Article
Agricultural Engineering
Vasiliki Liava, Anestis Karkanis, Nikolaos Tsiropoulos
Summary: The study found that nitrogen fertilization significantly influenced the growth and fruit yield of milk thistle, with high nitrogen levels leading to increased fruit yield. However, nitrogen fertilization did not impact the accumulation of oil and silymarin in the fruits, although it did affect oil and silymarin yield.
INDUSTRIAL CROPS AND PRODUCTS
(2021)
Article
Biotechnology & Applied Microbiology
Purificacion Corchete, Lorena Almagro, Jose Antonio Gabaldon, Maria Angeles Pedreno, Javier Palazon
Summary: This study investigates the use of reusable CD polymers coated with magnetic Fe3O4 nanoparticles to enhance the production of bioactive compounds in transgenic cell cultures. The results show that HPCD-EPI-MN treatment significantly increases the accumulation of target products, but high concentrations and prolonged treatment have adverse effects on cell growth and viability. The polymer exhibits good stability and reusability within three elicitation cycles.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Review
Plant Sciences
Ilias Marmouzi, Abdelhakim Bouyahya, Shahira M. Ezzat, Meryem El Jemli, Mourad Kharbach
Summary: Milk thistle is a widely studied medicinal plant used for liver disorders, with its main active compound silymarin being commonly used as a herbal supplement. Despite numerous ethnopharmacological, experimental, and clinical investigations, its clinical efficacy remains uncertain, requiring further studies to validate its benefits.
JOURNAL OF ETHNOPHARMACOLOGY
(2021)
Article
Food Science & Technology
Luming Jia, Fei Zhao
Summary: This study evaluated the anti-fatigue properties of silymarin and found that it can decrease blood lactate and ammonia levels, increase muscle and liver glycogen content, and reduce creatine kinase activity after exercise. In addition, the observation of skeletal muscle structures confirmed that silymarin treatment resulted in less muscle damage compared to the control group after endurance exercise.
FOOD SCIENCE AND HUMAN WELLNESS
(2022)
Article
Agricultural Engineering
Magda Jablonowska, Petar Ciganovic, Jasna Jablan, Eva Margui, Michal Tomczyk, Marijana Zovko Koncic
Summary: This study compared the efficiency of maceration using glycerol/water mixtures with ethanol/water maceration for extracting silymarin from Silybum marianum fruit. The results showed that ultrasonication significantly improved extraction efficiency, with glycerol extraction resulting in a silymarin content similar to that of ethanol extraction.
INDUSTRIAL CROPS AND PRODUCTS
(2021)
Article
Food Science & Technology
Ahlem Ikhlef, Hamid Ait-Amar, Lakhdar Khelifi, Smain Chemat
Summary: In this study, different elicitors were used to treat plant cell cultures of Silybum marianum L. It was found that yeast extract was the most effective elicitor in enhancing the accumulation of silybin.
JOURNAL OF FOOD PROCESSING AND PRESERVATION
(2022)
Article
Plant Sciences
Tommaso Martinelli, Flavia Fulvio, Marco Pietrella, Laura Bassolino, Roberta Paris
Summary: This study investigated two contrasting chemotypes of S. marianum and revealed that chemotype differentiation in S. marianum is a metabolically regulated process driven by a monogenic heritable factor involved in silydianin biosynthesis. Transcriptome sequencing of the fruit shell during active silymarin accumulation identified dirigent-like transcripts as the main difference between the two chemotypes, suggesting their possible role in chemotype differentiation process.
JOURNAL OF APPLIED RESEARCH ON MEDICINAL AND AROMATIC PLANTS
(2023)
Article
Food Science & Technology
Maryam Aziz, Farhan Saeed, Nazir Ahmad, Aftab Ahmad, Muhammad Afzaal, Shahzad Hussain, Abdellatif A. Mohamed, Mohamed S. Alamri, Faqir Muhammad Anjum
Summary: The study evaluated the antioxidant potential and nutritional composition of milk thistle, highlighting significant differences in composition among different varieties and locations. Blue variety outperformed White in terms of nutrition, while Islamabad was the best location based on the results.
FOOD SCIENCE & NUTRITION
(2021)
Article
Pharmacology & Pharmacy
Meltem Macit, Gulengul Duman, Alev Cumbul, Engin Sumer, Caglar Macit
Summary: In this study, silymarin-nanoparticles were developed and their ability to reduce acetaminophen-induced toxicity was evaluated in vitro and in vivo. The nanoparticles showed hepatoprotective effects by reducing serum marker enzymes, as confirmed by histopathological studies. The nano approaches have the potential to enhance the therapeutic efficacy of silymarin. The silymarin-nanoparticles had a spherical shape with an average diameter between 138.9 nm and 1155 nm, and a zeta potential of -0.0340 mV. The average loading efficiency was approximately 32 +/- 0.5%.
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Fadia El Sherif, Yun-Kiam Yap, Sarah Alamer, Duaa Althumairy, Salah Khattab
Summary: This study aimed to promote plant growth and enhance the production of silybin (A + B) in Silybum marianum seeds by irradiating them with a He-Ne laser at 632.8 nm. The results showed that all laser seed treatments increased plant growth, with the highest yield observed in the 10-min treatment. Plants from the 6-, 8-, and 10-min laser-irradiated seeds had increased fruit silybin (A + B) content and chalcone synthase gene expressions. The highest silybin (A + B) contents were observed in plants from the 6-min-irradiated seeds, and the corresponding fruit extract exhibited elevated cell growth inhibition.
APPLIED SCIENCES-BASEL
(2023)
Article
Biochemistry & Molecular Biology
Dina Gad, Hamed El-Shora, Daniele Fraternale, Elisa Maricchiolo, Andrea Pompa, Karl-Josef Dietz
Summary: The enzymatic potential of Silybum marianum leaves to bioconvert phenolic acids into silymarin derivatives as chemopreventive agent was demonstrated in this study, with medium leaves showing the highest conversion activity.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Agriculture, Dairy & Animal Science
Hana Dockalova, Ladislav Zeman, Pavel Horky
Summary: The study found that feeding milk thistle seed cakes can have positive effects on the health of horses, especially in terms of liver diseases and antioxidant effects. Significant differences were detected in the biochemical markers of the experimental group, indicating the potential benefits of milk thistle in equine nutrition.
Article
Agronomy
Roberto Marceddu, Lucia Dinolfo, Alessandra Carrubba, Mauro Sarno, Giuseppe Di Miceli
Summary: Milk thistle is a versatile crop with reliable source of silymarin. Although there have been many studies on its bioactivity, phytochemistry, and genetics, research on its agronomy and farm opportunities is limited. However, its multiple potential uses and reduced need for external inputs make it an interesting alternative crop, especially for marginal environments.
Article
Multidisciplinary Sciences
Noreen Zahra, Abdul Wahid, Muhammad Bilal Hafeez, Mohammed Nasser Alyemeni, Tariq Shah, Parvaiz Ahmad
Summary: Silybum marianum (L.) Gaertn is a well-known medicinal plant, mainly used for silymarin content and oil production. This study focused on analyzing the Sily content in milk thistle ecotypes from different regions under salt stress, and the application of plant growth promoters showed positive effects on alleviating the deleterious effects of salinity stress and improving the quality and quantity parameters of milk thistle. Ecotypic variations with altered achene color patterns proved to be advantageous for higher Sily extraction under salt-stressed conditions.
SCIENTIFIC REPORTS
(2021)
Article
Biotechnology & Applied Microbiology
Muhammad aamir Shehzad, Mubarak Ali Khan, Amir Ali, Sher Mohammad, Ahmed Noureldeen, Hadeer Darwish, Asif Ali, Ayaz Ahmad, Tariq Khan, Raham Sher Khan
Summary: The study demonstrated that Zinc Oxide nanoparticles have a positive impact on callus growth and silymarin biosynthesis in milk thistle, enhancing secondary metabolite accumulation and antioxidant system activity. This research provides a standardized protocol for industrial production of silymarin through the use of ZnO NPs.
ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Juan C. Arevalo, Enrique Hernandez-Jimenez, Ada Jimenez-Gonzalez, Maria Torres-Valle, Roman S. Iwasaki, Roger Lopez-Bellido, Cristina Vicente-Garcia, Raquel E. Rodriguez
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2018)
Article
Neurosciences
Saray Lopez-Benito, Julia Sanchez-Sanchez, Veronica Brito, Laura Calvo, Silvia Lisa, Maria Torres-Valle, Mary E. Palko, Cristina Vicente-Garcia, Seila Fernandez-Fernandez, Juan P. Bolanos, Silvia Gines, Lino Tessarollo, Juan C. Arevalo
JOURNAL OF NEUROSCIENCE
(2018)
Article
Biochemistry & Molecular Biology
Judit Serrat, Maria Torres-Valle, Marta Lopez-Garcia, David Becerro-Recio, Mar Siles-Lucas, Javier Gonzalez-Miguel
Summary: Fasciola hepatica is a major causative agent of fasciolosis, a parasitic disease of growing public health concern. Understanding the interaction between F. hepatica and the host's intestinal extracellular matrix (ECM) can lead to the development of more effective control strategies against the disease.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Infectious Diseases
Judit Serrat, David Becerro-Recio, Maria Torres-Valle, Fernando Simon, Maria Adela Valero, Maria Dolores Bargues, Santiago Mas-Coma, Mar Siles-Lucas, Javier Gonzalez-Miguel
Summary: This study demonstrates that FhNEJ-Teg contains proteins that interact with plasminogen and stimulate its conversion into its active form, potentially facilitating trans-intestinal migration and successful establishment of the parasite within its mammalian host.
PLOS NEGLECTED TROPICAL DISEASES
(2023)
Article
Parasitology
David Becerro-Recio, Judit Serrat, Marta Lopez-Garcia, Maria Torres-Valle, Francisco Colina, Ivan M. Fernandez, Javier Gonzalez-Miguel, Mar Siles-Lucas
Summary: Fasciolosis is a widespread disease caused by the trematode parasite Fasciola hepatica, which has significant economic and veterinary impacts. It also poses a health risk to humans in endemic areas. The parasite's ability to adapt and evade the immune system allows it to survive in various ecosystems and hosts. The increasing prevalence rates of fasciolosis and the emergence of drug-resistant strains highlight the need for novel control measures. However, our understanding of the early stages of infection and the host response is still limited.
VETERINARY PARASITOLOGY
(2023)