Digital CRISPR-based Method for the Rapid Detection and Absolute Quantification of Nucleic Acids

An engineered CRISPR-Cas12a variant and DNA-RNA hybrid guides enable robust and rapid COVID-19 testing

(2021) Kean Hean Ooi et al.   Nature Communications

SARS-CoV-2 Viral Load in Upper Respiratory Specimens of Infected Patients

(2020) Lirong Zou et al.   NEW ENGLAND JOURNAL OF MEDICINE

Quantitative Detection and Viral Load Analysis of SARS-CoV-2 in Infected Patients

(2020) Fengting Yu et al.   CLINICAL INFECTIOUS DISEASES

Aerodynamic analysis of SARS-CoV-2 in two Wuhan hospitals

(2020) Yuan Liu et al.   NATURE

Massively multiplexed nucleic acid detection with Cas13

(2020) Cheri M. Ackerman et al.   NATURE

Virological assessment of hospitalized patients with COVID-2019

(2020) Roman Wölfel et al.   NATURE

Viral contamination in biologic manufacture and implications for emerging therapies

(2020) Paul W. Barone et al.   NATURE BIOTECHNOLOGY

CRISPR–Cas12-based detection of SARS-CoV-2

(2020) James P. Broughton et al.   NATURE BIOTECHNOLOGY

Analytical comparisons of SARS-COV-2 detection by qRT-PCR and ddPCR with multiple primer/probe sets

(2020) Xinjin Liu et al.   Emerging Microbes & Infections

SARS-CoV-2 viral load predicts COVID-19 mortality

(2020) Elisabet Pujadas et al.   Lancet Respiratory Medicine

Utilization of Digital PCR in Quantity Verification of Plasmid Standards Used in Quantitative PCR

(2020) Martina Beinhauerova et al.   Frontiers in Molecular Biosciences

Extrachromosomal DNA is associated with oncogene amplification and poor outcome across multiple cancers

(2020) Hoon Kim et al.   NATURE GENETICS

Detection and quantification of SARS-CoV-2 by droplet digital PCR in real-time PCR negative nasopharyngeal swabs from suspected COVID-19 patients

(2020) Claudia Alteri et al.   PLoS One

Ultrasensitive and visual detection of SARS-CoV-2 using all-in-one dual CRISPR-Cas12a assay

(2020) Xiong Ding et al.   Nature Communications

Development and evaluation of a rapid CRISPR-based diagnostic for COVID-19

(2020) Tieying Hou et al.   PLoS Pathogens

An Ultralocalized Cas13a Assay Enables Universal and Nucleic Acid Amplification-Free Single-Molecule RNA Diagnostics

(2020) Tian Tian et al.   ACS Nano

CRISPR/Cas Systems towards Next-Generation Biosensing

(2019) Yi Li et al.   TRENDS IN BIOTECHNOLOGY

Digital Loop-Mediated Isothermal Amplification on a Commercial Membrane

(2019) Xingyu Lin et al.   ACS Sensors

Reduced False Positives and Improved Reporting of Loop-Mediated Isothermal Amplification using Quenched Fluorescent Primers

(2019) Patrick Hardinge et al.   Scientific Reports

Viral Detection: Past, Present, and Future

(2019) Konstantina Katsarou et al.   BIOESSAYS

SHERLOCK: nucleic acid detection with CRISPR nucleases

(2019) Max J. Kellner et al.   Nature Protocols

HOLMESv2: A CRISPR-Cas12b-Assisted Platform for Nucleic Acid Detection and DNA Methylation Quantitation

(2019) Linxian Li et al.   ACS Synthetic Biology

Digital PCR—An Emerging Technology with Broad Applications in Microbiology

(2019) Stephen J. Salipante et al.   CLINICAL CHEMISTRY

A comparison of EBV serology and serum cell‐free DNA as screening tools for nasopharyngeal cancer: results of the Singapore NPC screening cohort

(2019) Joshua K Tay et al.   INTERNATIONAL JOURNAL OF CANCER

Smartphone-Based Droplet Digital LAMP Device with Rapid Nucleic Acid Isolation for Highly Sensitive Point-of-Care Detection

(2019) Fei Hu et al.   ANALYTICAL CHEMISTRY

Rethinking liquid biopsy: Microfluidic assays for mobile tumor cells in human body fluids

(2018) Kuang Hong Neoh et al.   BIOMATERIALS

Digital quantification of DNA via isothermal amplification on a self-driven microfluidic chip featuring hydrophilic film-coated polydimethylsiloxane

(2018) Yu-Dong Ma et al.   BIOSENSORS & BIOELECTRONICS

CRISPR-Cas12a has both cis- and trans-cleavage activities on single-stranded DNA

(2018) Shi-Yuan Li et al.   CELL RESEARCH

Field-deployable viral diagnostics using CRISPR-Cas13

(2018) Cameron Myhrvold et al.   SCIENCE

CRISPR-Cas12a target binding unleashes indiscriminate single-stranded DNase activity

(2018) Janice S. Chen et al.   SCIENCE

Multiplexed and portable nucleic acid detection platform with Cas13, Cas12a, and Csm6

(2018) Jonathan S. Gootenberg et al.   SCIENCE

Recombinase polymerase amplification: Basics, applications and recent advances

(2018) Ivan Magriñá Lobato et al.   TRAC-TRENDS IN ANALYTICAL CHEMISTRY

CRISPR-Cas12a-assisted nucleic acid detection

(2018) Shi-Yuan Li et al.   Cell Discovery

Real-Time, Digital LAMP with Commercial Microfluidic Chips Reveals the Interplay of Efficiency, Speed, and Background Amplification as a Function of Reaction Temperature and Time

(2018) Justin C. Rolando et al.   ANALYTICAL CHEMISTRY

GISAID: Global initiative on sharing all influenza data – from vision to reality

(2017) Yuelong Shu et al.   Eurosurveillance

Nucleic acid detection with CRISPR-Cas13a/C2c2

(2017) Jonathan S. Gootenberg et al.   SCIENCE

Self-powered integrated microfluidic point-of-care low-cost enabling (SIMPLE) chip

(2017) Erh-Chia Yeh et al.   Science Advances

Clarity™ digital PCR system: a novel platform for absolute quantification of nucleic acids

(2016) Huiyu Low et al.   ANALYTICAL AND BIOANALYTICAL CHEMISTRY

Progress in Quantitative Viral Load Testing: Variability and Impact of the WHO Quantitative International Standards

(2016) R. T. Hayden et al.   JOURNAL OF CLINICAL MICROBIOLOGY

Mobile suitcase laboratory for rapid detection of Leishmania donovani using recombinase polymerase amplification assay

(2016) Dinesh Mondal et al.   Parasites & Vectors

Comparison of Circulating Tumour Cells and Circulating Cell-Free Epstein-Barr Virus DNA in Patients with Nasopharyngeal Carcinoma Undergoing Radiotherapy

(2016) Jess Honganh Vo et al.   Scientific Reports

Inhibition of Recombinase Polymerase Amplification by Background DNA: A Lateral Flow-Based Method for Enriching Target DNA

(2015) Brittany Rohrman et al.   ANALYTICAL CHEMISTRY

Development of a rapid recombinase polymerase amplification assay for the detection of Streptococcus pneumoniae in whole blood

(2015) Eoin Clancy et al.   BMC INFECTIOUS DISEASES

Isothermal Amplification of Nucleic Acids

(2015) Yongxi Zhao et al.   CHEMICAL REVIEWS

Centrifugal step emulsification applied for absolute quantification of nucleic acids by digital droplet RPA

(2015) Friedrich Schuler et al.   LAB ON A CHIP

Influence of sequence mismatches on the specificity of recombinase polymerase amplification technology

(2015) Rana K. Daher et al.   MOLECULAR AND CELLULAR PROBES

Comparison of four digital PCR platforms for accurate quantification of DNA copy number of a certified plasmid DNA reference material

(2015) Lianhua Dong et al.   Scientific Reports

Evaluation of droplet digital PCR for characterizing plasmid reference material used for quantifying ammonia oxidizers and denitrifiers

(2014) Lianhua Dong et al.   ANALYTICAL AND BIOANALYTICAL CHEMISTRY

Comparative Study of Sensitivity, Linearity, and Resistance to Inhibition of Digital and Nondigital Polymerase Chain Reaction and Loop Mediated Isothermal Amplification Assays for Quantification of Human Cytomegalovirus

(2014) Gavin Nixon et al.   ANALYTICAL CHEMISTRY

Absolute quantification by droplet digital PCR versus analog real-time PCR

(2013) Christopher M Hindson et al.   NATURE METHODS

Methods for Applying Accurate Digital PCR Analysis on Low Copy DNA Samples

(2013) Alexandra S. Whale et al.   PLoS One

Viral diagnostics in the era of digital polymerase chain reaction

(2012) Ruth Hall Sedlak et al.   DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE

Digital PCR hits its stride

(2012) Monya Baker   NATURE METHODS

Comparison of microfluidic digital PCR and conventional quantitative PCR for measuring copy number variation

(2012) Alexandra S. Whale et al.   NUCLEIC ACIDS RESEARCH

Digital Isothermal Quantification of Nucleic Acids via Simultaneous Chemical Initiation of Recombinase Polymerase Amplification Reactions on SlipChip

(2011) Feng Shen et al.   ANALYTICAL CHEMISTRY

Serious Overestimation in Quantitative PCR by Circular (Supercoiled) Plasmid Standard: Microalgal pcna as the Model Gene

(2010) Yubo Hou et al.   PLoS One