Use of sodium alginate coatings to improve bioavailability of liposomes containing DPP-IV inhibitory collagen peptides

Sodium alginate (SA) was used to coat liposomes containing DPP-IV inhibitory collagen peptides to improve their stability and in vitro absorption for intra-oral delivery. The liposome structure as well as entrapment efficiency and DPP-IV inhibitory activity was characterized. The liposome stability was determined by measuring in vitro release rates and their gastrointestinal stability. Transcellular permeability of liposomes was further tested to characterize their permeability in small intestinal epithelial cells. The results showed that the 0.3% SA coating increased the diameter (166.7 nm to 249.9 nm), absolute value of zeta potential (30.2 mV to 40.1 mV) and entrapment efficiency (61.52% to 70.99%) of liposomes. The SA-coated liposomes containing collagen peptides showed enhanced storage stability within one month, gastrointestinal stability increased by 50% in bioavail-ability, transcellular permeability increased by 18% in transmission percentage, and in vitro release rates reduced by 34%, compared to uncoated liposomes. SA coating liposomes are promising carriers for transporting hy-drophilic molecules, may be beneficial for improving nutrient absorption and can protect bioactive compounds from being inactivated in the gastrointestinal tract.

Automated summary

Sodium alginate (SA) was utilized to improve the stability and in vitro absorption of liposomes containing DPP-IV inhibitory collagen peptides for intra-oral delivery. The SA coating increased the diameter, zeta potential, and entrapment efficiency of liposomes. The coated liposomes exhibited enhanced storage stability, gastrointestinal stability, transcellular permeability, and reduced in vitro release rates compared to uncoated liposomes. SA coated liposomes show potential as carriers for hydrophilic molecules, improving nutrient absorption, and protecting bioactive compounds.