Journal
FOOD SCIENCE AND HUMAN WELLNESS
Volume 12, Issue 5, Pages 1762-1771Publisher
KEAI PUBLISHING LTD
DOI: 10.1016/j.fshw.2023.02.027
Keywords
Potato protease inhibitors; Antioxidant activity; Cytoprotection; Anticancer activity
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Potato protease inhibitors (PPIs), derived from potato protein isolate, have excellent safety, nutritional value, and a promising market potential. Cell-based assays have shown that PPIs possess antioxidative and anticancer properties. PPIs at a concentration of 5 mg/mL demonstrated a peroxyl radical scavenging value of (2,119 +/- 204) mg VCE/100 g, and cellular antioxidant activity values of (45.83 +/- 3.5) (no PBS wash) and (33.25 +/- 4.4) mu mol QE/100 g (PBS wash). Pre-treatment with PPIs prevented oxidative damage, inhibited morphological changes, and exhibited selective anti-proliferative effects on GIST882 cells (IC50 = (10.53 +/- 3.87) mg/mL). PPIs also showcased potent inhibition of cancer cell growth, migration, and invasion. These findings support the potential usage of PPIs as functional foods for preventing oxidative damage and cancer.
Potato protease inhibitors (PPIs), as the main component of potato protein isolate, have good safety, nutrition and great market potential. The antioxidant and anticancer properties of PPIs were evaluated with cell -based biological assays. The results showed that when the concentration of PPIs was 5 mg/mL, the peroxyl radical scavenging value was (2 119 +/- 204) mg VCE/100 g, and the cellular antioxidant activity values were (45.83 +/- 3.5) (no PBS wash) and (33.25 +/- 4.4) mu mol QE/100 g (PBS wash). Cells pretreated with PPIs could significantly prevent the oxidative damage induced by H2O2, inhibit the morphological changes of cells and maintain the integrity. Furthermore, PPIs had selective anti-proliferative effects on GIST882 cells (IC50 = (10.53 +/- 3.87) mg/mL) and demonstrated potent inhibition of the growth, migration and invasion of cancer cells. These findings provide a scientific basis for PPIs as promising candidates for functional foods to aid in the prevention of oxidative damage and cancer.(c) 2023 Beijing Academy of Food Sciences. Publishing services by Elsevier B.V. on behalf of KeAi Communications Co., Ltd. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
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