Article
Biochemistry & Molecular Biology
Yung-Fu Wu, Chih-Yang Wang, Wan-Chun Tang, Yu-Cheng Lee, Hoang Dang Khoa Ta, Li-Chia Lin, Syu-Ruei Pan, Yi-Chun Ni, Gangga Anuraga, Kuen-Haur Lee
Summary: Colorectal cancer is a heterogeneous disease with genetic and epigenetic changes, and molecular assessment along with biomarker use for prognosis is gaining attention. This study identifies high mRNA levels of 10 upregulated genes in CRC cells and datasets, showing correlation with worse prognosis in CRC patients. CTNNB1 is found to be a key factor in the interaction with upregulated CRC-associated genes, particularly regulating NKD2, FOXQ1, and CEMIP, which when inhibited, can suppress CRC cell growth, providing efficient biomarkers for prognosis and a new molecular interaction network for CRC.
Article
Biochemistry & Molecular Biology
Satadeepa Kal, Shrabastee Chakraborty, Subhajit Karmakar, Mrinal K. Ghosh
Summary: This study elucidates a novel mechanism of transcriptional activation of the CHIP gene mediated by Wnt/beta-catenin and p68, leading to enhanced proliferation of colorectal carcinoma cells. It demonstrates the binding of beta-catenin and p68 to the CHIP promoter and regulation of its transcription. This is the first report to uncover the mechanistic details of CHIP gene expression regulation.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Yuri Lee, Hai-long Piao, Jongchan Kim
Summary: The Wnt signaling pathway is crucial for regulating various cellular processes, and dysregulation of this pathway has been linked to human diseases, including cancer. In this study, researchers aimed to identify the deubiquitinases (DUBs) that regulate the pathway through the essential component LEF1. They discovered that OTUD7B interacts with LEF1 and activates Wnt signaling. Furthermore, OTUD7B promotes the nuclear localization of LEF1, leading to increased interaction with beta-catenin. This study suggests that OTUD7B may serve as a potential therapeutic target in diseases where Wnt signaling is dysregulated, such as cancer.
Article
Oncology
Yang Zhou, Jiang Xu, Haichang Luo, Xiangjing Meng, Ming Chen, Di Zhu
Summary: Abnormal activation of the Wnt/beta-catenin signaling pathway is closely related to tumorigenesis and immune surveillance, leading to increased resistance to immunotherapy.
Article
Environmental Sciences
Yuzhuo Chen, Zhirong Yang, Xingqiang He, Wanglong Zhu, Yujun Wang, Jiaofeng Li, Zhengyu Han, Jie Wen, Wei Liu, Yuhan Yang, Kun Zhang
Summary: Proanthocyanidins (PC) have powerful antioxidant and anti-aging effects, and play an important role in tumor cell growth, drug resistance, recurrence, and metastasis. Recent studies have found that PC exhibits inhibitory effects on colorectal cancer stem cells (CSCs) through the Wnt/β-catenin pathway.
ENVIRONMENTAL TOXICOLOGY
(2023)
Article
Agriculture, Multidisciplinary
Hui Zhao, Shun Tang, Qiu Tao, Tianqi Ming, Jiarong Lei, Yuanjing Liang, Yuhui Peng, Minmin Wang, Maolun Liu, Han Yang, Shan Ren, Haibo Xu
Summary: Overwhelming evidence supports the role of the Wnt/beta-catenin signaling pathway in colorectal cancer (CRC) pathogenesis. Ursolic acid (UA), a pentacyclic triterpenoid found in various fruits and plants, has shown potent anticancer activity against CRC, although its mechanism of action is not fully understood. This study aimed to investigate the effects of UA on CRC and its impact on the Wnt/beta-catenin signaling pathway. The study demonstrated that UA significantly inhibited proliferation, migration, and clonality, induced apoptosis, and arrested the cell cycle in CRC cells. Moreover, UA suppressed tumor growth, improved pathological features, induced apoptosis, and arrested the cell cycle in CRC tissue, by diminishing the Wnt/beta-catenin signaling pathway. These findings suggest that UA has the potential to inhibit the malignant phenotype of CRC by attenuating the Wnt/beta-catenin signaling axis.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Oncology
Weitao Zhang, Lu Kong, Hongbin Zhu, Decong Sun, Quanli Han, Bin Yan, Zhi Cui, Weiwei Zhang, Shurong Zhang, Xindan Kang, Guanghai Dai, Niansong Qian, Wenji Yan
Summary: RAI2 inhibits Wnt signaling by interacting with or down-regulating CtBP2, resulting in repression of stem cell-like properties and increased chemosensitivity of CRC cells.
FRONTIERS IN ONCOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Yanmei Zhang, Zixuan Wang, Qifan Hu, Hao Luo, Bingchuan Lu, Yunhe Gao, Zhi Qiao, Yongsen Zhou, Yongcong Fang, Jin Gu, Ting Zhang, Zhuo Xiong
Summary: This study identifies a new biomaterial and method for inducing and enriching colorectal cancer stem cells (CSCs) using three-dimensional bioprinting. The use of GelMA-nanoclay hydrogels promotes the formation of spheroids expressing stemness markers and enhances the self-renewal, differentiation, and tumorigenic capacity of cancer cells in vitro and in vivo. The study also demonstrates that spheroids from GelMA-nanoclay hydrogels have increased stemness and sensitivity to anti-CSC compounds compared to traditional methods. This research is significant for facilitating effective CSC-targeting drug screening.
Article
Biochemistry & Molecular Biology
Mingliang He, Xiaoyang Lv, Xiukai Cao, Zehu Yuan, Tesfaye Getachew, Yutao Li, Shanhe Wang, Wei Sun
Summary: SRY-box transcription factor 18 (SOX18) is expressed in dermal papilla cells (DPCs) of sheep hair follicles and plays a crucial role in promoting DPC proliferation through activating the Wnt/β-Catenin signaling pathway. Inhibition of the Wnt/β-Catenin signaling pathway can be rescued by SOX18, suggesting the essential role of SOX18 in governing DPC proliferation and hair growth.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Biochemistry & Molecular Biology
Ellen Youngsoo Rim, Hans Clevers, Roel Nusse
Summary: The Wnt pathway plays a central role in developmental and disease-related processes, regulating the generation and spatial arrangement of different cell types. Through regulation of cell fate, mitotic activity, and cell polarity, Wnt signaling coordinates development and tissue homeostasis, and its dysregulation is implicated in various disorders. This review summarizes the advances in our understanding of this pathway, from Wnt protein production to signal transduction in receiving cells. The evolutionary history of this pathway, as well as its regulators, and the remaining gaps in knowledge are discussed, highlighting directions for future research.
ANNUAL REVIEW OF BIOCHEMISTRY
(2022)
Article
Medicine, Research & Experimental
Liang Zhang, Chuan-fu Ren, Zhi Yang, Long-bo Gong, Chao Wang, Min Feng, Wen-xian Guan
Summary: FOXS1 plays a role in promoting cell proliferation, migration, invasion, and metastasis in colorectal cancer through the Wnt/beta-catenin signaling pathway, making it a potential target for CRC treatment.
JOURNAL OF TRANSLATIONAL MEDICINE
(2022)
Article
Cell Biology
Xiaolong Tang, Yahang Liang, Guorui Sun, Qingsi He, Hui Qu, Peng Gao
Summary: UBQLN4, a key member of the ubiquitin-like protein family, is upregulated in CRC tissues and associated with tumor size, stage, and metastasis. Its overexpression promotes CRC cell proliferation, migration, and invasion through activation of the Wnt/beta-catenin pathway and C/EBP beta transcriptional activation. Targeting UBQLN4 may hold promise for the diagnosis and treatment of CRC patients.
CELL DEATH DISCOVERY
(2021)
Article
Cell & Tissue Engineering
Xuan Wang, Tianwei He, Lei He, Bu Yang, Zhongyu Liu, Mao Pang, Peigen Xie, Liangming Zhang, Limin Rong
Summary: The study demonstrates that melatonin enhances the hypertrophic differentiation of MSC-derived chondrocytes through the Wnt signaling pathway, increasing chondrocyte cell volume and type X collagen expression while decreasing type II collagen expression.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Oncology
Yujing Liu, Chunyan Wang, Peiqiu Cheng, Shengan Zhang, Wenjun Zhou, Yangxian Xu, Hanchen Xu, Guang Ji
Summary: This study found that low FHL1 expression is associated with poor patient survival in colorectal cancer. Experimental results indicate that FHL1 acts as a tumor suppressor in CRC, primarily by inhibiting cell proliferation through regulating the Wnt/beta-catenin signaling pathway.
Article
Oncology
Wulin Shan, Chunyang Dai, Huanhuan Zhang, Dan Han, Qiyi Yi, Bairong Xia
Summary: This study found that downregulation of ACY1 could enhance the radiosensitivity of cetuximab-resistant CRC by inactivating the Wnt/β-catenin signaling pathway. These findings suggest that ACY1 may serve as a potential radiotherapy target for cetuximab-resistant CRC.
