Rapid characterization of anti-CRISPR proteins and optogenetically engineered variants using a versatile plasmid interference system

Anti-CRISPR Discovery: Using Magnets to Find Needles in Haystacks

(2023) Kevin J. Forsberg   JOURNAL OF MOLECULAR BIOLOGY

Diverse Mechanisms of CRISPR-Cas9 Inhibition by Type II Anti-CRISPR Proteins

(2023) Sungwon Hwang et al.   JOURNAL OF MOLECULAR BIOLOGY

Discovery of potent and versatile CRISPR–Cas9 inhibitors engineered for chemically controllable genome editing

(2022) Guoxu Song et al.   NUCLEIC ACIDS RESEARCH

Anti-CRISPR prediction using deep learning reveals an inhibitor of Cas13b nucleases

(2022) Katharina G. Wandera et al.   MOLECULAR CELL

AcrIF5 specifically targets DNA-bound CRISPR-Cas surveillance complex for inhibition

(2022) Yongchao Xie et al.   Nature Chemical Biology

Optogenetics for transcriptional programming and genetic engineering

(2022) Tien-Hung Lan et al.   TRENDS IN GENETICS

Chromothripsis as an on-target consequence of CRISPR–Cas9 genome editing

(2021) Mitchell L. Leibowitz et al.   NATURE GENETICS

Highly accurate protein structure prediction with AlphaFold

(2021) John Jumper et al.   NATURE

Structure-based functional mechanisms and biotechnology applications of anti-CRISPR proteins

(2021) Ning Jia et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Inhibition mechanisms of CRISPR-Cas9 by AcrIIA17 and AcrIIA18

(2021) Xiaoshen Wang et al.   NUCLEIC ACIDS RESEARCH

Prophage integration into CRISPR loci enables evasion of antiviral immunity in Streptococcus pyogenes

(2021) Andrew Varble et al.   Nature Microbiology

Coupling Cas9 to artificial inhibitory domains enhances CRISPR-Cas9 target specificity

(2020) Sabine Aschenbrenner et al.   Science Advances

Anti-CRISPR protein applications: natural brakes for CRISPR-Cas technologies

(2020) Nicole D. Marino et al.   NATURE METHODS

Potent CRISPR-Cas9 inhibitors fromStaphylococcusgenomes

(2020) Kyle E. Watters et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Broad-spectrum anti-CRISPR proteins facilitate horizontal gene transfer

(2020) Caroline Mahendra et al.   Nature Microbiology

Optogenetic control of Neisseria meningitidis Cas9 genome editing using an engineered, light-switchable anti-CRISPR protein

(2020) Mareike D Hoffmann et al.   NUCLEIC ACIDS RESEARCH

Diverse Mechanisms of CRISPR-Cas9 Inhibition by Type IIC Anti-CRISPR Proteins

(2019) Yalan Zhu et al.   MOLECULAR CELL

Discovery and Characterization of Cas9 Inhibitors Disseminated across Seven Bacterial Phyla

(2019) Ruben V. Uribe et al.   Cell Host & Microbe

Cell-specific CRISPR–Cas9 activation by microRNA-dependent expression of anti-CRISPR proteins

(2019) Mareike D Hoffmann et al.   NUCLEIC ACIDS RESEARCH

Tissue-restricted Genome Editing in vivo Specified by MicroRNA-repressible Anti-CRISPR Proteins

(2019) Jooyoung Lee et al.   RNA

Functional metagenomics-guided discovery of potent Cas9 inhibitors in the human microbiome

(2019) Kevin J Forsberg et al.   eLife

Search-and-replace genome editing without double-strand breaks or donor DNA

(2019) Andrew V. Anzalone et al.   NATURE

AcrIIA5 Inhibits a Broad Range of Cas9 Orthologs by Preventing DNA Target Cleavage

(2019) Guoxu Song et al.   Cell Reports

Evolutionary classification of CRISPR–Cas systems: a burst of class 2 and derived variants

(2019) Kira S. Makarova et al.   NATURE REVIEWS MICROBIOLOGY

The Biology of CRISPR-Cas: Backward and Forward

(2018) Frank Hille et al.   CELL

A Completely Reimplemented MPI Bioinformatics Toolkit with a New HHpred Server at its Core

(2018) Lukas Zimmermann et al.   JOURNAL OF MOLECULAR BIOLOGY

Repair of double-strand breaks induced by CRISPR–Cas9 leads to large deletions and complex rearrangements

(2018) Michael Kosicki et al.   NATURE BIOTECHNOLOGY

The CRISPR tool kit for genome editing and beyond

(2018) Mazhar Adli   Nature Communications

CRISPR-Cas guides the future of genetic engineering

(2018) Gavin J. Knott et al.   SCIENCE

Engineered anti-CRISPR proteins for optogenetic control of CRISPR–Cas9

(2018) Felix Bubeck et al.   NATURE METHODS

Base editing: precision chemistry on the genome and transcriptome of living cells

(2018) Holly A. Rees et al.   NATURE REVIEWS GENETICS

Structural basis of CRISPR–SpyCas9 inhibition by an anti-CRISPR protein

(2017) De Dong et al.   NATURE

Diversity and evolution of class 2 CRISPR–Cas systems

(2017) Sergey Shmakov et al.   NATURE REVIEWS MICROBIOLOGY

Disabling Cas9 by an anti-CRISPR DNA mimic

(2017) Jiyung Shin et al.   Science Advances

Naturally Occurring Off-Switches for CRISPR-Cas9

(2016) April Pawluk et al.   CELL

Profiling of engineering hotspots identifies an allosteric CRISPR-Cas9 switch

(2016) Benjamin L Oakes et al.   NATURE BIOTECHNOLOGY

Multiple mechanisms for CRISPR–Cas inhibition by anti-CRISPR proteins

(2015) Joseph Bondy-Denomy et al.   NATURE

Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression

(2013) Lei S. Qi et al.   CELL

DNA targeting specificity of RNA-guided Cas9 nucleases

(2013) Patrick D Hsu et al.   NATURE BIOTECHNOLOGY

Bacteriophage genes that inactivate the CRISPR/Cas bacterial immune system

(2012) Joe Bondy-Denomy et al.   NATURE

CMView: Interactive contact map visualization and analysis

(2011) C. Vehlow et al.   BIOINFORMATICS

CRISPR/Cas, the Immune System of Bacteria and Archaea

(2010) P. Horvath et al.   SCIENCE

Jalview Version 2--a multiple sequence alignment editor and analysis workbench

(2009) A. M. Waterhouse et al.   BIOINFORMATICS