Article
Biochemistry & Molecular Biology
Taro Yanai, Yukino Takahashi, Eri Katsumura, Naoki Sakai, Kohei Takeshita, Riki Imaizumi, Hiroaki Matsuura, Shuntaro Hongo, Toshiyuki Waki, Seiji Takahashi, Masaki Yamamoto, Kunishige Kataoka, Toru Nakayama, Satoshi Yamashita
Summary: The crystal structure of PSTG1 was determined, revealing the presence of an active site in domain 1 and an additional domain 4 that may play a role in substrate recognition. The study suggests that domain 4 could be a potential target for further investigation on the aglycone recognition mechanism of PSTG1 and for engineering the enzyme to improve sesaminol production efficiency.
JOURNAL OF BIOCHEMISTRY
(2023)
Article
Food Science & Technology
Kuo-Ching Jan, Tzu-Yi Wang, Lucy Sun Hwang, Mohsen Gavahian
Summary: This study investigated the metabolism of sesaminol triglucoside (STG) by microorganisms in swine-fed diets supplemented with either probiotics or antibiotics. It was found that probiotics helped with the hydrolysis of STG and generated metabolite 2-hydroxymethyl sesaminol-tetrahydrofuran (ST-2), while antibiotics had no such effect. The bacteria that converted sesaminol to ST-2 were found to be oxygen-sensitive, suggesting they were anaerobes. Lactobacillus and Bifidobacterium were more prevalent in probiotic-fed swine feces, and both groups of swine had a mix of beneficial and harmful bacteria.
QUALITY ASSURANCE AND SAFETY OF CROPS & FOODS
(2022)
Article
Biotechnology & Applied Microbiology
Tingting Long, Peng Zhang, Jingze Yu, Yushan Gao, Xiaoqin Ran, Yonghao Li
Summary: By adding miglitol to the fermentation medium of T. reesei, the cellulase titer increased more than two-fold. This method has potential in stimulating cellulase production and provides a promising system for T. reesei protein expression.
FERMENTATION-BASEL
(2022)
Article
Microbiology
Anke Chen, Dan Wang, Rui Ji, Jixi Li, Shaohua Gu, Rong Tang, Chaoneng Ji
Summary: In this study, a thermostable beta-glucosidase (TsBGL) was isolated and characterized from the Thermoprotei Thermofilum sp. ex4484_79, exhibiting high activity and specific activity at elevated temperatures and pH levels. The crystal structure of TsBGL reveals a classical (alpha/beta)(8)-barrel catalytic domain, with catalytic sites including Glu210 and Glu414. TsBGL shows promise for potential industrial applications with its high thermostability and catalytic activity.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Soil Science
Rachel C. Daughtridge, Yuhei Nakayama, Andrew J. Margenot
Summary: This study evaluated the abiotic hydrolysis of different substrates under various conditions, finding that alkalinization method and substrate type had significant impacts on the extent of abiotic hydrolysis. The study also highlighted the occurrence of appreciable abiotic hydrolysis during the incubation period.
SOIL BIOLOGY & BIOCHEMISTRY
(2021)
Article
Biotechnology & Applied Microbiology
Lu Mou, Runze Pan, Yansong Liu, Wankui Jiang, Wenming Zhang, Yujia Jiang, Fengxue Xin, Min Jiang
Summary: This study successfully isolated a fungus with efficient lignocellulose degradation capability, named T. asperellum LYS1, and found that it showed remarkable performance in degrading biomass rich in hemicellulose.
ENZYME AND MICROBIAL TECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Custan G. Fernandes, Sneha C. Sawant, Tejal A. Mule, Vishwanath S. Khadye, Arvind M. Lali, Annamma A. Odaneth
Summary: This study investigated the influence of beta-glucosidases (BGs) on cellulose hydrolysis, with results showing that commercial cellulase preparations are low in BG and yield below 60% in 12 hours, but synergistic effects were observed when blended with BGs, leading to improved hydrolysis efficiency.
PROCESS BIOCHEMISTRY
(2022)
Article
Plant Sciences
Marino Pedro Reyes-Martin, Emilia Fernandez-Ondono, Irene Ortiz-Bernad, Maria Manuela Abreu
Summary: Agricultural soil quality has been extensively discussed in recent decades. This study focused on three olive grove areas in Portugal with different management techniques, aiming to assess the influence of these techniques on soil quality. The results showed that the concentrations of organic carbon and total nitrogen were highest in the intensive olive grove in Lisbon, while Ca, Mg, Na, and K were the main differences between Lisbon and the other two groves in Santarem. Enzymatic activities were found to be related to various soil concentrations. Soil management and agricultural practices were identified as determining factors for enzymatic activities in the olive groves.
Article
Microbiology
Ai-Ping Pang, Haiyan Wang, Yongsheng Luo, Zihuayuan Yang, Zhiyu Liu, Zhao Wang, Bingzhi Li, Song Yang, Zhihua Zhou, Xiaolin Lu, Fu-Gen Wu, Zuhong Lu, Fengming Lin
Summary: This study revealed that overexpression of cel3b or cel3g significantly increased extracellular beta-glucosidase production, while overexpression of cel1b inhibited cellulase production. Four cellular distribution patterns of beta-glucosidases in T. reesei were identified, providing insight into their role in cellulase production and cell function. Furthermore, the study elucidated the secretion pathways of different beta-glucosidases, advancing understanding of their properties in filamentous fungi.
Article
Genetics & Heredity
Prajeesh Kooloth-Valappil, Meera Christopher, AthiraRaj Sreeja-Raju, Reshma M. Mathew, Rajasree Kuni-Parambil, Amith Abraham, Meena Sankar, Ashok Pandey, Rajeev K. Sukumaran
Summary: The draft genome sequence of the rare fungus Aspergillus unguis NII 08,123, producing glucose-tolerant beta glucosidase (GT-BGL), was generated using Next Generation Sequencing (NGS). The genome size was estimated to be 37.1 Mb, with 15,161 predicted proteins. Analysis of CAZyme genes revealed differences in lignocellulose degrading enzyme distribution between A. unguis and Trichoderma reesei, potentially due to tight regulation of cellulolytic machinery in the former. The fungus contained 403 predicted CAZymes, including various classes such as glycoside hydrolases, carbohydrate esterases, glycosyl transferases, polysaccharide lyases, and genes with auxiliary activities.
FUNGAL GENETICS AND BIOLOGY
(2021)
Review
Biotechnology & Applied Microbiology
Su Yan, Yan Xu, Xiao-Wei Yu
Summary: The review summarizes recent progress in cellulase production of the filamentous fungus Trichoderma reesei, highlighting key factors such as carbon source-dependent induction, transcriptional regulation, and protein assembly. These findings shed light on potential directions for further engineering.
BIORESOURCES AND BIOPROCESSING
(2021)
Article
Green & Sustainable Science & Technology
Rossella Vadala, Giovanna Lo Vecchio, Rossana Rando, Michelangelo Leonardi, Nicola Cicero, Rosaria Costa
Summary: The present study developed a cost-effective, energy-saving, and ecofriendly methodology for producing ethanol from citrus fruit waste. This involved pretreating the waste through physical means, followed by enzymatic hydrolysis and fermentation. The process resulted in the production of 40.1 g/L ethanol in 48 hours, and an additional fed batch step increased the ethanol yield to 52.3 g/L. The study successfully achieved its objectives of sustainability.
Article
Chemistry, Multidisciplinary
Manuel Scherer, Andres G. Santana, Kyle Robinson, Steven Zhou, Hermen S. Overkleeft, Lorne Clarke, Stephen G. Withers
Summary: This study introduces a new class of transient GCase inactivators with improved stability in aqueous media, and validates through cellular research that treatment with these compounds can restore catalytic activity in Gaucher cells.
Article
Nutrition & Dietetics
Su-Jin Jung, Eun-Soo Jung, Ki-Chan Ha, Hyang-Im Baek, Yu-Kyung Park, Soog-Kyoung Han, Soo-Wan Chae, Seung-Ok Lee, Young-Chul Chung
Summary: The study suggests that supplementation with sesame oil cake extract can improve verbal memory abilities and lower plasma beta-amyloid levels in older adults with memory impairment.
Article
Biotechnology & Applied Microbiology
Zekang Wang, Haiyan Chen, Yuyue Qin, Tianqing Lan
Summary: In this study, Fenton oxidation was used to modify lignin and investigate its effect on immobilized beta-glucosidase (beta-GL). The results showed that Fenton oxidation significantly enhanced the activity and stability of immobilized beta-GL. This was attributed to increased interactions between lignin and beta-GL, as well as changes in the chemical structure of lignin. This research contributes to a better understanding of Fenton lignin oxidation and expands the applications of lignin in enzyme immobilization.
JOURNAL OF BIOTECHNOLOGY
(2023)
Article
Food Science & Technology
Husnain Raza, Kashif Ameer, Farah Zaaboul, Muhammad Shoaib, Chang-Cheng Zhao, Barkat Ali, Muhammad Talha Shahzad, Muhammad Abid, Xiaofeng Ren, Lianfu Zhang
Summary: This study evaluated the effects of ball-milled roasted chickpea powder on the physicochemical, rheological, and sensory properties of supplemented yogurt. The results showed that the pH decreased and color became brighter and more yellow with increasing powder concentration. The supplemented samples had higher firmness and lower syneresis compared to the control. The addition of more than 3% roasted chickpea resulted in smoother particle dispersion and lower shear-thinning. The study suggests that roasted chickpea powder can be used as a functional ingredient in yogurt production.
FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Food Science & Technology
Longhua Ma, Qingran Meng, Feng Chen, Wenjie Gao
Summary: The volatile compounds in Chinese Zhizhonghe Wujiapi (WJP) medicinal liquor were extracted and identified using two extraction methods. A total of 123 compounds, including 67 aroma-active compounds, were identified. Reconstitution experiments confirmed the importance of key aroma-active compounds in determining the aroma profile of the liquor.
JOURNAL OF FOOD SCIENCE
(2022)
Article
Polymer Science
Yulei Tian, Xin Huang, Ying Cheng, Yunwei Niu, Qingran Meng, Jiajia Ma, Yi Zhao, Xingran Kou, Qinfei Ke
Summary: This study focuses on the preparation of self-adhesive microcapsules for multiple recyclable adhesion and de-adhesion on cotton fabrics through the use of Fe3+ and EDTA. The microcapsules can achieve controlled adhesion by coordination with cotton fabrics and disruption of this binding by EDTA, offering new ideas for functionalization and diversification of textiles.
JOURNAL OF APPLIED POLYMER SCIENCE
(2022)
Review
Polymer Science
Dan Yuan, Xin Huang, Qingran Meng, Jiajia Ma, Yi Zhao, Qinfei Ke, Xingran Kou
Summary: Polymer gels, particularly zein-based gel networks, have gained significant attention in the fields of food science and biomedicine. Zein, a protein derived from maize, has unique film-forming properties and can be combined with other substances to form gel networks for various applications such as encapsulation and delivery of active substances. This review explores the construction, performance, and potential value of zein gel networks, as well as prospects for future development and application.
EUROPEAN POLYMER JOURNAL
(2022)
Article
Food Science & Technology
Jian Sun, Liangge Sun, Xinxiang Chen, Husnain Raza, Gangshan Wu, Qiufang Liang, Xiaofeng Ren
Summary: The study investigated the impact of ultrasonic-assisted alpha-amylase hydrolysis on the structure and physicochemical properties of arrowhead-derived type 3 resistant starch (RS3). The yield of RS3 significantly increased by 65.64% to reach 17.21% after ultrasonic treatment. RS3-UAE demonstrated improved gelatinization temperature, relative crystallinity, enthalpy, and 1047/1022 values compared to RS3-E. RS3-UAE also exhibited higher solubility, transparency, water absorption capacity, and swelling power at 70℃, as well as a more regular shape, smoother surface, higher crystallinity, stable double helix structure, and more ordered and denser structure.
JOURNAL OF FOOD QUALITY
(2023)
Article
Plant Sciences
Wenjie Gao, Qingran Meng, Xiang Wang, Feng Chen, Yunwei Zhou, Miao He
Summary: In this study, a transcription factor CiMYC2 was discovered in chrysanthemum, and its overexpression in transgenic tobacco resulted in increased terpenoid content and the production of novel terpenoid compounds. These findings provide a theoretical basis for creating high terpene aromatic materials and cultivating new varieties of aromatic chrysanthemum using transgenic engineering technology.
JOURNAL OF PLANT GROWTH REGULATION
(2023)
Article
Biochemistry & Molecular Biology
Xingran Kou, Xinping Zhang, Ying Cheng, Miao Yu, Qingran Meng, Qinfei Ke
Summary: The aim of this study is to find the best solution for preventing agglomeration in spray-dried microcapsules powder. Among the tested anticaking agents, mannitol was found to be the most effective one. Microcapsules with a Xanthium-type shape containing 12% mannitol showed improved powder flowability, lower hygroscopicity, and good anticaking properties. The mechanisms behind the anticaking effects involve increasing the glass transition temperature for calcium stearate, magnesium stearate, and silicon dioxide, while mannitol forms a crystal outer-layer to prevent the hydroxyl group from combining with water molecules.
Article
Plant Sciences
Wenjie Gao, Qingran Meng, Xiang Wang, Feng Chen, Miao He, Yunwei Zhou
Summary: The cloning and analysis of two key enzyme genes from Chrysanthemum indicum var. aromaticum showed that their overexpression can improve the growth traits and terpenoid metabolism of the plant.
PLANT GROWTH REGULATION
(2023)
Article
Plant Sciences
Lifei Chen, Bin Xia, Ziwei Li, Xiaowei Liu, Yun Bai, Yujia Yang, Wenjie Gao, Qingran Meng, Ning Xu, Ying Sun, Qiang Li, Liran Yue, Miao He, Yunwei Zhou
Summary: We presented a chromosome-scale genome assembly of Syringa oblata and identified important genes involved in its ornamental traits. Additionally, we identified and analyzed the major floral volatiles formation genes in Syringa oblata. These results provide important references for mechanistic studies on the main ornamental traits and molecular breeding in Syringa oblata.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Husnain Raza, Kashif Ameer, Xiaofeng Ren, Yuxuan Liu, Lixin Kang, Qiufang Liang, Tao Guo, Haile Ma, Mingfu Wang
Summary: This study investigated the multi-scale structural interactions between arrowhead starch (AS) and phenolic acids (such as FA and GA) to understand the mechanism of starch's anti-digestion effects. The synergistic heat-ultrasound treatment (HUT) significantly improved the dispersion of phenolic acids in the starch, with GA showing a higher complexation index than FA. XRD analysis revealed the formation of an inclusion complex for GA, while peak intensities decreased for FA after HT and HUT. FTIR and Raman spectroscopy provided further insights into the structural changes within the samples.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Food Science & Technology
Xingran Kou, Yaqi Zhang, Dongdong Su, Hui Wang, Xin Huang, Yunwei Niu, Qinfei Ke, Zuobing Xiao, Qingran Meng
Summary: This study characterized the inclusion complexes (ICs) of α-cyclodextrin (α-CD) with 13 aroma compounds and explored the host-guest interaction and stabilization mechanisms using various techniques. The results showed that aroma compounds weakened or disappeared after inclusion, and all ICs were crystalline. The molecular dynamics simulations revealed the preferential entry of the benzene ring and methyl group/double bond of α-terpineol into the hydrophobic cavity. The study aimed to provide a theoretical basis for the stable and controllable preparation of aroma embedding systems.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2023)
Article
Nutrition & Dietetics
Xingran Kou, Xinping Zhang, Qinfei Ke, Qingran Meng
Summary: In this study, beta-cyclodextrin (beta-CD) and ginger oil (GO) were used to prepare Pickering emulsion without an exogenous emulsifier. The obtained emulsion exhibited flocculation and had rough droplet surfaces with lamellar particles. By investigating the formation process and evolution of GO/beta-CD self-assembly, the formation process and stabilization mechanism of the beta-CD-stabilized GO Pickering emulsion were clarified preliminarily.
FRONTIERS IN NUTRITION
(2023)
Article
Chemistry, Applied
Arif Rashid, Abdul Qayum, Qiufang Liang, Lixin Kang, Husnain Raza, Zhuzhong Chi, Runhao Chi, Xiaofeng Ren, Haile Ma
Summary: Ultrasound is a high-energy method that helps with homogenization and dispersion in cavitation. Nanoemulsions of curcumin and orange essential oil were prepared using ultrasound treatment at different times. The 10-minute ultrasound-treated nanoemulsions had the smallest droplet size, the best storage, and higher thermal stability. The pullulan-based film with ultrasound-assisted nanoemulsions showed improved water vapor permeability and moisture content, as well as the highest tensile strength and elongation at break. Structural analysis revealed enhanced H-bonding and more orderly molecular arrangement due to ultrasonic treatment, resulting in excellent bacteriostatic properties and prolonged shelf life for strawberry fruit.
Article
Agricultural Engineering
Qinfei Ke, Hui Wang, Xin Huang, Yunchong Zhang, Qingran Meng, Xingran Kou
Summary: This study investigated the use of vanillin as a cross-linking agent in gelatin and carboxymethylcellulose sodium complex coacervation system. Two different composite films were prepared with different addition methods of vanillin. The results showed that vanillin significantly affected the physicochemical and functional properties of the films, and could be used to strengthen protein-polysaccharide-based composite films.
INDUSTRIAL CROPS AND PRODUCTS
(2023)
Review
Biochemistry & Molecular Biology
Qinfei Ke, Kangning Ma, Yunchong Zhang, Qingran Meng, Xin Huang, Xingran Kou
Summary: Electrospinning is a promising technique for producing biopolymer nanofibers. To improve their properties, strategies such as polymer blending, cross-linking agents, nanoparticles, and modification of biopolymers have been developed. Among these, antibacterial aroma compounds (AACs) from essential oils are widely used as bioactive components in biopolymer nanofibers to enhance their functionality. This review summarizes the antimicrobial activities and applications of AACs, and compares the effects of different electrospinning strategies on the properties and applications of nanofibers.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
