Article
Infectious Diseases
Taehyeong Lim, So-Young Ham, SangJin Nam, Myoungsun Kim, Ki Yong Lee, Hee-Deung Park, Youngjoo Byun
Summary: This article reviews recent studies on small molecules targeting the RhlI/RhlR system in P. aeruginosa, discussing their in vitro and in vivo biological activities. The article focuses on structure-activity relationship studies and the outlook for next-generation anti-biofilm agents.
Article
Biotechnology & Applied Microbiology
James Redfern, Janine Wallace, Alex van Belkum, Magali Jaillard, Elliot Whittard, Roobinidevi Ragupathy, Joanna Verran, Peter Kelly, Mark Charles Enright
Summary: Pseudomonas aeruginosa is a common environmental microorganism that can form biofilms on indwelling device surfaces, promoting adhesion and survival. Research identified accessory genes and core-genome SNPs associated with enhanced biofilm production at 22 degrees C, including a 165 kb genomic island with heavy metal resistance genes.
Article
Infectious Diseases
Tomasz Bogiel, Dagmara Depka, Mateusz Rzepka, Joanna Kwiecinska-Pirog, Eugenia Gospodarek-Komkowska
Summary: The study investigated the frequency and genotype distribution of 10 virulence factor genes in 107 non-duplicated carbapenem-resistant P. aeruginosa isolates. Variations were observed in the prevalence and distribution of virulence genes, especially in terms of their carbapenemase synthesis ability and the strain origin.
Article
Biology
Steffen Geisel, Eleonora Secchi, Jan Vermant
Summary: Biofilms are bacterial communities encased by a self-produced matrix, and the channel networks formed within the biofilm play a vital role in promoting the transport of nutrients and chemicals. This study focuses on the role of the biofilm matrix adhesion in channel formation, specifically in Pseudomonas aeruginosa biofilms grown under constant flow in microfluidic channels. The formation of wrinkles and folds is triggered by a mechanical buckling instability, resulting in hollow channels that facilitate bacterial movement.
Review
Biotechnology & Applied Microbiology
Anming Xu, Xiaoxiao Zhang, Tong Wang, Fengxue Xin, Luyan Z. Ma, Jie Zhou, Weiliang Dong, Min Jiang
Summary: Pseudomonas aeruginosa is known for its excellent ecological flexibility and can survive in diverse niches. The adaptive mutants RSCVs have been frequently isolated from chronic infections, sparking interest in investigating their formation, persistence, and pathogenesis. RSCVs are believed to play a significant role in biofilm formation and high adaptability.
BIOTECHNOLOGY ADVANCES
(2021)
Article
Chemistry, Multidisciplinary
Xiaodie Yuan, Xia Zhao, Yuan Lin, Zhaohui Su
Summary: This study synthesized PDA nanoparticles of various sizes and introduced different surface groups to investigate their penetration and accumulation abilities in biofilms. The PDA nanoparticles of approximately 60 nm size showed higher penetration and accumulation abilities, and the positively charged amine groups were more effective in enhancing the interactions with the biofilm compared to carboxyl or methoxy groups. The PDA60 nanoparticles with amine surface groups also exhibited good photothermal properties.
Article
Infectious Diseases
Emilie Charpentier, Ludovic Doudet, Ingrid Allart-Simon, Marius Colin, Sophie C. Gangloff, Stephane Gerard, Fany Reffuveille
Summary: The combination of non-antimicrobial molecules with antibiotics can significantly reduce bacterial biofilm formation, potentially offering a solution to combat antibiotic resistance emergence.
Article
Pharmacology & Pharmacy
Davoodbasha MubarakAli, Kannappan Arunachalam, Murugan Lakshmanan, Bazigha Badar, Jung-Wan Kim, Sang-Yul Lee
Summary: Biofilm-related nosocomial infections can cause various life-threatening infections. In this study, hydroxyapatite nanoparticles (HANPs) were synthesized using a co-precipitation method followed by microwave treatment, and their ability to inhibit the biofilm formation of Pseudomonas aeruginosa ATCC 10145 was investigated. The results showed that the synthesized HANPs effectively inhibited biofilm formation, suggesting their potential use in controlling biofilm-related infections caused by Pseudomonas aeruginosa.
Article
Immunology
Zuoji Ye, Liumei Ye, Dingbin Li, Shunsheng Lin, Wusheng Deng, Li Zhang, Jinhua Liang, Jinlong Li, Qingjun Wei, Ke Wang
Summary: In this study, the researchers found that Daphnetin (DAP) can effectively inhibit and eradicate biofilm formation of Pseudomonas aeruginosa. DAP also reduced bacterial motility and pyocyanin production. These findings suggest that DAP may serve as a natural anti-biofilm therapeutic agent.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Christian Schuetz, Duy-Khiet Ho, Mostafa Mohamed Hamed, Ahmed Saad Abdelsamie, Teresa Roehrig, Christian Herr, Andreas Martin Kany, Katharina Rox, Stefan Schmelz, Lorenz Siebenbuerger, Marius Wirth, Carsten Boerger, Samir Yahiaoui, Robert Bals, Andrea Scrima, Wulf Blankenfeldt, Justus Constantin Horstmann, Rebekka Christmann, Xabier Murgia, Marcus Koch, Aylin Berwanger, Brigitta Loretz, Anna Katharina Herta Hirsch, Rolf Wolfgang Hartmann, Claus-Michael Lehr, Martin Empting
Summary: A new generation of quorum sensing inhibitors (QSI) is reported to effectively inhibit the virulence of Pseudomonas aeruginosa and enhance antibiotic efficacy, potentially preventing the development of antimicrobial resistance. Additionally, a significant synergistic effect of QSI in combination with tobramycin against P. aeruginosa biofilms is demonstrated, showing promising implications for adjunctive pathoblocker-mediated therapy.
Article
Chemistry, Multidisciplinary
Pengyu Li, Jieyi Pan, Yating Dong, Yingying Sun, Yalong Wang, Kang Liao, Yili Chen, Xin Deng, Shihui Yu, Haiyan Hu
Summary: Chronic pulmonary infection caused by Pseudomonas aeruginosa is a serious public health problem with high mortality rates. In this study, infection-microenvironment responsive nanoparticles were developed to eradicate biofilms and inhibit virulence. These nanoparticles showed promising results in treating chronic pulmonary infections.
JOURNAL OF CONTROLLED RELEASE
(2024)
Article
Immunology
Qi Tan, Qing Ai, Yu He, Fang Li, Jialin Yu
Summary: The ability of P.aeruginosa biofilms to activate inflammasomes and increase inflammatory response contributes to chronic infection. This study found that P.aeruginosa biofilms significantly enhance the production of IL-1β and IL-18, possibly through the regulation of NLRP3 inflammasomes rather than NLRC4 inflammasomes.
MICROBIAL PATHOGENESIS
(2022)
Article
Immunology
Laia Fernandez-Barat, Nil Vazquez Burgos, Victoria Alcaraz, Leticia Bueno-Freire, Ruben Lopez-Aladid, Roberto Cabrera, Albert Gabarrus, Andrea Palomeque, Patricia Oscanoa, Adrian Ceccato, Ana Motos, Rosanel Amaro, Thierry Bernardi, Christian Provot, Alba Soler-Comas, Laura Munoz, Jordi Vila, Antoni Torres
Summary: The aim of this study was to validate and identify additional applications of the BioFilm Ring Test (BRT) for Pseudomonas aeruginosa (PA) isolates from patients with bronchiectasis (BE), and evaluate its relationship with antimicrobial stewardship. The results showed that a BPI value below 14.75 at 5 hours predicted the mucoid PA phenotype. In conclusion, the BRT has the potential to reveal biofilm features with clinical implications.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Microbiology
Hong Chen, Peng-Cheng Ji, Yue-Heng Qi, Shi-Jin Chen, Chang-Yao Wang, Yu-Jie Yang, Xin-Yu Zhao, Jin-Wei Zhou
Summary: This study evaluated the synergistic effect of thymoquinone and nisin in reducing biofilm formation of P. aeruginosa on lettuce, and investigated their anti-virulence and anti-biofilm mechanisms. Thymoquinone inhibited the production of autoinducers and virulence factors, and enhanced the susceptibility of P. aeruginosa biofilms to nisin. It disrupted the quorum sensing system, altered cell membrane component, and down-regulated the expressions of genes related to virulence, efflux pump, and antioxidation.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Biophysics
Wing-Hin Lee, Ramin Rohanizadeh, Ching-Yee Loo
Summary: This study developed a novel bioactive bone substitute with improved anti-biofilm activity by functionalizing with curcumin, which effectively inhibits biofilm formation and controls viable planktonic cells. Curcumin was successfully incorporated onto HA and sustainably released over 14 days, showing stronger anti-biofilm effect against Staphylococcus aureus. The curcumin-loaded HA composite could be an alternative antimicrobial agent to control the risk of infections in postsurgical implants.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2021)
Article
Chemistry, Medicinal
Monika K. Lesiow, Piotr Pietrzyk, Agnieszka Kyziol, Urszula K. Komamicka
CHEMICAL RESEARCH IN TOXICOLOGY
(2019)
Article
Biochemistry & Molecular Biology
Justyna Jaszczyk, Sizhe Li, Chloe Cocaud, Cyril Nicolas, Olivier R. Martin
CARBOHYDRATE RESEARCH
(2019)
Article
Biochemistry & Molecular Biology
Urszula K. Komarnicka, Sandra Koziel, Piotr Zabierowski, Rafal Kruszynski, Monika K. Lesiow, Francesco Tisato, Marina Porchia, Agnieszka Kyziol
JOURNAL OF INORGANIC BIOCHEMISTRY
(2020)
Review
Engineering, Environmental
Agnieszka Kyziol, Wahid Khan, Victor Sebastian, Karol Kyziol
CHEMICAL ENGINEERING JOURNAL
(2020)
Article
Chemistry, Applied
Sizhe Li, Justyna Jaszczyk, Xavier Pannecoucke, Thomas Poisson, Olivier R. Martin, Cyril Nicolas
Summary: A process for synthesizing imino-C-glycosides is described, using 1-C-stannylated iminosugars and various electrophiles in Migita-Kosugi-Stille cross-couplings. The methodology allows for stereoretentive synthesis of 1-C-substituted iminosugar derivatives, with controllable configuration and tunable outcomes. This approach enables the synthesis of pseudo anomers of imino-C-glycosyl compounds in a controlled manner through a stereoretentive C-C bond-forming process.
ADVANCED SYNTHESIS & CATALYSIS
(2021)
Article
Chemistry, Organic
Isabelle Gillaizeau, Ismael Dondasse, Cyril Nicolas, Liliane Mimoun, Volodymyr Sukach, Herve Meudal
Summary: An efficient method for the catalytic preparation of new beta-borylated enamides was achieved through regioselective iridium-catalyzed C(sp(2))-H borylation under relatively mild conditions. This method demonstrates broad substrate scope, good functional group tolerance, and efficient scale-up. The resulting C-3 borylated enamides can be advantageously utilized in cross-coupling reactions and rapidly converted into valuable 3,3-dihalogeno-2-methoxypiperidines.
EUROPEAN JOURNAL OF ORGANIC CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Anna Wadolek, Dominika Drwila, Maria Oszajca, Grazyna Stochel, Ewa Konduracka, Malgorzata Brindell
Summary: Long-term exposure to air pollutants can increase mortality and deteriorate life parameters, especially among those with cardiovascular diseases or impaired respiratory systems. Studies have shown that transition metal ions in airborne particulate matter can disrupt the stability of S-nitrosothiols in the blood plasma, affecting the balance of nitric oxide.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Organic
Baptiste Taffoureau, Isabelle Gillaizeau, Pascal Retailleau, Cyril Nicolas
Summary: In this study, the synthesis and crystallization of 2,3,5-tri-O-benzyl-D-xylofuranose led to the isolation of the alpha anomer, with a small contamination of the beta form. The crystallographic structure of the alpha anomer was determined at 100 K and refined using a Hirshfeld atom refinement approach.
Article
Chemistry, Organic
Olivier R. Martin, Cyril Nicolas, Tuniyazi Abuduaini, Sizhe Li, Vincent Roy, Luigi A. Agrofoglio
Summary: A new methodology for the synthesis of biologically interesting compounds was developed, allowing the preparation of unknown and complex sugars in a stereospecific manner.
JOURNAL OF ORGANIC CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Nicolas G. Biteau, Vincent Roy, Cyril Nicolas, Hubert F. Becker, Jean-Christophe Lambry, Hannu Myllykallio, Luigi A. Agrofoglio
Summary: The newly discovered FDTS is considered an attractive target for antibacterial agents against multi-resistant pathogens. A novel series of pyrido[1,2-e]purine-2,4(1H,3H)-dione analogues were synthesized and their structure-activity relationships studied, with compound 23a showing the highest inhibition.
Article
Biochemistry & Molecular Biology
Magdalena Ziabka, Katarzyna Matysiak, Katarzyna Cholewa-Kowalska, Agnieszka Kyziol, Aleksandra Krolicka, Rafal Sapierzynski, Monika Januchta-Kurmin, Igor Bissenik
Summary: The aim of this study was to evaluate the biological properties of hybrid coatings modified with Ag, Cu, and Zn nanoparticles (NPs) applied on TPLO medical implants. The coatings enriched with metallic NPs were tested for bactericidal efficacy against bacteria and yeast, as well as on human osteosarcoma cell lines. The results showed that AgNPs had the strongest bactericidal effect, but higher concentrations of metallic additives had a toxic effect. In vivo tests on canine patients showed no complications, and the low dose of AgNPs facilitated tissue healing.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Organic
Daniel Kamzol, Maxime Neuville, Chloe Cocaud, Killian Tiger, Baptiste Taffoureau, Krzysztof Lewinski, Justyna Jaszczyk, Olivier R. Martin, Sebastian Bas, Sylvain Routier, Isabelle Gillaizeau, Frederic Buron, Cyril Nicolas
Summary: This study comprehensively investigates the preparation and reactivity of N-tert-butanesulfinyl glycosylamines with simple Grignard and organo lithium reagents in batch and continuous flow chemistry. These carbohydrate derivatives, acting as latent imine equivalents, react readily with various carbon nucleophiles, making them useful precursors for the diastereoselective synthesis of bioactive compounds such as iminosugar-C-glycosides. Additionally, a hybrid protocol combining flow addition of benzylmagnesium chloride to a (S-R)-arabinofuranosylamine substrate at room temperature with a batch cyclization protocol is introduced for the first time. Notably, this semi-continuous flow process shortens the synthesis of imino-C-glycoside scaffolds to a single workday.
EUROPEAN JOURNAL OF ORGANIC CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Sylvain Bertho, Radhouan Maazaoui, Damla Torun, Ismael Dondasse, Raoudha Abderrahim, Cyril Nicolas, Isabelle Gillaizeau
Summary: An attractive and cost-effective alternative approach using FeCl2 or non-toxic iron powder was developed for the beta-C(sp(2))-H (fluoro)alkylation of a range of cyclic and acyclic non-aromatic enamides, showing regioselectivity, broad substrate scope, and good functional group tolerance.
NEW JOURNAL OF CHEMISTRY
(2021)
Article
Chemistry, Organic
Yoshinori Tokairin, Hiroyuki Konno, Angeline Noireau, Caroline West, Hiroki Moriwaki, Vadim A. Soloshonok, Cyril Nicolas, Isabelle Gillaizeau
Summary: A new approach was developed for the asymmetric synthesis of beta-phosphorus-containing alpha-amino acids by utilizing chiral Ni(ii) complexes to catalyze the Michael addition of dehydroalanine-Schiff base with phosphine oxides and dialkyl phosphites.
ORGANIC CHEMISTRY FRONTIERS
(2021)
Article
Biochemistry & Molecular Biology
Monika Katarzyna Lesiow, Urszula Katarzyna Komarnicka, Agnieszka Kyziol, Alina Bienko, Piotr Pietrzyk