Article
Genetics & Heredity
Solmaz Najafi, Mehmet Ulker, Erol Oral, Ruveyde Tuncturk, Murat Tuncturk, R. Z. Sayyed, Kahkashan Perveen, Peter Poczai, Andras Cseh
Summary: This study evaluated cytogenetic analyses in different Aegilops species using nuclear DNA quantification. The results showed significant differences in DNA content among diploid and tetraploid species in Turkey and Iran, but not in hexaploid species. Intraspecific genome size variation was low, while interspecific variation was significant. The flow cytometry technique allowed for easy and accurate differentiation of Aegilops species.
Article
Plant Sciences
Tao Fujiwara, Hongmei Liu, Esteban Meza-Torres, Rita E. Morero, Alvaro J. Vega, Zhenlong Liang, Atsushi Ebihara, Ilia J. Leitch, Harald Schneider
Summary: By recording parameters related to genome size and chromosome number, we demonstrated the importance of genome diversity and disparity in shaping fern species diversity.
Article
Biochemistry & Molecular Biology
Pol Fernandez, Ilia J. Leitch, Andrew R. Leitch, Oriane Hidalgo, Maarten J. M. Christenhusz, Lisa Pokorny, Jaume Pellicer
Summary: Giant genomes are rare in the plant kingdom, with most research focusing on angiosperms and gymnosperms. However, a study on ferns reveals differences in genome organization and dynamics. The genus Tmesipteris has two species with giant genomes, which exhibit high repeat diversity compared to small-genome taxa.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Plant Sciences
Pol Fernandez, Oriane Hidalgo, Ana Juan, Ilia J. Leitch, Andrew R. Leitch, Luis Palazzesi, Luca Pegoraro, Juan Viruel, Jaume Pellicer
Summary: Polyploidy is a common phenomenon in angiosperms and plays a significant role in plant diversification. This study focused on the population-level cytotype screening of Senecio doronicum and found the coexistence of tetraploid, hexaploid, and octoploid individuals. The analysis of genomic data supports the autopolyploid origin of these polyploid individuals and suggests the contribution of tetraploids in the formation of hexaploids. The coexistence of different cytotypes may indicate the initiation of speciation.
Article
Genetics & Heredity
Zuzana Chumova, Terezie Mandakova, Pavel Travnicek
Summary: Polyploidy plays a crucial role in plant evolution, with some species having limited understanding of their evolutionary history. This study used various techniques to investigate the origins of sweet vernal grass and alpine sweet vernal grass, revealing different polyploidization patterns and extensive variation among European populations.
Article
Multidisciplinary Sciences
Susann Plath, Evelyn Klocke, Thomas Nothnagel
Summary: Asparagus wild relatives have the potential to enhance the genetic variability and develop new cultivars with desirable traits in garden asparagus. A detailed cytogenetic characterization of these resources is necessary for their efficient utilization in breeding.
Article
Plant Sciences
Natalia Borowska-Zuchowska, Magdalena Senderowicz, Dana Trunova, Bozena Kolano
Summary: Cytogenetics is a branch of genetics focused on cellular components, especially chromosomes, and their relationship to heredity, genome structure, function, and evolution. Modern cytogenetic approaches allow for the visualization of different sequences in the plant genome and contribute to understanding its evolutionary history.
Review
Genetics & Heredity
Shruthi Balachandra, Sharanya Sarkar, Amanda A. Amodeo
Summary: Cell maintains a relatively constant nuclear-to-cytoplasmic ratio by coupling DNA content, nuclear size, and cell size, which plays a crucial role in cell growth and cycle progression to meet physiological demands.
ANNUAL REVIEW OF GENETICS
(2022)
Article
Evolutionary Biology
Kara M. Million, Amrita Bhattacharya, Zoe M. Dinges, Sarah Montgomery, Eries Smith, Curtis M. Lively
Summary: Research suggests that clonal populations of the New Zealand freshwater snail maintain high levels of single-nucleotide polymorphism (SNP) diversity and DNA content variation even without sexual reproduction. Laboratory experiments also reveal differences in pathogen susceptibility among distinct clonal genotypes.
JOURNAL OF HEREDITY
(2021)
Article
Evolutionary Biology
Trevor J. Krabbenhoft, Daniel J. MacGuigan, Nathan J. C. Backenstose, Hannah Waterman, Tianying Lan, Jessie A. Pelosi, Milton Tan, Simen R. Sandve
Summary: The study on the genome of the Chinese sucker revealed a catostomid-specific whole-genome duplication event, with strong conserved synteny and approximately even distribution of gene content and repetitive elements across homeologous chromosomes. This duplication event provides an independent example of genome evolution following WGD in animals, showing extreme end of conserved genome architecture over at least 25.2 million years.
GENOME BIOLOGY AND EVOLUTION
(2021)
Article
Biochemistry & Molecular Biology
Kelly E. Miller, Clotilde Cadart, Rebecca Heald
Summary: Genome size and cell size in frogs are strongly correlated, and this correlation affects developmental rate. However, it is unclear how the relationship between cell size and ploidy is established during embryonic development. By studying different polyploid frogs, researchers discovered that cell size is primarily determined by egg size, while nuclear size is correlated with genome size. At the subcellular level, nuclear size is more strongly correlated with genome size, while mitotic spindle size scales with cell size. These findings demonstrate the relationship between cell size and ploidy in frogs and reveal different size scaling mechanisms during embryogenesis, suggesting that Xenopus development is consistent across a wide range of genome and egg sizes.
Article
Biochemistry & Molecular Biology
Delphine Giraud, Oscar Lima, Virginie Huteau, Olivier Coriton, Julien Boutte, Ales Kovarik, Andrew R. Leitch, Ilia J. Leitch, Malika Ainouche, Armel Salmon
Summary: Repeated sequences and polyploidy are essential in plant genome dynamics. This study analyzed the evolutionary dynamics of repeats in different ploidy levels of Spartina species over the past 10 million years, finding that genetic variations among species are mainly due to divergence times rather than ploidy changes. The research also suggests that certain repeat sequences may have an impact on chromosome evolution.
Article
Cell Biology
Xiaowei Yan, Nico Stuurman, Susana A. Ribeiro, Marvin E. Tanenbaum, Max A. Horlbeck, Christina R. Liem, Marco Jost, Jonathan S. Weissman, Ronald D. Vale
Summary: A microscopy-based optical enrichment approach was developed for selecting and marking cells displaying specific CRISPR-induced phenotypes, followed by isolation using fluorescence-activated cell sorting (FAG). Automated phenotypic identification and photoactivation of cells were achieved through a plugin developed for the open source software mu Manager, allowing screening of approximately 1.5 million individual cells in 8 hours. This scalable approach facilitated the identification of 14 bona fide hits out of 6,092 sgRNAs targeting 544 genes in terms of their effects on nuclear size regulation.
JOURNAL OF CELL BIOLOGY
(2021)
Article
Developmental Biology
Vijay Pratap Singh, Huzaifa Hassan, Fengyan Deng, Dai Tsuchiya, Sean McKinney, Kevin Ferro, Jennifer L. Gerton
Summary: This paper explores the role of polyploidy in the murine placenta and identifies Myc as a key regulator of polyploidy and placental development. The study shows that Myc is essential for multiple rounds of DNA replication, supports gene expression involved in replication and nucleotide biosynthesis, and prevents DNA damage and premature senescence in trophoblast giant cells.
Article
Agronomy
Manqing Li, Miaoli Wang, Jingxue Zhang, Guilan Feng, Maryam Noor, Zhipeng Guo, Yuxia Guo, Yongzhuo Guan, Xuebing Yan
Summary: Understanding the cytological pattern of genome size and ploidy level of bermudagrass is essential for studying its evolution pattern and breeding. This study measured the genome size and ploidy level of bermudagrass along a longitudinal gradient and explored their relationship with climate factors. The tetraploid ploidy level was the most common, and aneuploidy was frequently observed in different regions.
Article
Biochemistry & Molecular Biology
Paula Burchardt, Tatiana T. Souza-Chies, Olivier Chauveau, Sidia M. Callegari-Jacques, Lauis Brisolara-Correa, Camila Dellanhese Inacio, Lilian Eggers, Sonja Siljak-Yakovlev, Jose Marcello Salabert de Campos, Eliane Kaltchuk-Santos
GENETICS AND MOLECULAR BIOLOGY
(2018)
Article
Biochemistry & Molecular Biology
Juliana Lustosa Matos de Alencar, Eliane Kaltchuk-Santos, Juliana Fachinetto, Luana Olinda Tacuatia, Eliana Regina Forni-Martins, Eudes Maria Stiehl-Alves, Tatiana Teixeira de Souza-Chies
GENETICS AND MOLECULAR BIOLOGY
(2018)
Article
Plant Sciences
Lilian Eggers, Rafaella Marchioretto, Tatiana T. Souza-Chies, Olivier Chauveau
ACTA BOTANICA BRASILICA
(2019)
Article
Plant Sciences
Ana Paula Moraes, Mohammad Vatanparast, Caroline Polido, Andre Marques, Gustavo Souza, Ana Paula Fortuna-Perez, Eliana R. Forni-Martins
BRAZILIAN JOURNAL OF BOTANY
(2020)
Article
Biochemistry & Molecular Biology
Raquel Moura Machado, Fernanda Ancelmo de Oliveira, Fabio de Matos Alves, Anete Pereira de Souza, Eliana Regina Forni-Martins
Summary: Polyploidy is a significant evolutionary force in plant diversification by altering population genetic diversity. The study isolated and characterized nuclear microsatellite markers of P. cattleyanum, which showed high transferability to related species. The newly designed SSR markers revealed insights into genetic diversity and population structure, contributing to future evolutionary studies in natural polyploids.
BIOCHEMICAL GENETICS
(2021)
Article
Plant Sciences
Leonardo N. da Silva, Jeffery M. Saarela, Liliana Essi, Tatiana T. de Souza-Chies
Summary: This study evaluated the circumscription of Calothecinae and the infrageneric classification of Chascolytrum, proposing adjustments based on phylogenetic analysis with comprehensive sampling. The results showed that Calothecinae and Chascolytrum are not monophyletic as currently recognized, and suggested new classifications for Trisetum brasiliense and Trisetum bulbosum. Laegaardia and Paramochloa were identified as sister taxa to Chascolytrum, leading to the establishment of a new subtribe Paramochloinae. Additionally, the study provided a more resolved phylogeny of Chascolytrum and proposed the division of Chascolytrum into nine genera, including two new ones.
JOURNAL OF SYSTEMATICS AND EVOLUTION
(2022)
Article
Plant Sciences
Maria Romero-da-Cruz, Rafael Guimaraes, Marcelo F. Devecchi, Jose R. Pirani, Eliana R. Forni-Martins
Summary: The study conducted the first cytogenetic analysis of Homalolepis and reconstructed the evolution of chromosome numbers in the Simaroubaceae family by surveying chromosome numbers of other representatives. The results showed that Homalolepis species have 2n = 32 chromosome numbers, with several CMA(+) bands and diverse distribution of ribosomal DNA sites among different species.
BRAZILIAN JOURNAL OF BOTANY
(2022)
Review
Plant Sciences
Rafael Guimaraes, Eliana Regina Forni-Martins
Summary: Sapindales consists of six larger families and three smaller families, with controversies surrounding their inter-family relations and chromosome numbers. There is a great diversity of chromosome numbers within each family, with Rutaceae and Sapindaceae having the highest percentages of species with known chromosome counts. Polyploidy played a significant role in the diversification of large families, particularly in Meliaceae and Rutaceae. Dysploidy is also frequent in the diversification of the large families.
BRAZILIAN JOURNAL OF BOTANY
(2022)
Article
Plant Sciences
Veronica A. Thode, Camila Dellanhese Inacio, Lilian Eggers, Marcelo Reginato, Tatiana T. Souza-Chies
Summary: The evolution of Sisyrinchium in the Americas involved origins in the Mid-Miocene in the Andes and Mesoamerica, followed by early diversification at high elevations and later colonization of lower elevations in the Pliocene/Pleistocene. Sympatric speciation in south-eastern Brazil and long-dispersal events to North America were observed. Climate changes and shifts in elevational ranges influenced diversification in Sisyrinchium.
BOTANICAL JOURNAL OF THE LINNEAN SOCIETY
(2022)
Article
Forestry
Raquel Moura Machado, Fernanda Ancelmo de Oliveira, Ana Carolina Devides Castello, Fabio de Matos Alves, Anete Pereira de Souza, Eliana Regina Forni-Martins
Summary: This study uses Psidium cattleyanum as a research subject, and through the analysis of genetic data of natural populations and climate niche analysis, it confirms two hypotheses about speciation in polyploids promoted by polyploidization and provides new information on the genetic diversity and ecological niches affected by polyploidy.
TREE GENETICS & GENOMES
(2022)
Article
Plant Sciences
Raquel Moura Machado, Eliana Regina Forni-Martins
Summary: This study provides cytogenetic data on Psidium cattleyanum and identifies nine cytotypes, including two new records. The chromosome counts, CMA/DAPI bands, and rDNA sites increased linearly with ploidy level, indicating the multiplication of the same genome. However, the cytotypes 2n = 110 and 132 showed lower DNA content than expected. There was a weak relationship between ploidy and latitudinal distribution.
BRAZILIAN JOURNAL OF BOTANY
(2022)
Review
Plant Sciences
Suiane Santos Oleques, Tatiana Teixeira de Souza-Chies, Rubem Samuel de Avila Jr
Summary: Grassland ecosystems in South Brazil exhibit unique patterns of plant-pollinator interactions, with bees playing a significant role as pollinators. However, other pollinator groups and plant species in these grasslands have received limited research attention, particularly nocturnal pollinators, flies, beetles, and birds. There is a need for more detailed studies to further understand the diversity and maintenance of grassland floras in South Brazil.
ACTA BOTANICA BRASILICA
(2021)
Article
Chemistry, Medicinal
Mauricio Barancelli Schwedersky, Marina Scopel, Luciana R. Tallini, Jaume Bastida, Tatiana T. Souza-Chies, Nora H. Oleas, Jose Angelo Silveira Zuanazzi
REVISTA BRASILEIRA DE FARMACOGNOSIA-BRAZILIAN JOURNAL OF PHARMACOGNOSY
(2020)
Article
Evolutionary Biology
Janaina Gomes-da-Silva, Tatiana T. Souza-Chies
Article
Plant Sciences
Alexandre Cristante Martins, Rafaella Migliavacca Marchioretto, Ariane Tonetto Vieira, Eudes Maria Stiehl-Alves, Eliane Kaltchuk dos Santos, Tatiana Teixeira de Souza-Chies
ACTA BOTANICA BRASILICA
(2020)