Article
Genetics & Heredity
Bin Sun, Xiaorui Ding, Junhua Ye, Yuting Dai, Can Cheng, Jihua Zhou, Fuan Niu, Rongjian Tu, Qiyan Hu, Kaizhen Xie, Yue Qiu, Hongyu Li, Zhizun Feng, Chenbing Shao, Liming Cao, Anpeng Zhang, Huangwei Chu
Summary: In this study, a DH population was generated using the AC method in rice breeding. Through single- and two-locus SD analysis, five segregation distortion loci (SDLs) potentially linked to anther culturability were identified. Among these, SDL5 exhibited an overrepresentation of alleles from the female parent, while SDL1.1, SDL1.2, SDL2, and SDL7 displayed an overrepresentation of alleles from the male parent. Additionally, six pairs of epistatic interactions (EPIs) that influenced two-locus SDs in the DH population were discovered. The findings provide insights into the genetic control of anther culturability in rice.
Article
Plant Sciences
Csaba Lantos, Mihaly Jancso, Arpad Szekely, Eva Nagy, Timea Szaloki, Janos Pauk
Summary: Doubled haploid (DH) plant production, such as anther culture (AC), is a useful tool in modern rice breeding. This study compared the effects of different combinations of plant growth regulators on callus induction in rice, and identified the best combination for in vitro green plant production.
Article
Plant Sciences
Csaba Lantos, Mihaly Jancso, Arpad Szekely, Timea Szaloki, Shoba Venkatanagappa, Janos Pauk
Summary: An efficient protocol for in vitro anther culture in rice breeding, especially for indica genotypes, was evaluated. Three different induction media (N6NDK, N(6)NDZ, Ali-1) and four plant regeneration media (mMSNBK1, MSNBK3, MSNBKZ1, MSNBKZ2) were tested to increase the efficiency of in vitro androgenesis. The production of calli and green plantlets varied among different genotypes and media combinations. Flow cytometric analyses showed that 51.89% of the acclimatized indica rice plantlets had undergone spontaneous chromosome doubling. The utilization of DH plants has been integrated into the Hungarian rice breeding program, and tetraploid lines offer potential for future research and breeding directions.
Article
Forestry
Caixia Liu, Sui Wang, Yi Liu, Meng Wang, Erqin Fan, Chen Liu, Shikai Zhang, Chuanping Yang, Junhui Wang, Heike W. W. Sederoff, Xiangling You, Vincent L. L. Chiang, Su Chen, Ronald R. R. Sederoff, Guanzheng Qu
Summary: In this study, an effective protocol for inducing haploid poplar plants was established, resulting in the successful generation of 96 whole doubled haploid plant lines. The doubled haploid population exhibited significantly higher phenotypic variance compared to the half-sib progeny of the donor tree. Some of the homozygous lines showed excellent potential as model plants for genetic and breeding studies.
JOURNAL OF FORESTRY RESEARCH
(2023)
Review
Biotechnology & Applied Microbiology
Madhu Patial, Ruchi Chauhan, Harinder K. Chaudhary, Kallol K. Pramanick, Arun K. Shukla, Vishnu Kumar, Ramesh P. S. Verma
Summary: The development and improvement of biotechnological tools have greatly contributed to the progress in barley breeding program. The use of doubled haploids has significantly shortened the cultivar development time and has been widely used in various genetic studies. However, challenges such as low efficiency and genotypic specificity still need to be addressed.
CRITICAL REVIEWS IN BIOTECHNOLOGY
(2023)
Article
Plant Sciences
Samira Tajedini, Baratali Fakheri, Mohsen Niazian, Nafiseh Mahdinezhad, Abdelbagi Mukhtar Ali Ghanim, Ali Kazemi Pour, Ivan Ingelbrecht, Mehran E. Shariatpanahi
Summary: Two different androgenesis pathways, shed-microspore culture (SMC) and anther culture (AC), were developed for haploid induction in mutagenized M-1 rice cultivars. The effects of various factors on induction and regeneration phases were evaluated. The SMC method showed better results in terms of the number of regenerated embryos. Chromosome counting and flow cytometry analysis confirmed the presence of haploid plants, providing a valuable foundation for rice breeding research.
JOURNAL OF PLANT GROWTH REGULATION
(2023)
Article
Multidisciplinary Sciences
Cucu Gunarsih, Bambang Sapta Purwoko, Iswari Saraswati Dewi, Willy Bayuardi Suwarno, Nafisah
Summary: This study aimed to evaluate the anther culture ability and doubled-haploid plants production of F(1) populations derived from indica rice parents with single or multiple abiotic stress tolerance. The results indicated that the F(1) population derived from HS4-11-1-2/B13926E-KA-23 showed higher ability in anther culture compared to other populations. The developed doubled-haploid lines need further evaluation and screening to identify high-yielding and abiotic stress tolerant individuals.
Article
Multidisciplinary Sciences
Byomkesh Dash, Sudhansu Sekhar Bhuyan, Sandeep Kumar Singh, Manjusha Chandravani, Nibedita Swain, Prachitara Rout, Jawahar Lal Katara, C. Parameswaran, B. N. Devanna, Sanghamitra Samantaray
Summary: Rice is crucial for global food security. This study developed an androgenic protocol for indica rice, contributing to yield improvement.
Review
Biology
Ricardo Mir, Antonio Calabuig-Serna, Jose M. Segui-Simarro
Summary: This review compiles the latest advances in doubled haploid plant production, the application of doubled haploid lines in breeding programs, and future perspectives for alternative technologies for doubled haploid generation in eggplant. Eggplant is globally cultivated for its edible fruit, and breeding programs aim to produce F1 hybrids by crossing highly homozygous pure lines. Doubled haploid individuals can be induced from haploid cells in a single generation, offering a more efficient alternative to traditional self crossing methods.
Article
Biotechnology & Applied Microbiology
Swapnil Ravindra Kulkarni, S. M. Balachandran, K. Ulaganathan, Divya Balakrishnan, A. S. Hari Prasad, G. Rekha, M. B. V. N. Kousik, S. K. Hajira, Ravindra Ramarao Kale, D. Aleena, M. Anila, E. Punniakoti, T. Dilip, K. Pranathi, M. Ayyappa Das, Mastanbee Shaik, K. Chaitra, Pragya Sinha, R. M. Sundaram
Summary: A DH population from the rice hybrid KRH-2 was used for QTL mapping to identify novel genomic regions associated with yield traits, resulting in the identification of both major and minor effect QTLs. Chromosomal regions with gene clusters were found on chromosomes 3 and 6, while most identified QTLs co-localized with previously reported regions. Five new major effect QTLs and three minor effect QTLs associated with yield traits were discovered, providing potential targets for breeding programs.
Article
Plant Sciences
Paul Chege, Erzsebet Kiss, Csaba Lantos, Andrea Palagyi, Janos Pauk
Summary: The study aimed at producing doubled haploids of sorghum successfully from five genotypes, leading to the development of an improved protocol. Temperature and light conditions were found to play a crucial role in the successful process of regeneration and rooting, with sorghum's high tillering ability and abiotic stress tolerance observed to contribute to the attainment of haploid plantlets.
PHYTON-INTERNATIONAL JOURNAL OF EXPERIMENTAL BOTANY
(2021)
Article
Multidisciplinary Sciences
Ramandeep Singh, Ruma Devi, Navraj Kaur Sarao
Summary: Hybrids of cauliflower are in high demand worldwide due to their desirable traits. Traditional hybrid development requires multiple generations of inbreeding, while doubled haploid technology allows for the generation of completely homozygous inbred lines in a single step. A study was conducted to develop a protocol for doubled haploid lines in cauliflower. Different genotypes and media compositions were tested for callus induction and shoot regeneration. Ploidy analysis revealed the presence of haploids, spontaneous doubled haploids, tetraploids, and mixoploids.
Article
Plant Sciences
Pavel Kostylev, Nataliya Kalinina, Nataliya Vozhzhova, Valentina Golubova, Natalya Chertkova
Summary: Flood resistance is crucial in controlling weeds in rice cultivation. Currently, there are no suitable varieties in Russia. This study aimed to create second-generation rice hybrids with resistance to prolonged flooding by crossing economically valuable varieties with genes for flood resistance. Molecular genetic evaluation and growth energy assessment were conducted to identify resistant plants. The study successfully identified green regenerative lines with genotypic differences and high potential for growth under deep flooding.
Review
Biotechnology & Applied Microbiology
Serik Eliby, Sara Bekkuzhina, Olena Kishchenko, Gulnur Iskakova, Gulnar Kylyshbayeva, Satyvaldy Jatayev, Kathleen Soole, Peter Langridge, Nikolai Borisjuk, Yuri Shavrukov
Summary: Doubled haploid production is a valuable biotechnology that accelerates the breeding of new wheat varieties and plays important roles in studying genetic control, marker-assisted selection, genomics, and genetic engineering in wheat. This paper reviews recent advances in androgenesis and gynogenesis techniques, with a focus on the in vitro culture phase, and the emerging innovative approaches in researching and producing wheat doubled haploids. The use of CRISPR-based genome editing for inducing doubled haploids in wheat is also discussed.
BIOTECHNOLOGY ADVANCES
(2022)
Article
Agronomy
So-Myeong Lee, Nkulu Rolly Kabange, Ju-Won Kang, Youngho Kwon, Jin-Kyung Cha, Hyeonjin Park, Ki-Won Oh, Jeonghwan Seo, Hee-Jong Koh, Jong-Hee Lee
Summary: In this study, QTLs controlling grain filling in rice were investigated using KASP markers in a DH population derived from a cross between indica and japonica varieties. Three significant QTLs associated with grain filling were identified, and genes associated with diverse biological processes and molecular functions were found. Genes such as MYB, WRKY60, and OsSh1 played a key role in controlling grain filling under early cultivation conditions.
Article
Multidisciplinary Sciences
Manoj Kumar Yadav, S. Aravindan, Umakanta Ngangkham, S. Raghu, S. R. Prabhukarthikeyan, U. Keerthana, B. C. Marndi, Totan Adak, Susmita Munda, Rupesh Deshmukh, D. Pramesh, Sanghamitra Samantaray, P. C. Rath
Correction
Multidisciplinary Sciences
Manoj Kumar Yadav, S. Aravindan, Umakanta Ngangkham, S. Raghu, S. R. Prabhukarthikeyan, U. Keerthana, B. C. Marndi, Totan Adak, Susmita Munda, Rupesh Deshmukh, D. Pramesh, Sanghamitra Samantaray, P. C. Rath
Article
Food Science & Technology
Awadhesh Kumar, Darshan Panda, Monalisha Biswal, Prajjal Dey, Lambodar Behera, Mirja Jaynul Baig, Lopamudra Nayak, Umakanta Ngangkham, Srigopal Sharma
Article
Agronomy
Umakanta Ngangkham, Swamp Kumar Parida, Ashok Kumar Singh, Trilochan Mohapatra
Article
Biochemistry & Molecular Biology
Jawahar Lal Katara, Ram Lakhan Verma, Madhuchhanda Parida, Umakanta Ngangkham, Kutubuddin Ali Molla, Kalyani Makarand Barbadikar, Mitadru Mukherjee, C. Parameswaran, Sanghamitra Samantaray, Nageswara Rao Ravi, Onkar Nath Singh, Trilochan Mohapatra
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Article
Food Science & Technology
Awadhesh Kumar, Puja Archana Panda, Milan Kumar Lal, Umakanta Ngangkham, Chandrasekhar Sahu, Khela Ram Soren, Hata Nath Subudhi, Sanghamitra Samantaray, Srigopal Sharma
Article
Agronomy
Parameswaran Chidambaranathan, Cayalvizhi Balasubramaniasai, Niranjana Behura, Mohini Purty, Sanghamitra Samantaray, Hatanath Subudhi, Umakanta Ngangkham, B. N. Devanna, Jawahar Lal Katara, Awadhesh Kumar, Lambodar Behera
Summary: Rice varieties were evaluated for heat tolerance during the dry season, with 67 selected varieties validated in the second dry season. Some varieties showed good tolerance to heat stress, reducing spikelet sterility. The allelic phenotypic effect of favorable alleles played a crucial role in improving heat tolerance in rice varieties.
GENETIC RESOURCES AND CROP EVOLUTION
(2021)
Article
Biotechnology & Applied Microbiology
H. Sharanabasav, D. Pramesh, M. K. Prasannakumar, E. Chidanandappa, M. K. Yadav, U. Ngangkham, B. Parivallal, B. T. Raghavendra, C. Manjunatha, S. K. Sharma, N. Karthik
Summary: The study characterized the geo-distinct isolates of Ustilaginoidea virens for morpho-molecular and mating-type locus diversity, revealing significant diversity among isolates from Southern India and distribution of heterothallic mating types in paddy fields. Complete synteny conservation between Indian and Chinese strains was observed at the MAT1 locus.
JOURNAL OF APPLIED MICROBIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Chittaragi Amoghavarsha, Devanna Pramesh, Ganesh R. Naik, Manjunath K. Naik, Manoj K. Yadav, Umakanta Ngangkham, Eranna Chidanandappa, Adke Raghunandana, Huded Sharanabasav, Siddepalli E. Manjunatha
Summary: The study investigated the diversity of eco-distinct isolates of Magnaporthe oryzae in southern India in terms of morphological, virulence, and molecular characteristics, as well as the distribution of five Avr genes. PCR analysis showed that Avr-Piz-t, Avr-Pik, and Avr-Pia were predominant among the isolates. The research provides valuable information for plant breeders to develop blast disease-resistant rice cultivars.
JOURNAL OF APPLIED MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Awadhesh Kumar, Sarangadhar Nayak, Umakanta Ngangkham, Rameswar Prasad Sah, Milan Kumar Lal, T. P. Azharudheen, Sasmita Behera, Padmini Swain, Lambodar Behera, Srigopal Sharma
Summary: The flow of phosphorus in agricultural land is influenced by various factors, with phytic acid being the main storage form of phosphorus in rice grains. Genetic variations can affect the accumulation of phosphorus and phytic acid in grains, ultimately impacting mineral bioavailability.
JOURNAL OF FOOD BIOCHEMISTRY
(2021)
Article
Biotechnology & Applied Microbiology
Amoghavarsha Chittaragi, Devanna Pramesh, Ganesha R. Naik, Manjunath K. Naik, Manoj K. Yadav, Umakanta Ngangkham, Manjunatha E. Siddepalli, Anusha Nayak, Muthukapalli K. Prasannakumar, Chidanandappa Eranna
Summary: This study investigates the genetic diversity, population structure, and mating-type distribution of Magnaporthe oryzae isolates associated with leaf blast disease of rice in Karnataka, India. The results reveal a genetically homogenous population of M. oryzae with two mating types. The study highlights the importance of understanding the population structure and sexual mating behavior of M. oryzae for developing region-specific blast-resistant rice cultivars.
JOURNAL OF APPLIED MICROBIOLOGY
(2022)
Article
Plant Sciences
Umakanta Ngangkham, Sonali Nanda, Jawahar Lal Katara, Sanghamitra Samantaray
Summary: Seed germination in rice is highly sensitive to salinity stress, with significant variation observed in tolerant and susceptible lines. Through a study using 117 DH lines, genomic regions linked to salt tolerance during germination were identified from the candidate genes. Further research is recommended to explore the mechanisms of salinity tolerance during seed germination in rice.
INDIAN JOURNAL OF GENETICS AND PLANT BREEDING
(2021)
Article
Biotechnology & Applied Microbiology
Umakanta Ngangkham, Jawahar Lal Katara, P. S. Shanmugavadivel, Manoj Kumar Yadav, Suresh Yadav, Nongthombam Devachandra, Sanghamitra Samantaray, Lotan Kumar Bose
INDIAN JOURNAL OF BIOTECHNOLOGY
(2020)
Article
Plant Sciences
Madhuchhanda Parida, Umakanta Ngangkham, Jawahar Lal Katara, Manoj Kumar Yadav, Sanghamitra Samantaray, Trilochan Mohapatra
INDIAN JOURNAL OF GENETICS AND PLANT BREEDING
(2020)
Article
Multidisciplinary Sciences
Krishnendu Chattopadhyay, Lambodar Behera, Torit Baran Bagchi, Sushree Sibanee Sardar, Nutan Moharana, Niraja Rani Patra, Mridul Chakraborti, Avijit Das, Bishnu Charan Marndi, Ananta Sarkar, Umakanta Ngangkham, Koushik Chakraborty, Lotan Kumar Bose, Sutapa Sarkar, Soham Ray, Srigopal Sharma
SCIENTIFIC REPORTS
(2019)