Review
Nutrition & Dietetics
Valentin Lacombe, Guy Lenaers, Geoffrey Urbanski
Summary: Cobalamin and transcobalamins play important roles in cancer development, affecting cellular metabolism and being associated with cancer diagnosis and prognosis.
Article
Biology
Alan D. Winter, Elissa Tjahjono, Leonardo J. Beltran, Iain L. Johnstone, Neil J. Bulleid, Antony P. Page
Summary: This study describes a new SAM methyltransferase, RIPS-1, identified through forward genetic screens in Caenorhabditis elegans for resistance to the thiol-reducing agent DTT. The research shows that dietary vitamin B12 plays a central role in normal metabolic processes and counters reductive stress in C. elegans. RIPS-1 is identified as a novel methyltransferase in the methionine cycle.
Review
Biochemistry & Molecular Biology
Mackenzie Newman, Heather Connery, Jonathan Boyd
Summary: Opioids are widely used but have side effects such as central respiratory depression. Vitamin C has been shown to reduce these effects, but the underlying mechanisms are unclear. This review provides a signaling framework for the interaction between MOR activation and vitamin C, indicating the need for further research on their crosstalk in alleviating the negative side effects of opioids.
Article
Environmental Sciences
Jie Xu, Xin-Ling Wang, Han-Fang Zeng, Zhao-Yu Han
Summary: In this study, the regulatory effect and mechanism of methionine (Met) in alleviating heat stress-induced ferroptosis in bovine mammary epithelial cells were investigated. The results showed that Met increased the expression levels of GPX4, SLC7A11, and FTH1 by activating Nrf2, resulting in a decrease in LIP and lipid ROS levels, thus alleviating heat stress-induced ferroptosis. These findings highlight the significant role of Met in mitigating bovine mammary gland injury caused by heat stress.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2023)
Article
Agriculture, Dairy & Animal Science
J. Lu, J. T. Weil, P. Maharjan, M. K. Manangi, S. Cerrate, C. N. Coon
Summary: The study aimed to investigate the kinetics of methionine metabolism in 18-day-old chick embryos by administering three isotopic tracers and analyzing intermediate metabolites. Results showed that supplementation of folic acid and pyridoxine in breeder diets affected the hepatic concentration of homocysteine and spermine in embryos. Furthermore, it was found that deficient levels of pyridoxine in breeder diets led to an increase in concentration of intermediate metabolites of methionine and polyamines in embryos.
Article
Biochemistry & Molecular Biology
Stacey L. Borrego, Johannes Fahrmann, Jue Hou, Da-Wei Lin, Bruce J. Tromberg, Oliver Fiehn, Peter Kaiser
Summary: The study reveals that Met stress in triple-negative breast cancer cells leads to a global decrease in lipid abundances, except for triglycerides, and an increase in lipid droplets. Additionally, specific gene expression changes were observed in response to Met stress in the cells.
JOURNAL OF LIPID RESEARCH
(2021)
Article
Cell Biology
Ziad Hassan, David Coelho, Carine Bossenmeyer-Pourie, Karim Matmat, Carole Arnold, Aurelie Savladori, Jean-Marc Alberto, Remy Umoret, Jean-Louis Gueant, Gregory Pourie
Summary: Impairment of one-carbon metabolism during pregnancy, either due to nutritional deficiencies in B9 or B12 vitamins or caused by specific genetic defects, is often associated with neurological defects, including cognitive dysfunction that persists even after vitamin supplementation. Using a knock-out model for the methionine synthase specifically in the brain, researchers found that the absence of methionine synthase did not lead to growth retardation in offspring. However, there were temporary retardations in vegetative functions during the suckling period and a significant reduction in cognitive performance after weaning. Investigation also showed a reduction of AMPA receptors phosphorylation and clustering in cognitive areas, indicating an epigenomic effect of the neuronal deficiency of methionine synthase on the reduction of glutamatergic synapses excitability.
Article
Multidisciplinary Sciences
Rong Li, Chao Guo, Xiao Lin, Ting Fung Chan, Min Su, Zhiyong Zhang, Keng Po Lai
Summary: Vitamin C supplementation reduces PFOA-induced liver damage by controlling signaling networks, suppressing linoleic acid metabolism, reducing thiodiglycolic acid, and elevating glutathione levels in the liver.
JOURNAL OF ADVANCED RESEARCH
(2022)
Article
Cell Biology
Erinn Zixuan Sim, Takayuki Enomoto, Nobuaki Shiraki, Nao Furuta, Soshiro Kashio, Taiho Kambe, Tomonori Tsuyama, Akihiro Arakawa, Hiroki Ozawa, Mizuho Yokoyama, Masayuki Miura, Shoen Kume
Summary: The study found that methionine deprivation triggers a reduction in intracellular Zn content and upregulation of Zn exporter, leading to differentiation. PSCs cultured under Zn deprivation exhibit slowed cell growth and altered methionine metabolism-related metabolite profile.
Article
Biology
G. Gokul, Jogender Singh
Summary: This study reveals that the redox reagent DTT can induce stress in the endoplasmic reticulum and disrupt protein folding, leading to cellular toxicity. The presence of vitamin B12 in the bacterial diet can alleviate DTT toxicity. Loss of function of the SAM-dependent methyltransferase gene R08E5.3 confers resistance to DTT. Depletion of SAM is identified as the underlying mechanism of DTT toxicity. The unfolded protein response pathway involving IRE-1/XBP-1 is required to counteract DTT toxicity at high concentrations.
Article
Horticulture
Magdalena Valsikova-Frey, Jiri Mlcek, Martina Buckova, Anna Adamkova, Martin Adamek, Tunde Jurikova
Summary: This study investigated the influence of cultivation method on green pepper fruits. The results showed that the mulching film had a positive effect on pepper yield, while the variant without mulching film performed better in terms of vitamin C and soluble solids content.
Article
Multidisciplinary Sciences
Hongyan Liu, Fang Zhou, Ting Zhou, Yuanxiao Yang, Yingzhong Zhao
Summary: Sesame leaves, especially the wrinkled mutant JQA, are a rich source of nutrients, including high-quality oil, crude protein, amino acids, crude fiber, cellulose or hemicellulose, sugars, vitamins, and flavones. Compared to other common green leafy vegetables, the JQA mutant has higher levels of unsaturated acid, calcium, potassium, as well as vitamins B-6, B-2, C, and D. The wrinkled leaves can be consumed as fresh vegetables or preserved in a dry state, providing high recommended daily intakes (RDIs) of nutrients.
SCIENTIFIC REPORTS
(2022)
Review
Chemistry, Multidisciplinary
Bogdan Marciniec, Cezary Pietraszuk, Piotr Pawluc, Hieronim Maciejewski
Summary: This Review summarizes the developments in inorganometallic catalysis over the last 15 years, highlighting the importance of inorganometallics in the synthesis of metalloid compounds and advanced organic synthesis.
Article
Fisheries
Qincheng Huang, Xiaodan Wang, Zhideng Lin, Jiadai Liu, Han Wang, Cong Zhang, Zhenyu Du, Jianguang Qin, Liqiao Chen
Summary: The present study investigated the effect of vitamin A on growth performance, lipid metabolism, fatty acid composition, antioxidant ability and inflammatory response of crabs fed different vegetable oils. The interaction between vitamin A and lipid source did not influence the survival rate, final body weight, weight gain and specific growth rate of crabs. Crabs fed perilla oil showed optimal growth performance with the lowest body lipid content and hemolymph triglyceride, while crabs fed coconut oil showed the worst growth performance and aggravating inflammation. Dietary vitamin A improved growth performance and relieved inflammation of crabs fed different lipid sources, enhancing antioxidant activity and mitigating lipid peroxidation.
Article
Oncology
Karim Matmat, Jean-Baptiste Conart, Paul-Henri Graindorge, Sandra El Kouche, Ziad Hassan, Youssef Siblini, Remy Umoret, Ramia Safar, Okan Baspinar, Aurelie Robert, Jean-Marc Alberto, Abderrahim Oussalah, David Coelho, Jean-Louis Gueant, Rosa-Maria Gueant-Rodriguez
Summary: This study used an original mouse model to investigate cblG inherited retinopathy and found that altered DNA methylation plays a pivotal role in eye development, cone differentiation, and retinoid metabolism.
CLINICAL EPIGENETICS
(2023)
Article
Microbiology
Suni Anie Mathew, Marjo Helander, Kari Saikkonen, Radomira Vankova, Petre I. I. Dobrev, Serdar Dirihan, Benjamin Fuchs
Summary: This study found that the endophytic microbial community in meadow fescue differs between leaf and root tissues, and the fungal community in the leaves is different between Epichloe-symbiotic and Epichloe-free plants. Epichloe symbiosis alters phytohormone concentrations and enhances plant performance in biomass and seed mass. The results suggest that Epichloe endophytes affect plant immune responses and other fungal endophytes through changes in leaf fungal microbiota and phytohormone concentrations.
Article
Plant Sciences
Rakesh K. Upadhyay, Vaclav Motyka, Eva Pokorna, Petre I. Dobrev, Jozef Lacek, Jonathan Shao, Kim S. Lewers, Autar K. Mattoo
Summary: High performance liquid chromatography electrospray tandem-mass spectrometry (HPLC-ESI-MS/MS) was used to study the regulation of endogenous phytohormones in octoploid strawberries during fruit ripening. ABA, ACC, and iP were found to be associated with ripening, while ABA catabolites mimicked the pattern of climacteric decline. ACC accumulation and the presence of a large ACS gene family in octoploid strawberries suggest their involvement in fruit ripening.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2023)
Article
Agronomy
Milan Novak, Veronika Zemanova, Milan Pavlik, Simona Prochazkova, Daniela Pavlikova
Summary: In a pot experiment, the influence of toxic elements on enzymatic activity, dissolved organic carbon, element accumulation, and root biomass in two fern species was evaluated. The results showed an increasing trend in element contents in the roots for both ferns with soil contamination. Significant differences were observed in arsenic and zinc accumulation between the two fern species. Pteris cretica accumulated significantly higher arsenic, while P. straminea showed an opposite trend in zinc accumulation. These findings suggest higher sensitivity of P. straminea to toxic element contamination, leading to increased enzymatic activity and dissolved organic carbon content in the root zone.
PLANT SOIL AND ENVIRONMENT
(2023)
Article
Biochemistry & Molecular Biology
Ilya E. Zlobin, Radomira Vankova, Petre I. Dobrev, Alena Gaudinova, Alexander V. Kartashov, Yury V. Ivanov, Alexandra I. Ivanova, Vladimir V. Kuznetsov
Summary: Delayed or incomplete recovery of gas exchange after water stress relief limits assimilation in the post-drought period. ABA accumulation and antagonistic action between ABA and CKs play important roles in regulation of stomatal conductance under water deficit. However, in greenhouse-grown 3-year-old Scots pine saplings recovering from water stress, sustained ABA accumulation and decreased CK/ABA ratio were not the main causes of delayed recovery of stomatal conductance.
Review
Horticulture
Tatjana Cosic, Martin Raspor, Vaclav Motyka, Aleksandar Cingel, Slavica Ninkovic
Summary: This article summarizes the results of a decade-long study on in vitro morphogenesis of kohlrabi. Efficient regeneration through somatic embryogenesis and de novo shoot organogenesis has been achieved using minimal external application of plant growth regulators. The article also discusses the role of phytohormones and nutrient media in regulating the organogenic response. These studies provide valuable insights into in vitro regeneration of kohlrabi and phytohormonal regulation, and pave the way for the development of more resistant varieties.
Article
Environmental Sciences
Anna Mascellani, Filip Mercl, Sebnem Kurhan, Lorenzo Pierdona, Jiri Kudrna, Veronika Zemanova, Frantisek Hnilicka, Pavel Kloucek, Pavel Tlustos, Jaroslav Havlik
Summary: In this study, the researchers investigated the effects of carbamazepine contamination on Zea mays L. cv. Ronaldinio at different phenological stages. They found that carbamazepine was transferred to the aboveground and root biomass in a dose-dependent manner. The contamination mainly affected the 4th leaf phenological stage, with decreased photosynthetic rate, reduced activity of photo-system II, decreased water potential, decreased carbohydrates and gamma-aminobutyric acid in roots, and increased phenylpropanoids in aboveground biomass. Older plants showed adaptation and minor effects in the presence of the contaminant.
Article
Plant Sciences
Sofija Stupar, Vaclav Motyka, Petre I. Dobrev, Tatjana Cosic, Nina Devrnja, Ljiljana Tubic, Jelena Savic
Summary: The study investigated the comprehensive phytohormone profile of potato plants exposed to French marigold essential oil (EO) for different time periods. The results showed altered phytohormone responses of EO-exposed potato plants, with stress-related phytohormones being the most affected. The expression levels of genes involved in biosynthesis and catabolism of stress-related phytohormones indicated that the jasmonic acid biosynthetic pathway was the most affected.
JOURNAL OF PLANT GROWTH REGULATION
(2023)
Article
Biochemistry & Molecular Biology
Anna Zaharieva, Krasimir Rusanov, Mila Rusanova, Momchil Paunov, Zhenya Yordanova, Desislava Mantovska, Ivanka Tsacheva, Detelina Petrova, Kiril Mishev, Petre I. Dobrev, Jozef Lacek, Roberta Filepova, Grigor Zehirov, Valya Vassileva, Danijela Misic, Vaclav Motyka, Ganka Chaneva, Miroslava Zhiponova
Summary: This study compared the chemical profiles of in vitro shoots and wild-grown N. nuda plants through metabolomic analysis. The results showed differences in volatile compositions between different parts of the plants, with characteristic iridoids found in both in vitro shoots and flowers of wild-grown plants, and abundant monoterpenes in the leaves of wild-grown plants. Furthermore, compounds associated with known biological activities exhibited consistent associations with the identified metabolic profiles in different fractions.
Article
Plant Sciences
Michael Derevyanchuk, Serhii Kretynin, Yaroslava Bukhonska, Igor Pokotylo, Vladimir Khripach, Eric Ruelland, Roberta Filepova, Petre I. Dobrev, Jan Martinec, Volodymyr Kravets
Summary: This study analyzed the impact of 24-epicastasterone on hormone content in soybean leaves and found that it upregulated auxin levels while downregulating salicylic acid, jasmonic acid, and abscisic acid levels. It also activated cell metabolism's adaptation strategies to environmental challenges.
Article
Environmental Sciences
Daniela Pavlikova, Veronika Zemanova, Milan Pavlik
Summary: In a pot experiment, three garden vegetables-radish, carrot, and lettuce-were cultivated using soils polluted mainly by cadmium, zinc, lead, and chromium from the P.ribram area in Czech Republic. The results showed that lettuce absorbed the highest amounts of toxic elements, followed by radish. The macronutrient contents in the edible parts did not change significantly, but there was an accumulation of free amino acids in all vegetables, with the highest content being in radish. The risk to human health was evaluated using target hazard quotient and total hazard index, which indicated a possible non-carcinogenic health effect from consuming lettuce and carrot.