Article
Plant Sciences
Ilaria Chiocchio, Nerea Perez Andres, Redouan Adam Anaia, Nicole M. van Dam, Fredd Vergara
Summary: There are qualitative and quantitative differences in the steroidal glycosides profile between primary and adventitious roots of Solanum dulcamara, which can be used to evaluate the bioactivity of these molecules in belowground ecosystems.
Article
Biochemical Research Methods
Marketa Simkova, Lucie Kolatorova, Pavel Drasar, Jana Vitku
Summary: A robust and rapid LC-MS/MS method for the quantification of 32 steroid hormones in human plasma was developed. The method was validated for the analysis of C18-, C19-, and C21- steroids, demonstrating its applicability in physiological and pathophysiological conditions. This method will serve as a useful tool for investigating steroid-related diseases and can be expanded to analyze other biological matrices.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Isabelle Matias, Ilaria Belluomo, Pierre-Louis Raux, Monique Vallee
Summary: The emergence of metabolomics and quantification approaches has allowed for the discovery of new biomarkers for drug development. Tandem mass spectrometry is the preferred method for this, but it requires a specific validation process for both preclinical and clinical applications. Research on lipid mediators, specifically steroids and cannabinoids, has revealed a potential link to cannabis addiction and metabolism-related disorders. In this study, we developed GC-MS/MS and LC-MS/MS methods for routine quantification of targeted steroids and cannabinoids. Our methods showed satisfactory results for most validation conditions, although not all analytes met the acceptance criteria. Our validation process met the standards for GLP-qualified methods, allowing for routine analytical studies in preclinical and clinical settings.
Article
Biochemistry & Molecular Biology
Natalia Lomova, Natalia Dolgushina, Alisa Tokareva, Vitaly Chagovets, Natalia Starodubtseva, Ilya Kulikov, Gennady Sukhikh, Vladimir Frankevich
Summary: This study aimed to investigate the lipid profile of follicular fluid in COVID-19 patients and evaluate its impact on the outcomes of in vitro fertilization (IVF) procedures, in order to identify potential biomarkers for prognosis. Mass spectrometry was used to analyze follicular fluid samples from 237 patients, revealing significant changes in several lipids. The results suggest that lipid metabolism in follicular fluid may be associated with pregnancy occurrence and outcomes after severe COVID-19.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Medical Laboratory Technology
Philippe Massonnet, Elodie Grifnee, Jordi Farre-Segura, Justine Demeuse, Loreen Huyghebaert, Thomas Dubrowski, Patrice Dufour, Matthieu Schoumacher, Stephanie Peeters, Caroline Le Goff, Etienne Cavalier
Summary: Immunocapture is a mature method for sample preparation in quantitation of peptides and proteins. This review provides an overview of clinical applications of immunocapture methods, including protocols with and without enzymatic digestion. The advantages and limitations of these approaches are discussed, along with challenges related to mass spectrometer selection. The potential of these techniques in proteomics-based clinical medicine is demonstrated, paving the way for personalized medicine.
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
(2023)
Review
Medical Laboratory Technology
Stephanie Rankin-Turner, Liam M. Heaney
Summary: Mass spectrometry (MS) has become a powerful and versatile tool in clinical laboratory, with advancements in biomarker detection, protein measurement, therapeutic drug monitoring, and automation leading to potential revolution in clinical testing.
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
(2023)
Article
Biochemical Research Methods
Ashley Di Meo, Dorsa Sohaei, Ihor Batruch, Pantelis Alexandrou, Ioannis Prassas, Eleftherios P. Diamandis
Summary: The analysis utilized LC-MS/MS technology to profile the normal human proteome from 117 samples across 46 normal tissues and organs, quantifying 10,438 unique proteins. By combining data from biological fluid proteomics of healthy individuals, tandem mass spectra corresponding to 13,028 unique human protein-coding genes were generated, serving as an important complementary resource for future biomarker discovery.
JOURNAL OF PROTEOME RESEARCH
(2021)
Article
Biochemical Research Methods
Runmin Yang, Jingjing Ma, Shu Zhang, Yu Zheng, Lusheng Wang, Daming Zhu
Summary: This study introduces a new storage format called mzMD for MS datasets and presents an algorithm to query this format for summarizing a given data window. Experimental results demonstrate the high speed and stability of mzMD in retrieving high-quality data window summaries.
Article
Chemistry, Multidisciplinary
Hao Gu, Guoxin Dai, Siyu Liu, Yanbing Zhai, Qian Xu, Hongqiang Wu, Wei Xu
Summary: Liquid chromatography tandem mass spectrometry (LC-MS/MS) is an important analytical technique in clinical diagnostics. A compact LC-MS instrument was developed, consisting of an assembled liquid chromatograph and a miniature ion trap mass spectrometer. The use of LC improved the mixture analysis capability and detection sensitivity of the miniature mass spectrometer. This compact LC-MS instrument shows potential for clinical diagnosis.
CHINESE CHEMICAL LETTERS
(2023)
Article
Medical Laboratory Technology
Jordi Farre-Segura, Neus Fabregat-Cabello, Chiara Calaprice, Laurent Nyssen, Stephanie Peeters, Caroline Le Goff, Etienne Cavalier
Summary: A rapid and specific LC-MS/MS method for glucagon quantitation has been developed and validated, showing stability and accuracy in comparison to other immunoassay methods. This method could potentially establish a standardized approach for glucagon analysis in routine care settings.
CLINICA CHIMICA ACTA
(2021)
Article
Biochemical Research Methods
Armaghan Shafaei, Veronica Vamathevan, Jessica Pandohee, Nathan G. Lawler, David Broadhurst, Mary C. Boyce
Summary: This study presents a method for determining short-chain fatty acids in serum using high-resolution mass spectrometry and gas chromatography to identify isomeric interferences, which are overcome through a combination of chromatographic separation and judicious choice of MS fragment ion. The LODs and LOQs of SCFAs using this method were in the range 1 to 7 ng mL(-1) and 3 to 19 ng mL(-1), with a recovery ranging from 94 to 114% over the three concentration ranges tested.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Yuan-Chih Chen, Hsin-Yi Wu, Chih-Wei Chang, Pao-Chi Liao
Summary: Conjugation reactions play a critical role in human metabolism, but identifying conjugated metabolites is challenging. In this study, a strategy called PDMS2E-DIA was proposed for comprehensive profiling of the glucuronide-conjugated metabolome. It successfully identified a large number of glucuronide-conjugated metabolites in human urine samples.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Sakshi Gautam, Alireza Banazadeh, Byeong Gwan Cho, Mona Goli, Jieqiang Zhong, Yehia Mechref
Summary: Researchers successfully achieved efficient separation of glycan isomers using mesoporous graphitized carbon (MGC) material in a 1 cm-long column, showing reproducibility and stability in both capillary columns and packed nanospray emitters.
ANALYTICAL CHEMISTRY
(2021)
Article
Endocrinology & Metabolism
Yun Huang, Mixue Tu, Yuli Qian, Junyan Ma, Lifen Chen, Yifeng Liu, Yiqing Wu, Kai Chen, Juan Liu, Yanyun Ying, Yao Chen, Yinghui Ye, Lanfeng Xing, Fang Zhang, Yanjun Hu, Runjv Zhang, Ye Chun Ruan, Dan Zhang
Summary: Female fertility declines with age, and the metabolites in follicular fluids are closely related to oocyte quality. This study analyzed the metabolomic profile of follicular fluids from women of different ages and identified metabolites associated with oocyte quality and the outcomes of assisted reproductive technology (ART). These metabolites may serve as potential biomarkers for oocyte maturation and ART outcomes.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Plant Sciences
Trevor B. Romsdahl, Jean-Christophe Cocuron, Mackenzie J. Pearson, Ana Paula Alonso, Kent D. Chapman
Summary: This study introduces a stepwise targeted lipidomics approach to characterize polar and non-polar lipid classes. By using hydrophilic interaction chromatography and reverse phase chromatography, changes among different lipid molecular species were found, and potential metabolic explanations were discussed.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Multidisciplinary Sciences
Maria Victoria Ruiz-Perez, Lourdes Sainero-Alcolado, Ganna Oliynyk, Isabell Matuschek, Nicola Balboni, S. J. Kumari A. Ubhayasekera, Marteinn Thor Snaebjornsson, Kamil Makowski, Kristina Aaltonen, Daniel Bexell, Dolors Serra, Roland Nilsson, Jonas Bergquist, Almut Schulze, Marie Arsenian-Henriksson
Summary: Metabolic pathways, especially lipid metabolism, are rewired in tumors to support their growth and adaptation to stressful environments. In neuroblastoma, inhibition of fatty acid synthesis led to increased neural differentiation and reduced tumor burden independently of MYCN-status, showing promise as a pharmacological intervention strategy.
Article
Pharmacology & Pharmacy
Mohammad Al-Mandi Al-Karagholi, Jakob Moller Hansen, Dalia Abou-Kassem, Anna Koldbro Hansted, Kumari Ubhayasekera, Jonas Bergquist, Laszlo Vecsei, Inger Jansen-Olesen, Messoud Ashina
Summary: The kynurenine pathway is a potential drug discovery target in neuroscience, with L-kynurenine emerging as a prodrug targeting glutamate receptors. LKYN was found to be safe and well-tolerated in humans at various dose levels, with no significant vascular effects observed after infusion. Further exploration of LKYN's therapeutic potential in stroke and epilepsy in human studies is warranted.
PHARMACOLOGY RESEARCH & PERSPECTIVES
(2021)
Article
Veterinary Sciences
Bodil S. Holst, Sofia Carlin, Virginie Fouriez-Lablee, Sofia Hanas, Sofie Odling, Liss-Marie Langborg, S. J. Kumari A. Ubhayasekera, Jonas Bergquist, Jesper Ryden, Elin Holmroos, Kerstin Hansson
Summary: CPSE concentration is associated with future growth of the prostate in dogs, while concentrations of corticosterone and corticosteroids are positively associated with the change in relative prostatic size over time. Dogs with larger prostates at baseline are more likely to develop clinical signs of prostatic disease during follow-up.
BMC VETERINARY RESEARCH
(2021)
Article
Endocrinology & Metabolism
Johanna Christina Penell, Mark M. Kushnir, Lars Lind, Jonatan Bergquist, Jonas Bergquist, P. Monica Lind, Tord Naessen
Summary: This study examined sex-specific plasma concentrations of endogenous sex and adrenal steroids in elderly Swedish Caucasians, finding that estradiol, estrone, and testosterone levels were higher in 70-year-old men compared to women. Body mass index was positively associated with estradiol and estrone in women, while negatively associated with testosterone in men. Apparently healthy individuals showed differences in hormone concentrations compared to all individuals.
ENDOCRINE CONNECTIONS
(2021)
Article
Biochemistry & Molecular Biology
Henrik Hill, Andris Elksnis, Per Lundkvist, Kumari Ubhayasekera, Jonas Bergquist, Bryndis Birnir, Per-Ola Carlsson, Daniel Espes
Summary: Gamma-aminobutyric acid (GABA) is an important inhibitory neurotransmitter found in the central nervous system and immune cells. Research has shown that GABA has immune-modulatory and beta-cell stimulatory effects and could potentially be used to treat type 1 diabetes. However, a study measuring the levels of GABA in T1D patients found that the levels were unchanged compared to healthy controls, suggesting that GABA secretion from beta-cells has minimal impact on systemic levels. The study also found correlations between GABA levels and certain cytokines and GAD-autoantibodies, indicating potential alterations in local GABA levels within pancreatic islets.
Article
Oncology
Yingya Zhao, Yu-Tang Gao, Xianglan Zhang, Alan L. Rockwood, Mark M. Kushnir, Qiuyin Cai, Jie Wu, Jiajun Shi, Qing Lan, Nathaniel Rothman, Yu Shyr, Xiao-Ou Shu, Wei Zheng, Gong Yang
Summary: The study suggests that higher levels of circulating free testosterone and estimated aromatase activity may be associated with lower lung cancer risk in postmenopausal never-smoking women.
INTERNATIONAL JOURNAL OF CANCER
(2022)
Article
Pharmacology & Pharmacy
Amol O. Bajaj, Mark M. Kushnir, Erik Kish-Trier, Rachel N. Law, Lauren M. Zuromski, Alejandro R. Molinelli, Gwendolyn A. McMillin, Kamisha L. Johnson-Davis
Summary: We developed an LC-MS/MS method for monitoring the concentrations of 6-TG and 6-MMP and evaluated the stability of thiopurine metabolites. The method showed good agreement with other methods and revealed differences in metabolite concentrations among different TPMT groups and age groups.
FRONTIERS IN PHARMACOLOGY
(2022)
Review
Medical Laboratory Technology
Mark M. Kushnir, Heather A. Nelson, Kelly Doyle
Summary: This review discusses the techniques used in the separation and direct quantification of FH concentrations in biological samples using mass spectrometry. Measurement of FH presents challenges, but equilibrium dialysis, ultrafiltration, or size-exclusion separation coupled with liquid chromatography-tandem mass spectrometry provide sensitive and specific methods for measurement.
JOURNAL OF APPLIED LABORATORY MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Wei Hu, Yaorui Hu, Yao Pei, Rongrong Li, Fuyi Xu, Xiaodong Chi, Jia Mi, Jonas Bergquist, Lu Lu, Luping Zhang, Chunhua Yang
Summary: A study found that there were 46 differentially expressed proteins between cervical carcinoma (CC) and normal tissues, with Integrin beta 6, PPP1CB, TMPO, PTGES3, and DTX3L significantly upregulated in CC, while Desmin was significantly downregulated. Silencing DTX3L could suppress CC cell proliferation, migration, invasion, and tumorigenesis, and enhance cell apoptosis. Furthermore, DTX3L silencing inhibited the activity of the PI3K/AKT/mTOR signaling pathway. These findings suggest that DTX3L may regulate CC progression through the PI3K/AKT/mTOR signaling pathway and could serve as a potential biomarker and therapeutic target for CC.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemical Research Methods
Carmen Dunbar, Mark M. Kushnir, Yifei K. Yang
Summary: AFP serves as a serum biomarker for liver cirrhosis and hepatocellular carcinoma, with different glycosylation patterns distinguishing these conditions. Core fucosylated AFP-L3 is a marker for malignancy risk in HCC. A method combining immune enrichment, LC-HRMS, and protein analysis was developed to detect endogenous AFP proteoforms and quantify AFP-L3% in serum. The method showed reproducibility and agreement with a commercial assay, and identified AFP glycoforms without core fucosylation (AFPL1) and common AFP-L3 glycoforms (G2FS1 and G2FS2).
JOURNAL OF PROTEOME RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Sofia Persson, Kumari A. Ubhayasekera, Jonas Bergquist, Sahruh Turkmen, Inger Sundstroem Poromaa, Evangelia Elenis
Summary: Polycystic ovary syndrome (PCOS) is a common endocrine disorder among women, and hyperandrogenism can cause psychological morbidity and impaired quality of life. This study aimed to investigate whether hyperandrogenism persists into midlife and its impact on quality of life.
Correction
Biochemical Research Methods
Carmen Dunbar, Mark M. Kushnir, Yifei K. Yang
JOURNAL OF PROTEOME RESEARCH
(2023)
Article
Food Science & Technology
Julio Cesar Espana Amortegui, Heidi Pekar, Mark Dennis Chico Retrato, Malin Persson, Bengt Karlson, Jonas Bergquist, Aida Zuberovic-Muratovic
Summary: A LC-MS/MS method was developed for the simultaneous identification and quantification of cyanotoxins in edible bivalves. The method successfully detected and resolved two previously detected cyanotoxin congeners separately. The method showed limitations for certain cyanotoxins but overall was specific and robust for the parameters investigated. The method was applied to analyze mussel and oyster samples collected along the Swedish coast, and the results can contribute to future regulatory monitoring for cyanotoxins to ensure seafood safety.
Article
Infectious Diseases
Anja Naas, Peng Li, Clas Ahlm, Elisabeth Aurelius, Josef D. Jarhult, Silvia Schliamser, Marie Studahl, Wenzhong Xiao, Jonas Bergquist, Gabriel Westman
Summary: This study investigated the temporal changes of the CSF proteome in patients with herpes simplex encephalitis (HSE) and its association with factors such as anti-NMDAR serostatus, corticosteroid treatment, brain MRI, and neurocognitive performance. The results showed that the composition and activation pathways of CSF proteins vary at different stages of the disease. Additionally, it was found that several proteins were less abundant in patients with anti-NMDAR seropositivity compared to seronegativity.
INFECTIOUS DISEASES
(2023)
Article
Chemistry, Analytical
Mark Dennis Chico Retrato, Siyuan Qiu, Anna Lundquist, Aida Zuberovic Muratovic, Farshid Mashayekhy Rad, S. J. Kumari A. Ubhayasekera, Jonas Bergquist
Summary: This study describes a simple and robust method for the identification and quantification of 22 fatty acids in TPN components using gas chromatography-mass spectrometry. The method was evaluated according to the guidelines from FDA, EMA, and ICH, and demonstrated good methodological performance. The developed method has potential applications for quality assurance and control of TPN products.
ANALYTICAL METHODS
(2023)
Article
Biochemistry & Molecular Biology
Maruf Hasan, Henry Reyer, Michael Oster, Nares Trakooljul, Siriluck Ponsuksilli, Elizabeth Magowan, Dagmar -Christiane Fischer, Klaus Wimmers
Summary: UVB exposure can increase the supply of vitamin D in pig husbandry and does not affect the growth performance of the pigs. After exposure, there are changes in gene expression in the liver, with the pathways for vitamin D synthesis being preferentially initiated.
JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY
(2024)
Article
Biochemistry & Molecular Biology
Zhen Wang, Shu-ying Yi, Yuan-ying Zhang, Yu-di Wang, Han-lin Chen, Yi-jie Guo, Xin-ming Wei, Du-xiao Yang
Summary: Vitamin D can reverse S1P-induced cell death and alleviate EAE symptoms by regulating S1P levels and related signaling pathways.
JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY
(2024)
Correction
Biochemistry & Molecular Biology
Haixiao Chen, Xing Ji, Xinhua Hu, Lihua Chen, Haiyan Lv, Chengyun Xu, Dun Hong, Ximei Wu
JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY
(2024)