Phosphatase specificity principles uncovered by MRBLE:Dephos and global substrate identification

An atlas of substrate specificities for the human serine/threonine kinome

(2023) Jared L. Johnson et al.   NATURE

Emerging insights into serine/threonine-specific phosphoprotein phosphatase function and selectivity

(2022) Thomas Kokot et al.   JOURNAL OF CELL SCIENCE

The STRING database in 2023: protein–protein association networks and functional enrichment analyses for any sequenced genome of interest

(2022) Damian Szklarczyk et al.   NUCLEIC ACIDS RESEARCH

Coupling of Cdc20 inhibition and activation by BubR1

(2021) Jamin B. Hein et al.   JOURNAL OF CELL BIOLOGY

Mechanisms of site‐specific dephosphorylation and kinase opposition imposed by PP2A regulatory subunits

(2020) Thomas Kruse et al.   EMBO JOURNAL

Dissecting the sequence determinants for dephosphorylation by the catalytic subunits of phosphatases PP1 and PP2A

(2020) Bernhard Hoermann et al.   Nature Communications

Ordered dephosphorylation initiated by the selective proteolysis of cyclin B drives mitotic exit

(2020) James Holder et al.   eLife

MRBLES 2.0: High-throughput generation of chemically functionalized spectrally and magnetically encoded hydrogel beads using a simple single-layer microfluidic device

(2020) Yinnian Feng et al.   Microsystems & Nanoengineering

An open-source software analysis package for Microspheres with Ratiometric Barcode Lanthanide Encoding (MRBLEs)

(2019) Björn Harink et al.   PLoS One

Quantitative mapping of protein-peptide affinity landscapes using spectrally encoded beads

(2019) Huy Quoc Nguyen et al.   eLife

PP1 and PP2A Use Opposite Phospho-dependencies to Control Distinct Processes at the Kinetochore

(2019) Richard J. Smith et al.   Cell Reports

Aurora B opposes PP1 function in mitosis by phosphorylating the conserved PP1-binding RVxF motif in PP1 regulatory proteins

(2018) Isha Nasa et al.   Science Signaling

Aurora B opposes PP1 function in mitosis by phosphorylating the conserved PP1-binding RVxF motif in PP1 regulatory proteins

(2018) Isha Nasa et al.   Science Signaling

Efficient proximity labeling in living cells and organisms with TurboID

(2018) Tess C Branon et al.   NATURE BIOTECHNOLOGY

KNL1 Binding to PP1 and Microtubules Is Mutually Exclusive

(2018) Rakhi Bajaj et al.   STRUCTURE

Protein phosphatases in the regulation of mitosis

(2018) Jakob Nilsson   JOURNAL OF CELL BIOLOGY

Distinct kinetics of serine and threonine dephosphorylation are essential for mitosis

(2017) Jamin B. Hein et al.   NATURE CELL BIOLOGY

Bub1 positions Mad1 close to KNL1 MELT repeats to promote checkpoint signalling

(2017) Gang Zhang et al.   Nature Communications

A PP2A-B55 recognition signal controls substrate dephosphorylation kinetics during mitotic exit

(2016) Michael J. Cundell et al.   JOURNAL OF CELL BIOLOGY

Identification of Candidate Cyclin-dependent kinase 1 (Cdk1) Substrates in Mitosis by Quantitative Phosphoproteomics

(2016) Adam Petrone et al.   MOLECULAR & CELLULAR PROTEOMICS

The Perseus computational platform for comprehensive analysis of (prote)omics data

(2016) Stefka Tyanova et al.   NATURE METHODS

Programmable Microfluidic Synthesis of Over One Thousand Uniquely Identifiable Spectral Codes

(2016) Huy Q. Nguyen et al.   Advanced Optical Materials

MultiNotch MS3 Enables Accurate, Sensitive, and Multiplexed Detection of Differential Expression across Cancer Cell Line Proteomes

(2014) Graeme C. McAlister et al.   ANALYTICAL CHEMISTRY

Greatwall-phosphorylated Endosulfine is both an inhibitor and a substrate of PP2A-B55 heterotrimers

(2014) Byron C Williams et al.   eLife

Novel Parallelized Quadrupole/Linear Ion Trap/Orbitrap Tribrid Mass Spectrometer Improving Proteome Coverage and Peptide Identification Rates

(2013) Michael W. Senko et al.   ANALYTICAL CHEMISTRY

Cell division: control of the chromosomal passenger complex in time and space

(2013) Armando van der Horst et al.   CHROMOSOMA

The PP1 binding code: a molecular-lego strategy that governs specificity

(2012) Ewald Heroes et al.   FEBS Journal

Programmable microfluidic synthesis of spectrally encoded microspheres

(2012) R. E. Gerver et al.   LAB ON A CHIP

Chemical Visualization of Phosphoproteomes on Membrane

(2012) Anton Iliuk et al.   MOLECULAR & CELLULAR PROTEOMICS

Why nature chose phosphate to modify proteins

(2012) T. Hunter   PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES

Comet: An open-source MS/MS sequence database search tool

(2012) Jimmy K. Eng et al.   PROTEOMICS

Phosphorylation Assay Based on Multifunctionalized Soluble Nanopolymer

(2011) Anton Iliuk et al.   ANALYTICAL CHEMISTRY

PP1/Repo-Man Dephosphorylates Mitotic Histone H3 at T3 and Regulates Chromosomal Aurora B Targeting

(2011) Junbin Qian et al.   CURRENT BIOLOGY

Global quantification of mammalian gene expression control

(2011) Björn Schwanhäusser et al.   NATURE

MS3 eliminates ratio distortion in isobaric multiplexed quantitative proteomics

(2011) Lily Ting et al.   NATURE METHODS

MassChroQ: A versatile tool for mass spectrometry quantification

(2011) Benoît Valot et al.   PROTEOMICS

Quantitative Phosphoproteomics Identifies Substrates and Functional Modules of Aurora and Polo-Like Kinase Activities in Mitotic Cells

(2011) A. N. Kettenbach et al.   Science Signaling

The Substrate of Greatwall Kinase, Arpp19, Controls Mitosis by Inhibiting Protein Phosphatase 2A

(2010) A. Gharbi-Ayachi et al.   SCIENCE

Greatwall Phosphorylates an Inhibitor of Protein Phosphatase 2A That Is Essential for Mitosis

(2010) S. Mochida et al.   SCIENCE

The extended PP1 toolkit: designed to create specificity

(2010) Mathieu Bollen et al.   TRENDS IN BIOCHEMICAL SCIENCES

Serine/Threonine Phosphatases: Mechanism through Structure

(2009) Yigong Shi   CELL

Improved visualization of protein consensus sequences by iceLogo

(2009) Niklaas Colaert et al.   NATURE METHODS

Structure of a Protein Phosphatase 2A Holoenzyme: Insights into B55-Mediated Tau Dephosphorylation

(2008) Yanhui Xu et al.   MOLECULAR CELL