Article
Biochemistry & Molecular Biology
Anna Signorile, Anna Ferretta, Consiglia Pacelli, Nazzareno Capitanio, Paola Tanzarella, Maria Laura Matrella, Alessio Valletti, Domenico De Rasmo, Tiziana Cocco
Summary: Parkin plays a crucial role in mitochondrial function and calcium balance. Treatment with resveratrol in Parkin-mutant fibroblasts can lead to increased Ca2+ level, modulation of cAMP homeostasis, and restoration of protein expression involved in ER-mitochondria contact points.
Article
Multidisciplinary Sciences
Yong Bhum Song, Seung-Yeol Park, Kunyou Park, Hayoung Hwang, Rona S. Carroll, Victor W. Hsu, Ursula B. Kaiser
Summary: G protein-coupled receptors (GPCRs) play crucial roles in physiological and pathological processes, and mutations can lead to diseases. This study focuses on PROKR2 and its mutant causing hypogonadotropic hypogonadism, and identifies distinct interactomes and post-ER itinerary that determine the fate of the wild-type and mutant receptors. The study also reveals the role of the post-ER itinerary in reducing ER stress induced by mutant PROKR2.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Multidisciplinary Sciences
Mate Katona, Adam Bartok, Zuzana Nichtova, Gyorgy Csordas, Elena Berezhnaya, David Weaver, Arijita Ghosh, Peter Varnai, David Yule, Gyorgy Hajnoczky
Summary: The study reveals that the IP3 receptor traffics in and out of ER-Mitochondria contacts, and when trapped, enhances calcium signaling to stimulate energy metabolism.
NATURE COMMUNICATIONS
(2022)
Article
Microbiology
Liping Peng, Jiawen Du, Runfan Zhang, Nali Zhu, He Zhao, Qiang Zhao, Qilin Yu, Mingchun Li
Summary: The study found that the growth and cell vitality of the Δcnb1/Delta/Delta yvc1/Delta/Delta mutant were better than the Δcnb1/Delta/Delta strain. Under ER stress, the cellular calcium concentration decreased and fluctuation was weakened in the Δcnb1/Delta/Delta yvc1/Delta/Delta strain.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Multidisciplinary Sciences
Shanliang Zheng, Dong Zhao, Guixue Hou, Song Zhao, Wenxin Zhang, Xingwen Wang, Li Li, Liang Lin, Tie-Shan Tang, Ying Hu
Summary: The study demonstrates the interaction between TMCO1 and iASPP in colon cancer, affecting tumor growth and cell apoptosis by modulating Ca2+ homeostasis. Intervention in the iASPP-TMCO1 axis can effectively inhibit tumor progression.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Review
Cell Biology
Jody Groenendyk, Wen-An Wang, Alison Robinson, Marek Michalak
Summary: Calreticulin is a crucial protein for cardiac development and maintenance of proper cardiac function. It supports Ca2+-dependent signaling and enhances the efficiency of cardiomyocytes.
Article
Cell Biology
Benjamin Cartes-Saavedra, Josefa Macuada, Daniel Lagos, Duxan Arancibia, Maria E. Andres, Patrick Yu-Wai-Man, Gyoergy Hajnoczky, Veronica Eisner
Summary: Autosomal Dominant Optic Atrophy (ADOA), caused by OPA1 mutations, affects Ca2+ homeostasis by modulating ER-mitochondria coupling, potentially contributing to the progression of ADOA.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Cell Biology
Muhammad Rosyid Ridlo, Geon A. Kim, Anukul Taweechaipaisankul, Eui Hyun Kim, Byeong Chun Lee
Summary: Research showed that zinc supplementation during in vitro maturation can alleviate ER stress, improve nuclear maturation of oocytes, and enhance normal embryonic development.
JOURNAL OF CELLULAR PHYSIOLOGY
(2021)
Article
Multidisciplinary Sciences
Lauren C. Panzera, Ben Johnson, Josiah A. Quinn, In Ha Cho, Michael M. Tamkun, Michael B. Hoppa
Summary: Research indicates that Kv2.1 channels play a crucial role in facilitating ER calcium uptake during electrical activity in neurons, which is related to synaptic transmission. Loss of the Kv2.1 channel can disrupt synaptic vesicle fusion during stimulation, while its non-conducting role can link ER calcium uptake with electrical activity.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Neurosciences
Mohammad A. Ghane, Wei Wei, Dina W. Yakout, Zachary D. Allen, Cassandra L. Miller, Bin Dong, Jenny J. Yang, Ning Fang, Angela M. Mabb
Summary: The study found that disrupting Arc protein ubiquitination enhances DHPG-induced ER-mediated Ca2+ release. This alteration is observed in all neuronal subregions except secondary branchpoints. Disruptions in Arc ubiquitination increase Arc interaction with the integral ER protein Calnexin.
FRONTIERS IN CELLULAR NEUROSCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Lauren Y. Shields, Huihui Li, Kevin Nguyen, Hwajin Kim, Zak Doric, Joseph H. Garcia, T. Michael Gill, Dominik Haddad, Keith Vossel, Meredith Calvert, Ken Nakamura
Summary: The loss of central mitochondrial fission protein Drp1 worsens learning and memory in mice expressing mutant hAPP in neurons, leading to mitochondrial Ca2+ overload. This suggests a potential mechanism by which mitochondrial fission protects against hAPP-driven pathology.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Review
Biochemistry & Molecular Biology
Na Song, Ming Yang, Hao Zhang, Shi-kun Yang
Summary: Kidney disease is a serious health concern, and understanding the pathogenesis is crucial for precise therapies. Disruption of calcium homeostasis plays a fundamental role in kidney disease development and is a promising therapeutic strategy. The endoplasmic reticulum and mitochondria are key organelles, with pharmacologic modulation of cellular calcium homeostasis as a novel treatment approach.
CURRENT MEDICINAL CHEMISTRY
(2021)
Review
Biochemistry & Molecular Biology
Barbara Zablocka, Dariusz C. Gorecki, Krzysztof Zablocki
Summary: Duchenne muscular dystrophy is caused by mutations in the DMD gene, leading to the loss of full-length dystrophin and resulting in muscle degeneration, neuropsychological impairment, and bone deformities. Abnormalities in calcium homeostasis are a common feature of this disease, playing a fundamental role in the various abnormalities associated with DMD.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemical Research Methods
Tito Cali, Marisa Brini
Summary: Membrane contact sites between organelles play a crucial role in cellular homeostasis and can be quantified using SPLICS sensors in both fixed and living cells.
Article
Neurosciences
Alexandre Bouron
Summary: The endoplasmic reticulum (ER) is the primary storage compartment for calcium (Ca2+) in eukaryotic cells. Ca2+ is mobilized from the ER through store-operated Ca2+ channels (SOCCs), leading to a store-operated Ca2+ entry (SOCE) that has been extensively studied in non-excitable cells. The presence of this Ca2+ entry route in neurons has been a subject of debate, but experimental evidence suggests that SOCE can be generated by the recruitment of Ca2+ from neuronal ER Ca2+ stores. This review summarizes the main supporting studies and explores the molecular composition, expression, pharmacological properties, and physiological relevance of neuronal SOCCs.
MOLECULAR NEUROBIOLOGY
(2023)
Article
Plant Sciences
Sakiko Ishida, Hidemasa Suzuki, Aya Iwaki, Shogo Kawamura, Shohei Yamaoka, Mikiko Kojima, Yumiko Takebayashi, Katsushi Yamaguchi, Shuji Shigenobu, Hitoshi Sakakibara, Takayuki Kohchi, Ryuichi Nishihama
Summary: In this study, it was demonstrated that in the liverwort Marchantia polymorpha, the decrease in endogenous auxin level at the cut surface of decapitated explants induced the expression of a key transcription factor gene, MpLAXR. The expression of MpLAXR was able to overcome the inhibition of regeneration by exogenously applied auxin, and ectopic expression of MpLAXR promoted cell proliferation in normally quiescent tissues. These findings provide insights into stem cell regulation and apical dominance establishment in land plants.
PLANT AND CELL PHYSIOLOGY
(2022)
Article
Multidisciplinary Sciences
Shuji Shigenobu, Yoshinobu Hayashi, Dai Watanabe, Gaku Tokuda, Masaru Y. Hojo, Kouhei Toga, Ryota Saiki, Hajime Yaguchi, Yudai Masuoka, Ryutaro Suzuki, Shogo Suzuki, Moe Kimura, Masatoshi Matsunami, Yasuhiro Sugime, Kohei Oguchi, Teruyuki Niimi, Hiroki Gotoh, Masaru K. Hojo, Satoshi Miyazaki, Atsushi Toyoda, Toru Miura, Kiyoto Maekawa
Summary: Termites are social organisms with a polyphenic caste system, and gene duplication plays a crucial role in their social evolution. The duplicated genes in the termite genome are associated with caste-biased gene expression and diverse social functions, contributing to caste-specialized functions. Gene duplication facilitates social evolution through regulatory diversification, leading to caste-biased expression and subfunctionalization and/or neofunctionalization.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Biology
Shuji Shigenobu, Shunta Yorimoto
Summary: This review summarizes recent progress in genomics of aphids and their symbionts, revealing pervasive gene duplication and chromosomal rearrangements, and identifying novel symbionts using modern deep-sequencing technologies.
CURRENT OPINION IN INSECT SCIENCE
(2022)
Article
Biology
Yui Uchida, Shuji Shigenobu, Hiroyuki Takeda, Chikara Furusawa, Naoki Irie
Summary: This study demonstrates that the phylotypic period, characterized by low potential for phenotypic variations, is developmentally stable and evolutionarily conserved. The results suggest that the intrinsic nature of organisms, specifically developmental stability, may limit the phenotypic variations and bias evolutionary outcomes.
Article
Developmental Biology
Takashi Takeuchi, Haruka Matsubara, Fumina Minamitani, Yukio Satoh, Sayo Tozawa, Tomoki Moriyama, Kohei Maruyama, Ken-ichi T. Suzuki, Shuji Shigenobu, Takeshi Inoue, Koji Tamura, Kiyokazu Agata, Toshinori Hayashi
Summary: This study generated mutants of Hox13 genes using the CRISPR/Cas9 system in newts and found that Hox13 genes are essential for digit formation in both development and regeneration. The predominant role of Hoxa13 in newt limbs, unlike in mouse limbs, suggests a potential contribution of Hoxa13 in the transition from fins to limbs. These findings demonstrate the conservation of Hox13 gene function in limb development and regeneration across taxa.
Article
Biochemistry & Molecular Biology
Chen-yo Chung, Shuji Shigenobu
Summary: Differentiation of the non-reproductive caste is a unique feature of eusocial insects, and apoptosis in oocytes plays a major role in constraining their reproductivity. This study investigated the soldier sterility in a hemipteran insect, the social aphid Ceratovacuna japonica, and found that soldiers possess ovaries and germaria but their ovarioles are small and lacking gastrulating embryos. The study also revealed apoptosis in the maternal nutritive cells of soldier ovaries and developmental failure of embryos.
INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY
(2022)
Article
Evolutionary Biology
Hajime Yaguchi, Shogo Suzuki, Naoto Kanasaki, Yudai Masuoka, Ryutaro Suzuki, Ryouhei H. Suzuki, Yoshinobu Hayashi, Shuji Shigenobu, Kiyoto Maekawa
Summary: In this study, the researchers investigated the role of vitellogenin (Vg) in nonreproductive castes of termites. They found that Vg4 plays an important role in the nonreproductive functions of termites. This suggests that Vg genes have undergone functionalization during the eusocial transition of termites.
JOURNAL OF EXPERIMENTAL ZOOLOGY PART B-MOLECULAR AND DEVELOPMENTAL EVOLUTION
(2023)
Article
Plant Sciences
Ryo Tabata, Takehiro Kamiya, Shunpei Imoto, Hana Tamura, Kumiko Ikuta, Michika Tabata, Tasuku Hirayama, Hironaka Tsukagoshi, Keitaro Tanoi, Takamasa Suzuki, Takushi Hachiya, Hitoshi Sakakibara
Summary: Plants have evolved sophisticated systemic processes to optimize their nutrient acquisition efficiency in heterogeneous soil environments. This study investigates the molecular basis of long-distance signaling in response to iron starvation and reveals the regulatory mechanism of iron homeostasis in plants.
PLANT AND CELL PHYSIOLOGY
(2022)
Article
Multidisciplinary Sciences
Satoshi Ishishita, Shumpei Kitahara, Mayuko Takahashi, Sakura Iwasaki, Shoji Tatsumoto, Izumi Hara, Yoshiki Kaneko, Keiji Kinoshita, Katsushi Yamaguchi, Akihito Harada, Yasushige Ohmori, Yasuyuki Ohkawa, Yasuhiro Go, Shuji Shigenobu, Yoichi Matsuda, Takayuki Suzuki
Summary: This study investigated the molecular basis of low protoporphyrin IX (PPIX) production in the uterus of a white eggshell phenotype in Japanese quails, using transcriptome analysis and genetic linkage analysis. The results showed that several genes involved in heme biosynthesis, including ALAS1, HEPHL1, and PITX3, were specifically upregulated in the wild-type uterus and downregulated in the mutant. Further biochemical analysis indicated that the restricted availability of 5'-aminolevulinic acid is the main cause of low PPIX production. These findings suggest that uterus-specific transcriptional regulation of heme-biosynthesis-related genes plays an important role in eggshell pigment production in Japanese quails.
Article
Genetics & Heredity
Yuuki Kobayashi, Tomoko F. Shibata, Hideki Hirakawa, Tomoaki Nishiyama, Akiyoshi Yamada, Mitsuyasu Hasebe, Shuji Shigenobu, Masayoshi Kawaguchi
Summary: Mycorrhizae are an important symbiotic relationship between plants and fungi, with ectomycorrhizae being the most common in boreal forest ecosystems. The genomic changes associated with the transition to the ectomycorrhizal lifestyle are not well understood.
Article
Plant Sciences
Naoyuki Sotta, Satoshi Niikura, Takehiro Kamiya, Toru Fujiwara
Summary: In this study, a culture method was established to observe brown heart in Japanese radish under controlled conditions. It was found that boron deficiency alone, without heat and drought stresses, can cause internal browning. Additionally, differences in susceptibility to this symptom were observed among different cultivars, suggesting the applicability of this culture method for inter-cultivar variation studies such as QTL mapping.
SOIL SCIENCE AND PLANT NUTRITION
(2023)
Article
Biochemistry & Molecular Biology
Ryohei H. Suzuki, Takumi Hanada, Yoshinobu Hayashi, Shuji Shigenobu, Kiyoto Maekawa, Masaru K. Hojo
Summary: Termites have an intricate social system with cooperation and division of labor. The perception of chemical signals in this social system is still not well understood. This study identified chemosensory genes involved in signal transduction in termites and found that the expression levels of certain non-receptor genes are affected by both individual and collective behaviors among termite colony members.
INSECT MOLECULAR BIOLOGY
(2023)
Article
Plant Sciences
Qi Wu, Zhihang Feng, Hironaka Tsukagoshi, Miaoyan Yang, Yan Ao, Toru Fujiwara, Takehiro Kamiya
Summary: Low potassium condition affects the permeability of the Casparian strip, lignin deposition, and MYB36 mRNA accumulation. Nitric oxide mediates the root endodermal cell lignification response to low-potassium conditions through the MYB36-associated lignin-polymerizing pathway, maintaining nutrient homeostasis and correct apoplastic barrier formation. This study reveals the novel physiological roles of the Casparian strip under low nutrient conditions.
Article
Plant Sciences
Chihiro Miura, Yuki Furui, Tatsuki Yamamoto, Yuri Kanno, Masaya Honjo, Katsushi Yamaguchi, Kenji Suetsugu, Takahiro Yagame, Mitsunori Seo, Shuji Shigenobu, Masahide Yamato, Hironori Kaminaka
Summary: Orchids autoactivate the mycorrhizal symbiosis pathway through gibberellin inactivation to accept the fungal partner during seed germination.