Article
Pharmacology & Pharmacy
Ganlan Zhang, Hui Liu, Ting Xue, Xiangming Kong, Dongmei Tian, Libo Luo, Yanhua Yang, Keqing Xu, Youheng Wei, Ziheng Zhuang
Summary: Aging is a process of degenerative changes that cause diseases and disabilities. Research aims to find drugs that can extend lifespan and treat age-related diseases. Ribavirin has been discovered as a potential drug for aging intervention by inhibiting TOR signaling and activating AMPK. Its anti-aging effect is mediated via AMPK-TOR signaling.
EUROPEAN JOURNAL OF PHARMACOLOGY
(2023)
Article
Immunology
Dan He, Qiangdongzi Mao, Jialin Jia, Zhiyu Wang, Yu Liu, Tingting Liu, Bangwei Luo, Zhiren Zhang
Summary: This study reveals that the pentose phosphate pathway (PPP) regulates the clearance of tolerogenic apoptotic cells (ACs) and immune tolerance. ACs decrease the levels of PPP-related genes and metabolites in macrophages. The PPP agonist AG1 increases PPP activity but greatly reduces macrophage phagocytosis of ACs and enhances the inflammatory response during efferocytosis. MiR-323-5p regulates the expression of PPP-related genes and its levels increase during efferocytosis.
FRONTIERS IN IMMUNOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Khairunnisa Mohd Kamal, Mohd Hafidz Mahamad Maifiah, Yan Zhu, Nusaibah Abdul Rahim, Yumi Zuhanis Has-Yun Hashim, Muhamad Shirwan Abdullah Sani
Summary: The pentose phosphate pathway (PPP) is important in metabolism for the generation of NADPH, nucleotide synthesis, and carbon homeostasis. Isotope tracer studies have improved the analysis and understanding of PPP, but face limitations. The Group Specific Internal Standard Technology (GSIST) is an in vitro method that allows for measuring absolute levels of PPP metabolites.
Article
Biology
Brooke Zanco, Christen K. Mirth, Carla M. Sgro, Matthew D. W. Piper
Summary: Research on fruit flies shows that the influence of protein and carbohydrates on lifespan is indirect, affecting the distribution of dietary sterols. Supplementing with cholesterol can rescue the shortened lifespan caused by high protein: carbohydrate diets. This highlights the complex and indirect effects of nutrient-dependent trade-offs on life histories.
Article
Biochemistry & Molecular Biology
Yu-Chin Nieh, Yu-Ting Chou, Chao-Yung Wang, Shi-Xian Lin, Shih-Ci Ciou, Chiou-Hwa Yuh, Horng-Dar Wang
Summary: RPIA plays a crucial role in lung cancer by regulating cellular proliferation and activating autophagy, apoptosis, and cellular senescence. Suppression of RPIA leads to increased reactive oxygen species (ROS) levels, which in turn induce autophagy, apoptosis, and cellular senescence in lung cancer cells. This study sheds new light on the potential of RPIA suppression as a therapeutic strategy for lung cancer.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Paloma Garcia-Casas, Pilar Alvarez-Illera, Rosalba Fonteriz, Mayte Montero, Javier Alvarez
Summary: Recent reports have shown that submaximal inhibition of the Sarco Endoplasmic Reticulum Ca2+ ATPase (SERCA) increases the lifespan of C. elegans worms. This increase in survival is not related to the insulin signaling pathway or sirtuin activity, but requires functional mitochondria and the AMP kinase and TOR pathways. Moreover, the effect may be due to a reduced ER-mitochondria Ca2+ transfer, promoting survival through AMPK activation and mTOR inhibition.
MECHANISMS OF AGEING AND DEVELOPMENT
(2021)
Article
Biochemistry & Molecular Biology
Qian Zheng, Rong Wang, Xin Ju, Xujing Gu, Xinqi Xu, Zhi Chen, Liangzhi Li
Summary: In this study, ribose-5-phosphate isomerase A (OsRpiA) from Ochrobactrum sp. CSL1 was engineered to obtain a triple-mutant strain A10T/T32S/G101N with 160% activity, and the enzymatic properties of the mutant were systematically investigated. This study provides a solid basis for rare sugar preparation and demonstrates the complex functions of crucial residues in RpiAs.
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Ziyu Han, Na Li, Hong Xu, Zheng Xu
Summary: In this study, a C-terminal a-helix from L-arabinose isomerase (L-AI) family was engineered and connected to Lactobacillus fermentum L-AI (LFAI) protein, enhancing its catalytic activity and stability for L-arabinose and galactose. A thermostable mannose-6-phosphate isomerase from Bacillus subtilis (BsMPI-2) was also immobilized and showed high efficiency in catalyzing L-ribu-lose. The dual immobilized enzymes system co-produced L-ribose and D-tagatose with high yields, showing excellent industrial potential.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Microbiology
Hui Lin, Pengfei Li, Lu Ma, Shufang Lai, Shujing Sun, Kaihui Hu, Liaoyuan Zhang
Summary: This study investigated the effects of central carbon metabolism (CCM) on the mycelial growth performance and fruiting body formation of Hypsizygus marmoreus. The study found that imbalanced distribution of intracellular carbon metabolic levels in the CCM might be one of the key factors resulting in a slow mycelial growth rate and a long harvest period. By increasing the expression levels of glucose-6-phosphate dehydrogenase (G6PDH), the study successfully improved the mycelial growth and fruiting body yield of H. marmoreus.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Multidisciplinary Sciences
Jason Driver Tucker, Ravi Doddapaneni, Pei Juan Lu, Qi Long Lu
Summary: Breast cancer is a heterogeneous disease with various factors affecting prognosis. Metabolic alterations have recently gained attention in breast cancer research, and the effects of different sugars on breast cancer cells were investigated using metabolomics and gene expression profiling, revealing significant changes in metabolic pathways.
Article
Food Science & Technology
Junhyo Cho, Yeonhwa Park
Summary: Coffee's kahweol compound has been found to extend the lifespan of C. elegans, independent of reduced food intake. It acts through the insulin/insulin-like growth factor-1 signaling pathway and may involve the AMPK pathway.
CURRENT RESEARCH IN FOOD SCIENCE
(2023)
Review
Biotechnology & Applied Microbiology
Hengtao Tang, Xin Ju, Jing Zhao, Liangzhi Li
Summary: This review highlights the pivotal roles of RpiB in carbon metabolism, discusses its sequence identity and structural similarity, and provides a solid foundation for enzyme engineering. The differences between RpiB and other isomerases are introduced, and the potential of RpiB in rare sugar production is emphasized.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Chemistry, Physical
Hengtao Tang, Zheng Zhou, Zhi Chen, Xin Ju, Liangzhi Li
Summary: Monosaccharides from biomass can be used as cost-effective raw materials to produce valuable rare sugars. Enzymes play a crucial role in biomass biorefinery processes by developing novel synthetic routes and improving environmental friendliness. This study proposes a biorefinery process to prepare rare sugars of D-xylulose and D-ribulose from D-xylose, and demonstrates the promising role of monosaccharide isomerase platforms in biomass refining by constructing an enzyme cascade.
MOLECULAR CATALYSIS
(2022)
Article
Biotechnology & Applied Microbiology
Ling Zhang, Ming-Yue Nie, Feng Liu, Jun Chen, Liu-Jing Wei, Qiang Hua
Summary: In this study, a multiple gene integration strategy was employed to enhance erythritol production in Y. lipolytica. Overexpression of key genes such as TKL1 and TAL1, as well as disruption of the EYD1 gene, led to increased erythritol production. Further enhancement was observed by overexpressing the RKI1 and AMPD genes, indicating their importance in erythritol production in Y. lipolytica.
BIOTECHNOLOGY LETTERS
(2021)
Review
Biochemistry & Molecular Biology
Dina Alramadhani, Anfal S. Aljahdali, Osheiza Abdulmalik, B. Daniel Pierce, Martin K. Safo
Summary: Sickle cell disease is a genetic disorder that affects millions of individuals worldwide and is associated with chronic anemia, hemolysis, and vasculopathy. The understanding of altered metabolism in sickle red blood cells highlights potential targets for the development of novel therapeutics for the disease.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Oncology
Zong-Lin Sie, Ruei-Yang Li, Bonifasius Putera Sampurna, Po-Jui Hsu, Shu-Chen Liu, Horng-Dar Wang, Chou-Long Huang, Chiou-Hwa Yuh
Article
Oncology
Cam-Thu Ha, Chih-Yuan Cheng, Ming-Yi Zheng, Tang-Hui Hsu, Chia-Cheng Miao, Chang-Jung Lee, Horng-Dar Wang, Shien-Tung Pan, Yu-Ting Chou
Summary: The ID4-BDNF signaling pathway plays a crucial role in colorectal cancer, affecting tumor growth, migration, and chemosensitivity.
Article
Cell Biology
Tai-Ting Lee, Po-Lin Chen, Matthew P. Su, Jian-Chiuan Li, Yi-Wen Chang, Rei-Wen Liu, Hsueh-Fen Juan, Jinn-Moon Yang, Shih-Peng Chan, Yu-Chen Tsai, Sophia von Stockum, Elena Ziviani, Azusa Kamikouchi, Horng-Dar Wang, Chun-Hong Chen
Summary: By using the Drosophila melanogaster model, researchers discovered that the mitochondrial outer membrane protein Fis1 plays a crucial role in muscle dysfunction and several age-related conditions, with mutations leading to damaged mitochondria, increased oxidative stress, and decreased muscle function. Mutations in Fis1 also affect mitochondrial proteostasis in fly muscle, reducing flight capabilities and lifespan, indicating the significance of Fis1 in fly mitochondrial dynamics.
Article
Medicine, General & Internal
I-Jung Tsai, Wen-Chi Shen, Chia-Ling Lee, Horng-Dar Wang, Ching-Yu Lin
Summary: This study utilized clinical laboratory data and machine learning methods to build predictive models of bladder cancer. The lightGBM model showed promising accuracy and sensitivity in discriminating bladder cancer.
Article
Oncology
Chi-Feng Tseng, Li-Tzong Chen, Horng-Dar Wang, Yi-Hong Liu, Shine-Gwo Shiah
Summary: The HOXB-AS3/EZH2 complex plays a significant role in causing sorafenib resistance and enhancing cancer stemness by epigenetically suppressing Dicer expression in liver cancer. These findings provide potential therapeutic strategies for diagnosing and treating liver cancer patients.
Article
Biochemistry & Molecular Biology
Zhi-Han Lin, Shun-Ya Chang, Wen-Chi Shen, Yen-Hung Lin, Chiu-Lun Shen, Sin-Bo Liao, Yu-Chun Liu, Chang-Shi Chen, Tsui-Ting Ching, Horng-Dar Wang
Summary: The expression of idha-1 gene modulates lifespan and oxidative stress tolerance in C. elegans. Overexpression of idha-1 extends lifespan, increases NADPH/NADP(+) ratio, and elevates oxidative stress tolerance. Knockdown of idha-1 has the opposite effects. idha-1 may play a role in dietary restriction-mediated longevity and its regulation may involve the TOR signaling pathway.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Materials Science, Biomaterials
He-Yun Hsiao, Po-Jui Hsu, Bonifasius Putera Sampurna, Yu-Ju Lin, Kuan-Hao Lin, Yu-Ning Zhao, Horng-Dar Wang, Chiou-Hwa Yuh
Summary: The traditional Chinese medicine Carassius auratus complex formula (CACF) is known for its antidiabetic effects. This study explores the comprehensive effects and mechanisms of CACF on hepatocellular carcinoma (HCC), a major cause of cancer-related deaths. The results show that CACF reduces cell viability in HCC while benefiting normal hepatocytes. It also inhibits HCC growth in a zebrafish model, decreases lipid accumulation, represses inflammation and cell proliferation markers, and inhibits the expression of cell proliferation and beta-catenin downstream targets. CACF exerts multiple functions, including reduction of inflammation, inhibition of lipid transporter and PPAR signaling pathway, and even reduction of coronavirus infection and pathogenesis in a zebrafish model.
Article
Biochemistry & Molecular Biology
Chia-Ying Hou, Chung-Yung Ma, Yu-Ju Lin, Chou-Long Huang, Horng-Dar Wang, Chiou-Hwa Yuh
Summary: Lysine-deficient protein kinase-1 (WNK1) is found to be upregulated in both embryonic and tumor-induced angiogenesis. The downstream effectors of WNK1 are identified as oxidative stress responsive 1b (osr1b) in endothelial cells, and protein phosphatase 2A (pp2a) subunit ppp2r1bb is downregulated. WNK1 plays a critical role in promoting hepatoma cell migration in both endothelial cells and hepatoma cells, with OSR1 being downstream of WNK1 in endothelial cells and PPP2R1A being a downstream effector in hepatoma. The combination therapy with WNK1 inhibitor and OSR1 inhibitor, along with oligo-fucoidan, shows significant anti-cancer efficacy in advanced hepatocellular carcinoma.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Yu-Chin Nieh, Yu-Ting Chou, Chao-Yung Wang, Shi-Xian Lin, Shih-Ci Ciou, Chiou-Hwa Yuh, Horng-Dar Wang
Summary: RPIA plays a crucial role in lung cancer by regulating cellular proliferation and activating autophagy, apoptosis, and cellular senescence. Suppression of RPIA leads to increased reactive oxygen species (ROS) levels, which in turn induce autophagy, apoptosis, and cellular senescence in lung cancer cells. This study sheds new light on the potential of RPIA suppression as a therapeutic strategy for lung cancer.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Po-Jui Hsu, Horng-Dar Wang, Yung-Che Tseng, Shao-Wei Pan, Bonifasius Putera Sampurna, Yuh-Jyh Jong, Chiou-Hwa Yuh
Summary: This study identified a novel de novo mutation in Tropomyosin 3 (TPM3) associated with congenital myopathy (CM). The mutant gene TPM3(E151G) showed distinct pathogenicity compared to TPM3(E151A) in transgenic fish, leading to impaired muscle function. Treatment with l-carnitine improved muscle endurance by restoring mitochondrial function and gene expression profiles, shedding light on potential therapeutic strategies for TPM3-related CM.
JOURNAL OF BIOMEDICAL SCIENCE
(2021)