Article
Biotechnology & Applied Microbiology
Rui Zheng, Daiying Song, Yangfan Ding, Binbin Sun, Changrui Lu, Xiumei Mo, Hui Xu, Yu Liu, Jinglei Wu
Summary: Injury to the meniscus is a common challenge in the clinic. The study compares three commonly used cell sources (BMSC, ADSC, and chondrocytes) for engineering meniscus tissue. Chondrocytes show superior characteristics for constructing chondrogenic tissues, while stem cells are feasible for forming fibroblastic tissues. Combining chondrocytes and stem cells may be a potential solution for meniscus repair and regeneration.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Orthopedics
Alexander R. A. Szojka, Yan Liang, Rita de Cassia Marqueti, Colleen N. Moore, Esra J. N. Erkut, Melanie Kunze, Aillette Mulet-Sierra, Nadr M. Jomha, Adetola B. Adesida
Summary: This study investigated the time course of fibrocartilage formation by human meniscus fibrochondrocytes under various static conditions. Mechanical properties of the fibrocartilage were positively correlated with glycosaminoglycan content and accumulation of hyaline cartilage-like matrix components, and were lower in hypoxia but increased continually in normoxia. The most mechanically competent tissues reached a fraction of the native meniscus instantaneous compression modulus and exhibited a hypertrophic matrix-forming phenotype, which was consistently suppressed under hypoxia.
JOURNAL OF ORTHOPAEDIC RESEARCH
(2022)
Review
Biotechnology & Applied Microbiology
Guocheng Ding, Jianing Du, Xiaoqing Hu, Yingfang Ao
Summary: This review aims to summarize and compare the application of mesenchymal stem cells from different sources for meniscal repair and regeneration. Although mesenchymal stem cells have shown specific advantages in meniscus repair, their cartilage-forming properties are yet to be systematically compared across different sources.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Orthopedics
Jisoo Lee, Seoyoung Jang, JunPyo Kwon, Tong In Oh, EunAh Lee
Summary: The research aimed to compare the fibrocartilage construction capability of different cell sources in the presence of mechanical stimuli. Results showed that MCs exhibited GAG-rich matrix formation during default in vitro chondrogenesis, while SvMSCs had superior cell growth characteristics but higher senescence tendency. Static tension negatively impacted GAG production in MCs but did not significantly affect their tissue growth, whereas SvMSCs showed inferior chondrogenic differentiation and tissue growth under static tension.
Article
Cell & Tissue Engineering
Seoyoung Jang, Jisoo Lee, Jin Gil Jeong, Tong In Oh, EunAh Lee
Summary: To reconstruct functional fibrocartilage, it is crucial to replicate the unique mechanical properties exhibited by natural fibrocartilage, which are caused by the distinct histological features of fibrocartilage. Our study reveals that tensile stimulation induces an antichondrogenic effect on scaffold-free tissues constructed with meniscal chondrocytes (MCs), but this effect can be ameliorated by blocking nuclear translocation of Yes-associated protein (YAP). We also found that by optimizing the dose and timing of mechanical training, uniaxial alignment of type I collagen can be achieved in the cartilaginous matrix.
TISSUE ENGINEERING PART A
(2023)
Article
Biochemistry & Molecular Biology
Meaghan E. Harley-Troxell, Madhu Dhar
Summary: Neural injuries disrupt normal nervous system function, but neurospheres have the potential to advance regenerative medicine and neural tissue engineering. By optimizing conditions, we successfully developed primary neural cell-derived neurospheres using a stress-free, 3D bioreactor, providing a necessary baseline for future studies and therapeutic use.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Orthopedics
Nobuhiro Okuno, Shuhei Otsuki, Jo Aoyama, Kosuke Nakagawa, Tomohiko Murakami, Kuniaki Ikeda, Yoshinobu Hirose, Hitoshi Wakama, Tomohiro Okayoshi, Yoshinori Okamoto, Yoshiaki, Masashi Neo
Summary: The study demonstrated that the peptide hydrogel scaffold KI24RGDS can promote repair and regeneration of meniscus in a rabbit model, showing significant healing effects. The hydrogel is highly biocompatible and biodegradable, mimicking the three-dimensional structure of the extracellular matrix and promoting cell adhesion and proliferation.
JOURNAL OF ORTHOPAEDIC RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Zahra Abpeikar, Moosa Javdani, Akram Alizadeh, Pegah Khosravian, Lobat Tayebi, Shiva Asadpour
Summary: The lack of vascularization in the white-red and white zone of the meniscus hinders its self-healing capacity and may accelerate osteoarthritis. This study developed a hybrid construct as a meniscus tissue engineering scaffold with good regenerative potential. The scaffold showed promising results in both in vitro and in vivo experiments.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Engineering, Biomedical
Zhou Jian, Tian Zhuang, Tian Qinyu, Peng Liqing, Li Kun, Luo Xujiang, Wang Diaodiao, Yang Zhen, Jiang Shuangpeng, Sui Xiang, Huang Jingxiang, Liu Shuyun, Hao Libo, Tang Peifu, Yao Qi, Guo Quany
Summary: By optimizing the preparation of meniscus-derived bioink and utilizing a custom 3D bioprinting system, a biomimetic meniscal scaffold with optimal features in terms of morphology and components was successfully created. Comprehensive evaluations were conducted on cell viability, mechanics, biodegradation, and tissue formation to ensure the scaffold's feasibility and functionality for application in tissue engineering.
BIOACTIVE MATERIALS
(2021)
Article
Biotechnology & Applied Microbiology
Xingyu Deng, Xiabin Chen, Fang Geng, Xin Tang, Zhenzhen Li, Jie Zhang, Yikai Wang, Fangqian Wang, Na Zheng, Peng Wang, Xiaohua Yu, Shurong Hou, Wei Zhang
Summary: The study utilized 3D printing technology with cryo-printing to create polyurethane scaffolds that mimic native meniscus, improving bioactivity for hMSCs culture. Surface modification with fibronectin was found to enhance stem cell functions and favor long-term chondrogenesis. The scaffolds successfully facilitated hMSCs chondrogenic differentiation and demonstrated good in vivo biocompatibility, highlighting their potential for meniscus regeneration.
JOURNAL OF NANOBIOTECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Farshad Amiri, Melika Babaei, Nima Jamshidi, Maria Agheb, Mohammad Rafienia, Mohammad Kazemi
Summary: The study investigated the structural and biological properties of 3D-printed polyurethane scaffolds coated with gellan gum, hyaluronic acid, and glucosamine. The surface coating improved cell behavior, increased expression levels of collagen II and aggrecan genes, and enhanced the scaffold's mechanical and degradation properties, making it a promising candidate for meniscus tissue engineering.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Review
Chemistry, Medicinal
Gollahalli Shivashankar Prajwal, Naveen Jeyaraman, Krishna Kanth, Madhan Jeyaraman, Sathish Muthu, Sree Naga Sowndary Rajendran, Ramya Lakshmi Rajendran, Manish Khanna, Eun Jung Oh, Kang Young Choi, Ho Yun Chung, Byeong-Cheol Ahn, Prakash Gangadaran
Summary: Tissue engineering and regenerative medicine provide a new approach for treating musculoskeletal diseases, utilizing MSCs and growth factors to improve the regenerative environment. However, the selection of MSCs source and the long-term and short-term effects of MSCs treatment remain uncertain.
Article
Biotechnology & Applied Microbiology
Hao Li, Zhiyao Liao, Zhen Yang, Cangjian Gao, Liwei Fu, Pinxue Li, Tianyuan Zhao, Fuyang Cao, Wei Chen, Zhiguo Yuan, Xiang Sui, Shuyun Liu, Quanyi Guo
Summary: This study presents a composite scaffold for meniscus tissue engineering, consisting of a 3D-printed PCL scaffold, injected MECM, and KGN-loaded PLGA microspheres. The combination showed enhanced hydrophilicity, bioactivity, cell adhesion, proliferation, and chondrogenic differentiation. The sustained release of KGN from the scaffold contributed to improved cell affinity and chondrogenic activity, indicating promising application prospects in MTE.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Engineering, Biomedical
Ming-Ze Du, Yun Dou, Li-Ya Ai, Tong Su, Zhen Zhang, You-Rong Chen, Dong Jiang
Summary: This article systematically summarizes the heterogeneity of menisci and the application of 3D printing technology in tissue-engineered menisci. The manufacturing techniques, biomaterial combinations, surface functionalization, growth factors, and bioreactors related to 3D-printed strategies are introduced, and a promising direction for future research is proposed.
INTERNATIONAL JOURNAL OF BIOPRINTING
(2023)
Review
Biochemistry & Molecular Biology
Daniela Trindade, Rachel Cordeiro, Henrique Cardoso Jose, David Faustino Angelo, Nuno Alves, Carla Moura
Summary: The temporomandibular joint (TMJ) is crucial for the masticatory system, and pathologies affecting it are common and impactful. Finding a permanent solution through tissue engineering to rebuild the disc is imperative for improving quality of life for those with TMJ problems.
Article
Chemistry, Physical
Jennifer K. Lee, Le W. Huwe, Nikolaos Paschos, Ashkan Aryaei, Courtney A. Gegg, Jerry C. Hu, Kyriacos A. Athanasiou
Letter
Orthopedics
Dimitrios Giotis, Nikolaos K. Paschos, George Mantellos
FOOT AND ANKLE SURGERY
(2017)
Review
Engineering, Biomedical
Siobhan A. O'Leary, Nikolaos K. Paschos, Jarrett M. Link, Eric O. Klineberg, Jerry C. Hu, Kyriacos A. Athanasiou
ANNUAL REVIEW OF BIOMEDICAL ENGINEERING, VOL 20
(2018)
Editorial Material
Orthopedics
Nikolaos K. Paschos
ARTHROSCOPY-THE JOURNAL OF ARTHROSCOPIC AND RELATED SURGERY
(2018)
Article
Multidisciplinary Sciences
Heenam Kwon, Anne K. Haudenschild, Wendy E. Brown, Natalia Vapniarsky, Nikolaos K. Paschos, Boaz Arzi, Jerry C. Hu, Kyriacos A. Athanasiou
Editorial Material
Orthopedics
Nikolaos K. Paschos
ARTHROSCOPY-THE JOURNAL OF ARTHROSCOPIC AND RELATED SURGERY
(2018)
Article
Orthopedics
Robert C. Spang, Frances A. Tepolt, Nikolaos K. Paschos, Lauren H. Redler, Eric A. Davis, Mininder S. Kocher
JOURNAL OF PEDIATRIC ORTHOPAEDICS
(2019)
Article
Sport Sciences
Justin Peterson, Nikolaos K. Paschos, John C. Richmond
OPERATIVE TECHNIQUES IN SPORTS MEDICINE
(2018)
Editorial Material
Orthopedics
Nikolaos K. Paschos
ARTHROSCOPY-THE JOURNAL OF ARTHROSCOPIC AND RELATED SURGERY
(2019)
Review
Rheumatology
Heenam Kwon, Wendy E. Brown, Cassandra A. Lee, Dean Wang, Nikolaos Paschos, Jerry C. Hu, Kyriacos A. Athanasiou
NATURE REVIEWS RHEUMATOLOGY
(2019)
Editorial Material
Orthopedics
Nikolaos K. Paschos
ARTHROSCOPY-THE JOURNAL OF ARTHROSCOPIC AND RELATED SURGERY
(2019)
Editorial Material
Orthopedics
Nikolaos K. Paschos, Jefferson C. Brand, Michael J. Rossi, James Lubowitz
ARTHROSCOPY-THE JOURNAL OF ARTHROSCOPIC AND RELATED SURGERY
(2019)
Editorial Material
Orthopedics
Nikolaos K. Paschos
ARTHROSCOPY-THE JOURNAL OF ARTHROSCOPIC AND RELATED SURGERY
(2020)
Article
Orthopedics
Brian W. Yang, Elizabeth S. Liotta, Nikolaos Paschos
CURRENT REVIEWS IN MUSCULOSKELETAL MEDICINE
(2019)
Article
Engineering, Biomedical
E. Y. Salinas, A. Aryaei, N. Paschos, E. Berson, H. Kwon, J. C. Hu, K. A. Athanasiou