Unexpected Mechanism and Inhibition Effect for Nonspecific Amplification Involving Dynamic Binding of Primers with Background DNA

Thermodynamic evaluation of the impact of DNA mismatches in PCR-type SARS-CoV-2 primers and probes

(2021) Pâmella Miranda et al.   MOLECULAR AND CELLULAR PROBES

Inhibition of nonspecific polymerase activity using Poly(Aspartic) acid as a model anionic polyelectrolyte

(2021) Assol R. Sakhabutdinova et al.   ANALYTICAL BIOCHEMISTRY

Nonspecific Synthesis in the Reactions of Isothermal Nucleic Acid Amplification

(2021) Nadezhda V. Zyrina et al.   BIOCHEMISTRY-MOSCOW

CRISPR-Cas12a based internal negative control for nonspecific products of exponential rolling circle amplification

(2020) Bo Tian et al.   NUCLEIC ACIDS RESEARCH

Real-time kinetics and high-resolution melt curves in single-molecule digital LAMP to differentiate and study specific and non-specific amplification

(2020) Justin C Rolando et al.   NUCLEIC ACIDS RESEARCH

Robust storage of Chinese language in a pool of small single-stranded DNA rings and its facile reading-out

(2020) Chenru Wang et al.   BULLETIN OF THE CHEMICAL SOCIETY OF JAPAN

The influence of quality of primers on the formation of primer dimers in PCR

(2020) Ravil R. Garafutdinov et al.   NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS

High-Fidelity Nanopore Sequencing of Ultra-Short DNA Targets

(2019) Brandon D. Wilson et al.   ANALYTICAL CHEMISTRY

Impact of primer dimers and self-amplifying hairpins on reverse transcription loop-mediated isothermal amplification detection of viral RNA

(2018) Robert J. Meagher et al.   ANALYST

Reduction of Background Generated from Template-Template Hybridizations in the Exponential Amplification Reaction

(2018) Michael S. Reid et al.   ANALYTICAL CHEMISTRY

Improved PCR flexibility with Hot Start dNTPs

(2018) Tony Le et al.   BIOTECHNIQUES

Improving nanopore read accuracy with the R2C2 method enables the sequencing of highly multiplexed full-length single-cell cDNA

(2018) Roger Volden et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Highly Sensitive Detection of Low-Abundance White Spot Syndrome Virus by a Pre-Amplification PCR Method

(2017) Xiaoming Pan et al.   JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY

Zero-Background Helicase-Dependent Amplification and Its Application to Reliable Assay of Telomerase Activity in Cancer Cell by Eliminating Primer-Dimer Artifacts

(2016) Feng Chen et al.   CHEMBIOCHEM

High-throughput thermal stability assessment of DNA hairpins based on high resolution melting

(2016) Jing Wang et al.   JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS

INC-Seq: accurate single molecule reads using nanopore sequencing

(2016) Chenhao Li et al.   GigaScience

Highly specific DNA detection from massive background nucleic acids based on rolling circle amplification of target dsDNA

(2014) Xingyu Wang et al.   RSC Advances

Cooperative Primers

(2013) Brent C. Satterfield   JOURNAL OF MOLECULAR DIAGNOSTICS

Single molecule quantitation and sequencing of rare translocations using microfluidic nested digital PCR

(2013) Joe Shuga et al.   NUCLEIC ACIDS RESEARCH

Comparison of Next-Generation Sequencing Systems

(2012) Lin Liu et al.   JOURNAL OF BIOMEDICINE AND BIOTECHNOLOGY

RNase H-dependent PCR (rhPCR): improved specificity and single nucleotide polymorphism detection using blocked cleavable primers

(2011) Joseph R Dobosy et al.   BMC BIOTECHNOLOGY

Polymerase-Endonuclease Amplification Reaction (PEAR) for Large-Scale Enzymatic Production of Antisense Oligonucleotides

(2010) Xiaolong Wang et al.   PLoS One

Improved PCR specificity with Hot Start PCR primers

(2009) Elena Hidalgo Ashrafi et al.   BIOTECHNIQUES

Specific versus Nonspecific Isothermal DNA Amplification through Thermophilic Polymerase and Nicking Enzyme Activities†

(2008) Eric Tan et al.   BIOCHEMISTRY