Advances in Cas12a-Based Amplification-Free Nucleic Acid Detection

Rapid and sensitive point-of-care detection of Leptospira by RPA-CRISPR/Cas12a targeting lipL32

(2022) Sirawit Jirawannaporn et al.   PLoS Neglected Tropical Diseases

Harnessing Multiplex crRNA in the CRISPR/Cas12a System Enables an Amplification-Free DNA Diagnostic Platform for ASFV Detection

(2022) Muchu Zeng et al.   ANALYTICAL CHEMISTRY

Amplification-Free, Single-Microbead-Based Cas12a Assay for One-Step DNA Detection at the Single-Molecule Level

(2022) Xueping Yang et al.   ANALYTICAL CHEMISTRY

Rapid screening of antimicrobial probiotics using CRISPR cascade

(2022) Yunhao Lu et al.   BIOSENSORS & BIOELECTRONICS

Ultrasensitive and Highly Specific Lateral Flow Assays for Point-of-Care Diagnosis

(2021) Yilin Liu et al.   ACS Nano

Cas12a-based electrochemiluminescence biosensor for target amplification-free DNA detection

(2021) Peng-Fei Liu et al.   BIOSENSORS & BIOELECTRONICS

Integral Multielement Signals by DNA-Programmed UCNP–AuNP Nanosatellite Assemblies for Ultrasensitive ICP–MS Detection of Exosomal Proteins and Cancer Identification

(2021) Xue-Wei Zhang et al.   ANALYTICAL CHEMISTRY

Digital CRISPR-based Method for the Rapid Detection and Absolute Quantification of Nucleic Acids

(2021) Xiaolin Wu et al.   BIOMATERIALS

Clustered Regularly Interspaced Short Palindromic Repeats-Mediated Amplification-Free Detection of Viral DNAs Using Surface-Enhanced Raman Spectroscopy-Active Nanoarray

(2021) Jin-Ha Choi et al.   ACS Nano

G-triplex: A new type of CRISPR-Cas12a reporter enabling highly sensitive nucleic acid detection

(2021) Tao Li et al.   BIOSENSORS & BIOELECTRONICS

Droplet Cas12a Assay Enables DNA Quantification from Unamplified Samples at the Single-Molecule Level

(2021) Huahua Yue et al.   NANO LETTERS

Application of the amplification-free SERS-based CRISPR/Cas12a platform in the identification of SARS-CoV-2 from clinical samples

(2021) Jiajie Liang et al.   JOURNAL OF NANOBIOTECHNOLOGY

Electric field-enhanced electrochemical CRISPR biosensor for DNA detection

(2021) Ziyue Li et al.   BIOSENSORS & BIOELECTRONICS

SERS-based test strips: Principles, designs and applications

(2021) Luyang Wang et al.   BIOSENSORS & BIOELECTRONICS

CRISPR-cas12a mediated SERS lateral flow assay for amplification-free detection of double-stranded DNA and single-base mutation

(2021) Yuanfeng Pang et al.   CHEMICAL ENGINEERING JOURNAL

CRISPR/Cas12a Powered DNA Framework‐Supported Electrochemical Biosensing Platform for Ultrasensitive Nucleic Acid Analysis

(2021) Jing Su et al.   Small Methods

Clustered Regularly Interspaced Short Palindromic Repeats/Cas9-Mediated Lateral Flow Nucleic Acid Assay

(2020) Xusheng Wang et al.   ACS Nano

CRISPR/Cas Systems in Genome Editing: Methodologies and Tools for sgRNA Design, Off‐Target Evaluation, and Strategies to Mitigate Off‐Target Effects

(2020) Hakim Manghwar et al.   Advanced Science

CRISPR/Cas12a-Mediated Interfacial Cleaving of Hairpin DNA Reporter for Electrochemical Nucleic Acid Sensing

(2020) Decai Zhang et al.   ACS Sensors

High-throughput and all-solution phase African Swine Fever Virus (ASFV) detection using CRISPR-Cas12a and fluorescence based point-of-care system

(2020) Qian He et al.   BIOSENSORS & BIOELECTRONICS

PAM recognition by miniature CRISPR–Cas12f nucleases triggers programmable double-stranded DNA target cleavage

(2020) Tautvydas Karvelis et al.   NUCLEIC ACIDS RESEARCH

End-point dual specific detection of nucleic acids using CRISPR/Cas12a based portable biosensor

(2020) Hui Wu et al.   BIOSENSORS & BIOELECTRONICS

Molecular diagnostic technologies for COVID-19: Limitations and challenges

(2020) Adeel Afzal   Journal of Advanced Research

Enhancement of trans-cleavage activity of Cas12a with engineered crRNA enables amplified nucleic acid detection

(2020) Long T. Nguyen et al.   Nature Communications

COVID-19: molecular and serological detection methods

(2020) Ahmed E. Dhamad et al.   PeerJ

CRISPR-Cas12a-Based Nucleic Acid Amplification-Free DNA Biosensor via Au Nanoparticle-Assisted Metal-Enhanced Fluorescence and Colorimetric Analysis

(2020) Jin-Ha Choi et al.   NANO LETTERS

Fabrication of ultrasensitive electrochemical biosensor for dengue fever viral RNA Based on CRISPR/Cpf1 reaction

(2020) Yeonju Lee et al.   SENSORS AND ACTUATORS B-CHEMICAL

Palindromic Fragment-Mediated Single-Chain Amplification: An Innovative Mode for Photoelectrochemical Bioassay

(2019) Ruijin Zeng et al.   ANALYTICAL CHEMISTRY

Recent Advances on Electrochemical Biosensing Strategies toward Universal Point of Care Systems

(2019) Yifan Dai et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Manipulating “Hot Spots” from Nanometer to Angstrom: Towards Understanding Integrated Contributions of Molecule Number and Gap Size for Ultrasensitive SERS Detection

(2019) Hui Lu et al.   ACS Applied Materials & Interfaces

Nondestructive Characterization of Stem Cell Neurogenesis by a Magneto-Plasmonic Nanomaterial-Based Exosomal miRNA Detection

(2019) Jin-Ho Lee et al.   ACS Nano

Exploring the Trans-Cleavage Activity of CRISPR-Cas12a (cpf1) for the Development of a Universal Electrochemical Biosensor

(2019) Yifan Dai et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Highly sensitive surface-enhanced Raman spectroscopy (SERS) platform using core/double shell (Ag/polymer/Ag) nanohorn for proteolytic biosensor

(2019) Jin Ha Choi et al.   APPLIED SURFACE SCIENCE

Evaluation of off‐target effects of gapmer antisense oligonucleotides using human cells

(2019) Tokuyuki Yoshida et al.   GENES TO CELLS

CRISPR-Cas12a target binding unleashes indiscriminate single-stranded DNase activity

(2018) Janice S. Chen et al.   SCIENCE

Raman enhancement on ultra-clean graphene quantum dots produced by quasi-equilibrium plasma-enhanced chemical vapor deposition

(2018) Donghua Liu et al.   Nature Communications

Raman Enhancement of Blood Constituent Proteins Using Graphene

(2018) Shengxi Huang et al.   ACS Photonics

CRISPR-Cas12a-assisted nucleic acid detection

(2018) Shi-Yuan Li et al.   Cell Discovery

Gold Nanoparticle Couples with Entropy-Driven Toehold-Mediated DNA Strand Displacement Reaction on Magnetic Beads: Toward Ultrasensitive Energy-Transfer-Based Photoelectrochemical Detection of miRNA-141 in Real Blood Sample

(2018) Nan Zhang et al.   ANALYTICAL CHEMISTRY

Potential-Resolved Multicolor Electrochemiluminescence for Multiplex Immunoassay in a Single Sample

(2018) Weiliang Guo et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Temperature-Robust DNAzyme Biosensors Confirming Ultralow Background Detection

(2018) Ruijie Deng et al.   ACS Sensors

Nicking enzyme-assisted amplification (NEAA) technology and its applications: A review

(2018) Cheng Qian et al.   ANALYTICA CHIMICA ACTA

Chemistry-Driven Approaches for Ultrasensitive Nucleic Acid Detection

(2017) Sarah J. Smith et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Nucleic acid detection with CRISPR-Cas13a/C2c2

(2017) Jonathan S. Gootenberg et al.   SCIENCE

High-Performance Ratiometric Electrochemical Method Based on the Combination of Signal Probe and Inner Reference Probe in One Hairpin-Structured DNA

(2016) Chunyan Deng et al.   ANALYTICAL CHEMISTRY

The CRISPR-associated DNA-cleaving enzyme Cpf1 also processes precursor CRISPR RNA

(2016) Ines Fonfara et al.   NATURE

C2c2 is a single-component programmable RNA-guided RNA-targeting CRISPR effector

(2016) Omar O. Abudayyeh et al.   SCIENCE

Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System

(2015) Bernd Zetsche et al.   CELL

DNAzyme-based biosensors and nanodevices

(2015) Liang Gong et al.   CHEMICAL COMMUNICATIONS

Silver nanoparticle enhanced Raman scattering-based lateral flow immunoassays for ultra-sensitive detection of the heavy metal chromium

(2014) Jiajie Liang et al.   NANOTECHNOLOGY

What is next generation sequencing?

(2013) Sam Behjati et al.   Archives of Disease in Childhood-Education and Practice Edition

3D graphene oxide-encapsulated gold nanoparticles to detect neural stem cell differentiation

(2013) Tae-Hyung Kim et al.   BIOMATERIALS

Rapid Detection and Limitations of Molecular Techniques

(2012) John J. Maurer   Annual Review of Food Science and Technology

Small CRISPR RNAs Guide Antiviral Defense in Prokaryotes

(2008) S. J. J. Brouns et al.   SCIENCE