The changing landscape of membrane protein structural biology through developments in electron microscopy

Structural Insights into the Niemann-Pick C1 (NPC1)-Mediated Cholesterol Transfer and Ebola Infection

(2016) Xin Gong et al.   CELL

Breaking Cryo-EM Resolution Barriers to Facilitate Drug Discovery

(2016) Alan Merk et al.   CELL

Scipion: A software framework toward integration, reproducibility and validation in 3D electron microscopy

(2016) J.M. de la Rosa-Trevín et al.   JOURNAL OF STRUCTURAL BIOLOGY

Gctf: Real-time CTF determination and correction

(2016) Kai Zhang   JOURNAL OF STRUCTURAL BIOLOGY

Nonfouling NTA-PEG-Based TEM Grid Coatings for Selective Capture of Histidine-Tagged Protein Targets from Cell Lysates

(2016) Christopher J. Benjamin et al.   LANGMUIR

Methods to account for movement and flexibility in cryo-EM data processing

(2016) S. Rawson et al.   METHODS

Antibody-based affinity cryo-EM grid

(2016) Guimei Yu et al.   METHODS

An introduction to sample preparation and imaging by cryo-electron microscopy for structural biology

(2016) Rebecca F. Thompson et al.   METHODS

Continuous changes in structure mapped by manifold embedding of single-particle data in cryo-EM

(2016) Joachim Frank et al.   METHODS

A mechanism of viral immune evasion revealed by cryo-EM analysis of the TAP transporter

(2016) Michael L. Oldham et al.   NATURE

TRPV1 structures in nanodiscs reveal mechanisms of ligand and lipid action

(2016) Yuan Gao et al.   NATURE

How good can cryo-EM become?

(2016) Robert M Glaeser   NATURE METHODS

The development of cryo-EM into a mainstream structural biology technique

(2016) Eva Nogales   NATURE METHODS

A saposin-lipoprotein nanoparticle system for membrane proteins

(2016) Jens Frauenfeld et al.   NATURE METHODS

A method for detergent-free isolation of membrane proteins in their local lipid environment

(2016) Sarah C Lee et al.   Nature Protocols

Models for the a subunits of theThermus thermophilusV/A-ATPase andSaccharomyces cerevisiaeV-ATPase enzymes by cryo-EM and evolutionary covariance

(2016) Daniel G. Schep et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Volta phase plate cryo-EM of the small protein complex Prx3

(2016) Maryam Khoshouei et al.   Nature Communications

The use of SMALPs as a novel membrane protein scaffold for structure study by negative stain electron microscopy

(2015) Vincent Postis et al.   BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES

The styrene–maleic acid copolymer: a versatile tool in membrane research

(2015) Jonas M. Dörr et al.   EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS

Automatic estimation and correction of anisotropic magnification distortion in electron microscopes

(2015) Timothy Grant et al.   JOURNAL OF STRUCTURAL BIOLOGY

Alignment of cryo-EM movies of individual particles by optimization of image translations

(2015) John L. Rubinstein et al.   JOURNAL OF STRUCTURAL BIOLOGY

Semi-automated selection of cryo-EM particles in RELION-1.3

(2015) Sjors H.W. Scheres   JOURNAL OF STRUCTURAL BIOLOGY

Structure of the TRPA1 ion channel suggests regulatory mechanisms

(2015) Candice E. Paulsen et al.   NATURE

Electron cryomicroscopy observation of rotational states in a eukaryotic V-ATPase

(2015) Jianhua Zhao et al.   NATURE

An atomic structure of human γ-secretase

(2015) Xiao-chen Bai et al.   NATURE

Horizontal membrane-intrinsic α-helices in the stator a-subunit of an F-type ATP synthase

(2015) Matteo Allegretti et al.   NATURE

Architecture of the mammalian mechanosensitive Piezo1 channel

(2015) Jingpeng Ge et al.   NATURE

A structure of the COPI coat and the role of coat proteins in membrane vesicle assembly

(2015) S. O. Dodonova et al.   SCIENCE

Structure of the voltage-gated calcium channel Cav1.1 complex

(2015) J. Wu et al.   SCIENCE

GraDeR: Membrane Protein Complex Preparation for Single-Particle Cryo-EM

(2015) Florian Hauer et al.   STRUCTURE

Structure of the Vacuolar H + -ATPase Rotary Motor Reveals New Mechanistic Insights

(2015) Shaun Rawson et al.   STRUCTURE

How cryo-EM is revolutionizing structural biology

(2015) Xiao-chen Bai et al.   TRENDS IN BIOCHEMICAL SCIENCES

Low cost, high performance processing of single particle cryo-electron microscopy data in the cloud

(2015) Michael A Cianfrocco et al.   eLife

Measuring the optimal exposure for single particle cryo-EM using a 2.6 Å reconstruction of rotavirus VP6

(2015) Timothy Grant et al.   eLife

Structure and conformational states of the bovine mitochondrial ATP synthase by cryo-EM

(2015) Anna Zhou et al.   eLife

Sampling the conformational space of the catalytic subunit of human γ-secretase

(2015) Xiao-chen Bai et al.   eLife

Collaborative Computational Project for Electron cryo-Microscopy

(2015) Chris Wood et al.   ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY

A comprehensive review of the lipid cubic phase orin mesomethod for crystallizing membrane and soluble proteins and complexes

(2015) Martin Caffrey   Acta Crystallographica Section F-Structural Biology Communications

Folding and stability of integral membrane proteins in amphipols

(2014) Jörg H. Kleinschmidt et al.   ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS

Structure of a mammalian ryanodine receptor

(2014) Ran Zalk et al.   NATURE

Three-dimensional structure of human γ-secretase

(2014) Peilong Lu et al.   NATURE

Structure of the rabbit ryanodine receptor RyR1 at near-atomic resolution

(2014) Zhen Yan et al.   NATURE

Architecture and conformational switch mechanism of the ryanodine receptor

(2014) Rouslan G. Efremov et al.   NATURE

A general protocol for the generation of Nanobodies for structural biology

(2014) Els Pardon et al.   Nature Protocols

Trajectories of the ribosome as a Brownian nanomachine

(2014) Ali Dashti et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Volta potential phase plate for in-focus phase contrast transmission electron microscopy

(2014) R. Danev et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Comparison of optimal performance at 300keV of three direct electron detectors for use in low dose electron microscopy

(2014) G. McMullan et al.   ULTRAMICROSCOPY

Structural biology in situ—the potential of subtomogram averaging

(2013) John AG Briggs   CURRENT OPINION IN STRUCTURAL BIOLOGY

Cryo-electron tomography: The challenge of doing structural biology in situ

(2013) Vladan Lučić et al.   JOURNAL OF CELL BIOLOGY

Thermostabilisation of the Serotonin Transporter in a Cocaine-Bound Conformation

(2013) Saba Abdul-Hussein et al.   JOURNAL OF MOLECULAR BIOLOGY

Electron counting and beam-induced motion correction enable near-atomic-resolution single-particle cryo-EM

(2013) Xueming Li et al.   NATURE METHODS

Flexibility within the Rotor and Stators of the Vacuolar H+-ATPase

(2013) Chun Feng Song et al.   PLoS One

Xerocytosis is caused by mutations that alter the kinetics of the mechanosensitive channel PIEZO1

(2013) C. Bae et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Data management challenges in three-dimensional EM

(2012) Ardan Patwardhan et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Structure of the vacuolar-type ATPase from Saccharomyces cerevisiae at 11-Å resolution

(2012) Samir Benlekbir et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Arrangement of subunits in intact mammalian mitochondrial ATP synthase determined by cryo-EM

(2012) L. A. Baker et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Movies of Ice-Embedded Particles Enhance Resolution in Electron Cryo-Microscopy

(2012) Melody G. Campbell et al.   STRUCTURE

Structural Analysis of Macromolecular Assemblies by Electron Microscopy

(2011) E. V. Orlova et al.   CHEMICAL REVIEWS

Crystallizing membrane proteins using lipidic bicelles

(2011) Rachna Ujwal et al.   METHODS

Subnanometre-resolution structure of the intact Thermus thermophilus H+-driven ATP synthase

(2011) Wilson C. Y. Lau et al.   NATURE

Overcoming barriers to membrane protein structure determination

(2011) Roslyn M Bill et al.   NATURE BIOTECHNOLOGY

Validation of the detergent micelle classification for membrane protein crystals and explanation of the Matthews Graph for soluble proteins

(2011) Georg E. Schulz   PROTEIN SCIENCE

Structural divergence of the rotary ATPases

(2011) Stephen P. Muench et al.   QUARTERLY REVIEWS OF BIOPHYSICS

Ryanodine receptor calcium channels and their partners as drug targets

(2010) John J. Mackrill   BIOCHEMICAL PHARMACOLOGY

Making membrane proteins for structures: a trillion tiny tweaks

(2010) Monya Baker   NATURE METHODS

Ryanodine receptor channelopathies

(2010) Matthew J. Betzenhauser et al.   PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY

Structure of intact Thermus thermophilus V-ATPase by cryo-EM reveals organization of the membrane-bound VO motor

(2010) W. C. Y. Lau et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Genome-wide analysis of integral membrane proteins from eubacterial, archaean, and eukaryotic organisms

(2010) Erik Wallin et al.   PROTEIN SCIENCE

Piezo1 and Piezo2 Are Essential Components of Distinct Mechanically Activated Cation Channels

(2010) B. Coste et al.   SCIENCE

Membrane protein assembly into Nanodiscs

(2009) Timothy H. Bayburt et al.   FEBS LETTERS

Cryo-electron Microscopy of the Vacuolar ATPase Motor Reveals its Mechanical and Regulatory Complexity

(2009) Stephen P. Muench et al.   JOURNAL OF MOLECULAR BIOLOGY

Appion: An integrated, database-driven pipeline to facilitate EM image processing

(2009) Gabriel C. Lander et al.   JOURNAL OF STRUCTURAL BIOLOGY

The resolution dependence of optimal exposures in liquid nitrogen temperature electron cryomicroscopy of catalase crystals

(2009) Lindsay A. Baker et al.   JOURNAL OF STRUCTURAL BIOLOGY

Structure of the BK potassium channel in a lipid membrane from electron cryomicroscopy

(2009) Liguo Wang et al.   NATURE

Enhanced imaging in low dose electron microscopy using electron counting

(2009) G. McMullan et al.   ULTRAMICROSCOPY

Coordinated Movement of Cytoplasmic and Transmembrane Domains of RyR1 upon Gating

(2009) Montserrat Samsó et al.   PLOS BIOLOGY

Therapeutic Potential of γ -Secretase Inhibitors and Modulators

(2008) Bruno Imbimbo   CURRENT TOPICS IN MEDICINAL CHEMISTRY

Neurosensory mechanotransduction

(2008) Martin Chalfie   NATURE REVIEWS MOLECULAR CELL BIOLOGY