Review
Biochemistry & Molecular Biology
David C. Hughes, Leslie M. Baehr, David S. Waddell, Adam P. Sharples, Sue C. Bodine
Summary: The development and prevalence of diseases associated with aging have a global health burden on society. One hallmark of aging is the loss of proteostasis, partly caused by alterations to the ubiquitin-proteasome system and lysosome-autophagy system, leading to impaired function and maintenance of mass in tissues such as skeletal muscle. In skeletal muscle, functional impairment occurs early in the aging process and depends on proteostatic mechanisms.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
Yawei Ru, Xiaojie Yan, Bing Zhang, Lili Song, Qiqi Feng, Chen Ye, Zhili Zhou, Zhenzhen Yang, Yao Li, Zhenjian Zhang, Qianqian Li, Wenyi Mi, Cheng Dong
Summary: In this study, a new C-degron pathway, called the Gln/C-degron pathway, was discovered. The B30.2 domain of TRIM7 mediates the recognition of proteins with a C-terminal glutamine. The C-terminal residues of the substrate play a crucial role in C-degron recognition.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Biochemistry & Molecular Biology
Masato Mizuno, Shuhei Ebine, Okuto Shounai, Shizuka Nakajima, Shota Tomomatsu, Ken Ikeuchi, Yoshitaka Matsuo, Toshifumi Inada
Summary: This study reveals that polytryptophan sequences can induce Rqc2-independent ribosome-associated quality control (RQC), and this process does not require CAT-tailing. Polytryptophan sequences also induce ribosome-associated quality control coupled with canonical no-go mRNA decay (NGD) and ribosome-associated quality control uncoupled with NGD.
NUCLEIC ACIDS RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Lydia Qian, Laura J. Sharpe, Akira Gokool, Irina Voineagu, Andrew J. Brown
Summary: Circular RNAs (circRNAs) have various functions, and the MARCHF6 circRNA plays a crucial role in cholesterol homeostasis.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS
(2023)
Review
Pharmacology & Pharmacy
Si-Min Qi, Jinyun Dong, Zhi-Yuan Xu, Xiang-Dong Cheng, Wei-Dong Zhang, Jiang-Jiang Qin
Summary: PROTAC technology is an effective method for degrading target proteins through the ubiquitin-proteasome system, with promising applications in cancer treatment. The first oral PROTACs have shown encouraging results in clinical trials, sparking greater enthusiasm for PROTAC research.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Yulemi Gonzalez Quesada, Luc DesGroseillers
Summary: This study investigates the degradation mechanism of RNA-binding protein STAU1 in cancer cells. The research reveals that STAU1 degradation is related to the FPL-motif protein family and inflammation-related protein MAP4K1. Through experiments, it is confirmed that TRIM25 is the E3 ubiquitin ligase responsible for the degradation of STAU1 and MAP4K1. These findings are significant for further exploration of the inflammation mechanisms associated with cancer.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Hyun Mi Kang, Dae Hun Kim, Mijin Kim, Yoohong Min, Bohyeon Jeong, Kyung Hee Noh, Da Yong Lee, Hyun-Soo Cho, Nam-Soon Kim, Cho-Rok Jung, Jung Hwa Lim
Summary: This study discovered that the FBXL17-SPAST pathway is involved in the pathogenicity of hereditary spastic paraplegia (HSP) through loss of function and quantitative regulation. Targeting FBXL17 could provide new insights into HSP therapeutics.
CELL AND BIOSCIENCE
(2022)
Article
Virology
Lei Hou, Xiaohan Hu, Jinshuo Guo, Rong Quan, Li Wei, Jing Wang, Jiangwei Song, Jue Liu
Summary: In this study, we found that aMPV/C infection induces MAVS degradation through the ubiquitin-proteasome pathway at the post-translational level. Furthermore, specific amino acid sites were identified as pivotal in the formation of polyubiquitin chains during MAVS degradation. E3 ubiquitin ligases targeting MAVS were also identified, with RNF5 shown to be involved in MAVS degradation in aMPV/C-infected cells.
Article
Biochemistry & Molecular Biology
Orsolya Bilkei-Gorzo, Tiaan Heunis, Jose Luis Marin-Rubio, Francesca Romana Cianfanelli, Benjamin Bernard Armando Raymond, Joseph Inns, Daniela Fabrikova, Julien Peltier, Fiona Oakley, Ralf Schmid, Anetta Hartlova, Matthias Trost
Summary: This study reveals the importance of phagosomal ubiquitylation and the E3 ubiquitin ligase RNF115 in regulating innate immune functions during bacterial infections.
Article
Chemistry, Medicinal
Adam G. Bond, Conner Craigon, Kwok-Ho Chan, Andrea Testa, Athanasios Karapetsas, Rotimi Fasimoye, Thomas Macartney, J. Julian Blow, Dario R. Alessi, Alessio Ciulli
Summary: This study describes the design and development of a new protein degradation system utilizing a variant of the Brd4 bromodomain as a degradation tag. The system effectively degrades BromoTagged proteins in a fast, selective manner, showing favorable pharmacokinetic profile in mice. This system expands the arsenal of chemical genetic degradation tools for manipulating protein levels and exploring therapeutic potential in cells and in vivo.
JOURNAL OF MEDICINAL CHEMISTRY
(2021)
Article
Cell Biology
Martin P. Schwalm, Lena M. Berger, Maximilian N. Meuter, James D. Vasta, Cesear R. Corona, Sandra Roehm, Benedict-Tilman Berger, Frederic Farges, Sebastian M. Beinert, Franziska Preuss, Viktoria Morasch, Vladimir V. Rogov, Sebastian Mathea, Krishna Saxena, Matthew B. Robers, Susanne Mueller, Stefan Knapp
Summary: E3 ligases play a crucial role in regulating protein homeostasis by recruiting substrate proteins to the proteasomal degradation machinery. Recent research has focused on the Baculovirus IAP Repeat (BIR) family of E3 ligases, which contain a structurally conserved but diverse protein interaction domain. The Inhibitors of Apoptosis (IAP) family, which typically have three BIR domains, are promising drug targets. However, there is currently a lack of assay tools to evaluate the selectivity of inhibitors in this target area.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Biology
Chao Hou, Yuxuan Li, Mengyao Wang, Hong Wu, Tingting Li
Summary: Degpred is a deep learning-based model that predicts general degrons directly from protein sequences. It successfully captures typical degron-related sequence properties and predicts degrons beyond those identified by motif-based methods. Furthermore, it constructs a regulatory network of protein degradation by assigning predicted degrons to specific E3 ubiquitin ligases with calculated motifs.
Review
Biochemistry & Molecular Biology
Na Yang, Bo Kong, Zhaohong Zhu, Fei Huang, Liliang Zhang, Tao Lu, Yadong Chen, Yanmin Zhang, Yulei Jiang
Summary: Targeted protein degradation (TPD) technology, such as PROTACs, has become widespread in the past 20 years and greatly accelerates the development of disease treatment. Unlike small inhibitors, targeted protein degraders can target undruggable targets and overcome drug resistance through ubiquitin-proteasome pathway (UPP) and lysosome pathway. This review discusses different degradation technologies and provides a basis for future research.
MOLECULAR DIVERSITY
(2023)
Review
Biochemistry & Molecular Biology
Rui Zhang, Shaoqing Shi
Summary: HECT-type E3 ubiquitin ligases play a vital role in controlling protein function and stability, and members of the NEDD4 family have critical roles in dysregulation of autophagy in cancer cells. This review focuses on the role of NEDD4 E3 ligases in defective autophagy in cancer cells, discussing their function, substrates, and signaling pathways, providing a basis for cancer treatment through modulation of these ligases.
MOLECULAR MEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Bayonle Aminu, Julia Fux, Evan Mallette, Nathaniel Petersen, Wei Zhang
Summary: The Ubiquitin Variant Induced Proximity (UbVIP) technology allows for targeted degradation of proteins, showing potential as a therapeutic approach for human diseases. By utilizing synthetic UbV binders and designing multi-domain fusion proteins, researchers were able to induce proteasomal degradation of a nucleus-localized protein 53BP1. This study demonstrates the suitability of UbV technology for developing protein-based molecules for targeted degradation and identifying novel E3 ligases.
Article
Oncology
Jeong A. Bae, Dhong Hyo Kho, Eun Gene Sun, Yoo-Seung Ko, Somy Yoon, Kyung Hwa Lee, Kyu Youn Ahn, Jae Hyuk Lee, Young Eun Joo, Ik Joo Chung, Sug Hyung Lee, Hangun Kim, Kyung Keun Kim
CLINICAL CANCER RESEARCH
(2016)
Article
Medicine, General & Internal
Moon-Soo Han, Yeong-Jin Kim, Kyung-Sub Moon, Kyung-Hwa Lee, Jung-In Yang, Woo Dae Kang, Sa-Hoe Lim, Woo-Youl Jang, Tae-Young Jung, In-Young Kim, Shin Jung
Article
Biochemistry & Molecular Biology
So-Yeon Park, Somy Yoon, Eun Gene Sun, Rui Zhou, Jeong A. Bae, Young-Woo Seo, Jung-Il Chae, Man-Jeong Paik, Hyung-Ho Ha, Hangun Kim, Kyung Keun Kim
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2017)
Article
Biochemistry & Molecular Biology
Sam-Young Park, Eun-Gene Sun, Yeonju Lee, Min-Seok Kim, Jae-Hyung Kim, Won-Jae Kim, Ji-Yeon Jung
JOURNAL OF CELLULAR BIOCHEMISTRY
(2018)
Article
Medicine, General & Internal
Nah-Ihm Kim, Jung-In Yang, Sung-Sun Kim, Ji-Shin Lee, Sang-Chul Lim, Shin Jung, Jae-Hyuk Lee, Kyung-Sub Moon, Kyung-Hwa Lee
Article
Oncology
Woo-Kyun Bae, Chang-Soo Hong, Mi-Ra Park, Eun-Gene Sun, Ji-Hee Lee, Keunsoo Kang, Kyung-Hyun Ryu, Hyun-Jeong Shim, Jun-Eul Hwang, Sang-Hee Cho, Ik-Joo Chung
Article
Multidisciplinary Sciences
Yi Yang, So-Yeon Park, Thanh Thi Nguyen, Young Hyun Yu, Tru Van Nguyen, Eun Gene Sun, Jayalal Udeni, Min-Hye Jeong, Iris Pereira, Cheol Moon, Hyung-Ho Ha, Kyung Keun Kim, Jae-Seoun Hur, Hangun Kim
Article
Cell Biology
Youn-Sang Jung, Sohee Jun, Moon Jong Kim, Sung Ho Lee, Han Na Suh, Esther M. Lien, Hae-Yun Jung, Sunhye Lee, Jie Zhang, Jung-In Yang, Hong Ji, Ji Yuan Wu, Wenqi Wang, Rachel K. Miller, Junjie Chen, Pierre D. McCrea, Scott Kopetz, Jae-Il Park
NATURE CELL BIOLOGY
(2018)
Article
Immunology
Chang-Soo Hong, Mi-Ra Park, Eun-Gene Sun, Wonyoung Choi, Jun-Eul Hwang, Woo-Kyun Bae, Joon Haeng Rhee, Sang-Hee Cho, Ik-Joo Chung
FRONTIERS IN IMMUNOLOGY
(2019)
Article
Biochemistry & Molecular Biology
Yoo-Seung Ko, Jeong A. Bae, Keon Young Kim, Sung Jin Kim, Eun Gene Sun, Kyung Hwa Lee, Nacksung Kim, Hyuno Kang, Young-Woo Seo, Hangun Kim, Ik Joo Chung, Kyung Keun Kim
Article
Oncology
Chang-Soo Hong, Eun-Gene Sun, Ji-Na Choi, Dae-Hwan Kim, Jo-Heon Kim, Kyung-Hyun Ryu, Hyun-Jeong Shim, Jun-Eul Hwang, Woo-Kyun Bae, Hyeong-Rok Kim, Kyung Keun Kim, Chaeyong Jung, Ik-Joo Chung, Sang-Hee Cho
Article
Multidisciplinary Sciences
Ritesh K. Aggarwal, Rebecca A. Luchtel, Venkata Machha, Alexander Tischer, Yiyu Zou, Kith Pradhan, Nadia Ashai, Nandini Ramachandra, Joseph M. Albanese, Jung-in Yang, Xiaoyang Wang, Srinivas Aluri, Shanisha Gordon, Ahmed Aboumohamed, Benjamin A. Gartrell, Sassan Hafizi, James Pullman, Niraj Shenoy
Summary: Reduced succinate dehydrogenase (SDH) activity leading to oncogenic succinate accumulation is a common feature in clear cell renal cell carcinoma (ccRCC), with significant adverse impact on patient survival. This study highlights the importance of SDH deficiency in ccRCC pathogenesis and progression.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Oncology
Se-Jeong Oh, Jung-In Yang, Ok Kim, Eun-Jung Ahn, Woo Dae Kang, Jae-Hyuk Lee, Kyung-Sub Moon, Kyung-Hwa Lee, Duck Cho
CANCER CELL INTERNATIONAL
(2017)