4.6 Article

Development of exosome-encapsulated paclitaxel to overcome MDR in cancer cells

Journal

NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE
Volume 12, Issue 3, Pages 655-664

Publisher

ELSEVIER SCIENCE BV
DOI: 10.1016/j.nano.2015.10.012

Keywords

Cancer; Drug resistance; Exosome; Paclitaxel; Pgp

Funding

  1. United States National Institutes of Health [1RO1 NS057748]
  2. UNC Eshelman School of Pharmacy through the Lineberger Comprehensive Cancer Center [RR021937]
  3. University Cancer Research Fund through the Lineberger Comprehensive Cancer Center [RR021937]
  4. Ministry of Education and Science of Russian Federation [11.G34.31.0004, 02.740.11.5232, RSF-14-13-00731]
  5. Russian Science Foundation [14-13-00731] Funding Source: Russian Science Foundation

Ask authors/readers for more resources

Exosomes have recently come into focus as natural nanoparticles for use as drug delivery vehicles. Our objective was to assess the feasibility of an exosome-based drug delivery platform for a potent chemotherapeutic agent, paclitaxel (PTX), to treat MDR cancer. Herein, we developed different methods of loading exosomes released by macrophages with PTX (exoPTX), and characterized their size, stability, drug release, and in vitro antitumor efficacy. Reformation of the exosomal membrane upon sonication resulted in high loading efficiency and sustained drug release. Importantly, incorporation of PTX into exosomes increased cytotoxicity more than 50 times in drug resistant MDCKMDR1 (Pgp+) cells. Next, our studies demonstrated a nearly complete co-localization of airway-delivered exosomes with cancer cells in a model of murine Lewis lung carcinoma pulmonary metastases, and a potent anticancer effect in this mouse model. We conclude that exoPTX holds significant potential for the delivery of various chemotherapeutics to treat drug resistant cancers. Published by Elsevier Inc.

Authors

I am an author on this paper
Click your name to claim this paper and add it to your profile.

Reviews

Primary Rating

4.6
Not enough ratings

Secondary Ratings

Novelty
-
Significance
-
Scientific rigor
-
Rate this paper

Recommended

No Data Available
No Data Available