Article Exosomal miR-27b-3p secreted by visceral adipocytes contributes to endothelial inflammation and atherogenesis

Obesity, particularly increased visceral fat, positively correlates with various metabolic challenges, including atherosclerosis, but the mechanism is not fully understood. The aim of this study is to determine the role of visceral-fat-derived exosomes (Exo) in endothelial cells and atherosclerosis. We show that obesity changes the miRNA profile of visceral adipose exosomes in mice. Importantly, exosomal miR-27b-3p effi-ciently enters into the vascular endothelial cells and activates the NF-kB pathway by downregulating PPARa. Mechanistically, miR-27b-3p binds directly to the CDS region of PPARa mRNA, thereby promoting mRNA degradation and suppressing translation. In ApoE-deficient mice, administration of miR-27b-3p mimic increases inflammation and atherogenesis, while overexpression of PPARa protects against atherosclerosis. Thus, obesity-induced exosomal miR-27b-3p promotes endothelial inflammation and facilitates atherogen-esis by PPARa suppression. We reveal an exosomal pathway by which obesity aggravates atherosclerosis and proposed therapeutic strategies for atherosclerosis in people with obesity.

Automated summary

Obesity, especially increased visceral fat, is associated with metabolic challenges such as atherosclerosis, but the exact mechanism is not fully understood. This study aims to investigate the role of visceral-fat-derived exosomes in endothelial cells and atherosclerosis. The research shows that obesity alters the miRNA profile of adipose exosomes, and exosomal miR-27b-3p can enter endothelial cells and activate the NF-kB pathway by suppressing PPARa. In ApoE-deficient mice, administration of miR-27b-3p mimic increases inflammation and atherogenesis, while overexpression of PPARa protects against atherosclerosis. Therefore, obesity-induced exosomal miR-27b-3p promotes endothelial inflammation and facilitates atherogenesis through PPARa suppression.