Opposite Enantioselectivity in the Bioreduction of (Z )-β-Aryl-β-cyanoacrylates Mediated by the Tryptophan 116 Mutants of Old Yellow Enzyme 1: Synthetic Approach to (R )- and (S )-β-Aryl-γ-lactams

RApid Parallel Protein EvaluatoR (RAPPER), from gene to enzyme function in one day

(2015) L. T. Quertinmont et al.   CHEMICAL COMMUNICATIONS

Structural and Functional Consequences of Circular Permutation on the Active Site of Old Yellow Enzyme

(2015) Ashley B. Daugherty et al.   ACS Catalysis

Enantioselective Synthesis of (R)-2-Arylpropanenitriles Catalysed by Ene-Reductases in Aqueous Media and in Biphasic Ionic Liquid-Water Systems

(2014) Elisabetta Brenna et al.   ChemCatChem

How Green is Biocatalysis? To Calculate is To Know

(2014) Yan Ni et al.   ChemCatChem

Enzymatic hydrogenation of diverse activated alkenes. Identification of two Bacillus old yellow enzymes with broad substrate profiles

(2014) Hailing Zhang et al.   JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC

Rationalisation of the stereochemical outcome of ene-reductase-mediated bioreduction of α,β-difunctionalised alkenes

(2014) Elisabetta Brenna et al.   JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC

Identification of promiscuous ene-reductase activity by mining structural databases using active site constellations

(2014) Georg Steinkellner et al.   Nature Communications

Residues Controlling Facial Selectivity in an Alkene Reductase and Semirational Alterations to Create Stereocomplementary Variants

(2014) Adam Z. Walton et al.   ACS Catalysis

Chemoenzymatic Asymmetric Synthesis of Pregabalin Precursors via Asymmetric Bioreduction of β-Cyanoacrylate Esters Using Ene-Reductases

(2013) Christoph K. Winkler et al.   JOURNAL OF ORGANIC CHEMISTRY

Variations in the stability of NCR ene reductase by rational enzyme loop modulation

(2013) Sabrina Reich et al.   JOURNAL OF STRUCTURAL BIOLOGY

Improved Biocatalysts from a Synthetic Circular Permutation Library of the Flavin-Dependent Oxidoreductase Old Yellow Enzyme

(2013) Ashley B. Daugherty et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Biocatalytic retrosynthesis

(2013) Nicholas J Turner et al.   Nature Chemical Biology

Evaluation of Several Routes to Advanced Pregabalin Intermediates: Synthesis and Enantioselective Enzymatic Reduction Using Ene-Reductases

(2013) Sébastien Debarge et al.   ORGANIC PROCESS RESEARCH & DEVELOPMENT

Biocatalytic Asymmetric Alkene Reduction: Crystal Structure and Characterization of a Double Bond Reductase from Nicotiana tabacum

(2013) David J. Mansell et al.   ACS Catalysis

X-ray Crystallography Reveals How Subtle Changes Control the Orientation of Substrate Binding in an Alkene Reductase

(2013) Yuri A. Pompeu et al.   ACS Catalysis

Enzyme engineering toolbox – a ‘catalyst’ for change

(2013) Helen S. Toogood et al.   Catalysis Science & Technology

Old Yellow Enzyme-mediated reduction of β-cyano-α,β-unsaturated esters for the synthesis of chiral building blocks: stereochemical analysis of the reaction

(2013) Elisabetta Brenna et al.   Catalysis Science & Technology

Steric Effects on the Stereochemistry of Old Yellow Enzyme-Mediated Reductions of Unsaturated Diesters: Flipping of the Substrate within the Enzyme Active Site Induced by Structural Modifications

(2012) Elisabetta Brenna et al.   ADVANCED SYNTHESIS & CATALYSIS

Stereochemical Outcome of the Biocatalysed Reduction of Activated Tetrasubstituted Olefins by Old Yellow Enzymes 1-3

(2012) Elisabetta Brenna et al.   ADVANCED SYNTHESIS & CATALYSIS

A Three-Enzyme System Involving an Ene-Reductase for Generating Valuable Chiral Building Blocks

(2012) David Mangan et al.   ADVANCED SYNTHESIS & CATALYSIS

Structural and Catalytic Characterization of Pichia stipitis OYE 2.6, a Useful Biocatalyst for Asymmetric Alkene Reductions

(2012) Yuri A. Pompeu et al.   ADVANCED SYNTHESIS & CATALYSIS

Asymmetric bioreduction of activated alkenes by a novel isolate of Achromobacter species producing enoate reductase

(2012) Yan-Jie Liu et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Nicotinamide-Dependent Ene Reductases as Alternative Biocatalysts for the Reduction of Activated Alkenes

(2012) Katharina Durchschein et al.   EUROPEAN JOURNAL OF ORGANIC CHEMISTRY

Engineering the third wave of biocatalysis

(2012) U. T. Bornscheuer et al.   NATURE

Asymmetric bioreduction of activated carbon–carbon double bonds using Shewanella yellow enzyme (SYE-4) as novel enoate reductase

(2012) Naseem Iqbal et al.   TETRAHEDRON

Stereo-Controlled Asymmetric Bioreduction of α,β-Dehydroamino Acid Derivatives

(2011) Clemens Stueckler et al.   ADVANCED SYNTHESIS & CATALYSIS

Biocatalyzed Enantioselective Reduction of Activated C=C Bonds: Synthesis of Enantiomerically Enriched α-Halo-β-arylpropionic Acids

(2011) Elisabetta Brenna et al.   EUROPEAN JOURNAL OF ORGANIC CHEMISTRY

Enoate Reductase-Mediated Preparation of Methyl (S)-2-Bromobutanoate, a Useful Key Intermediate for the Synthesis of Chiral Active Pharmaceutical Ingredients

(2011) Elisabetta Brenna et al.   ORGANIC PROCESS RESEARCH & DEVELOPMENT

Biotechnological Development of a Practical Synthesis of Ethyl (S)-2-Ethoxy-3-(p-methoxyphenyl)propanoate (EEHP): Over 100-Fold Productivity Increase from Yeast Whole Cells to Recombinant Isolated Enzymes

(2011) Matthias Bechtold et al.   ORGANIC PROCESS RESEARCH & DEVELOPMENT

Biocatalytic Reductions and Chemical Versatility of the Old Yellow Enzyme Family of Flavoprotein Oxidoreductases

(2010) Helen S. Toogood et al.   ChemCatChem

Towards preparative-scale, biocatalytic alkene reductions

(2010) Despina J. Bougioukou et al.   CHEMICAL COMMUNICATIONS

Directed Evolution of an Enantioselective Enoate-Reductase: Testing the Utility of Iterative Saturation Mutagenesis

(2009) Despina J. Bougioukou et al.   ADVANCED SYNTHESIS & CATALYSIS

Site-Saturation Mutagenesis of Tryptophan 116 of Saccharomyces pastorianus Old Yellow Enzyme Uncovers Stereocomplementary Variants

(2009) Santosh Kumar Padhi et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Directed evolution drives the next generation of biocatalysts

(2009) Nicholas J Turner   Nature Chemical Biology

A short, chemoenzymatic route to chiral β-aryl-γ-amino acids using reductases from anaerobic bacteria

(2009) Anna Fryszkowska et al.   ORGANIC & BIOMOLECULAR CHEMISTRY

Asymmetric Bioreduction of Activated C=C Bonds UsingZymomonas mobilis NCR Enoate Reductase and Old Yellow Enzymes OYE 1–3 from Yeasts

(2008) Mélanie Hall et al.   EUROPEAN JOURNAL OF ORGANIC CHEMISTRY

Comparative pharmacological activity of optical isomers of phenibut

(2008) Maija Dambrova et al.   EUROPEAN JOURNAL OF PHARMACOLOGY

Asymmetric bioreduction of α,β-unsaturated nitriles and ketones

(2008) Birgit Kosjek et al.   TETRAHEDRON-ASYMMETRY