Article
Multidisciplinary Sciences
Anna Urciuolo, Giovanni Giuseppe Giobbe, Yixiao Dong, Federica Michielin, Luca Brandolino, Michael Magnussen, Onelia Gagliano, Giulia Selmin, Valentina Scattolini, Paolo Raffa, Paola Caccin, Soichi Shibuya, Dominic Scaglioni, Xuechun Wang, Ju Qu, Marko Nikolic, Marco Montagner, Gabriel L. Galea, Hans Clevers, Monica Giomo, Paolo De Coppi, Nicola Elvassore
Summary: This study develops a hydrogel-in-hydrogel bioprinting approach to dynamically control the growth landscape of three-dimensional cell cultures. The technique enables modulation of hydrogel characteristics to guide neural axon directionality, control cell migration in cancer organoids, and promote cell polarity in liver and small intestinal organoids, as well as control lung tip bifurcation.
NATURE COMMUNICATIONS
(2023)
Review
Biochemistry & Molecular Biology
Herbert Schneckenburger
Summary: Laser plays an increasing role in live cell microscopy due to its unique properties. It is used in various techniques such as 3D microscopy, super-resolution microscopy, spectral imaging, and fluorescence lifetime imaging. Laser applications in live cell biology include fluorescence diagnosis, photodynamic therapy, and laser-assisted optoporation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
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Chemistry, Physical
Ilya D. Solovyev, Liliya G. Maloshenok, Alexander P. Savitsky
Summary: This study demonstrates the use of fluorescent correlation spectroscopy (FCS) to measure protein interactions and changes in oligomeric state. The researchers controlled the amount of fluorescent dye using a photoconvertible protein, SAASoti. They validated the use of photoconvertible proteins for measuring caspase enzymatic activity in live cells. By using a specially engineered FRET sensor expressed in HeLa cells, they were able to detect caspase-3 activity and its correlation with changes in cell viscosity during apoptosis.
Article
Microscopy
Julia M. T. Auer, Laura C. Murphy, Dong Xiao, David U. Li, Ann P. Wheeler
Summary: Molecular interactions are crucial in cellular processes, especially in gene regulation. Non-fitting lifetime-based FRET variants, such as AAT-FRET, offer a sensitive and simple method to detect and measure protein-protein interactions in live 3D samples.
JOURNAL OF MICROSCOPY
(2023)
Article
Chemistry, Physical
Pratibha Agarwala, Turban Bera, Dibyendu K. Sasmal
Summary: This study reports the mechanism of interaction between curcumin and bovine serum albumin (BSA) as well as live E. coli cell membrane. The results show the formation of complexes between curcumin and BSA and the bacterial membrane, indicating a strong hydrophobic interaction. These findings shed light on the potential applications of curcumin as a natural therapeutic lead in clinical trials against life-threatening diseases.
Article
Instruments & Instrumentation
Seyda Tugce Durhan, Enise Nalli Sezer, Cagdas Devrim Son, Fatma Kucuk Baloglu
Summary: Protein-protein interactions are vital for intracellular signaling and maintaining organism homeostasis. Forster resonance energy transfer (FRET) is a powerful tool for detecting these interactions in living cells, but current analysis methods are expensive and time-consuming. To address these issues, a rapid, low-cost, and effective FRET-based assay method is proposed using a standard microplate reader and an alternative calculation procedure.
APPLIED SPECTROSCOPY
(2023)
Article
Neurosciences
Chiara Salio, Adalberto Merighi, Rita Bardoni
Review
Neurosciences
Adalberto Merighi
PROGRESS IN NEUROBIOLOGY
(2018)
Article
Neurosciences
Adalberto Merighi
FRONTIERS IN CELLULAR NEUROSCIENCE
(2018)
Review
Biochemistry & Molecular Biology
Laura Lossi, Claudia Castagna, Adalberto Merighi
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2018)
Article
Cell Biology
Claudia Castagna, Adalberto Merighi, Laura Lossi
CELLULAR AND MOLECULAR NEUROBIOLOGY
(2019)
Review
Medicine, General & Internal
Laura Lossi, Claudia Castagna, Alberto Granato, Adalberto Merighi
JOURNAL OF CLINICAL MEDICINE
(2019)
Article
Neurosciences
Maria Martinez-Lage, Timothy M. Lynch, Yingtao Bi, Carolina Cocito, Gregory P. Way, Sharmistha Pal, Josephine Haller, Rachel E. Yan, Amy Ziober, Aivi Nguyen, Manoj Kandpal, Donald M. O'Rourke, Jeffrey P. Greenfield, Casey S. Greene, Ramana V. Davuluri, Nadia Dahmane
ACTA NEUROPATHOLOGICA COMMUNICATIONS
(2019)
Article
Cell Biology
Chiara Salio, Patrizia Aimar, Pascale Malapert, Aziz Moqrich, Adalberto Merighi
Summary: The study investigated the immunocytochemistry of murine C-type DRG neurons, revealing the expression patterns of specific markers in different subpopulations. Using transgenic mice, the researchers examined the effects of selective ablation of certain neurons on the distribution and density of glomerular terminals, providing insights into interspecies differences and functional relevance.
CELLULAR AND MOLECULAR NEUROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Laura Lossi, Adalberto Merighi, Vittorino Novello, Alessandra Ferrandino
Article
Cell Biology
Alberto Granato, Adalberto Merighi
Summary: Pyramidal neurons are the most abundant cells in the neocortex and their dendrites play a crucial role in various neurological and mental disorders. Different types of intellectual disabilities may be sustained by different parts of the pyramidal neuron dendritic tree or by dysregulation of their interaction.
CELLULAR AND MOLECULAR NEUROBIOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Adalberto Merighi, Nadia Gionchiglia, Alberto Granato, Laura Lossi
Summary: The gamma phosphorylated form of histone H2AX, known as gamma H2AX, was discovered over 40 years ago and has been found to play a crucial role in the cellular response to DNA damage. It is involved in various cellular functions in both normal and pathological cells. Particularly, its involvement in the central nervous system, from immature to mature neurons, highlights its importance in neurogenesis and neuronal cell death, showcasing its pleiotropic nature with diverse functions from embryonic life to old age.
Review
Biochemistry & Molecular Biology
Adalberto Merighi, Laura Lossi
Summary: In addition to protein processing, the endoplasmic reticulum (ER) also plays roles in lipid synthesis, molecular transfers, and Ca2+ homeostasis. Proper protein folding and modification in the ER require molecular chaperones and a favorable environment. Disruption in ER conditions or chaperone capacity can lead to the unfolded protein response (UPR), triggering autophagy and potentially causing cell death.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Chelsea R. Wood, Esri H. Juarez, Francesco Ferrini, Peter Myint, John Innes, Laura Lossi, Adalberto Merighi, William E. B. Johnson
Summary: This study found that MSC conditioned medium had significant effects on spinal cord tissue in ex vivo slice cultures, enhancing viability and promoting GFAP immunoreactivity, contradicting previous findings.
BIOCHEMISTRY AND BIOPHYSICS REPORTS
(2021)
Article
Clinical Neurology
A. Wallin, E. Kapaki, M. Boban, S. Engelborghs, D. M. Hermann, B. Huisa, M. Jonsson, M. G. Kramberger, L. Lossi, B. Malojcic, S. Mehrabian, A. Merighi, E. B. Mukaetova-Ladinska, G. P. Paraskevas, B. O. Popescu, R. Ravid, L. Traykov, G. Tsivgoulis, G. Weinstein, A. Korczyn, M. Bjerke, G. Rosenberg
Review
Veterinary Sciences
Laura Lossi, Adalberto Merighi
FRONTIERS IN VETERINARY SCIENCE
(2018)