Article
Pharmacology & Pharmacy
Taiyue Li, Xiaoyi Yu, Xuerui Zhu, Yuanyuan Wen, Meizhen Zhu, Weiwei Cai, Bao Hou, Fei Xu, Liying Qiu
Summary: This study found that vaccarin can alleviate endothelial inflammatory injury in diabetes by mediating the miR-570-3p/HDAC1 pathway. The research provides new insights into the molecular mechanisms of diabetic endothelial dysfunction and vascular complications.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Oncology
Sofia Elena Tesolato, Daniel Gonzalez-Gamo, Ana Barabash, Paula Claver, Sofia Cristina de la Serna, Inmaculada Dominguez-Serrano, Jana Dziakova, Carmen de Juan, Antonio Jose Torres, Pilar Iniesta
Summary: This study aims to analyze and correlate the expression levels of four miRNAs associated with cancer and obesity in colorectal cancer (CRC) patients and a control group. The results indicate the significance of hsa-miR-143-3p and hsa-miR-181a-5p in the clinical management of CRC.
Article
Cell Biology
Yan Tang, Li-Jie Yang, Hao Liu, Yan-Jue Song, Qi-Qi Yang, Yang Liu, Shu-Wen Qian, Qi-Qun Tang
Summary: Obesity, especially increased visceral fat, is associated with metabolic challenges such as atherosclerosis, but the exact mechanism is not fully understood. This study aims to investigate the role of visceral-fat-derived exosomes in endothelial cells and atherosclerosis. The research shows that obesity alters the miRNA profile of adipose exosomes, and exosomal miR-27b-3p can enter endothelial cells and activate the NF-kB pathway by suppressing PPARa. In ApoE-deficient mice, administration of miR-27b-3p mimic increases inflammation and atherogenesis, while overexpression of PPARa protects against atherosclerosis. Therefore, obesity-induced exosomal miR-27b-3p promotes endothelial inflammation and facilitates atherogenesis through PPARa suppression.
Article
Biochemistry & Molecular Biology
Marta Gil-Martinez, Clara Lorente-Sorolla, Jose M. Rodrigo-Munoz, Sara Naharro, Zahara Garcia-de Castro, Joaquin Sastre, Marcela Valverde-Monge, Santiago Quirce, Maria L. Caballero, Jose M. Olaguibel, Victoria del Pozo
Summary: Clarifying inflammatory processes and categorising asthma into phenotypes and endotypes improves asthma management. Obesity worsens severe asthma and reduces quality of life, although its specific molecular impact remains unclear. We aimed to study hsa-miR-26a-1-3p, hsa-miR-376a-3p, and their target genes in asthmatic subjects with or without obesity to find biomarkers and comprehend obese asthma mechanisms.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biotechnology & Applied Microbiology
Zifu Zhu, Li Ma
Summary: Postoperative cognitive dysfunction (POCD) is a normal condition that develops after surgery with anesthesia, and the mechanisms behind it are still unknown. This study reveals that sevoflurane induces neuroinflammation by increasing the expression of long non-coding RNA Gm5106, which is regulated by transcription factor Hoxa5 and miR-27b-3p. This forms a positive feedback loop in the context of sevoflurane stimulation.
Article
Biochemistry & Molecular Biology
Issei Seki, Hiroto Izumi, Naomichi Okamoto, Atsuko Ikenouchi, Yasuo Morimoto, Seichi Horie, Reiji Yoshimura
Summary: This study examined the association between miRNA levels in extracellular vesicles (EVs) from serum and the severity of Major Depression (MD). The expression levels of hsa-miR-6813-3p and hsa-miR-2277-3p were found to be significantly downregulated with increasing depression severity of MD. These miRNAs could serve as novel biomarkers for MD and contribute to understanding its pathophysiology.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell Biology
Tian Xia, Zhiyong Zhang, Xin Zhang, Quanfu Li
Summary: This study aimed to determine the effect of the interaction between miR-186-3p and KRT18 on the biological behavior of colon cancer cells. The results showed that miR-186-3p suppresses proliferation and viability, but facilitates apoptosis in colon cancer cells by targeting KRT18 and negatively regulating the MAPK signaling pathway.
Article
Pediatrics
Salvatore Tagliaferri, Pasquale Cepparulo, Antonio Vinciguerra, Marta Campanile, Giuseppina Esposito, Giuseppe Maria Maruotti, Fulvio Zullo, Lucio Annunziato, Giuseppe Pignataro
Summary: The current tests for diagnosing fetal hypoxia in-utero lack sensitivity, but emerging evidence suggests that microRNAs from the placenta circulating in maternal blood during pregnancy could serve as non-invasive biomarkers for pregnancy complications. By examining the expression of certain microRNAs regulated by hypoxia in maternal blood samples, researchers were able to identify potential markers for fetal growth restriction (FGR) and new therapeutic targets. Measurement of these microRNAs in maternal blood may lead to a future diagnostic test to determine the degree of fetal hypoxia in FGR, paving the way for appropriate therapeutic interventions.
FRONTIERS IN PEDIATRICS
(2021)
Article
Biochemistry & Molecular Biology
Yu-Jen Chen, Chia-Tien Hsu, Shang-Feng Tsai, Cheng-Hsu Chen
Summary: Chronic allograft dysfunction (CAD) is a major condition affecting long-term kidney graft survival. In this study, the correlation between plasma miR-21 and the severity of CAD was investigated. RNA sequencing was performed and differentially expressed miRNAs in CAD were identified. MiR-21-5p, miR-20a-5p, and miR-101-3p were found to be associated with the TGF-beta pathway and CAD. These findings may lead to the development of early prediction miRNA biomarkers and therapeutic tools for CAD in the field of kidney transplantation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Yuan Li, Qingya Dang, Zhiyi Li, Chuting Han, Yan Yang, Miaoling Li, Pengyun Li
Summary: This study found that acacetin protects against endothelial dysfunction in hypertension by activating the AKT/eNOS pathway and modulating mitochondrial function. The findings suggest that acacetin may be a potential therapeutic agent for the prevention and treatment of hypertension.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Genetics & Heredity
Ricardo I. Peraza-Vega, Mahara Valverde, Emilio Rojas
Summary: miR-27b-3p acts as a crucial negative regulator of DNA repair when overexpressed, reducing the cell's ability to repair DNA damage.
Article
Cardiac & Cardiovascular Systems
Guoqi Li, Yihui Shao, Hong-Chang Guo, Ying Zhi, Bokang Qiao, Ke Ma, Jie Du, Yong-Qiang Lai, Yulin Li
Summary: miR-27b-3p induces pathological cardiac remodelling and inhibition of endogenous miR-27b-3p or administration of FGF1 might suppress cardiac remodelling.
CARDIOVASCULAR RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Xin Yang, Zhenhui Li, Zhijun Wang, Jiaao Yu, Manting Ma, Qinghua Nie
Summary: In this study, the researchers found differential expression of miR-27b-3p and Cbl-b genes in an induced atrophy model. They confirmed that miR-27b-3p can regulate the expression of Cbl-b gene. Cbl-b gene plays an important role in muscle atrophy, as its overexpression can upregulate the expression of marker genes related to muscle atrophy, while silencing Cbl-b expression can alleviate muscle atrophy.
Article
Chemistry, Multidisciplinary
Yufei Liu, Zhiling Zhang, Biao Wang, Yunsheng Dong, Congrui Zhao, Yanhong Zhao, Lin Zhang, Xiangsheng Liu, Jingyue Guo, Yuehua Chen, Jie Zhou, Tingting Yang, Yanying Wang, Hao Liu, Shufang Wang
Summary: Small extracellular vesicles (sEVs) secreted by mesenchymal stem cells (MSCs) have been extensively studied. When MSCs are exposed to an inflammatory environment, they release more functional factors to regulate the inflammatory microenvironment and promote tissue regeneration. Inflammatory-stimulated sEVs show enhanced functions in cell proliferation, migration, and macrophage polarization. In vivo experiments demonstrate that modified sEVs have significant therapeutic effects on temporomandibular joint regeneration.
Article
Agriculture, Multidisciplinary
Jiaxin Li, Hengju Ge, Yang Xu, Jiahong Xie, Fujie Yan, Wei Chen
Summary: Geniposide (GEN) can eliminate excess intracellular free radicals by activating the Nrf2/ARE signaling pathway in hepatic cells, thereby inhibiting oxidative stress and enhancing antioxidant capacity. Additionally, GEN can suppress the upregulation of miR-27b-3p induced by H2O2, leading to improved repair of oxidative damage.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Cardiac & Cardiovascular Systems
Alessandro Spirito, Lukas Vaisnora, Athanasios Papadis, Fortunato Iacovelli, Celestino Sardu, Alexandra Selberg, Sarah Baer, Raminta Kavaliauskaite, Fabrice Temperli, Babken Asatryan, Thomas Pilgrim, Lukas Hunziker, Dik Heg, Marco Valgimigli, Stephan Windecker, Lorenz Raeber
Summary: This study assessed the frequency, predictors, and clinical impact of acute coronary occlusion in hemodynamically stable and unstable out-of-hospital cardiac arrest patients without ST-segment elevation. The results showed that acute coronary occlusions were found in both stable and unstable patients and were associated with a higher risk of cardiovascular death.
JOURNAL OF THE AMERICAN COLLEGE OF CARDIOLOGY
(2023)
Article
Medicine, General & Internal
Raffaele Marfella, Celestino Sardu, Nunzia D'Onofrio, Carlo Fumagalli, Lucia Scisciola, Ferdinando Carlo Sasso, Mario Siniscalchi, Ludovica Vittoria Marfella, Davide D'Andrea, Fabio Minicucci, Giuseppe Signoriello, Arturo Cesaro, Maria Consiglia Trotta, Chiara Frige, Francesco Prattichizzo, Maria Luisa Balestrieri, Antonio Ceriello, Paolo Calabro, Ciro Mauro, Luca del Viscovo, Giuseppe Paolisso
Summary: This study evaluated the incidence of intra-stent restenosis (ISR)-related events in patients with type 2 diabetes (T2DM) and acute myocardial infarction (AMI) treated or not with sodium/glucose cotransporter 2 inhibitors (SGLT2i). The results showed that the use of SGLT2 inhibitors in patients with T2DM is associated with a reduced incidence of ISR-related events, independent of glycemic control.
Article
Biochemistry & Molecular Biology
Angelica Giuliani, Jacopo Sabbatinelli, Stefano Amatori, Laura Graciotti, Andrea Silvestrini, Giulia Matacchione, Deborah Ramini, Emanuela Mensa, Francesco Prattichizzo, Lucia Babini, Domenico Mattiucci, Elena Marinelli Busilacchi, Maria Giulia Bacalini, Emma Espinosa, Fabrizia Lattanzio, Antonio Domenico Procopio, Fabiola Olivieri, Antonella Poloni, Mirco Fanelli, Maria Rita Rippo
Summary: This study found that the expression of MeCP2 protein is downregulated during adipogenic differentiation of hBMSCs. This downregulation is not dependent on MeCP2 DNA methylation or mRNA levels, but on miRNA regulation. miR-422a and miR-483-5p are upregulated during adipogenic differentiation of hBMSCs. Modulation of miR-422a and miR-483-5p levels can affect MeCP2 expression and adipogenic differentiation.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2023)
Article
Endocrinology & Metabolism
Francesco Prattichizzo, Chiara Frige, Rosalba La Grotta, Antonio Ceriello
Summary: Body weight variability is independently associated with cardiovascular diseases and mortality, and it may interact with other risk factors to increase cardiovascular risk. In addition, weight variability is also linked to microvascular complications in people with type 2 diabetes.
DIABETES RESEARCH AND CLINICAL PRACTICE
(2023)
Article
Agriculture, Dairy & Animal Science
Gianluca Neglia, Serena Calabro, Alessio Cotticelli, Angela Salzano, Roberta Matera, Alessandro Vastolo, Nunzia D'Onofrio, Andrea Giorgino, Elisa Martino, Maria L. Balestrieri, Giuseppe Campanile
Summary: The inclusion of former food products in the diet of buffaloes as an alternative to green forage did not significantly affect fermentation characteristics, milk yield and quality, and the content of certain biomolecules and total antioxidant activity. Therefore, biscuit meal can be used as a substitute for green fodder to ensure environmental sustainability and optimize costs without compromising milk quality.
JOURNAL OF ANIMAL PHYSIOLOGY AND ANIMAL NUTRITION
(2023)
Article
Cardiac & Cardiovascular Systems
Raffaele Marfella, Francesco Prattichizzo, Celestino Sardu, Pasquale Paolisso, Nunzia D'Onofrio, Lucia Scisciola, Rosalba La Grotta, Chiara Frige, Franca Ferraraccio, Iacopo Panarese, Mara Fanelli, Piero Modugno, Antonio Maria Calafiore, Mario Melchionna, Ferdinando Carlo Sasso, Fulvio Furbattoj, Davide D'Andreaj, Mario Siniscalchi, Ciro Mauro, Arturo Cesaro, Paolo Calabro, Gaetano Santulli, Maria Luisa Balestrieri, Emanuele Barbato, Antonio Ceriello, Giuseppe Paolisso
Summary: This study found that PCSK9 inhibitors, used as monotherapy, can reduce the expression of inflammatory markers within atherosclerotic plaques and increase the abundance of SIRT3 and collagen. Patients treated with PCSK9 inhibitors had a decreased risk of cardiovascular events. Additionally, the expression of PCSK9 was positively correlated with the expression of pro-inflammatory proteins within the plaque, and the inflammatory burden was associated with an increased risk of cardiovascular events.
Article
Endocrinology & Metabolism
Pasquale Paolisso, Luca Bergamaschi, Arturo Cesaro, Emanuele Gallinoro, Felice Gragnano, Celestino Sardu, Niya Mileva, Alberto Foa, Matteo Armillotta, Angelo Sansonetti, Sara Amicone, Andrea Impellizzeri, Marta Belmonte, Giuseppe Esposito, Nuccia Morici, Jacopo Andrea Oreglia, Gianni Casella, Ciro Mauro, Dobrin Vassilev, Nazzareno Galie, Gaetano Santulli, Paolo Calabro, Emanuele Barbato, Raffaele Marfella, Carmine Pizzi
Summary: This study analyzed the association between chronic SGLT2-I treatment and the development of CI-AKI in diabetic patients with AMI undergoing PCI. The results showed that SGLT2-I users had a lower incidence of CI-AKI, especially in patients without CKD. Therefore, the use of SGLT2-I may reduce the risk of CI-AKI in patients with AMI.
DIABETES RESEARCH AND CLINICAL PRACTICE
(2023)
Article
Medicine, General & Internal
Celestino Sardu, Gianluca Gatta, Gorizio Pieretti, Nunzia D' Onofrio, Maria Luisa Balestrieri, Lucia Scisciola, Salvatore Cappabianca, Giuseppe Ferraro, Giovanni Francesco Nicoletti, Giuseppe Signoriello, Liberata Sportiello, Gianluigi Savarese, Mario Melchionna, Feliciano Ciccarelli, Daniele La Forgia, Giuseppe Paolisso, Raffaele Marfella
Summary: This study evaluated the expression of SGLT2, inflammatory cytokines, and sirtuins in breast fat tissue and serum cytokines of pre-menopausal women. The results showed that women with fatty breasts over-expressed SGLT2 and inflammatory cytokines, while downregulating breast sirtuins. The study also found correlations between the expression of SGLT2/cytokines/sirtuins and clinical variables related to cardiovascular health.
EUROPEAN JOURNAL OF INTERNAL MEDICINE
(2023)
Editorial Material
Medicine, General & Internal
Celestino Sardu, Raffele Marfella
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Medicine, General & Internal
Domenico Cozzolino, Ciro Romano, Celestino Sardu, Riccardo Nevola, Giuseppina Rosaria Umano, Luca Rinaldi, Luigi Elio Adinolfi, Christian Catalini, Aldo Marrone, Maurizio Municino, Ferdinando Carlo Sasso, Raffaele Marfella
Summary: Limited data are available on long-term outcomes among COVID-19 patients beyond the acute phase. This study evaluated the one-year all-cause mortality and factors/conditions associated with death among patients discharged from a COVID center. The study found that older age, cancer, heart failure, arrhythmias, elevated biomarker levels, and impaired renal function were independently associated with death. The overall mortality rate at one-year follow-up was 9%.
JOURNAL OF CLINICAL MEDICINE
(2023)
Review
Endocrinology & Metabolism
Giovanni Antonio Silverii, Matteo Monami, Marco Gallo, Alberto Ragni, Francesco Prattichizzo, Valerio Renzelli, Antonio Ceriello, Edoardo Mannucci
Summary: Our meta-analysis suggests that GLP-1RA treatment may be associated with a moderate increase in relative risk for thyroid cancer in clinical trials, with a small increase in absolute risk. Longer duration studies are needed to assess the clinical implications of this finding.
DIABETES OBESITY & METABOLISM
(2023)
Review
Medicine, Research & Experimental
Puja Ghosh, Rosaria Anna Fontanella, Lucia Scisciola, Ada Pesapane, Fatemeh Taktaz, Martina Franzese, Armando Puocci, Antonio Ceriello, Francesco Prattichizzo, Maria Rosaria Rizzo, Giuseppe Paolisso, Michelangela Barbieri
Summary: Reactive oxygen species (ROS) play important roles in regulating various cellular processes and can impact cell survival, death, inflammation, differentiation, growth, and immune response. Pathological conditions like diabetes can increase ROS production, leading to protein and DNA damage, neurodegeneration, and cognitive decline. GLP-1RAs, a type of anti-diabetic drug, have been found to reduce ROS generation and improve cognitive function.
Article
Endocrinology & Metabolism
Francesco Prattichizzo, Antonio Ceriello
Summary: This article reviews the importance of glycated hemoglobin (HbA1c) in diabetes management and highlights its limitations in capturing blood glucose fluctuations. Recent data suggest that variability in blood glucose and HbA1c are additional independent risk factors for diabetes complications. Continuous glucose monitoring allows calculation of time in range (TIR), a new metric related to glucose variability and an independent risk factor for complications.