Use of TALEs and TALEN Technology for Genetic Improvement of Plants

Targeted Editing of Myostatin Gene in Sheep by Transcription Activator-like Effector Nucleases

(2016) Xinxia Zhao et al.   ASIAN-AUSTRALASIAN JOURNAL OF ANIMAL SCIENCES

TALEN-Mediated Homologous Recombination Produces Site-Directed DNA Base Change and Herbicide-Resistant Rice

(2016) Ting Li et al.   Journal of Genetics and Genomics

DNA-guided genome editing using the Natronobacterium gregoryi Argonaute

(2016) Feng Gao et al.   NATURE BIOTECHNOLOGY

TALEN-mediated functional correction of X-linked chronic granulomatous disease in patient-derived induced pluripotent stem cells

(2015) Anne-Kathrin Dreyer et al.   BIOMATERIALS

Tailor-made TALEN system for highly efficient targeted gene replacement in the rice blast fungus

(2015) Takayuki Arazoe et al.   BIOTECHNOLOGY AND BIOENGINEERING

Non-transgenic Plant Genome Editing Using Purified Sequence-Specific Nucleases

(2015) Song Luo et al.   Molecular Plant

Unbiased detection of off-target cleavage by CRISPR-Cas9 and TALENs using integrase-defective lentiviral vectors

(2015) Xiaoling Wang et al.   NATURE BIOTECHNOLOGY

Epigenome editing by a CRISPR-Cas9-based acetyltransferase activates genes from promoters and enhancers

(2015) Isaac B Hilton et al.   NATURE BIOTECHNOLOGY

mRNA transfection of a novel TAL effector nuclease (TALEN) facilitates efficient knockout of HIV co-receptor CCR5

(2015) U. Mock et al.   NUCLEIC ACIDS RESEARCH

Creation of fragrant rice by targeted knockout of theOsBADH2gene using TALEN technology

(2015) Qiwei Shan et al.   PLANT BIOTECHNOLOGY JOURNAL

Heritable site-specific mutagenesis using TALENs in maize

(2015) Si Nian Char et al.   PLANT BIOTECHNOLOGY JOURNAL

Multiplexed, targeted gene editing inNicotiana benthamianafor glyco-engineering and monoclonal antibody production

(2015) Jin Li et al.   PLANT BIOTECHNOLOGY JOURNAL

Artificial TALE as a Convenient Protein Platform for Engineering Broad-Spectrum Resistance to Begomoviruses

(2015) Xiaofei Cheng et al.   Viruses-Basel

Direct regulation of E-cadherin by targeted histone methylation of TALE-SET fusion protein in cancer cells

(2015) Hyun-Soo Cho et al.   Oncotarget

Whole Organism Genome Editing: Targeted Large DNA Insertion via ObLiGaRe Nonhomologous End-Joining in Vivo Capture

(2015) Yutaka Yamamoto et al.   G3-Genes Genomes Genetics

Germline-Transmitted Genome Editing in Arabidopsis thaliana Using TAL-Effector-Nucleases

(2015) Joachim Forner et al.   PLoS One

TALE activators regulate gene expression in a position- and strand-dependent manner in mammalian cells

(2014) Claudia Uhde-Stone et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

Complete decoding of TAL effectors for DNA recognition

(2014) Junjiao Yang et al.   CELL RESEARCH

TALEN-Mediated Generation and Genetic Correction of Disease-Specific Human Induced Pluripotent Stem Cells

(2014) Sivaprakash Ramalingam et al.   CURRENT GENE THERAPY

TALEN Knockout of the PSIP1 Gene in Human Cells: Analyses of HIV-1 Replication and Allosteric Integrase Inhibitor Mechanism

(2014) H. J. Fadel et al.   JOURNAL OF VIROLOGY

Inhibition of DNA Replication of Human Papillomavirus by Using Zinc Finger–Single-Chain FokI Dimer Hybrid

(2014) Takashi Mino et al.   MOLECULAR BIOTECHNOLOGY

Improving CRISPR-Cas nuclease specificity using truncated guide RNAs

(2014) Yanfang Fu et al.   NATURE BIOTECHNOLOGY

Dimeric CRISPR RNA-guided FokI nucleases for highly specific genome editing

(2014) Shengdar Q Tsai et al.   NATURE BIOTECHNOLOGY

Simultaneous editing of three homoeoalleles in hexaploid bread wheat confers heritable resistance to powdery mildew

(2014) Yanpeng Wang et al.   NATURE BIOTECHNOLOGY

Genome-wide analysis reveals characteristics of off-target sites bound by the Cas9 endonuclease

(2014) Cem Kuscu et al.   NATURE BIOTECHNOLOGY

Efficient genome modification by CRISPR-Cas9 nickase with minimal off-target effects

(2014) Bin Shen et al.   NATURE METHODS

Modes of TAL effector-mediated repression

(2014) Jeannette Werner et al.   NUCLEIC ACIDS RESEARCH

Comprehensive analysis of the specificity of transcription activator-like effector nucleases

(2014) Alexandre Juillerat et al.   NUCLEIC ACIDS RESEARCH

Comparison of TALE designer transcription factors and the CRISPR/dCas9 in regulation of gene expression by targeting enhancers

(2014) Xuefei Gao et al.   NUCLEIC ACIDS RESEARCH

Programmable DNA-binding proteins from Burkholderia provide a fresh perspective on the TALE-like repeat domain

(2014) O. d. Lange et al.   NUCLEIC ACIDS RESEARCH

SAPTA: a new design tool for improving TALE nuclease activity

(2014) Yanni Lin et al.   NUCLEIC ACIDS RESEARCH

CHOPCHOP: a CRISPR/Cas9 and TALEN web tool for genome editing

(2014) Tessa G. Montague et al.   NUCLEIC ACIDS RESEARCH

Improved soybean oil quality by targeted mutagenesis of the fatty acid desaturase 2 gene family

(2014) William Haun et al.   PLANT BIOTECHNOLOGY JOURNAL

DNA Replicons for Plant Genome Engineering

(2014) N. J. Baltes et al.   PLANT CELL

Cell-Penetrating Peptide-Mediated Delivery of TALEN Proteins via Bioconjugation for Genome Engineering

(2014) Jia Liu et al.   PLoS One

True-Breeding Targeted Gene Knock-Out in Barley Using Designer TALE-Nuclease in Haploid Cells

(2014) Maia Gurushidze et al.   PLoS One

Seamless modification of wild-type induced pluripotent stem cells to the natural CCR5Δ32 mutation confers resistance to HIV infection

(2014) Lin Ye et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Plant Genome Engineering with Sequence-Specific Nucleases

(2013) Daniel F. Voytas   Annual Review of Plant Biology

TALENoffer: genome-wide TALEN off-target prediction

(2013) Jan Grau et al.   BIOINFORMATICS

Mojo Hand, a TALEN design tool for genome editing applications

(2013) Kevin L Neff et al.   BMC BIOINFORMATICS

Double Nicking by RNA-Guided CRISPR Cas9 for Enhanced Genome Editing Specificity

(2013) F. Ann Ran et al.   CELL

Efficient TALEN construction and evaluation methods for human cell and animal applications

(2013) Tetsushi Sakuma et al.   GENES TO CELLS

Efficient drug screening and gene correction for treating liver disease using patient-specific stem cells

(2013) Su Mi Choi et al.   HEPATOLOGY

Site-Specific Gene Targeting Using Transcription Activator-Like Effector (TALE)-Based Nuclease inBrassica oleracea

(2013) Zijian Sun et al.   Journal of Integrative Plant Biology

Characterization and DNA-Binding Specificities of Ralstonia TAL-Like Effectors

(2013) Lixin Li et al.   Molecular Plant

Rapid and Efficient Gene Modification in Rice and Brachypodium Using TALENs

(2013) Qiwei Shan et al.   Molecular Plant

Inactivation of Hepatitis B Virus Replication in Cultured Cells and In Vivo with Engineered Transcription Activator-Like Effector Nucleases

(2013) Kristie Bloom et al.   MOLECULAR THERAPY

An Efficient Antiviral Strategy for Targeting Hepatitis B Virus Genome Using Transcription Activator-Like Effector Nucleases

(2013) Jieliang Chen et al.   MOLECULAR THERAPY

Targeted DNA demethylation and activation of endogenous genes using programmable TALE-TET1 fusion proteins

(2013) Morgan L Maeder et al.   NATURE BIOTECHNOLOGY

CAS9 transcriptional activators for target specificity screening and paired nickases for cooperative genome engineering

(2013) Prashant Mali et al.   NATURE BIOTECHNOLOGY

Locus-specific editing of histone modifications at endogenous enhancers

(2013) Eric M Mendenhall et al.   NATURE BIOTECHNOLOGY

Conditional targeted genome editing using somatically expressed TALENs in C. elegans

(2013) Ze Cheng et al.   NATURE BIOTECHNOLOGY

Generation of targeted mouse mutants by embryo microinjection of TALEN mRNA

(2013) Benedikt Wefers et al.   Nature Protocols

Advanced genetic tools for plant biotechnology

(2013) Wusheng Liu et al.   NATURE REVIEWS GENETICS

E-TALEN: a web tool to design TALENs for genome engineering

(2013) Florian Heigwer et al.   NUCLEIC ACIDS RESEARCH

megaTALs: a rare-cleaving nuclease architecture for therapeutic genome engineering

(2013) Sandrine Boissel et al.   NUCLEIC ACIDS RESEARCH

Directed evolution of the TALE N-terminal domain for recognition of all 5′ bases

(2013) Brian M. Lamb et al.   NUCLEIC ACIDS RESEARCH

A large-scale in vivo analysis reveals that TALENs are significantly more mutagenic than ZFNs generated using context-dependent assembly

(2013) Shijia Chen et al.   NUCLEIC ACIDS RESEARCH

Targeted molecular trait stacking in cotton through targeted double-strand break induction

(2013) Kathleen D'Halluin et al.   PLANT BIOTECHNOLOGY JOURNAL

TAL effector nucleases induce mutations at a pre-selected location in the genome of primary barley transformants

(2013) Toni Wendt et al.   PLANT MOLECULAR BIOLOGY

Site Specific Mutation of the Zic2 Locus by Microinjection of TALEN mRNA in Mouse CD1, C3H and C57BL/6J Oocytes

(2013) Benjamin Davies et al.   PLoS One

ULtiMATE System for Rapid Assembly of Customized TAL Effectors

(2013) Junjiao Yang et al.   PLoS One

Targeted Gene Deletion of miRNAs in Mice by TALEN System

(2013) Shuji Takada et al.   PLoS One

TAL effectors: highly adaptable phytobacterial virulence factors and readily engineered DNA-targeting proteins

(2013) Erin L. Doyle et al.   TRENDS IN CELL BIOLOGY

Dissecting the Roles of miR-302/367 Cluster in Cellular Reprogramming Using TALE-based Repressor and TALEN

(2013) Zhonghui Zhang et al.   Stem Cell Reports

A TALEN Genome-Editing System for Generating Human Stem Cell-Based Disease Models

(2012) Qiurong Ding et al.   Cell Stem Cell

Obligate Ligation-Gated Recombination (ObLiGaRe): Custom-designed nuclease-mediated targeted integration through nonhomologous end joining

(2012) M. Maresca et al.   GENOME RESEARCH

Rapid and Cost-Effective Gene Targeting in Rat Embryonic Stem Cells by TALENs

(2012) Chang Tong et al.   Journal of Genetics and Genomics

Tell Me a Tale of TALEs

(2012) Alejandra Muñoz Bodnar et al.   MOLECULAR BIOTECHNOLOGY

Inhibition of Binding of Tomato Yellow Leaf Curl Virus Rep to its Replication Origin by Artificial Zinc-Finger Protein

(2012) Tomoaki Mori et al.   MOLECULAR BIOTECHNOLOGY

TALE1 fromXanthomonas axonopodispv.manihotisacts as a transcriptional activator in plant cells and is important for pathogenicity in cassava plants

(2012) Luisa F. Castiblanco et al.   MOLECULAR PLANT PATHOLOGY

Tiptoeing around transgenics

(2012) Emily Waltz   NATURE BIOTECHNOLOGY

High-efficiency TALEN-based gene editing produces disease-resistant rice

(2012) Ting Li et al.   NATURE BIOTECHNOLOGY

TAL effector RVD specificities and efficiencies

(2012) Jana Streubel et al.   NATURE BIOTECHNOLOGY

A ligation-independent cloning technique for high-throughput assembly of transcription activator–like effector genes

(2012) Jonathan L Schmid-Burgk et al.   NATURE BIOTECHNOLOGY

A transcription activator-like effector toolbox for genome engineering

(2012) Neville E Sanjana et al.   Nature Protocols

Chimeric TALE recombinases with programmable DNA sequence specificity

(2012) Andrew C. Mercer et al.   NUCLEIC ACIDS RESEARCH

TAL Effector-Nucleotide Targeter (TALE-NT) 2.0: tools for TAL effector design and target prediction

(2012) Erin L. Doyle et al.   NUCLEIC ACIDS RESEARCH

Iterative capped assembly: rapid and scalable synthesis of repeat-module DNA such as TAL effectors from individual monomers

(2012) Adrian W. Briggs et al.   NUCLEIC ACIDS RESEARCH

Engineering synthetic TAL effectors with orthogonal target sites

(2012) Abhishek Garg et al.   NUCLEIC ACIDS RESEARCH

Transcription Activator-Like Effector Nucleases Enable Efficient Plant Genome Engineering

(2012) Y. Zhang et al.   PLANT PHYSIOLOGY

Rational Diversification of a Promoter Providing Fine-Tuned Expression and Orthogonal Regulation for Synthetic Biology

(2012) Benjamin A. Blount et al.   PLoS One

The Crystal Structure of TAL Effector PthXo1 Bound to Its DNA Target

(2012) A. N.-S. Mak et al.   SCIENCE

Structural Basis for Sequence-Specific Recognition of DNA by TAL Effectors

(2012) D. Deng et al.   SCIENCE

Comprehensive interrogation of natural TALE DNA-binding modules and transcriptional repressor domains

(2012) Le Cong et al.   Nature Communications

Generation of Isogenic Pluripotent Stem Cells Differing Exclusively at Two Early Onset Parkinson Point Mutations

(2011) Frank Soldner et al.   CELL

TAL effectors are remote controls for gene activation

(2011) Heidi Scholze et al.   CURRENT OPINION IN MICROBIOLOGY

Transcriptional Characteristics of Xa21-mediated Defense Responses in Rice

(2011) Qiang Gan et al.   Journal of Integrative Plant Biology

Heritable gene targeting in zebrafish using customized TALENs

(2011) Peng Huang et al.   NATURE BIOTECHNOLOGY

Genetic engineering of human pluripotent cells using TALE nucleases

(2011) Dirk Hockemeyer et al.   NATURE BIOTECHNOLOGY

TALEs of genome targeting

(2011) Jens Boch   NATURE BIOTECHNOLOGY

Efficient construction of sequence-specific TAL effectors for modulating mammalian transcription

(2011) Feng Zhang et al.   NATURE BIOTECHNOLOGY

Targeted gene disruption in somatic zebrafish cells using engineered TALENs

(2011) Jeffry D Sander et al.   NATURE BIOTECHNOLOGY

Knockout rats generated by embryo microinjection of TALENs

(2011) Laurent Tesson et al.   NATURE BIOTECHNOLOGY

Efficient design and assembly of custom TALEN and other TAL effector-based constructs for DNA targeting

(2011) Tomas Cermak et al.   NUCLEIC ACIDS RESEARCH

Assembly of custom TALE-type DNA binding domains by modular cloning

(2011) Robert Morbitzer et al.   NUCLEIC ACIDS RESEARCH

Modularly assembled designer TAL effector nucleases for targeted gene knockout and gene replacement in eukaryotes

(2011) Ting Li et al.   NUCLEIC ACIDS RESEARCH

A novel TALE nuclease scaffold enables high genome editing activity in combination with low toxicity

(2011) Claudio Mussolino et al.   NUCLEIC ACIDS RESEARCH

Targeted transcriptional repression using a chimeric TALE-SRDX repressor protein

(2011) Magdy M. Mahfouz et al.   PLANT MOLECULAR BIOLOGY

Assembly of Designer TAL Effectors by Golden Gate Cloning

(2011) Ernst Weber et al.   PLoS One

Human Umbilical Cord Blood Cells Restore Brain Damage Induced Changes in Rat Somatosensory Cortex

(2011) Maren Geißler et al.   PLoS One

De novo-engineered transcription activator-like effector (TALE) hybrid nuclease with novel DNA binding specificity creates double-strand breaks

(2011) M. M. Mahfouz et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

TAL Effectors: Customizable Proteins for DNA Targeting

(2011) A. J. Bogdanove et al.   SCIENCE

XanthomonasAvrBs3 Family-Type III Effectors: Discovery and Function

(2010) Jens Boch et al.   Annual Review of Phytopathology

TAL effectors: finding plant genes for disease and defense

(2010) Adam J Bogdanove et al.   CURRENT OPINION IN PLANT BIOLOGY

Targeting DNA Double-Strand Breaks with TAL Effector Nucleases

(2010) M. Christian et al.   GENETICS

A TALE nuclease architecture for efficient genome editing

(2010) Jeffrey C Miller et al.   NATURE BIOTECHNOLOGY

NHEJ and its backup pathways in chromosomal translocations

(2010) Michael R Lieber   NATURE STRUCTURAL & MOLECULAR BIOLOGY

ZiFiT (Zinc Finger Targeter): an updated zinc finger engineering tool

(2010) J. D. Sander et al.   NUCLEIC ACIDS RESEARCH

Regulation of selected genome loci using de novo-engineered transcription activator-like effector (TALE)-type transcription factors

(2010) R. Morbitzer et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

TAL effector-DNA specificity

(2010) Heidi Scholze et al.   Virulence

The type III effectors ofXanthomonas

(2009) FRANK F. WHITE et al.   MOLECULAR PLANT PATHOLOGY

A single plant resistance gene promoter engineered to recognize multiple TAL effectors from disparate pathogens

(2009) P. Romer et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Breaking the Code of DNA Binding Specificity of TAL-Type III Effectors

(2009) J. Boch et al.   SCIENCE

A Simple Cipher Governs DNA Recognition by TAL Effectors

(2009) M. J. Moscou et al.   SCIENCE

Zinc-finger nucleases: a powerful tool for genetic engineering of animals

(2009) Séverine Rémy et al.   TRANSGENIC RESEARCH

Manipulation of the Eukaryotic Transcriptional Machinery by Bacterial Pathogens

(2008) Yusuke Saijo et al.   Cell Host & Microbe

Zinc-finger Nucleases: The Next Generation Emerges

(2008) Toni Cathomen et al.   MOLECULAR THERAPY

A One Pot, One Step, Precision Cloning Method with High Throughput Capability

(2008) Carola Engler et al.   PLoS One