Advances in chemical labeling of proteins in living cells

Near-instant surface-selective fluorogenic protein quantification using sulfonated triarylmethane dyes and fluorogen activating proteins

(2015) Qi Yan et al.   ORGANIC & BIOMOLECULAR CHEMISTRY

A Fluorogenic Probe for SNAP-Tagged Plasma Membrane Proteins Based on the Solvatochromic Molecule Nile Red

(2014) Efthymia Prifti et al.   ACS Chemical Biology

Multiplexed Modular Genetic Targeting of Quantum Dots

(2014) Saumya Saurabh et al.   ACS Nano

A Bifunctional Converter: Fluorescein Quenching scFv/Fluorogen Activating Protein for Photostability and Improved Signal to Noise in Fluorescence Experiments

(2014) Matthew J. Saunders et al.   BIOCONJUGATE CHEMISTRY

Fluorogen Activating Protein–Affibody Probes: Modular, No-Wash Measurement of Epidermal Growth Factor Receptors

(2014) Yi Wang et al.   BIOCONJUGATE CHEMISTRY

Genetic targeting of chemical indicators in vivo

(2014) Guoying Yang et al.   NATURE METHODS

Computational design of a red fluorophore ligase for site-specific protein labeling in living cells

(2014) D. S. Liu et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

A Fluorogenic TMP-Tag for High Signal-to-Background Intracellular Live Cell Imaging

(2013) Chaoran Jing et al.   ACS Chemical Biology

Localization Microscopy using Noncovalent Fluorogen Activation by Genetically Encoded Fluorogen-Activating Proteins.

(2013) Qi Yan et al.   CHEMPHYSCHEM

Malachite Green Mediates Homodimerization of Antibody VL Domains to Form a Fluorescent Ternary Complex with Singular Symmetric Interfaces

(2013) Chris Szent-Gyorgyi et al.   JOURNAL OF MOLECULAR BIOLOGY

Development of Fluorogenic Probes for Quick No-Wash Live-Cell Imaging of Intracellular Proteins

(2013) Yuichiro Hori et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Bipartite Tetracysteine Display Reveals Allosteric Control of Ligand-Specific EGFR Activation

(2012) Rebecca A. Scheck et al.   ACS Chemical Biology

Fast, Cell-Compatible Click Chemistry with Copper-Chelating Azides for Biomolecular Labeling

(2012) Chayasith Uttamapinant et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Genetically Encoded pH Sensor for Tracking Surface Proteins through Endocytosis

(2012) Anmol Grover et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Genetically Targetable and Color-Switching Fluorescent Probe

(2012) Dmytro A. Yushchenko et al.   CHEMBIOCHEM

Inhibitor Mediated Protein Degradation

(2012) Marcus J.C. Long et al.   CHEMISTRY & BIOLOGY

Fluorophore Targeting to Cellular Proteins via Enzyme-Mediated Azide Ligation and Strain-Promoted Cycloaddition

(2012) Jennifer Z. Yao et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Second-Generation Covalent TMP-Tag for Live Cell Imaging

(2012) Zhixing Chen et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Fluorescence Resonance Energy Transfer-Based Intracellular Assay for the Conformation of Hepatitis C Virus Drug Target NS5A

(2012) D. Bhattacharya et al.   JOURNAL OF VIROLOGY

Multicolor Single Molecule Tracking of Stochastically Active Synthetic Dyes

(2012) Alexander Benke et al.   NANO LETTERS

Key Physiological Parameters Dictate Triggering of Activity-Dependent Bulk Endocytosis in Hippocampal Synapses

(2012) Eva M. Wenzel et al.   PLoS One

A FlAsH-Based Cross-Linker to Study Protein Interactions in Living Cells

(2011) Anna Rutkowska et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Purification of Tetracysteine-Tagged Proteins by Affinity Chromatography Using a Non-Fluorescent, Photochemically Stable Bisarsenical Affinity Ligand

(2011) Lai-Qiang Ying et al.   BIOCONJUGATE CHEMISTRY

A FlAsH-Tetracysteine Assay for Quantifying the Association and Orientation of Transmembrane α-Helices

(2011) Christopher J. Pace et al.   CHEMBIOCHEM

Development of SNAP-Tag Fluorogenic Probes for Wash-Free Fluorescence Imaging

(2011) Xiaoli Sun et al.   CHEMBIOCHEM

Genetically Encodable Fluorescent Biosensors for Tracking Signaling Dynamics in Living Cells

(2011) Robert H. Newman et al.   CHEMICAL REVIEWS

Labelling cell structures and tracking cell lineage in zebrafish using SNAP-tag

(2011) Cláudia Campos et al.   DEVELOPMENTAL DYNAMICS

Semisynthesis of Fluorescent Metabolite Sensors on Cell Surfaces

(2011) Matthias A. Brun et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Cell Compatible Trimethoprim-Decorated Iron Oxide Nanoparticles Bind Dihydrofolate Reductase for Magnetically Modulating Focal Adhesion of Mammalian Cells

(2011) Marcus J. C. Long et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Small-molecule hydrophobic tagging–induced degradation of HaloTag fusion proteins

(2011) Taavi K Neklesa et al.   Nature Chemical Biology

Amyloid-β forms fibrils by nucleated conformational conversion of oligomers

(2011) Jiyong Lee et al.   Nature Chemical Biology

Defining the geometry of the two-component proteasome degron

(2011) Tomonao Inobe et al.   Nature Chemical Biology

Ordered and Dynamic Assembly of Single Spliceosomes

(2011) A. A. Hoskins et al.   SCIENCE

A Tale of Two Stomata

(2011) P. J. Hines   Science Signaling

Localizable and Highly Sensitive Calcium Indicator Based on a BODIPY Fluorophore

(2010) Mako Kamiya et al.   ANALYTICAL CHEMISTRY

Direct Visualization of Protein Association in Living Cells with Complex-Edited Electron Microscopy

(2010) Rachel J. Dexter et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Site-Specific, Orthogonal Labeling of Proteins in Intact Cells with Two Small Biarsenical Fluorophores

(2010) Alexander Zürn et al.   BIOCONJUGATE CHEMISTRY

Four-color single-molecule fluorescence with noncovalent dye labeling to monitor dynamic multimolecular complexes

(2010) Vanessa C. DeRocco et al.   BIOTECHNIQUES

Visualizing Tyrosine Kinase Activity with Bipartite Tetracysteine Display

(2010) Sarmistha Ray-Saha et al.   CHEMBIOCHEM

Fluorogen-activating proteins as biosensors of cell-surface proteins in living cells

(2010) John Holleran et al.   CYTOMETRY PART A

Cytoskeletal coherence requires myosin-IIA contractility

(2010) Y. Cai et al.   JOURNAL OF CELL SCIENCE

Fluorogenic Dendrons with Multiple Donor Chromophores as Bright Genetically Targeted and Activated Probes

(2010) Christopher Szent-Gyorgyi et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Superresolution Imaging of Targeted Proteins in Fixed and Living Cells Using Photoactivatable Organic Fluorophores

(2010) Hsiao-lu D. Lee et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Biocompatible Copper(I) Catalysts for in Vivo Imaging of Glycans

(2010) David Soriano del Amo et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Live-cell super-resolution imaging with trimethoprim conjugates

(2010) Richard Wombacher et al.   NATURE METHODS

Fluorescent labeling of tetracysteine-tagged proteins in intact cells

(2010) Carsten Hoffmann et al.   Nature Protocols

Fura-2FF-based calcium indicator for protein labeling

(2010) Agostina A. Ruggiu et al.   ORGANIC & BIOMOLECULAR CHEMISTRY

Blue fluorescent dye-protein complexes based on fluorogenic cyanine dyes and single chain antibody fragments

(2010) Kimberly J. Zanotti et al.   ORGANIC & BIOMOLECULAR CHEMISTRY

Time-resolved luminescence resonance energy transfer imaging of protein-protein interactions in living cells

(2010) H. E. Rajapakse et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

A fluorophore ligase for site-specific protein labeling inside living cells

(2010) C. Uttamapinant et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Indo-1 Derivatives for Local Calcium Sensing

(2009) Michael Bannwarth et al.   ACS Chemical Biology

AnIn VivoCovalent TMP-Tag Based on Proximity-Induced Reactivity

(2009) Sarah S. Gallagher et al.   ACS Chemical Biology

In VivoStable Tumor-Specific Painting in Various Colors Using Dehalogenase-Based Protein-Tag Fluorescent Ligands

(2009) Nobuyuki Kosaka et al.   BIOCONJUGATE CHEMISTRY

STED Nanoscopy in Living Cells Using Fluorogen Activating Proteins

(2009) James A. J. Fitzpatrick et al.   BIOCONJUGATE CHEMISTRY

In Vivo Labeling Method Using a Genetic Construct for Nanoscale Resolution Microscopy

(2009) Jan Schröder et al.   BIOPHYSICAL JOURNAL

Bipartite Tetracysteine Display Requires Site Flexibility for ReAsH Coordination

(2009) Jessica L. Goodman et al.   CHEMBIOCHEM

Site-specific covalent labeling of His-tag fused proteins with a reactive Ni(ii)–NTA probe

(2009) Sho-hei Uchinomiya et al.   CHEMICAL COMMUNICATIONS

Hairpin Structure of a Biarsenical−Tetracysteine Motif Determined by NMR Spectroscopy

(2009) Fatemeh Madani et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Selective Cross-Linking of Interacting Proteins Using Self-Labeling Tags

(2009) Arnaud Gautier et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Semisynthetic Fluorescent Sensor Proteins Based on Self-Labeling Protein Tags

(2009) Matthias A. Brun et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Photoactive Yellow Protein-Based Protein Labeling System with Turn-On Fluorescence Intensity

(2009) Yuichiro Hori et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Yeast Display Evolution of a Kinetically Efficient 13-Amino Acid Substrate for Lipoic Acid Ligase

(2009) Sujiet Puthenveetil et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Enhanced Photostability of Genetically Encodable Fluoromodules Based on Fluorogenic Cyanine Dyes and a Promiscuous Protein Partner

(2009) Nathaniel I. Shank et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Biarsenical Labeling of Vesicular Stomatitis Virus Encoding Tetracysteine-Tagged M Protein Allows Dynamic Imaging of M Protein and Virus Uncoating in Infected Cells

(2009) S. C. Das et al.   JOURNAL OF VIROLOGY

Site-specific chemical modification of recombinant proteins produced in mammalian cells by using the genetically encoded aldehyde tag

(2009) P. Wu et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Selective translocation of intracellular Smoothened to the primary cilium in response to Hedgehog pathway modulation

(2009) Yu Wang et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Peroxisome Dynamics in Cultured Mammalian Cells

(2009) Sofie J. Huybrechts et al.   TRAFFIC

HaloTag: A Novel Protein Labeling Technology for Cell Imaging and Protein Analysis

(2008) Georgyi V. Los et al.   ACS Chemical Biology

An Engineered Aryl Azide Ligase for Site-Specific Mapping of Protein-Protein Interactions through Photo-Cross-Linking

(2008) Hemanta Baruah et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Cross-Strand Split Tetra-Cys Motifs as Structure Sensors in a β-Sheet Protein

(2008) Beena Krishnan et al.   CHEMISTRY & BIOLOGY

An Engineered Protein Tag for Multiprotein Labeling in Living Cells

(2008) Arnaud Gautier et al.   CHEMISTRY & BIOLOGY

Fluorescent labeling of proteins in living cells using the FKBP12 (F36V) tag

(2008) Matt Robers et al.   CYTOMETRY PART A

The cytoplasmic accumulations of the cataract-associated mutant, Connexin50P88S, are long-lived and form in the endoplasmic reticulum

(2008) Alexandra Lichtenstein et al.   EXPERIMENTAL EYE RESEARCH

Inactivation of clathrin heavy chain inhibits synaptic recycling but allows bulk membrane uptake

(2008) Jaroslaw Kasprowicz et al.   JOURNAL OF CELL BIOLOGY

Exploiting the Nitrilotriacetic Acid Moiety for Biolabeling with Ultrastable Perylene Dyes

(2008) Kalina Peneva et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

An Eight Residue Fragment of an Acyl Carrier Protein Suffices for Post-Translational Introduction of Fluorescent Pantetheinyl Arms in Protein Modificationin vitroandin vivo

(2008) Zhe Zhou et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Expanding the Substrate Tolerance of Biotin Ligase through Exploration of Enzymes from Diverse Species

(2008) Sarah A. Slavoff et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

A Rainbow of Fluoromodules: A Promiscuous scFv Protein Binds to and Activates a Diverse Set of Fluorogenic Cyanine Dyes

(2008) Crystal Lee Pow et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Selective Recognition of Protein Tetraserine Motifs with a Cell-Permeable, Pro-fluorescent Bis-boronic Acid

(2008) Tiffany L. Halo et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Imaging proteins in live mammalian cells with biotin ligase and monovalent streptavidin

(2008) Mark Howarth et al.   Nature Protocols

Identification of Six New Photoactive Yellow ProteinsDiversity and StructureFunction Relationships in a Bacterial Blue Light Photoreceptor

(2008) Masato Kumauchi et al.   PHOTOCHEMISTRY AND PHOTOBIOLOGY