Article
Polymer Science
Gerardo Asensio, Lorena Benito-Garzon, Rosa Ana Ramirez-Jimenez, Yasmina Guadilla, Julian Gonzalez-Rubio, Cristina Abradelo, Juan Parra, Maria Rocio Martin-Lopez, Maria Rosa Aguilar, Blanca Vazquez-Lasa, Luis Rojo
Summary: Regenerative therapies based on tissue engineering have great potential for the treatment of osteoarthritis and rheumatoid arthritis. However, the regeneration of full-thickness articular osteochondral defects that replicate the complexity of native cartilage and osteochondral interface is still challenging. In this study, we present a biomimetic scaffold that mimics the spatial organization and composition of cartilage and osteochondral interface. The scaffold shows desirable physical and chemical characteristics, sustained release of bioactive compounds, and promotes guided cell colonization and proliferation. In vivo experiments demonstrate the ability of the biomimetic scaffold to promote the regeneration of cartilage-like tissue and neoformation of osteochondral tissue.
Article
Engineering, Biomedical
Xiao Sheng, Chen Li, Zhonghan Wang, Yu Xu, Yang Sun, Weimin Zhang, He Liu, Jincheng Wang
Summary: This review provides a comprehensive overview of strontium-containing biomaterials and their current clinical use. Different types of biomaterials, including calcium phosphate, bioactive glass, and polymers, are discussed, and future outlook on the fabrication of the next-generation multifunctional and smart biomaterials is provided.
MATERIALS TODAY BIO
(2023)
Review
Polymer Science
Hee Sook Hwang, Chung-Sung Lee
Summary: Hydrogel-based bone tissue engineering using hyaluronic acid (HA) has gained popularity as a potential strategy for treating bone abnormalities and fractures. HA-based hydrogels have the ability to mimic the natural extracellular matrix of bone tissue and provide a suitable microenvironment for cell support and tissue regeneration. The physical and chemical properties of HA can be modified to improve its mechanical strength, biocompatibility, and osteogenic potential, making it a promising material for bone tissue engineering.
Article
Engineering, Biomedical
Joyce Rodrigues de Souza, Elisa Camargo Kukulka, Juliani Caroline Ribeiro Araujo, Tiago Moreira Bastos Campos, Renata Falchete do Prado, Luana Marotta Reis de Vasconcellos, Gilmar Patrocinio Thin, Alexandre Luiz Souto Borges
Summary: The aim of this study was to produce and test polymeric scaffolds composed of polylactic acid (PLA) fibers associated with a 58S bioglass doped with therapeutic ions for use in tissue engineering. Scaffolds were produced through electrospinning process and characterized chemical and morphologically. In vitro tests showed that all materials had cell viability (>70%), total protein production, and alkaline phosphatase activity. The results suggest that the development of polylactic acid scaffolds associated with 58S bioglass doped with therapeutic ions is feasible for bone tissue engineering.
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS
(2023)
Article
Engineering, Biomedical
Cam-Hoa Mac, Hao-Yu Chan, Yi-Hsuan Lin, Amit Kumar Sharma, Hsiang-Lin Song, Yi-Sheng Chan, Kun-Ju Lin, Yu-Jung Lin, Hsing-Wen Sung
Summary: This study proposes a biomaterial-based scaffold consisting of radially aligned mineralized collagen fibers and nanosilicon. The scaffold guides the directional infiltration and migration of reparative cells and releases hydrogen gas and silicic acid, promoting bone regeneration.
Article
Materials Science, Multidisciplinary
Zhuyun Cai, Heng Jiang, Tao Lin, Ce Wang, Jun Ma, Rui Gao, Yingying Jiang, Xuhui Zhou
Summary: Microspheres and microsphere-based scaffolds have shown great potential in translational medicine. This review summarizes the various fabrication methods for microspheres, discusses the limitations of existing techniques, and describes the appropriate materials and surface-modification strategies to enhance the biological properties of microspheres. The key application of microsphere-functionalized scaffolds for bone tissue engineering is also discussed.
MATERIALS TODAY ADVANCES
(2022)
Article
Engineering, Biomedical
Zhuo Li, Haixing Wang, Kunyu Zhang, Boguang Yang, Xian Xie, Zhengmeng Yang, Lingchi Kong, Peng Shi, Yuan Zhang, Yi-Ping Ho, Zhi-Yong Zhang, Gang Li, Liming Bian
Summary: The research assembled a biomimetic hyaluronic acid nanocomposite hydrogel with bisphosphonates (BPs), and demonstrated its expedited release of BPs in an acidic environment. The in vitro studies showed that the hydrogel inhibits mature osteoclastic differentiation, while promoting the initial differentiation of primary macrophages to preosteoclasts. In vivo evaluation confirmed the hydrogel's ability to enhance in situ bone regeneration.
BIOACTIVE MATERIALS
(2022)
Article
Biochemistry & Molecular Biology
Bruno Zwingenberger, Corina Vater, Roland L. Bell, Julia Bolte, Elisabeth Mehnert, Ronny Bruenler, Dilbar Aibibu, Stefan Zwingenberger
Summary: The study found that CHS scaffolds exhibited higher osteogenic properties in vitro, but resulted in lower bone-healing outcomes compared to CBA and MCM in vivo. Future improvements on CHS scaffolds are needed for better mechanical stability and osteoconductivity.
Review
Engineering, Biomedical
Guanyin Zhu, Tianxu Zhang, Miao Chen, Ke Yao, Xinqi Huang, Bo Zhang, Yazhen Li, Jun Liu, Yunbing Wang, Zhihe Zhao
Summary: Bone-tissue defects are a global issue, with autologous and allogeneic bone grafting failing to achieve ideal therapeutic effects. This has led researchers to explore novel bone-regeneration methods, with the development of BTE scaffolds at the forefront. By delving into bone physiology and healing mechanisms, biomimicking and bioinspired BTE scaffolds are making progress.
BIOACTIVE MATERIALS
(2021)
Article
Materials Science, Multidisciplinary
Milena S. Malherbi, Luciano C. Dias, Mariana S. Z. Lima, Larissa G. Ribeiro, Valdirlei F. Freitas, Taiana G. M. Bonadio, Ludmilla M. Silva, Gilvan B. Souza, Eduardo A. Volnistem, Jaciele M. Rosso, Daniel M. Silva, Luiz F. Cotica, Wilson R. Weinand, Ivair A. Santos
Summary: This study synthesized hydroxyapatite/beta-tricalcium phosphate/polyvinylidene fluoride biocomposites and used electrical stimuli to improve and accelerate bone regeneration in vitro. The results showed that the growth rate of hydroxyapatite in the biocomposite significantly increased under specific electric field conditions.
JOURNAL OF MATERIALS RESEARCH AND TECHNOLOGY-JMR&T
(2022)
Review
Engineering, Biomedical
Elia Bosch-Rue, Leire Diez-Tercero, Jenifer Olmos Buitrago, Emilio Castro, Roman A. Perez
Summary: Bone has the ability to self-regenerate, but extensive damage can hinder the natural regeneration process. Bone tissue engineering aims to mimic the extracellular matrix and promote the physiological regeneration process by stimulating osteogenesis and angiogenesis.
ACTA BIOMATERIALIA
(2023)
Review
Biotechnology & Applied Microbiology
V. K. Anupama Devi, Anjaneyulu Udduttula, Amit Kumar Jaiswal
Summary: Biomedical uses for natural polysaccharides of marine origin, including fucoidan derived from brown algae, are gaining popularity. Despite being less researched compared to other biomaterials, fucoidan is more accessible and cost-effective. This review provides an overview of its history, sources, and characteristics, with a focus on its application in bone tissue engineering. The global research status of fucoidan, especially for bone tissue engineering, is examined, making this study a comprehensive manual for understanding its research status.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Review
Nanoscience & Nanotechnology
Mingran Zhang, Jiaxue Liu, Tongtong Zhu, Hanxiang Le, Xukai Wang, Jinshan Guo, Guangyao Liu, Jianxun Ding
Summary: Bone adhesives are expected to have significant advantages in treating bone injuries compared to traditional internal fixation devices. Different research directions of bone adhesives, such as liquid scaffold and liquid plate, are summarized. Recent advances in mechanical strength, osseointegration, osteoconductivity, and osteoinductivity of bone adhesives are discussed.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Review
Cell & Tissue Engineering
Hongqi Zhao, Chaoxu Liu, Yang Liu, Qing Ding, Tianqi Wang, Hao Li, Hua Wu, Tian Ma
Summary: Bone tissue engineering (BTE) is a promising approach for bone defects by providing mechanical support for bone regeneration. The use of electromagnetic fields (EMFs) as a non-invasive therapy has shown positive effects on cell adhesion, mesenchymal stem cells, angiogenesis, and macrophage polarization in BTE. The combination of EMFs and diverse biomaterials has achieved encouraging outcomes, indicating a promising future for effectively treating bone defects.
STEM CELL RESEARCH & THERAPY
(2023)
Article
Engineering, Biomedical
Zhiyuan Zou, Le Wang, Zhifei Zhou, Qing Sun, Delong Liu, Yan Chen, Hao Hu, Yu Cai, Sixiong Lin, Zhengran Yu, Bizhi Tan, Wei Guo, Zemin Ling, Xuenong Zou
Summary: The use of hydrogels containing PTH and nHAP can promote cranial bone regeneration by enhancing the expression of osteogenic-related proteins and facilitating osteogenic differentiation of BMSCs through the Notch signaling pathway. Implantation of the hydrogel into a rat cranial defect model showed efficient bone regeneration compared to using the hydrogel alone or nHAP alone, indicating a promising therapeutic effect of combining nHAP and PTH for bone regeneration.
BIOACTIVE MATERIALS
(2021)
Article
Nanoscience & Nanotechnology
Miryam Criado-Gonzalez, Eva Espinosa-Cano, Luis Rojo, Fouzia Boulmedais, Maria Rosa Aguilar, Rebeca Hernandez
Summary: Supramolecular peptide-based hydrogels with excellent anti-inflammatory properties were developed in this study. The formation of the injectable hybrid hydrogel was triggered by electrostatic interactions between a phosphorylated tripeptide and cationic polymer nanoparticles. The hydrogel exhibited a fibrillary structure and shear thinning behavior, and demonstrated a significant reduction in nitric oxide levels, confirming its potential for the treatment of localized inflammatory pathologies.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Polymer Science
Gerardo Asensio, Lorena Benito-Garzon, Rosa Ana Ramirez-Jimenez, Yasmina Guadilla, Julian Gonzalez-Rubio, Cristina Abradelo, Juan Parra, Maria Rocio Martin-Lopez, Maria Rosa Aguilar, Blanca Vazquez-Lasa, Luis Rojo
Summary: Regenerative therapies based on tissue engineering have great potential for the treatment of osteoarthritis and rheumatoid arthritis. However, the regeneration of full-thickness articular osteochondral defects that replicate the complexity of native cartilage and osteochondral interface is still challenging. In this study, we present a biomimetic scaffold that mimics the spatial organization and composition of cartilage and osteochondral interface. The scaffold shows desirable physical and chemical characteristics, sustained release of bioactive compounds, and promotes guided cell colonization and proliferation. In vivo experiments demonstrate the ability of the biomimetic scaffold to promote the regeneration of cartilage-like tissue and neoformation of osteochondral tissue.
Article
Polymer Science
Alejandro Hernandez-Sosa, Rosa Ana Ramirez-Jimenez, Luis Rojo, Fouzia Boulmedais, Maria Rosa Aguilar, Miryam Criado-Gonzalez, Rebeca Hernandez
Summary: This study optimized printable alginate-cellulose ink formulations and successfully fabricated 3D-printed scaffolds with excellent biological functionality. The incorporation of the cell-adhesive peptide Fmoc-FFY improved cell-material adhesion. The scaffolds showed no cytotoxicity to osteosarcoma cells and inhibited their growth, while promoting adhesive interactions with the material.
Article
Polymer Science
Alejandra Rubio Hernandez-Sampelayo, Rodrigo Navarro, Dulce Maria Gonzalez-Garcia, Luis Garcia-Fernandez, Rosa Ana Ramirez-Jimenez, Maria Rosa Aguilar, Angel Marcos-Fernandez
Summary: This study presents a series of non-toxic biodegradable and biocompatible polyurethanes containing p-aminobenzoate moieties. These polymers were characterized chemically and physically, and their properties were influenced by the molecular weight of the soft segment and the crystallinity of the hard segment. The use of different chain extenders also affected the performance of the polyurethanes. The results showed good cell adhesion and cytotoxicity of human osteoblasts on the surfaces of these non-toxic biodegradable polyurethanes.
Review
Pharmacology & Pharmacy
Carmen Velasco-Salgado, Gloria Maria Pontes-Quero, Luis Garcia-Fernandez, Maria Rosa Aguilar, Kyra de Wit, Blanca Vazquez-Lasa, Luis Rojo, Cristina Abradelo
Summary: Osteoarthritis is a common joint disease characterized by cartilage degradation, subchondral bone thickening, and synovitis. Regenerative medicine strategies that aim to promote cartilage regeneration have become important in managing osteoarthritis. This review summarizes the latest research on the use of polymeric biomaterials for osteoarthritis management, which focus on restoring the viscoelastic properties of cartilage and reducing symptoms.
Article
Biochemistry & Molecular Biology
Daniel Fernandez-Villa, Rosa Ana Ramirez-Jimenez, Inmaculada Aranaz, Niuris Acosta, Blanca Vazquez-Lasa, Luis Rojo
Summary: This study synthesized a series of MTX complexes and characterized their physicochemical properties. The complexes showed new bioactive properties, such as increased deposition of GAGs and alternative anti-inflammatory capacities. Furthermore, the complexes were successfully loaded into spray-dried chitosan microparticles, enabling their encapsulation and localized delivery to rheumatoid arthritis-affected joints.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
Gerardo Asensio, Ana M. Hernandez-Arriaga, Marcela Martin-del-Campo, M. Auxiliadora Prieto, Luis Rojo, Blanca Vazquez-Lasa
Summary: This paper focuses on the synthesis and structural assessment of two metallic phytate complexes and their synergistic biological properties with phytic acid. The results show that these complexes significantly inhibit biofilm formation of Streptococcus mutans, with zinc phytate demonstrating remarkable differences compared to phytic acid and strontium phytate. These findings contribute to expanding the applications of bioactive metallic complexes in biotechnological and biomedical fields, particularly in dentistry.
SCIENTIFIC REPORTS
(2022)
Article
Chemistry, Physical
Gerardo Asensio, Ana M. Hernandez-Arriaga, Marcela Martin-del-Campo, M. Auxiliadora Prieto, Agustin R. Gonzalez-Elipe, Luis Rojo, Blanca Vazquez-Lasa
Summary: The accumulation of bacteria over implant surfaces is the main cause of failure, and the development of antimicrobial surfaces is crucial. Additionally, the durability and mechanical performance of implants are determined by their osseointegration capacity and infection prevention. Therefore, bioactive surfaces are being researched to improve implant osseointegration and infection prophylaxis.
APPLIED SURFACE SCIENCE
(2023)
Article
Engineering, Biomedical
Norbert Halfter, Eva Espinosa-Cano, Gloria Maria Pontes-Quero, Rosa Ana Ramirez-Jimenez, Christiane Heinemann, Stephanie Moeller, Matthias Schnabelrauch, Hans-Peter Wiesmann, Vera Hintze, Maria Rosa Aguilar
Summary: The limitations of current wound dressings for chronic wounds require the development of new approaches. One promising approach is the immune-centered approach, which aims to restore the regenerative and anti-inflammatory characteristics of macrophages. Ketoprofen nanoparticles (KT NPs) have shown potential in reducing inflammation and increasing anti-inflammatory cytokines in macrophages. This study investigated the incorporation of KT NPs into hyaluronan/collagen-based gels and their effects on cell viability, gel morphology, and inflammatory markers. The results suggest that HA/collagen-based gels containing KT NPs could be a novel therapeutic approach for chronic wounds, but further testing is needed to determine their efficacy in skin regeneration.
JOURNAL OF FUNCTIONAL BIOMATERIALS
(2023)
Article
Chemistry, Inorganic & Nuclear
Albert Rosado, Oriol Vallcorba, Blanca Vazquez-Lasa, Luis Garcia-Fernandez, Rosa Ana Ramirez-Jimenez, Maria Rosa Aguilar, Ana M. Lopez-Periago, Concepcion Domingo, Jose A. Ayllon
Summary: A new bioMOF called CaSyr-1 is prepared using an ethanol-based strategy, with features of full biocompatibility, high surface area (>1000 m(2) g(-1)), and a micropore diameter close to mesopore values. This bioMOF shows reversible adsorption of CO2 and can encapsulate bioactive ingredients, enabling the development of triple therapeutic entities involving Ca2+, syringic acid, and a drug.
INORGANIC CHEMISTRY FRONTIERS
(2023)