Article
Peripheral Vascular Disease
Xi Yuan, Xiyao Liu, Fangyu Zhu, Biao Huang, Li Lin, Jiayu Huang, Li Wen, Mark D. Kilby, Philip N. Baker, Yong Fu, Weiwei Wu, Hongbo Qi, Jing Tang, Chao Tong
Summary: This study investigates the relationship among HtrA4, endoplasmic reticulum stress (ERS), and trophoblast syncytialization in the development of early-onset preeclampsia (EO-PE). The findings suggest that ERS enables syncytialization of placental development by upregulating HtrA4, but excessive HtrA4 expression and preexisting ERS impair syncytialization and cause EO-PE.
JOURNAL OF HYPERTENSION
(2023)
Article
Developmental Biology
P. Vishnyakova, A. Poltavets, M. Nikitina, K. Muminova, A. Potapova, V. Vtorushina, N. Loginova, K. Midiber, L. Mikhaleva, A. Lokhonina, Z. Khodzhaeva, A. Pyregov, A. Elchaninov, T. Fatkhudinov, G. Sukhikh
Summary: Our study aimed to characterize the phenotype of decidual macrophages and plasma profiles in patients with late- and early-onset preeclampsia compared to controls. We observed significant increases in inflammation-associated CD86+ and CX3CR1+ cells in early-onset preeclampsia, while the portion of CD163+ cells was higher in all studied groups. There were also significant alterations in gene expression and protein levels in the decidual cells of early-onset preeclampsia samples, as well as elevation of pro- and anti-inflammatory cytokines in the plasma of preeclampsia groups.
Article
Endocrinology & Metabolism
Hiroko Oda, Takeshi Nagamatsu, Danny J. Schust, Horacio Cabral, Takuya Miyazaki, Takayuki Iriyama, Kei Kawana, Yutaka Osuga, Tomoyuki Fujii
Summary: The novel anticoagulant rTM has shown promising therapeutic potential for treating preeclampsia by inhibiting HMGB1-mediated pathways and ameliorating factors that induce systemic endothelial dysfunction, as confirmed in in vivo and in vitro experiments.
Article
Immunology
Zi Lv, Ding-Yi Lv, Jia-Yu Meng, Xiao-Yan Sha, Xue-Ya Qian, Yun-Shan Chen, Xiu-Yu Pan, Guang-Yuan Yu, Hui-Shu Liu
Summary: This study aimed to investigate the potential correlation between trophoblastic mitochondrial DNA (mtDNA) release and endothelium damage in preeclampsia (PE). The results suggest that trophoblastic mtDNA damages the endothelial cells via NLRP3 activation, leading to inflammation and impaired vascular relaxation. This finding is of great significance for understanding the pathogenesis of PE and identifying new therapeutic approaches.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2023)
Article
Endocrinology & Metabolism
Lydia Baird, Ping Cannon, Manju Kandel, Tuong-Vi Nguyen, Anna Nguyen, Georgia Wong, Ciara Murphy, Fiona C. Brownfoot, Elif Kadife, Natalie J. Hannan, Stephen Tong, Lucy A. Bartho, Tu'uhevaha J. Kaitu'u-Lino
Summary: This study found that the expression of the retrotransposon PEG10 is reduced in the placentas of patients with early-onset preeclampsia. In vitro studies suggest that hypoxia and inflammation may contribute to the downregulation of PEG10. Reduced PEG10 alters the canonical TGF-beta signaling and may be involved in trophoblast dysfunction.
REPRODUCTIVE BIOLOGY AND ENDOCRINOLOGY
(2023)
Article
Multidisciplinary Sciences
Mary Mansilla, Yao Wang, Rebecca Lim, Kirsten Palmer, Guiying Nie
Summary: HtrA4 is crucial for syncytialization in the human placenta, as its expression correlates significantly with the secretion of beta-hCG, suggesting an essential role in this process.
SCIENTIFIC REPORTS
(2021)
Review
Cell Biology
Priscila Rezeck Nunes, Sarah Viana Mattioli, Valeria Cristina Sandrim
Summary: Preeclampsia is a specific syndrome of human pregnancy and a leading cause of maternal death. Persistent inflammation in the endothelium activates various signaling pathways, including NLRP3, exacerbating oxidative stress and endothelial dysfunction. Inhibition of NLRP3 activation may offer potential therapeutic solutions for this condition in the future.
Article
Biochemistry & Molecular Biology
Maja Gajic, Bianca Schroeder-Heurich, Monika Horvat Mercnik, Mila Cervar-Zivkovic, Christian Wadsack, Frauke von Versen-Hoeynck, Karoline Mayer-Pickel
Summary: This study isolated arterial feto-placental endothelial cells from control and preeclamptic placentas to investigate the potential protective effects of hydroxychloroquine (HCQ) in an in vitro model of endothelial activation. The results showed that HCQ did not adversely affect wound healing and endothelial barrier integrity, and it had a slight regulatory effect on IL-8 and VCAM-1 gene expression in the presence of inflammation. These findings support the safety of HCQ and highlight the need for clinical trials to evaluate its therapeutic effects in preeclampsia.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Medicine, Research & Experimental
Zhonglu Ren, Yunfei Gao, Yue Gao, Guanmei Liang, Qian Chen, Sijia Jiang, Xiaoxue Yang, Cuixia Fan, Haizhen Wang, Jing Wang, Yi-Wu Shi, Chaoqun Xiao, Mei Zhong, Xinping Yang
Summary: The study found that early-onset severe preeclampsia and late-onset severe preeclampsia have different molecular mechanisms, with the former associated with metabolism-related pathways and the latter with immune-related pathways. Some key transcription factors may be driving the widespread gene dysregulation in both early-onset and late-onset patients.
Article
Biochemistry & Molecular Biology
Jakub Kornacki, Pawel Gutaj, Anastasia Kalantarova, Rafal Sibiak, Maurycy Jankowski, Ewa Wender-Ozegowska
Summary: The endothelium, crucial in various physiological functions, undergoes changes during pregnancy that are associated with gestational complications. Research is needed to elucidate mechanisms, find specific clinical markers, and incorporate them into predictive models for improved prognostic value. Further studies are required to fine-tune clinical markers and explore therapeutic techniques for preventing or reversing endothelial damage.
Article
Biochemistry & Molecular Biology
Yue Bian, Jiapo Li, Hongfei Shen, Yuanyuan Li, Yue Hou, Ling Huang, Guiyu Song, Chong Qiao
Summary: This study found that the level of N6-methyladenosine (m6A) in placental tissue of early-onset preeclampsia (ePE) patients was reduced. Wilms' tumor 1-associating protein (WTAP), a regulator of m6A, was found to regulate trophoblast cell migration and invasion. The study also revealed the interaction between WTAP and the high mobility group nucleosomal binding domain 3 (HMGN3) gene, and the effect of m6A on the stability of HMGN3 mRNA. WTAP enhanced the stability of HMGN3 mRNA by binding to its m6A site, and HMGN3 was recognized and regulated by insulin-like growth factor 2 mRNA-binding protein 1 (IGF2BP1). The results suggest that m6A modification plays an important role in the pathogenesis of ePE.
Article
Biochemistry & Molecular Biology
Alvaro Santana-Garrido, Claudia Reyes-Goya, Pablo Espinosa-Martin, Luis Sobrevia, Luis M. Beltran, Carmen M. Vazquez, Alfonso Mate
Summary: This study investigates the role of the NADPH oxidase system in a rodent model of preeclampsia. The results suggest that NADPH oxidase mediates oxidative stress and inflammation in the placenta and kidney, providing important insights into the pathogenesis and potential treatment of preeclampsia.
Article
Physiology
Shunping Gui, Shengping Zhou, Min Liu, Yanping Zhang, Linbo Gao, Tao Wang, Rong Zhou
Summary: In patients with severe preeclampsia (sPE), the upregulation of soluble Axl (sAxl) in maternal plasma is positively correlated with elevated levels of sFlt-1 and negatively correlated with placental NO synthase (eNOS). Elevated sAxl levels suppress proliferation and tube formation of endothelial cells, and promote cytotoxicity. The study also demonstrates that sAxl plays a role in systemic vascular injury through the regulation of key molecules of angiogenesis, contributing to the development of sPE.
FRONTIERS IN PHYSIOLOGY
(2021)
Article
Obstetrics & Gynecology
Sibel Ozler, Aysegul Kebapcilar, Ebru Marzioglu Ozdemir, Muhammed Mert, Mehmet Nurullah Arikan, Cetin Celik
Summary: This study aimed to investigate the serum MicroRNA (miRNA) levels in preeclampsia (PE) and determine their predictive value in early onset preeclampsia (EOPE) and adverse perinatal outcomes. The study found that serum levels of miR-17, miR-20a, miR-20b, miR126, and miR-210 were significantly higher in PE patients compared to the control group. Increased levels of miR-17, miR-20a, and miR-20b were independently associated with PE, and increased miR-17 and miR-126 levels were negatively correlated with EOPE in PE patients, while increased miR-210 levels were positively correlated with EOPE in PE patients.
REPRODUCTIVE SCIENCES
(2023)
Article
Obstetrics & Gynecology
Anna E. Stanhewicz, Gabrielle A. Dillon, Corinna Serviente, Lacy M. Alexander
Summary: The study suggests that acute systemic inhibition of vascular inflammation can improve endothelial function in women with a history of preeclampsia, but not in women with a history of uncomplicated pregnancy.
PREGNANCY HYPERTENSION-AN INTERNATIONAL JOURNAL OF WOMENS CARDIOVASCULAR HEALTH
(2022)
Article
Multidisciplinary Sciences
Yao Wang, Rebecca Lim, Guiying Nie
SCIENTIFIC REPORTS
(2019)
Article
Obstetrics & Gynecology
Sonia Soo Yee Teoh, Yao Wang, Ying Li, Shalem Yiner-Lee Leemaqz, Gus A. Dekker, Claire T. Roberts, Guiying Nie
FETAL DIAGNOSIS AND THERAPY
(2019)
Article
Obstetrics & Gynecology
Daniel Lorber Rolnik, Yao Wang, Jonathan Hyett, Fabricio da Silva Costa, Guiying Nie
JOURNAL OF PERINATOLOGY
(2019)
Article
Developmental Biology
Yao Wang, Rebecca Lim, Guiying Nie
Article
Acoustics
D. Wertaschnigg, D. L. Rolnik, G. Nie, S. S. Y. Teoh, A. Syngelaki, F. da Silva Costa, K. H. Nicolaides
ULTRASOUND IN OBSTETRICS & GYNECOLOGY
(2020)
Article
Multidisciplinary Sciences
Xing-Xing Gao, Meng-Ying Ye, Yan Liu, Jin-Yan Li, Li Li, Wei Chen, Xue Lu, Guiying Nie, Yuan-Hua Chen
SCIENTIFIC REPORTS
(2020)
Article
Developmental Biology
Sasha Skinner, Daniel L. Rolnik, Yao Wang, Guiying Nie, Argyro Syngelaki, Kypros H. Nicolaides, Fabricio da Silva Costa
Summary: There was no significant difference in serum HtrA1 levels in women who developed PE compared to controls. Women who later developed PE had higher maternal weight and a higher frequency of chronic hypertension or a history of PE in a previous pregnancy.
Article
Obstetrics & Gynecology
Sarah G. Paule, Sophea Heng, Nirukshi Samarajeewa, Ying Li, Mary Mansilla, Andrew Webb, Thomas Nebl, Steven L. Young, Bruce A. Lessey, M. Louise Hull, Maxine Scelwyn, Rebecca Lim, Beverley Vollenhoven, Luk J. Rombauts, Guiying Nie
Summary: This study identified PODX as a key negative regulator of endometrial receptivity, with its specific downregulation in the mid-secretory phase likely mediated by progesterone. Overexpression of PODX inhibited human embryo attachment and invasion in functional models, highlighting its role as a negative regulator of epithelial receptivity for implantation.
HUMAN REPRODUCTION
(2021)
Article
Obstetrics & Gynecology
Sophea Heng, Nirukshi Samarajeewa, Asma Aberkane, Wafaa Essahib, Hilde Van de Velde, Maxine Scelwyn, M. Louise Hull, Beverley Vollenhoven, Luk J. Rombauts, Guiying Nie
Summary: This study found that PCX inhibits embryo implantation in vitro and in IVF patients. High levels of PCX significantly inhibited blastocyst attachment and penetration. The presence of PCX in the luminal epithelium was negatively associated with the success rate of clinical pregnancy.
FERTILITY AND STERILITY
(2021)
Article
Multidisciplinary Sciences
Mary Mansilla, Yao Wang, Rebecca Lim, Kirsten Palmer, Guiying Nie
Summary: HtrA4 is crucial for syncytialization in the human placenta, as its expression correlates significantly with the secretion of beta-hCG, suggesting an essential role in this process.
SCIENTIFIC REPORTS
(2021)
Review
Oncology
Ngoc Le Tran, Yao Wang, Guiying Nie
Summary: Malignancies derived from epithelial cells make up over 90% of all human cancers, with progression often associated with metastasis, immune evasion, and chemotherapy resistance. Podocalyxin, as a potential diagnostic and prognostic biomarker, has shown to be overexpressed in many cancers and linked to poor prognosis, aggressive tumor progression, and chemoresistance. This review summarizes the current knowledge of Podocalyxin in normal tissues and epithelial cancers, emphasizing its potential utility in cancer management.
Review
Biochemistry & Molecular Biology
Yao Wang, Guiying Nie
Summary: The HtrA family of proteins, consisting of four homologs, play important physiological roles in different tissues in humans. HtrA4 stands out for its limited expression and function in the placenta. Studying these proteins can enhance our understanding of critical cellular processes for human health.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Sophea Heng, Nirukshi Samarajeewa, Yao Wang, Sarah G. Paule, James Breen, Guiying Nie
Summary: PCX is a key negative regulator of human endometrial receptivity, suppressing genes controlling cell adhesion and communication while increasing expression of proteins governing epithelial barrier functions. It also suppresses receptivity-supporting factors and signaling pathways, and stimulates anti-implantation genes. Overall, PCX promotes an anti-adhesive and impermeable epithelium while negatively controlling endometrial receptivity for implantation.
SCIENTIFIC REPORTS
(2021)
Review
Biochemistry & Molecular Biology
Manizha Shekibi, Sophea Heng, Guiying Nie
Summary: The development of endometrial receptivity is crucial for successful embryo implantation and pregnancy initiation. Recent studies have focused on the role of microRNAs (miRNAs) in the regulation of endometrial receptivity. This review provides an up-to-date knowledge of the miRNAs identified in humans and mice that may play a functional role in the establishment of receptivity. The findings suggest that miRNAs could be potential biomarkers and therapeutic targets for improving endometrial receptivity in human fertility treatment.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Developmental Biology
Manizha Shekibi, Sophea Heng, Yao Wang, Nirukshi Samarajeewa, Luk Rombauts, Guiying Nie
Summary: This study provides evidence that progesterone down-regulates PODXL through miR-145 and miR-199, enhancing human endometrial epithelial receptivity to embryo implantation.
MOLECULAR HUMAN REPRODUCTION
(2022)