Article
Cell Biology
Yukiko Imai, Kenji Saito, Kazumasa Takemoto, Fabien Velilla, Toshihiro Kawasaki, Kei-ichiro Ishiguro, Noriyoshi Sakai
Summary: The mutation in the zebrafish gene encoding the transverse filament protein Sycp1 leads to incomplete pairing of homologs in zebrafish meiosis, while not affecting double-strand break formation near telomeres.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Catriona Munro, Hugo Cadis, Sophie Pagnotta, Evelyn Houliston, Jean-Rene Huynh
Summary: During meiosis, DNA recombination mediated by Spo11 enzyme triggers double-strand breaks (DSBs), which lead to the shuffling of genetic information between maternal and paternal chromosomes. In a study on the jellyfish Clytia hemisphaerica, they found that knocking down the Spo11 gene resulted in the failure of germ cells to assemble synaptonemal complexes and chiasmata, leading to the presence of unassociated homologous chromosome pairs and aneuploid but fertilizable eggs. This study provides significant evolutionary perspectives on meiosis regulation, as Clytia belongs to the sister clade of classical animal model species.
Review
Cell Biology
Corinne Grey, Bernard de Massy
Summary: The axial element plays a crucial role in establishing sister chromatid cohesion and meiotic recombination during prophase I of meiosis, contributing to the successful outcome of meiosis I.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Cell Biology
Elena Llano, Alberto M. Pendas
Summary: The synaptonemal complex (SC) forms during meiosis I and is involved in the synapses of homologous chromosomes, repair of double-strand breaks (DSBs), and formation of crossovers (COs). Defects in SC assembly or meiotic recombination machinery can cause meiotic arrest and infertility. Many proteins involved in these processes are meiosis-specific, but some also have roles in somatic DNA repair and are associated with cancer development.
Review
Biochemistry & Molecular Biology
Hideo Tsubouchi
Summary: Homologous recombination (HR) is crucial for meiosis and is induced during meiotic prophase. The Hop2-Mnd1 complex, originally identified in budding yeast, is conserved across species and plays an essential role in meiosis. Accumulating evidence suggests that Hop2-Mnd1 promotes RecA-like recombinases for homology search and strand exchange. This review summarizes studies on the mechanism of the Hop2-Mnd1 complex in promoting HR and beyond.
Article
Reproductive Biology
M. Bugno-Poniewierska, W. Witarski, M. Kuchta-Gladysz, A. Jakubczak, G. Jezewska-Witkowska
Summary: The study found that male interspecific hybrids of the red fox and the arctic fox exhibit meiotic division abnormalities, characterized by chromosomal pairing anomalies and disrupted meiotic checkpoint signaling, leading to a high percentage of apoptotic cells in the gonads and resulting in sterility.
Article
Cell Biology
Michael G. Pollard, Beth Rockmill, Ashwini Oke, Carol M. Anderson, Jennifer C. Fung
Summary: The synaptonemal complex (SC) is a structure formed during meiosis that stabilizes and supports essential processes such as pairing and recombination. This study measured the assembly and disassembly kinetics of the SC protein Zip1 in live cells of S. cerevisiae. It was found that SC assembly occurs with both monophasic and biphasic kinetics, and there are distinct disassembly events termed abortive disassembly. These findings provide new insights into the regulation of SC assembly and disassembly.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Yongliang Shang, Ju Huang, Weidong Li, Yanan Zhang, Xu Zhou, Qiqi Shao, Taicong Tan, Shen Yin, Liangran Zhang, Shunxin Wang
Summary: MEIOK21 plays important roles in regulating female meiosis and the number and quality of oocytes. Lack of MEIOK21 leads to a decrease in oocyte number, poor oocyte quality, and defects in meiotic recombination and crossover maturation.
Article
Multidisciplinary Sciences
Laia Capilla-Perez, Stephanie Durand, Aurelie Hurel, Qichao Lian, Aurelie Chambon, Christelle Taochy, Victor Solier, Mathilde Grelon, Raphael Mercier
Summary: The spatial organization of transverse filaments is essential for CO formation during meiosis, with the absence of ZYP1 leading to an unexpected increase in CO formation. The tripartite synaptonemal complex plays a key role in regulating the number and distribution of COs, affecting CO interference and heterochiasmy.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Oncology
Rosario Ortiz, Silvia Juarez Chavero, Olga M. Echeverria, Abrahan Hernandez-Hernandez
Summary: The study utilized an ultrastructural DNA staining technique on mouse testis to observe the organization of DNA in the SC. Bubble-like staining patterns were observed in certain regions, indicating that the specific organization of DNA is determined by chromosome synapsis, regardless of homology or partial SC formation.
EXPERIMENTAL CELL RESEARCH
(2021)
Article
Multidisciplinary Sciences
Martin G. France, Janina Enderle, Sarah Roehrig, Holger Puchta, F. Chris H. Franklin, James D. Higgins
Summary: The study on the function of the synaptonemal complex in Arabidopsis indicates that it limits the formation of Class I crossover interference and disrupts the progressive alignment of chromosome axes in plants.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Review
Genetics & Heredity
Sarah N. Ur, Kevin D. Corbett
Summary: Meiotic cell division is a specialized process preparing for sexual reproduction, involving homologous chromosomes identifying each other and forming physical links through DNA recombination. The SMC family cohesin complexes and meiotic chromosome axis play crucial roles in organizing chromosomes and promoting recombination. The synaptonemal complex (SC) ensures high fidelity in meiotic recombination by assembling between homologs and providing feedback.
ANNUAL REVIEW OF GENETICS, VOL 55
(2021)
Article
Cell Biology
Roberto de la Fuente, Florencia Pratto, Abrahan Hernandez-Hernandez, Marcia Manterola, Pablo Lopez-Jimenez, Rocio Gomez, Alberto Viera, Maria Teresa Parra, Anna Kouznetsova, R. Daniel Camerini-Otero, Jesus Page
Summary: This study investigates the impact of disruptions in recombination and synapsis on the dynamics of epigenetic markers and transcriptional regulation during meiosis in knockout mice. Results show that impairment of recombination and synapsis alters the epigenetic marks and transcriptional regulation, suggesting a crucial role in the impairment of meiosis progression in mammals.
Review
Cell Biology
Funda M. Kar, Andreas Hochwagen
Summary: Germ cells rely on a complex network of surveillance mechanisms to ensure the production of euploid gametes during meiosis. Dynamic protein phosphorylation, controlled by kinases and phosphatases, plays a crucial role in regulating chromosomal and cellular behavior throughout meiotic prophase. This review discusses common principles and detailed examples of how phosphorylation events are utilized to ensure faithful passage of chromosomes from one generation to the next.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Nathalie Vrielynck, Katja Schneider, Marion Rodriguez, Jason Sims, Aurelie Chambon, Aurelie Hurel, Arnaud De Muyt, Arnaud Ronceret, Ondrej Krsicka, Christine Mezard, Peter Schloegelhofer, Mathilde Grelon
Summary: Research suggests that in the initiation of meiotic recombination, MTOPVIB and PRD1 provide links between different proteins, PRD3/AtMER2 establishes a direct connection between DSB formation and recombination mechanisms, and DFO controls the formation of MTOPVIB foci.
NUCLEIC ACIDS RESEARCH
(2021)
Article
Optics
Tatsuki Tahara, Takako Koujin, Atsushi Matsuda, Ayumi Ishii, Tomoyoshi Ito, Yasuyuki Ichihashi, Ryutaro Oi
Summary: Color fluorescence imaging of self-luminous objects and stained biological samples is achieved using incoherent digital holographic technique with multiplexing of multiple wavelengths, self-interference, and computational coherent superposition. This method allows for simultaneous color three-dimensional sensing of multiple self-luminous objects without mechanical scanning, and demonstrates improved point spread function in color fluorescence imaging through experimental verification of a Fresnel incoherent correlation holography system.
Article
Biology
Brandon R. Lowe, Rajesh K. Yadav, Ryan A. Henry, Patrick Schreiner, Atsushi Matsuda, Alfonso G. Fernandez, David Finkelstein, Margaret Campbell, Satish Kallappagoudar, Carolyn M. Jablonowski, Andrew J. Andrews, Yasushi Hiraoka, Janet F. Partridge
Summary: The study highlights the varied phenotypic effects and impact on DNA repair caused by mutations in different types of H3 histones, indicating the complexity associated with substitutions at a single residue in H3. Analysis using genetically tractable systems demonstrates the utility of such approaches in studying these mutations.
Article
Biochemistry & Molecular Biology
Da-Qiao Ding, Atsushi Matsuda, Kasumi Okamasa, Yasushi Hiraoka
Summary: Upon entering meiosis, the structure of chromosomes changes significantly, forming multilayer proteinaceous structures such as SC and LinEs. LinEs form along the chromosome axis and elongate during meiosis, with their fine structure and dynamics requiring further investigation.
Article
Cell Biology
Megumi Tsuchiya, Hidesato Ogawa, Kento Watanabe, Takako Koujin, Chie Mori, Kazuto Nunomura, Bangzhong Lin, Akiyoshi Tani, Yasushi Hiraoka, Tokuko Haraguchi
Summary: Inhibiting p62/SQSTM1 receptor can increase DNA transfection efficiency, and microtubule inhibitors have positive effects in the presence of p62.
Article
Biochemistry & Molecular Biology
Reito Watanabe, Yasuhiro Hirano, Masatoshi Hara, Yasushi Hiraoka, Tatsuo Fukagawa
Summary: The kinetochore, essential for chromosome segregation during mitosis, is assembled through dynamic processes involving numerous kinetochore proteins. The study used fluorescence recovery after photobleaching (FRAP) analysis to reveal the dynamics of kinetochore assembly. Results showed that CENP-C is mobile during interphase but immobile during mitosis, while CENP-T and CENP-H are immobile during both interphase and mitosis. The mobility of Dsn1, a component of the Mis12 complex, depended on CENP-C mobility during mitosis.
CHROMOSOME RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Yoko Hayashi-Takanaka, Yuichiro Hayashi, Yasuhiro Hirano, Atsuko Miyawaki-Kuwakado, Yasuyuki Ohkawa, Chikashi Obuse, Hiroshi Kimura, Tokuko Haraguchi, Yasushi Hiraoka
Summary: During the G1 phase of the cell cycle, the MCM hexamer transitions from a single hexamer to a double hexamer, influencing the progression of the cell cycle. The methylation levels of histone H4K20 are correlated with the formation of the double MCM hexamer, leading to cell cycle arrest.
NUCLEIC ACIDS RESEARCH
(2021)
Review
Genetics & Heredity
Takeshi Sakuno, Yasushi Hiraoka
Summary: The key difference between meiosis and mitosis is that during meiosis, pairing and segregation of homologous chromosomes occur, and there are significant changes in the morphology of sister chromatids. The meiosis-specific cohesin complex, especially the Rec8 subunit, plays a crucial role in regulating the pairing and recombination of homologous chromosomes in meiosis.
Article
Optics
Tatsuki Tahara, Yuichi Kozawa, Atsushi Matsuda, Ryutaro Oi
Summary: The proposed technique utilizes LED for single-path phase-shifting digital holography, with a self-reference digital holography method to generate a reference wave for recording the quantitative phase information of the object wave, resulting in quantitative phase images of the objects. The validity of the technique is experimentally demonstrated by constructing an optical system with a wide-field optical microscope.
Article
Biochemistry & Molecular Biology
Takeshi Sakuno, Sanki Tashiro, Hideki Tanizawa, Osamu Iwasaki, Da-Qiao Ding, Tokuko Haraguchi, Ken-ichi Noma, Yasushi Hiraoka
Summary: The Rec8-dependent axis-loop structure formed during meiotic prophase is essential for LinE assembly and facilitates meiotic recombination of homologous chromosomes, independent of its role in sister chromatid cohesion.
NUCLEIC ACIDS RESEARCH
(2022)
Article
Cell Biology
Haruhiko Asakawa, Yasuhiro Hirano, Tomoko Shindo, Tokuko Haraguchi, Yasushi Hiraoka
Summary: This study reveals the regulated interaction between Ish1 and Les1, two nuclear envelope proteins in fission yeast. They are localized within the nuclear envelope lumen and show distinguishable localization during the end of mitosis.
Article
Cell Biology
Megumi Tsuchiya, Weixia Kong, Yasushi Hiraoka, Tokuko Haraguchi, Hidesato Ogawa
Summary: DNA transfection efficiency can be improved by inhibiting the phosphorylation process of p62 by TBK1, which reduces the intracellular degradation of transfected DNA.
Article
Biochemistry & Molecular Biology
Haruka Hiraoka, Jiewen Wang, Tadashi Nakano, Yasuhiro Hirano, Shinichi Yamazaki, Yasushi Hiraoka, Tokuko Haraguchi
Summary: Cell migration is crucial in the development of multicellular organisms, and Dictyostelium discoideum is a useful model organism for studying this process. This study reveals that cells with high ATP levels can move against the bulk flow, suggesting a mechanism by which ATP levels determine cell fate during differentiation.
Article
Cell Biology
Hui-Ju Yang, Haruhiko Asakawa, Fu-An Li, Tokuko Haraguchi, Hsiu-Ming Shih, Yasushi Hiraoka
Summary: The nuclear pore complex (NPC) acts as a permeable barrier between the nucleus and cytoplasm. Deficiency in nucleoporin Nup132 led to transient leakage of nuclear proteins during meiosis I in the fission yeast Schizosaccharomyces pombe. The leakage was accompanied by the release of SUMO-specific protease Ulp1 from the NPC. Retention of Ulp1 at the nuclear pore prevented protein leakage and restored normal meiosis.
Article
Cell Biology
Yasuhiro Hirano, Yusuke Ohno, Yoshino Kubota, Tatsuo Fukagawa, Akio Kihara, Tokuko Haraguchi, Yasushi Hiraoka
Summary: Recent studies have revealed that enzymes catalyzing lipid synthesis play a role in maintaining the integrity of the nuclear envelope (NE), but the mechanism involved remains unclear. In this study, the ceramide synthase homolog, Tlc4, was found to suppress NE defects in cells lacking the NE proteins Lem2 and Bqt4. Tlc4, which possesses a conserved TRAM/LAG1/CLN8 domain, functions through its non-catalytic activity and localizes at the NE, endoplasmic reticulum, and cisand medial-Golgi cisternae. The Golgi localization of Tlc4 is closely linked to its activity of suppressing NE defects in the double-deletion mutant of Lem2 and Bqt4.
JOURNAL OF CELL SCIENCE
(2023)
Review
Imaging Science & Photographic Technology
Joseph Rosen, Simon Alford, Vijayakumar Anand, Jonathan Art, Petr Bouchal, Zdenek Bouchal, Munkh-Uchral Erdenebat, Lingling Huang, Ayumi Ishii, Saulius Juodkazis, Nam Kim, Peter Kner, Takako Koujin, Yuichi Kozawa, Dong Liang, Jun Liu, Christopher Mann, Abhijit Marar, Atsushi Matsuda, Teruyoshi Nobukawa, Takanori Nomura, Ryutaro Oi, Mariana Potcoava, Tatsuki Tahara, Bang Le Thanh, Hongqiang Zhou
Summary: Fresnel incoherent correlation holography (FINCH) is a significant advancement in incoherent holography, with discussions on its development, applications, current technology, challenges, and future prospects presented by researchers from various fields contributing to FINCH research.
JOURNAL OF IMAGING
(2021)