Rubisco Function, Evolution, and Engineering

Machine learning-aided engineering of hydrolases for PET depolymerization

(2022) Hongyuan Lu et al.   NATURE

Rubisco regulation in response to altered carbon status in the cyanobacterium Synechococcus elongatus PCC 7942

(2022) Amit K Singh et al.   PLANT PHYSIOLOGY

The small subunit of Rubisco and its potential as an engineering target

(2022) Yuwei Mao et al.   JOURNAL OF EXPERIMENTAL BOTANY

The discovery of rubisco

(2022) Thomas D Sharkey   JOURNAL OF EXPERIMENTAL BOTANY

Predicting plant Rubisco kinetics from RbcL sequence data using machine learning

(2022) Wasim A Iqbal et al.   JOURNAL OF EXPERIMENTAL BOTANY

Evolution of increased complexity and specificity at the dawn of form I Rubiscos

(2022) Luca Schulz et al.   SCIENCE

Rubisco, the imperfect winner: it’s all about the base

(2022) Murray R Badger et al.   JOURNAL OF EXPERIMENTAL BOTANY

Synthetic Enzyme‐Catalyzed CO 2 Fixation Reactions

(2021) Godwin A. Aleku et al.   ChemSusChem

Deep diversification of an AAV capsid protein by machine learning

(2021) Drew H. Bryant et al.   NATURE BIOTECHNOLOGY

Rubisco adaptation is more limited by phylogenetic constraint than by catalytic trade-off

(2021) Jacques W Bouvier et al.   MOLECULAR BIOLOGY AND EVOLUTION

On the evolution of chaperones and cochaperones and the expansion of proteomes across the Tree of Life

(2021) Mathieu E. Rebeaud et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Rubisco substitutions predicted to enhance crop performance through carbon uptake modelling

(2021) Wasim A Iqbal et al.   JOURNAL OF EXPERIMENTAL BOTANY

Improving photosynthesis through the enhancement of Rubisco carboxylation capacity

(2021) Concepción Iñiguez et al.   BIOCHEMICAL SOCIETY TRANSACTIONS

Rubisco catalytic adaptation is mostly driven by photosynthetic conditions – Not by phylogenetic constraints

(2021) Guillaume Tcherkez et al.   JOURNAL OF PLANT PHYSIOLOGY

Multivalent interactions between CsoS2 and Rubisco mediate α-carboxysome formation

(2020) Luke M. Oltrogge et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Prospects for Engineering Biophysical CO2 Concentrating Mechanisms into Land Plants to Enhance Yields

(2020) Jessica H. Hennacy et al.   Annual Review of Plant Biology

Highly active rubiscos discovered by systematic interrogation of natural sequence diversity

(2020) Dan Davidi et al.   EMBO JOURNAL

Modifying Plant Photosynthesis and Growth via Simultaneous Chloroplast Transformation of Rubisco Large and Small Subunits

(2020) Elena Martin-Avila et al.   PLANT CELL

Ribulose 1,5-bisphosphate carboxylase/oxygenase activates O2 by electron transfer

(2020) Camille Bathellier et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

The dependency of red Rubisco on its cognate activase for enhancing plant photosynthesis and growth

(2020) Laura H. Gunn et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Functional reconstitution of a bacterial CO2 concentrating mechanism in E. coli

(2020) Avi I Flamholz et al.   eLife

Awakening a latent carbon fixation cycle in Escherichia coli

(2020) Ari Satanowski et al.   Nature Communications

Synthetic glycolate metabolism pathways stimulate crop growth and productivity in the field

(2019) Paul F. South et al.   SCIENCE

The global mass and average rate of rubisco

(2019) Yinon M. Bar-On et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Rubisco condensate formation by CcmM in β-carboxysome biogenesis

(2019) H. Wang et al.   NATURE

Mechanism of Oxygenase-Pathway Reactions Catalyzed by Rubisco from Large-Scale Kohn–Sham Density Functional Calculations

(2019) Babu Kannappan et al.   JOURNAL OF PHYSICAL CHEMISTRY B

Revisiting Trade-offs between Rubisco Kinetic Parameters

(2019) Avi I. Flamholz et al.   BIOCHEMISTRY

Selection of Cyanobacterial (Synechococcus sp. Strain PCC 6301) RubisCO Variants with Improved Functional Properties That Confer Enhanced CO2-Dependent Growth of Rhodobacter capsulatus, a Photosynthetic Bacterium

(2019) Sriram Satagopan et al.   mBio

Structural Perturbations of Rhodopseudomonas palustris Form II RuBisCO Mutant Enzymes That Affect CO2 Fixation

(2019) Sriram Satagopan et al.   BIOCHEMISTRY

Design and engineering of E. coli metabolic sensor strains with a wide sensitivity range for glycerate

(2019) Selçuk Aslan et al.   METABOLIC ENGINEERING

Form III RubisCO-mediated transaldolase variant of the Calvin cycle in a chemolithoautotrophic bacterium

(2019) Evgenii N. Frolov et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Increasing Photosynthesis: Unlikely Solution For World Food Problem

(2019) Thomas R. Sinclair et al.   TRENDS IN PLANT SCIENCE

Conversion of Escherichia coli to Generate All Biomass Carbon from CO2

(2019) Shmuel Gleizer et al.   CELL

Directed Evolution of an Improved Rubisco; In Vitro Analyses to Decipher Fact from Fiction

(2019) Yu Zhou et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Response: Commentary: Directions for Optimization of Photosynthetic Carbon Fixation: RuBisCO’s Efficiency May Not Be So Constrained After All

(2019) Peter L. Cummins et al.   Frontiers in Plant Science

Evolutionary trends in RuBisCO kinetics and their co‐evolution with CO 2 concentrating mechanisms

(2019) Concepción Iñiguez et al.   PLANT JOURNAL

A short history of RubisCO: the rise and fall (?) of Nature's predominant CO 2 fixing enzyme

(2018) Tobias J Erb et al.   CURRENT OPINION IN BIOTECHNOLOGY

Structural and functional analyses of Rubisco from arctic diatom species reveal unusual posttranslational modifications

(2018) Karin Valegård et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Revised mechanism of carboxylation of ribulose-1,5-biphosphate by rubisco from large scale quantum chemical calculations

(2018) Peter L. Cummins et al.   JOURNAL OF COMPUTATIONAL CHEMISTRY

Functional assignment of multiple catabolic pathways for d-apiose

(2018) Michael S. Carter et al.   Nature Chemical Biology

Rubisco is not really so bad

(2018) Camille Bathellier et al.   PLANT CELL AND ENVIRONMENT

Commentary: Directions for Optimization of Photosynthetic Carbon Fixation: RuBisCO's Efficiency May Not Be So Constrained After All

(2018) Guillaume G. Tcherkez et al.   Frontiers in Plant Science

Carboxysome encapsulation of the CO2-fixing enzyme Rubisco in tobacco chloroplasts

(2018) Benedict M. Long et al.   Nature Communications

Lateral Gene Transfer Shapes the Distribution of RuBisCO among Candidate Phyla Radiation Bacteria and DPANN Archaea

(2018) Alexander L Jaffe et al.   MOLECULAR BIOLOGY AND EVOLUTION

Design and in vitro realization of carbon-conserving photorespiration

(2018) Devin L. Trudeau et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

An improvedEscherichia coliscreen for Rubisco identifies a protein–protein interface that can enhance CO2-fixation kinetics

(2017) Robert H. Wilson et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Mechanism of Enzyme Repair by the AAA + Chaperone Rubisco Activase

(2017) Javaid Y. Bhat et al.   MOLECULAR CELL

Increased SBPase activity improves photosynthesis and grain yield in wheat grown in greenhouse conditions

(2017) Steven M. Driever et al.   PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES

Plant RuBisCo assembly inE. coliwith five chloroplast chaperones including BSD2

(2017) H. Aigner et al.   SCIENCE

A RuBisCO-mediated carbon metabolic pathway in methanogenic archaea

(2017) Takunari Kono et al.   Nature Communications

Evolution of Oxygenic Photosynthesis

(2016) Woodward W. Fischer et al.   Annual Review of Earth and Planetary Sciences

Sugar Synthesis from CO2 in Escherichia coli

(2016) Niv Antonovsky et al.   CELL

Functional metagenomic selection of ribulose 1, 5-bisphosphate carboxylase/oxygenase from uncultivated bacteria

(2016) Vanessa A. Varaljay et al.   ENVIRONMENTAL MICROBIOLOGY

RubisCO selection using the vigorously aerobic and metabolically versatile bacterium Ralstonia eutropha

(2016) Sriram Satagopan et al.   FEBS Journal

RubisCO of a nucleoside pathway known from Archaea is found in diverse uncultivated phyla in bacteria

(2016) Kelly C Wrighton et al.   ISME Journal

A synthetic pathway for the fixation of carbon dioxide in vitro

(2016) Thomas Schwander et al.   SCIENCE

Evolving Methanococcoides burtonii archaeal Rubisco for improved photosynthesis and plant growth

(2016) Robert H. Wilson et al.   Scientific Reports

In VivoStudies inRhodospirillum rubrumIndicate That Ribulose-1,5-bisphosphate Carboxylase/Oxygenase (Rubisco) Catalyzes Two Obligatorily Required and Physiologically Significant Reactions for Distinct Carbon and Sulfur Metabolic Pathways

(2015) Swati Dey et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Opposing effects of folding and assembly chaperones on evolvability of Rubisco

(2015) Paulo Durão et al.   Nature Chemical Biology

Transgenic tobacco plants with improved cyanobacterial Rubisco expression but no extra assembly factors grow at near wild-type rates if provided with elevated CO2

(2015) Alessandro Occhialini et al.   PLANT JOURNAL

Redesigning photosynthesis to sustainably meet global food and bioenergy demand

(2015) Donald R. Ort et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Accuracy-rate tradeoffs: how do enzymes meet demands of selectivity and catalytic efficiency?

(2014) Dan S Tawfik   CURRENT OPINION IN CHEMICAL BIOLOGY

A function-based screen for seeking RubisCO active clones from metagenomes: novel enzymes influencing RubisCO activity

(2014) Stefanie Böhnke et al.   ISME Journal

Structure-Function Studies with the Unique Hexameric Form II Ribulose-1,5-bisphosphate Carboxylase/Oxygenase (Rubisco) fromRhodopseudomonas palustris

(2014) Sriram Satagopan et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

A faster Rubisco with potential to increase photosynthesis in crops

(2014) Myat T. Lin et al.   NATURE

Development of an activity-directed selection system enabled significant improvement of the carboxylation efficiency of Rubisco

(2014) Zhen Cai et al.   Protein & Cell

D2O Solvent Isotope Effects Suggest Uniform Energy Barriers in Ribulose-1,5-bisphosphate Carboxylase/Oxygenase Catalysis

(2013) Guillaume G. B. Tcherkez et al.   BIOCHEMISTRY

Modelling the reaction mechanism of ribulose-1,5-bisphosphate carboxylase/oxygenase and consequences for kinetic parameters

(2013) GUILLAUME TCHERKEZ   PLANT CELL AND ENVIRONMENT

Thermodynamic constraints shape the structure of carbon fixation pathways

(2012) Arren Bar-Even et al.   BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS

Engineering specialized metabolic pathways—is there a room for enzyme improvements?

(2012) Arren Bar-Even et al.   CURRENT OPINION IN BIOTECHNOLOGY

Structural mechanism of RuBisCO activation by carbamylation of the active site lysine

(2012) B. Stec   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Carboxylases in Natural and Synthetic Microbial Pathways

(2011) Tobias J. Erb   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

The Moderately Efficient Enzyme: Evolutionary and Physicochemical Trends Shaping Enzyme Parameters

(2011) Arren Bar-Even et al.   BIOCHEMISTRY

A survey of carbon fixation pathways through a quantitative lens

(2011) Arren Bar-Even et al.   JOURNAL OF EXPERIMENTAL BOTANY

S-nitrosylation: An emerging post-translational protein modification in plants

(2011) Jéremy Astier et al.   PLANT SCIENCE

Alternative Pathways of Carbon Dioxide Fixation: Insights into the Early Evolution of Life?

(2010) Georg Fuchs   Annual Review of Microbiology

Functional Prokaryotic RubisCO from an Oceanic Metagenomic Library

(2010) B. Witte et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Cross-species analysis traces adaptation of Rubisco toward optimality in a low-dimensional landscape

(2010) Yonatan Savir et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Design and analysis of synthetic carbon fixation pathways

(2010) A. Bar-Even et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Contributions of anoxygenic and oxygenic phototrophy and chemolithotrophy to carbon and oxygen fluxes in aquatic environments

(2009) JA Raven   AQUATIC MICROBIAL ECOLOGY

Rubisco Oligomers Composed of Linked Small and Large Subunits Assemble in Tobacco Plastids and Have Higher Affinities for CO2 and O2

(2009) S. M. Whitney et al.   PLANT PHYSIOLOGY

Multiple Rubisco forms in proteobacteria: their functional significance in relation to CO2 acquisition by the CBB cycle

(2008) Murray Ronald Badger et al.   JOURNAL OF EXPERIMENTAL BOTANY

The evolution of inorganic carbon concentrating mechanisms in photosynthesis

(2008) J. A Raven et al.   PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES

Directing the evolution of Rubisco and Rubisco activase: first impressions of a new tool for photosynthesis research

(2008) Oliver Mueller-Cajar et al.   PHOTOSYNTHESIS RESEARCH

Structure and function of Rubisco

(2008) Inger Andersson et al.   PLANT PHYSIOLOGY AND BIOCHEMISTRY