Article
Medicine, General & Internal
Menglong Xu, Gaoxin Lei, Manman Chen, Ke Wang, Wenxiu Lv, Panpan Zhang, Tuo Hu, Jie Gao, Chenchen Lu, Ying Mei, Zhipan Xu, Zhengli Bai, Huajing Hu, Yiwei Jiang, Shuhua Tan
Summary: A fully human antibody FAP2M21 with potent inhibitory effect on PCSK9 was generated, showing promise as a therapeutic agent for treating hypercholesterolemia and associated cardiovascular diseases.
Article
Medicine, Research & Experimental
Yang Qin, Jiayi Jin, Jiani Zhang, Hui Wang, Li Liu, Yanwen Zhang, Sunwang Ling, Jinzhu Hu, Nuan Li, Jianguang Wang, Chen Lv, Xinyu Yang
Summary: Rheumatoid arthritis (RA) is a common chronic autoimmune disease for which traditional treatments often fall short. In this study, researchers developed a fully human SEMA5A-blocking antibody and demonstrated its effectiveness in inhibiting disease progression and improving disease characteristics in a mouse model of RA.
BIOMEDICINE & PHARMACOTHERAPY
(2023)
Review
Biochemistry & Molecular Biology
Seyedeh Zahra Bahojb Mahdavi, Fatemeh Oroojalian, Shirin Eyvazi, Maryam Hejazi, Behzad Baradaran, Nasser Pouladi, Mohammad Reza Tohidkia, Ahad Mokhtarzadeh, Serge Muyldermans
Summary: Antibodies, as therapeutic and diagnostic molecules, play a crucial role in disease treatment, especially in cancer therapy. Alternative approaches, such as display selection technologies, have been developed to overcome the limitations of traditional hybridoma technology. These methods, including phage display, bacterial display, and yeast display, enable the screening and generation of high affinity antibodies with low immunogenicity risk. Understanding the basics of each method is important for antibody design suitable for different diseases, including cancer.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Biochemistry & Molecular Biology
Mohammad Ridhuan Mohd Ali, Jia Siang Sum, Nurul Najian Aminuddin Baki, Yee Siew Choong, Nur Asyura Nor Amdan, Fairuz Amran, Theam Soon Lim
Summary: This study aimed to develop specific monoclonal antibodies against LipL21 antigen for future use in rapid and accurate immunoassay for leptospirosis. The generated monoclonal antibody against rLipL21 will be evaluated as a detection reagent for the diagnosis of human leptospirosis in a future study.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Biochemistry & Molecular Biology
Hye Lim Choi, Ha Rim Yang, Ha Gyeong Shin, Kyusang Hwang, Ji Woong Kim, Ji Hyun Lee, Taehoon Ryu, Yushin Jung, Sukmook Lee
Summary: Antibody phage display is a crucial technology for discovering and developing target-specific monoclonal antibodies. This study constructed a large human combinatorial single-chain variable fragment library with high diversity, which can be used for the rapid development of recombinant human monoclonal antibodies for therapeutic and diagnostic applications.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemical Research Methods
Wen-Ching Lin, Hao-Cheng Tang, Han Ying Wang, Chia-Yi Kao, You-Chiun Chang, Athena Hsu Li, Shi-Bing Yang, Kurt Yun Mou
Summary: This study introduces a unique mutagenesis method called the reverse Kunkel method, which combines the advantages of user-defined mutagenesis and random mutagenesis. By coupling with phage display and yeast display selections, the method successfully generates improved antibodies with enhanced affinity and immunostaining performance.
ACS SYNTHETIC BIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Jingru Liang, Hang Dong, Fei Xu, Baowei Li, Haimei Li, Limei Chen, Mei Li, Yingchu Liu, Guosheng Jiang, Jinhua Dong
Summary: In this study, a monoclonal antibody named ESC9 was developed, and an immunosensor called ESC9 Quenchbody (Q-body) was generated for rapid and sensitive detection of estradiol. The ESC9 Q-body showed potential for clinical applications due to its excellent detection speed and sensitivity.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Yu Jung Kim, Min Ho Lee, Se-Ra Lee, Hyo-Young Chung, Kwangmin Kim, Tae Gyu Lee, Dae Young Kim
Summary: Phage display was used to develop human monoclonal antibodies (mAbs) that neutralize SARS-CoV-2. These antibodies showed desirable neutralizing activities and properties, making them potential candidates for antibody therapeutics for treating COVID-19, as well as for diagnostics and research tools.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Nora Langreder, Dorina Schaeckermann, Doris Meier, Marlies Becker, Maren Schubert, Stefan Duebel, Thomas Reinard, Stefanie Figge-Wegener, Kristine Rossbach, Wolfgang Baeumer, Simone Ladel, Michael Hust
Summary: Insect bite hypersensitivity (IBH), the most common allergic skin disease in horses, is caused by bites from Culicoides spp. insects, resulting in a type I/IVb allergy involving eosinophil cells. A potential treatment option is the use of a therapeutic antibody targeting equine interleukin 5, the main activator and regulator of eosinophils. Through phage display and in vitro affinity maturation, an antibody named NOL226-2-D10 was developed, showing strong inhibition of interleukin 5 binding and satisfactory producibility. This antibody is a promising candidate for in vivo studies to treat equine IBH.
SCIENTIFIC REPORTS
(2023)
Article
Medicine, Research & Experimental
Romain Rouet, Ohan Mazigi, Gregory J. Walker, David B. Langley, Meghna Sobti, Peter Schofield, Helen Lenthall, Jennifer Jackson, Stephanie Ubiparipovic, Jake Y. Henry, Arunasingam Abayasingam, Deborah Burnett, Anthony Kelleher, Robert Brink, Rowena A. Bull, Stuart Turville, Alastair G. Stewart, Christopher C. Goodnow, William D. Rawlinson, Daniel Christ
Summary: This study investigates how to alter and broaden the specificity of existing antibodies to effectively neutralize SARS-CoV-2. The research shows that while some clones maintain the specificity of the parental antibody, new specificities also emerge, leading to the development of variants targeting diverse epitopes when paired with a diverse VL repertoire.
Article
Immunology
Brian M. Petersen, Sophia A. Ulmer, Emily R. Rhodes, Matias F. Gutierrez-Gonzalez, Brandon J. Dekosky, Kayla G. Sprenger, Timothy A. Whitehead
Summary: Monoclonal antibodies are key therapeutics for cancer, inflammation, and infectious diseases. Natural human antibody repertoire-based scoring matrices can accurately predict mutations in therapeutic antibodies. High frequency mutations in natural human antibody repertoires have the potential to improve existing therapeutic antibodies and reduce immunogenicity.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Fisheries
Karen Manoutcharian, Goar Gevorkian
Summary: The development of recombinant antibody fragments as alternatives to full-length immunoglobulins offers great potential in biomedicine. These antibody fragments have numerous advantages over conventional monoclonal antibodies, such as improved stability and solubility, reduced immunogenicity, higher specificity and affinity, and the ability to target hidden epitopes and cross the blood-brain barrier. Different formats of antibody fragments, like Fab, scFv, sdAbs, and bsAbs, are being evaluated for diagnostics and therapeutics in various studies. This review specifically focuses on VNARs, the smallest recombinant antibody fragment obtained through phage display antibody libraries, and discusses the relevant research up until March 2023.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Yang Qin, Gan Wu, Jiayi Jin, Hao Wang, Jiani Zhang, Li Liu, Heping Zhao, Jianguang Wang, Xinyu Yang
Summary: A fully human CTGF blocking monoclonal antibody was generated using phage display technology. The antibody exhibited high affinity to CTGF and effectively alleviated arthritis and inhibited angiogenesis in CIA mice.
Article
Biochemistry & Molecular Biology
Omar U. Guzman-Bringas, Keyla M. Gomez-Castellano, Edith Gonzalez-Gonzalez, Juana Salinas-Trujano, Said Vazquez-Leyva, Luis Vallejo-Castillo, Sonia M. Perez-Tapia, Juan C. Almagro
Summary: We isolated and characterized anti-SARS-CoV-2 antibodies from a convalescent COVID-19 patient's VH repertoire, combined with four synthetic VL libraries. One of the antibodies, IgG-A7, showed neutralizing activity against the Wuhan, Delta, and Omicron strains, and protected transgenic mice from SARS-CoV-2 infection. General-purpose libraries, in combination with affinity optimization methods, can provide potent neutralizing antibodies, which is especially important for rapidly evolving viruses like SARS-CoV-2.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Medicine, Research & Experimental
Andre Azevedo Reis Teixeira, Michael Frank Erasmus, Sara D'Angelo, Leslie Naranjo, Fortunato Ferrara, Camila Leal-Lopes, Oliver Durrant, Cecile Galmiche, Aleardo Morelli, Anthony Scott-Tucker, Andrew Raymon Morton Bradbury
Summary: This paper introduces a new antibody library format that generates high-affinity binders with drug-like developability properties directly from initial selections, reducing the need for further engineering or affinity maturation. This innovative design is expected to accelerate drug development processes by reducing the failure rate of leads due to poor antibody affinities and developability.
Article
Oncology
Eun-Hee Nam, Yunhee Lee, Byul Moon, Jung Weon Lee, Semi Kim
Article
Oncology
Yunhee Lee, Dongjoon Ko, Hye-Jin Min, Sol Bi Kim, Hye-Mi Ahn, Younghoon Lee, Semi Kim
Article
Biochemistry & Molecular Biology
Ji Ae Kim, Da Kyung Choi, Jung Sun Min, Inho Kang, Jin Chul Kim, Semi Kim, Jeong Keun Ahn
Article
Biochemistry & Molecular Biology
Heegwon Shin, Jungmin Lee, Youngmi Kim, Seonghui Jang, Yunhee Lee, Semi Kim, Younghoon Lee
Article
Oncology
Dongjoon Ko, Semi Kim
Article
Medicine, Research & Experimental
Hye-Mi Ahn, Jihye Ryu, Jin Myeong Song, Yunhee Lee, Hye-Jin Kim, Dongjoon Ko, Inpyo Choi, Sang Jick Kim, Jung Weon Lee, Semi Kim
Article
Biochemistry & Molecular Biology
Amir Abbas Mokhtarieh, Jieun Lee, Semi Kim, Myung Kyu Lee
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2018)
Article
Cell Biology
Jae Woo Jung, Stephani Joy Y. Macalino, Minghua Cui, Ji Eon Kim, Hye-Jin Kim, Dae-Geun Song, Seo Hee Nam, Semi Kim, Sun Choi, Jung Weon Lee
Article
Multidisciplinary Sciences
Solbi Kim, Dongjoon Ko, Yunhee Lee, Seonghui Jang, Younghoon Lee, Ill Young Lee, Semi Kim
SCIENTIFIC REPORTS
(2019)
Article
Oncology
Jihye Ryu, Eunmi Kim, Min-Kyung Kang, Dae-Geun Song, Eun-Ae Shin, Haesong Lee, Jae Woo Jung, Seo Hee Nam, Ji Eon Kim, Hye-Jin Kim, Taekwon Son, Semi Kim, Hwi Young Kim, Jung Weon Lee
Summary: The study revealed the crucial role of TM4SF5 in the progression of nonalcoholic steatohepatitis (NASH), including inducing lipid accumulation, inflammatory responses, and fibrosis in liver tissue. TM4SF5, along with signaling pathways involving SIRT1, SREBPs, and SOCS/STAT3, promoted the development of NASH.
JOURNAL OF PATHOLOGY
(2021)
Article
Pharmacology & Pharmacy
Atanu Naskar, Sohee Lee, Yunhee Lee, Semi Kim, Kwang-sun Kim
Article
Oncology
Yunhee Lee, Dongjoon Ko, Junghwa Yoon, Younghoon Lee, Semi Kim
Summary: TMEM52B has been identified as a novel gene expressed widely in normal human tissues. Suppression of TMEM52B in colon cancer cells leads to enhanced epithelial-mesenchymal transition (EMT), invasion, and survival, potentially through multiple signaling pathways. Clinical data suggest that high TMEM52B expression is associated with increased patient survival in various types of cancer.
JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH
(2021)
Article
Pharmacology & Pharmacy
Hyejin Cho, Atanu Naskar, Sohee Lee, Semi Kim, Kwang-Sun Kim
Summary: A nickel-doped zinc oxide and black phosphorus nanocomposite displayed a strong synergistic effect with polymyxin B against polymyxin-resistant E. coli, neutralizing the charge on the bacterial surface and facilitating the incorporation of polymyxin B. The combination was shown to be biocompatible and has the potential to revive polymyxins as effective antibiotics against resistant Gram-negative bacterial infections.
Article
Oncology
Yunhee Lee, Junghwa Yoon, Dongjoon Ko, Minyeong Yu, Soojin Lee, Semi Kim
Summary: TMPRSS4 promotes cancer stem cell features in prostate cancer by upregulating the stem cell factor SOX2 through the EMT-inducing transcription factors SLUG and TWIST1. This novel mechanism may play a crucial role in tumor progression control.
JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH
(2021)