grafting.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Agronomy
Minli Bao, Zheng Zheng, Jianchi Chen, Xiaoling Deng
Summary: Huanglongbing (HLB) is a devastating disease affecting citrus production worldwide, and in China it is associated with the unculturable alpha-proteobacterium Candidatus Liberibacter asiaticus (CLas). Phages/prophages of CLas have been identified through genomic research, aiding in understanding CLas biology. This study investigated the symptom development of different citrus cultivars infected with CLas strains carrying Type 1 and Type 2 phages, providing baseline information for future research on the roles of CLas phages in HLB symptom development.
Article
Plant Sciences
Monica N. Alves, Silvio A. Lopes, Laudecir L. Raiol-Junior, Nelson A. Wulff, Eduardo A. Girardi, Patrick Ollitrault, Leandro Pena
Summary: Huanglongbing (HLB) is the most destructive and incurable disease of citrus, with no resistant citrus genotypes currently available. Some citrus relatives have been identified as fully resistant to HLB, but they are phylogenetically distant from citrus. By evaluating a diverse collection of graft-compatible Citrinae species, new potential sources of resistance to HLB were identified, including Eremocitrus glauca and Papua/New Guinea Microcitrus species.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Plant Sciences
Pan Shen, Xueyi Li, Shimin Fu, Changyong Zhou, Xuefeng Wang
Summary: This study found that a peptide called SECP8 secreted by the citrus huanglongbing bacterium CLas has the ability to suppress immune responses and promote disease development. It also affects the expression of immune-related genes.
FRONTIERS IN PLANT SCIENCE
(2022)
Review
Microbiology
Bin Hu, Muhammad Junaid Rao, Xiuxin Deng, Sheo Shankar Pandey, Connor Hendrich, Fang Ding, Nian Wang, Qiang Xu
Summary: Citrus Huanglongbing (HLB), also known as citrus greening, is a devastating citrus disease with no commercial citrus cultivars resistant to it. The most prevalent strain associated with HLB, Candidatus Liberibacter asiaticus (CLas), has yet to be cultured in vitro. Research has focused on the complex pathosystem of Ca. Liberibacter, including the interaction of host and CLas, and the impact of CLas infection on host transcripts, proteins, and metabolism. Identifying candidate genes for CLas pathogenicity and improving HLB tolerance in citrus are key research areas for the future.
Article
Microbiology
Rui-Xu Jiang, Feng Shang, Hong-Bo Jiang, Wei Dou, Tomislav Cernava, Jin-Jun Wang
Summary: This study investigates the impact of the Candidatus Liberibacter asiaticus (CLas) pathogenic bacteria on the bacterial community in different color morphs, genders, and tissues of the Asian citrus psyllid (ACP). The results show that there are no significant differences in bacterial community diversity and CLas acquired ratio between different color morphs and genders of ACP adults. However, acquiring CLas promotes the diversity and richness of the adult bacterial community. CLas also changes the bacterial structure in different tissues, making the bacterial relationship network simpler than in an uninfected condition. These findings provide essential knowledge on ACP symbionts and the effects of CLas on the ACP bacterial community.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Plant Sciences
Jiaquan Huang, Iobana Alanis-Martinez, Lucita Kumagai, Zehan Dai, Zheng Zheng, Adalberto A. Perez de Leon, Jianchi Chen, Xiaoling Deng
Summary: This study showed that there were at least two different introductions of CLas into Mexico, and the CLas strains between Mexico and the USA are closely related. Two Geno-groups were established, which provide the basis for future CLas subspecies research.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Subhas Hajeri, Sandra Olkowski, Lucita Kumagai, Neil Mcroberts, Raymond K. Yokomi
Summary: Early detection and prompt response are crucial in eradicating 'huanglongbing' in California. A study found that testing the peduncle tissue of fruit consistently resulted in higher detection rates of the disease compared to other tissue types.
Article
Plant Sciences
Jiao Du, Qiying Wang, Chunhua Zeng, Changyong Zhou, Xuefeng Wang
Summary: A newly discovered secretory protein, AGH17470, associated with Huanglongbing, has been found to trigger plant immune response, promote growth and development, and enhance resistance to pathogens.
MOLECULAR PLANT PATHOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Haoqi Wang, Nirmitee Mulgaonkar, Samavath Mallawarachchi, Manikandan Ramasamy, Carmen S. Padilla, Sonia Irigoyen, Gitta Coaker, Kranthi K. Mandadi, Sandun Fernando
Summary: This study screened antimicrobial peptides that have the potential to inhibit the growth of citrus greening bacteria. The findings were further validated by in vitro experiments and the binding mechanism was analyzed. These findings are important for designing effective therapies to manage plant diseases associated with citrus greening.
Article
Microbiology
Jiaquan Huang, Zehan Dai, Zheng Zheng, Priscila A. da Silvia, Luci Kumagai, Qijun Xiang, Jianchi Chen, Xiaoling Deng
Summary: This study analyzed HLB-related bacteria in Asian citrus psyllid and citrus samples from southern California using metagenomics, and developed a metagenomic pipeline for future research. Some bacteria identified, such as Bradyrhizobium and Mesorhizobium, were found to potentially interfere with common CLas detection methods in low or zero titer situations.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Plant Sciences
Laudecir L. Raiol-Junior, Juan C. Cifuentes-Arenas, Nik J. Cunniffe, Robert Turgeon, Silvio A. Lopes
Summary: The study found that the movement speed of Las in plants is between 2.9-3.8 cm per day, and the colonization of plants by Las is influenced by temperature, with 25.7 degrees Celsius being the optimal condition. This explains the higher impact and incidence of huanglongbing disease during the winter months or regions of milder climates in Brazil.
Article
Forestry
Ute Albrecht, Indu Tripathi, Hoyoun Kim, Kim D. Bowman
TREES-STRUCTURE AND FUNCTION
(2019)
Article
Forestry
Ute Albrecht, Indu Tripathi, Kim D. Bowman
TREES-STRUCTURE AND FUNCTION
(2020)
Article
Ecology
Andrea Nuzzo, Aditi Satpute, Ute Albrecht, Sarah L. Strauss
Article
Agronomy
Kim D. Bowman, Ute Albrecht
Article
Forestry
Antonio Castellano-Hinojosa, Bo Meyering, Andrea Nuzzo, Sarah L. Strauss, Ute Albrecht
Summary: Plant biostimulants did not improve citrus health and productivity in an HLB-endemic environment. Only fulvic acids showed significant increases in root and leaf macro- and micronutrient concentrations, which were linked to changes in specific bacterial and fungal taxa in the citrus rhizosphere. Long-term and higher application rates of fulvic acids may be needed to have measurable effects on tree health and productivity in HLB-endemic conditions. Different management practices and soil and environmental conditions can influence the efficacy of biostimulants on root and tree health.
TREES-STRUCTURE AND FUNCTION
(2021)
Article
Agronomy
Susmita Gaire, Ute Albrecht, Ozgur Batuman, Jawwad Qureshi, Mongi Zekri, Fernando Alferez
Summary: In Florida, protecting young trees from ACP and CLas infection is crucial due to the prevalence of ACP and HLB. The use of IPCs can effectively exclude psyllids and prevent CLas infection, but may also lead to the incidence of other diseases and pests.
Article
Agronomy
Leigh Archer, Jawwad Qureshi, Ute Albrecht
Summary: Trunk injection is an effective method for managing huanglongbing (HLB) in citrus, with different compounds showing varying efficacy. Imidacloprid injection reduces psyllid populations, but its effectiveness diminishes over time. Oxytetracycline injection improves tree health, reduces bacterial titers, and decreases fruit drop, leading to increased yield.
Article
Plant Sciences
Leigh Archer, Sanju Kunwar, Fernando Alferez, Ozgur Batuman, Ute Albrecht
Summary: This study evaluated the effects of trunk injection of oxytetracycline (OTC) on the management of Huanglongbing (HLB) in citrus trees. The injection resulted in reduced fruit drop, increased yield and fruit size, and improved juice quality. However, the effectiveness varied depending on the timing of injection and the citrus variety.
Article
Horticulture
Sudip Kunwar, Bo Meyering, Jude Grosser, Fred G. Gmitter, William S. Castle, Ute Albrecht
Summary: Huanglongbing (HLB), also known as citrus greening disease, has significantly reduced Florida citrus production and acreage since its discovery in 2005. There is currently no cure for this disease, increasing the demand for HLB-tolerant rootstocks. This study compared diploid and tetraploid rootstocks for their effects on tree size, yield, and tolerance to HLB.
SCIENTIA HORTICULTURAE
(2023)
Article
Forestry
Leigh Archer, Ute Albrecht
Summary: Trunk injection causes injury to trees and best practices must be established for using this technology to manage vascular diseases. This study found that injection during spring and summer promotes faster wound healing compared to injection in fall or winter, and injection into the scion is less damaging than injection into the rootstock. The study provides insights on the best practices for utilizing trunk injection in citrus as a crop protection strategy.
TREES-STRUCTURE AND FUNCTION
(2023)
Review
Horticulture
Leigh Archer, Jonathan H. Crane, Ute Albrecht
Summary: Trunk injection is a targeted method of delivering pesticides, nutrients, and other materials directly into the stem or trunk of plants. It is used for disease and pest management in high-value forest trees, ornamental plants, and commercial agricultural crops when other application methods are ineffective or pose environmental hazards.
Article
Infectious Diseases
Nabil Killiny, Pedro Gonzalez-Blanco, Yulica Santos-Ortega, Fuad Al-Rimawi, Amit Levy, Faraj Hijaz, Ute Albrecht, Ozgur Batuman
Article
Plant Sciences
Wanli You, Jinglin Zhang, Xueyin Ru, Feng Xu, Zhengguo Wu, Peng Jin, Yonghua Zheng, Shifeng Cao
Summary: This study investigated the effect of calcium chloride (CaCl2) treatment on GABA accumulation in fresh-cut cantaloupe and the underlying mechanisms. The results showed that CaCl2 treatment increased GABA content and the activities of GAD and SSADH enzymes, while reducing glutamate content and GABA-T activity. Additionally, CaCl2 treatment upregulated the expressions of CmCML11 and CmCAMTA5, as well as several GABA shunt genes, through the transcriptional activation by CmCAMTA5. Furthermore, the interaction between CmCML11 and CmCAMTA5 enhanced the transcriptional activation of GABA shunt genes. Overall, this study reveals that CaCl2 treatment promotes GABA accumulation in fresh-cut cantaloupe through the combined effect of CmCML11 and CmCAMTA5 in regulating the expressions of GABA shunt genes.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2024)
Article
Plant Sciences
Min Wang, Yupeng Wang, Xiaohui Wang, Guangwei Wei, Huiyi Yang, Xi Yang, Tinghai Shen, Huijie Qu, Sheng Fang, Ziming Wu
Summary: This study identified the high nitrogen use efficiency (NUE) black sesame variety 17-156 and analyzed its underlying physiological and molecular mechanisms. The results showed that 17-156 possesses a sophisticated nitrogen metabolizing machinery to uptake and assimilate higher quantities of inorganic nitrogen, simultaneously improving carbon metabolism and growth. Many important genes were up-regulated in 17-156 under high nitrogen condition. Additionally, 38 potential candidate genes were identified for future studies to improve sesame's NUE. These findings provide valuable resources for understanding the regulatory network of nitrogen metabolism and developing sesame cultivars with improved NUE.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2024)
Article
Plant Sciences
He Dong, Chongmei Xu, Chengtao Zhang, Li Zhang, Yaqin Yao, Suiqi Zhang
Summary: The study found that short cells in maize leaves not only improve leaf mechanical support and photosynthetic performance, enhance drought resistance, but also participate in stomatal regulation.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2024)
Article
Plant Sciences
Na Liu, Wenyan Shang, Mengxin Guan, Jibin Xiao, Guangxiang Tian, Baozhan Ma, Wenjing Shang, Xu Li, Shijia Zhao, Chuang Li, Kun Cheng, Wenming Zheng
Summary: This study cloned the full-length cDNA sequence of TaSPX3 gene in wheat and found that TaSPX3 responds to low phosphorus stress in multiple wheat genotypes. Overexpressing TaSPX3 can alleviate phosphorus deficiency symptoms and promote plant growth in Arabidopsis. The study also revealed the interaction of TaSPX3 with other genes related to the phosphorus starvation signaling pathway.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2024)
Article
Plant Sciences
Kongyuan Wu, Lizhen Wang, Zihan Wu, Ziqing Liu, Zengfei Li, Jun Shen, Shengjie Shi, Hong Liu, Christopher Rensing, Renwei Feng
Summary: Selenium (Se) can reduce uptake and translocation of cadmium (Cd) in plants by regulating root morphology. This study investigated the effects of Se(IV) on root exudates, root morphology, root endogenous hormones, and Cd uptake efficiency in rice under Cd stress. The results showed that Se(IV) significantly reduced Cd concentrations in shoots and roots, and decreased Cd uptake efficiency via root hairs. Se(IV) also affected root morphology, root exudates, and the synthesis of hormones like IAA and JA. However, transcriptome analysis revealed no upregulated differentially expressed genes (DEGs) in IAA synthesis.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2024)
Article
Plant Sciences
Di Wang, Huaifang Zhang, Xuefei Hu, Haizhen Zhang, Shuang Feng, Aimin Zhou
Summary: This study identified a cell number regulator gene called SlCNR8 in willow, which enhances resistance to trace metals in transgenic poplar seedlings. SlCNR8 reduces Cd uptake and accumulation, and can be used as a candidate gene for genetic improvement of phytostabilisation of trace metals.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2024)