Review
Pharmacology & Pharmacy
Pawel Krzyzek, Beatrice Marinacci, Irene Vitale, Rossella Grande
Summary: The proper functioning of the human body has been extensively studied, and probiotics have been found to have a significant impact on human health through their production of extracellular vesicles. These vesicles contribute to maintaining the balance of microorganisms, regulating the immune system, enhancing digestion, and preventing or alleviating diseases. However, the health-promoting effects of extracellular vesicles secreted by non-pathogenic microorganisms, including probiotics, are not well understood. This review article aims to provide the latest literature data on the health-inducing properties of probiotics' extracellular vesicles, including their physicochemical properties and proteome.
Review
Microbiology
A. Paula Dominguez Rubio, Cecilia L. D'Antoni, Mariana Piuri, Oscar E. Perez
Summary: Probiotics have demonstrated potential health benefits against infectious diseases, with extracellular vesicles possibly playing an important role. Further research on probiotic-derived EVs could lead to new therapeutic avenues.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Food Science & Technology
Xiaolin Liu, Xuepeng Lv, Yue Sun, Chunhong Liu, Rongchun Wang, Rongmei Liu, Ying Ma, Qiming Li
Summary: The probiotic characteristics of Lacticaseibacillus rhamnosus grx10 were investigated through genotype and phenotype analysis in this study. In vitro experiments showed that grx10 exhibited excellent resistance to ambient stresses, bacteriostatic activity, and adhesion ability, indicating its potential as a probiotic. Complete genome sequencing revealed the genetic information and genes associated with probiotic characteristics, as well as the prediction of genes involved in extracellular proteins and secretory pathways. Among the secreted proteins, p40 and p75 were found to promote intestinal barrier integrity, with an abundance of 0.36% and 0.20% in the fermented supernatant of grx10. This study highlights the potential of grx10 in protecting the intestinal barrier from a postbiotic perspective.
Review
Biochemistry & Molecular Biology
Rui Miguel Marques Bernardino, Ricardo Leao, Rui Henrique, Luis Campos Pinheiro, Prashant Kumar, Prashanth Suravajhala, Hans Christian Beck, Ana Sofia Carvalho, Rune Matthiesen
Summary: The study explores possible strategies for urinary biomarker discovery, especially focused on proteome of urine EVs for PCa. Through reanalysis of proteomics data and network analysis, the findings suggest that urinary EVs may provide potential biomarkers with higher protein coverage compared to other sample sources and may fully characterize the heterogeneity of PCa.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Immunology
Boris Vidal-Veuthey, Damariz Gonzalez, Juan P. Cardenas
Summary: This article reviews the literature on the functions of secreted proteins from the gut microbiota and discusses their impact on host physiology and health, as well as how these effects can be utilized for the development of postbiotic products.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Microbiology
Sakaorat Lertjuthaporn, Jinjuta Somkird, Kittima Lekmanee, Anyapat Atipimonpat, Kasama Sukapirom, Hathai Sawasdipokin, Supathra Tiewcharoen, Kovit Pattanapanyasat, Ladawan Khowawisetsut
Summary: This study examines the role of N. fowleri-derived extracellular vesicles (Nf-EVs) in host-pathogen interactions. The results show that Nf-EVs can induce macrophage responses by upregulating the expression of costimulatory molecules and cytokine production, but have no effect on macrophage apoptosis. These findings reveal the potential role of Nf-EVs in mediating host immune cell activation and disease pathogenesis.
Review
Nutrition & Dietetics
Elena Gonzalez-Lozano, Jorge Garcia-Garcia, Julio Galvez, Laura Hidalgo-Garcia, Alba Rodriguez-Nogales, Maria Elena Rodriguez-Cabezas, Manuel Sanchez
Summary: Lactobacillus probiotics have beneficial effects on host health and various pathological conditions, especially in improving inflammatory bowel disease (IBD) and regulating the immune system. Extracellular vesicles (EVs) derived from probiotics play a key role in these interactions and have been proposed as a safe alternative to live bacteria for vulnerable patients. This review provides an updated overview of EVs from different Lactobacillus strains and their potential therapeutic applications.
Article
Immunology
Kritsada Pruksaphon, Artid Amsri, Patcharin Thammasit, Joshua D. Nosanchuk, Sirida Youngchim
Summary: Pathogenic eukaryotes, such as fungi, release extracellular vesicles (EVs) that are composed of various bioactive components. These EVs contain virulence-associated molecules and play an important role in disease pathogenesis. Studies on Evans of Talaromyces marneffei show that they contain important virulence factors, stimulate macrophage activation, and enhance antimicrobial activity.
FRONTIERS IN IMMUNOLOGY
(2023)
Review
Cell Biology
Lana Ginini, Salem Billan, Eran Fridman, Ziv Gil
Summary: Extracellular vesicles (EVs) are crucial heterogenous lipid structures secreted by cells for cell-to-cell communication. EVs primarily enter cells through endocytosis, a process that may depend on proteins and lipids on the surfaces of both vesicles and target cells.
Article
Clinical Neurology
Victor Bodart-Santos, Lisandra S. Pinheiro, Almir J. da Silva-Junior, Rudimar L. Froza, Rosemary Ahrens, Rafaella A. Goncalves, Mayara M. Andrade, Yan Chen, Carolina de Lima Alcantara, Lea T. Grinberg, Renata E. P. Leite, Sergio T. Ferreira, Paul E. Fraser, Fernanda G. De Felice
Summary: The study found that extracellular vesicles (EVs) in Alzheimer's disease (AD) and frontotemporal dementia (FTD) not only contribute to the spread of neuropathology, but also have an impact on behavioral outcomes associated with AD. Memory tests were conducted by injecting EVs isolated from post mortem brain tissue from control, AD, or FTD donors, as well as from APP/PS1 mice, into the hippocampi of wild-type or a humanized Tau mouse model. The results showed that AD-EVs and FTD-EVs carry Tau protein, have altered protein composition related to synapse regulation and transmission, and induce memory impairment.
ALZHEIMERS & DEMENTIA
(2023)
Article
Respiratory System
Alireza Javadi, Masoud Shamaei, Payam Tabarsi, Masoumeh Nomani, Mohammad Varahram, Bahram Kazemi
Summary: This study evaluates the effects of extracellular vesicles (EVs) from serum samples of mycobacteria-infected patients on THP-1 monocytes and PBMC cells. The results show that the isolated EVs from serum samples of patients with mycobacterial infection accelerated cell death. This suggests that they might not be considered as an optimal tool for developing drug delivery and vaccine against tuberculosis.
BMC PULMONARY MEDICINE
(2022)
Article
Biochemical Research Methods
Zivile Useckaite, Lauren A. Newman, Ashley M. Hopkins, Sonja Klebe, Alex D. Colella, Nusha Chegeni, Ruth Williams, Michael J. Sorich, A. David Rodrigues, Tim K. Chataway, Andrew Rowland
Summary: The study aimed to compare the proteomic profiles of extracellular vesicles (EVs) isolated from liver tissue and the extracellular space. The results showed the potential of liver-derived EVs as a liquid biopsy for understanding the functional status of the liver.
Article
Cell Biology
Rosanne E. Veerman, Loes Teeuwen, Paulo Czarnewski, Gozde Gucluler Akpinar, AnnSofi Sandberg, Xiaofang Cao, Maria Pernemalm, Lukas M. Orre, Susanne Gabrielsson, Maria Eldh
Summary: The method for isolating EVs impacts sample type, interest in EV subtypes, sample volume, and budget, and there is no one-size-fits-all method for all studies.
JOURNAL OF EXTRACELLULAR VESICLES
(2021)
Article
Biochemistry & Molecular Biology
Pei Jiang, Xiangyu Ma, Shanying Han, Leyao Ma, Jingru Ai, Leilei Wu, Yuan Zhang, Hairong Xiao, Mengyao Tian, W. Andy Tao, Shasha Zhang, Renjie Chai
Summary: This study discovers the existence of sEVs in the cochlea and reveals their importance in the normal development of the auditory system.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Article
Biotechnology & Applied Microbiology
Vanessa Croatti, Carola Parolin, Barbara Giordani, Claudio Foschi, Stefano Fedi, Beatrice Vitali
Summary: Extracellular vesicles (EVs) released by lactobacilli can enhance the adhesion of beneficial lactobacilli while reducing the adhesion of pathogens in the vaginal microenvironment. This study provides new insights into the development of postbiotics from vaginal strains to maintain microbial balance and promote women's health.
MICROBIAL CELL FACTORIES
(2022)
Review
Pharmacology & Pharmacy
Marta Bryk, Elzbieta Karnas, Jakub Mlost, Ewa Zuba-Surma, Katarzyna Starowicz
Summary: MSCs, as multipotent progenitor cells derived from mesoderm, have been extensively studied for their regenerative properties, including the release of EVs which play a role in their functions. They regulate various processes in target cells and show potential applications in pain treatment and other diseases.
BRITISH JOURNAL OF PHARMACOLOGY
(2022)
Article
Cell Biology
Anna Labedz-Maslowska, Agnieszka Szkaradek, Tomasz Mierzwinski, Zbigniew Madeja, Ewa Zuba-Surma
Summary: This study optimized the protocols for processing and ex vivo expansion of adipose tissue-derived mesenchymal stem cells (AT-MSCs) for the development of an Advanced Therapy Medicinal Product (ATMP) for human use. Critical process parameters were identified and examined to ensure the quality of the final product containing AT-MSCs.
Article
Biochemistry & Molecular Biology
Magdalena Wytrwal-Sarna, Malgorzata Sekula-Stryjewska, Agata Pomorska, Ewa Oclon, Katarzyna Gajos, Michal Sarna, Ewa Zuba-Surma, Andrzej Bernasik, Krzysztof Szczubialka
Summary: Recombinant human bone morphogenetic protein-2 plays a key role in stem cell response, but its supra-physiological levels cause adverse effects. This study investigates the binding of rhBMP-2 to ultrathin-multilayers and the effects on cell adhesion and osteogenesis marker expression.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Neurosciences
Debarati Bhattacharya, Jolanta Gorska-Andrzejak, Terence Al L. Abaquita, Elzbieta Pyza
Summary: A single adenosine receptor gene (dAdoR) in Drosophila melanogaster plays a role in the regulation of fitness through communication between neurons and glial cells. Overexpression or silencing of dAdoR in different cell types affects the survival rate, lifespan, climbing ability, and sleep patterns of the flies, depending on the age. Higher dAdoR levels in retina photoreceptors, neurons, and glial cells negatively influence survival and lifespan, while excess dAdoR in neurons improves climbing ability in older flies. Silencing of dAdoR decreases lifespan but increases survival rate in young flies. Silencing also affects the daily pattern of the presynaptic protein BRP, particularly when dAdoR expression is reduced in glial cells.
EXPERIMENTAL BRAIN RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Magdalena Wytrwal, Malgorzata Sekula-Stryjewska, Agata Pomorska, Ewa Oclon, Ewa Zuba-Surma, Szczepan Zapotoczny, Krzysztof Szczubialka
Summary: This study investigated the covalent binding of recombinant human bone morphogenetic protein-2 and -7 in stem cells and its effects on cell adhesion, migration, and osteogenesis. The presence of both proteins enhanced cell adhesion but limited cell motility, while the presence of single proteins promoted cell migration activity. Additionally, the coexistence of the proteins significantly increased early osteogenic marker expression.
Article
Microbiology
Justyna Karkowska-Kuleta, Kamila Kulig, Grazyna Bras, Karolina Stelmaszczyk, Magdalena Surowiec, Andrzej Kozik, Elzbieta Karnas, Olga Barczyk-Woznicka, Ewa Zuba-Surma, Elzbieta Pyza, Maria Rapala-Kozik
Summary: The involvement of Candida albicans biofilm-derived extracellular vesicles (EVs) in biofilm stability, metabolic activity, and cell viability, as well as their effect on antifungal drugs and proteolytic activity, were verified in this study.
Article
Cell & Tissue Engineering
Katarzyna Kmiotek-Wasylewska, Sylwia Bobis-Wozowicz, Elzbieta Karnas, Monika Orpel, Olga Woznicka, Zbigniew Madeja, Buddhadeb Dawn, Ewa K. Zuba-Surma
Summary: This study investigates the biological impact of stem cell-derived extracellular vesicles enriched in miR-1 and miR-199a on cardiac fibroblasts (CFs) and the underlying molecular mechanisms.
STEM CELL REVIEWS AND REPORTS
(2023)
Article
Biochemistry & Molecular Biology
Maria M. Guzewska, Krzysztof J. Witek, Elzbieta Karnas, Michal Rawski, Ewa Zuba-Surma, Monika M. Kaczmarek
Summary: Intercellular communication at the embryo-maternal interface plays a crucial role in ensuring cooperation between different cell types. Extracellular vesicles (EVs) are potent mediators of this communication by transferring miRNAs to recipient cells. A recent study reveals the impact of embryonic signals, including miRNAs, on EV-mediated cell-to-cell communication at the maternal side. The study demonstrates the regulatory mechanism of the miR-125b-5p ESCRT-mediated EV biogenesis pathway and the secretion of EVs by trophoblasts during implantation. The study further shows that miR-125b-5p modulates EV biogenesis and trafficking, specifically impacting the ESCRT-II complex and EV trafficking in porcine trophoblast cells.
Article
Biochemistry & Molecular Biology
Bernadetta Bilska, Urszula Godlewska, Milena Damulewicz, Krzysztof Murzyn, Mateusz Kwitniewski, Joanna Cichy, Elzbieta Pyza
Summary: The study found that the MFF-p1 peptide from Drosophila melanogaster possesses antimicrobial properties, showing strong inhibitory effects on E. coli and B. subtilis, and its expression is regulated by exposure to bacteria and the circadian clock.
CURRENT ISSUES IN MOLECULAR BIOLOGY
(2022)