Article
Polymer Science
Milena B. Boarini, Natalia S. Gsponer, Maria E. Milanesio, Edgardo N. Durantini
Summary: This study synthesized two novel polymeric conjugates that exhibited significant photodynamic effects and tested their photoinactivation abilities against Staphylococcus aureus and Escherichia coli.
EUROPEAN POLYMER JOURNAL
(2023)
Review
Food Science & Technology
Lu Chen, Yong Zhao, Weiliang Wu, Qiaohui Zeng, Jing Jing Wang
Summary: This review provides a comprehensive overview of the bactericidal ability of photodynamic inactivation (PDI) mediated by endogenous and exogenous photosensitizers against planktonic bacteria, biofilms, and fungi. Efforts to strengthen PDI potency in the food system, including physical and chemical modification of photosensitizers, combination with adjuvants, and improving targeting ability are discussed. The mechanisms of PDI on eradicating biofilms and preserving foods, as well as the concept of PDI-mediated antimicrobial packaging film are also summarized.
COMPREHENSIVE REVIEWS IN FOOD SCIENCE AND FOOD SAFETY
(2023)
Article
Chemistry, Physical
Qian Wang, Dandan Zhang, Jin Feng, Tingli Sun, Cailing Li, Xiaobao Xie, Qingshan Shi
Summary: In this study, a chlorine-loaded polyethyleneimine-based micelle photosensitizer was constructed for enhanced photoinactivation activities on Gram-negative bacteria through electrostatic and hydrophobic interactions between nanoparticles and the bacterial surface. The light-triggered generation of reactive oxygen species caused irreversible damage to the bacterial outer membrane, achieving enhanced bactericidal efficiency. This antimicrobial alternative holds great potential for human healthcare and environmental remediation.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Yuanyuan Yan, Lijun Tan, Huihui Li, Bowen Chen, Jiaming Huang, Yong Zhao, Jingjing Wang, Jie Ou
Summary: Photodynamic inactivation (PDI) using blue light LED and the photosensitizer SMC is an effective method to eradicate Staphylococcus aureus in food. The study found that PDI is potent in killing S. aureus on lettuce and can also prevent weight loss and color changes during storage.
PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES
(2021)
Article
Multidisciplinary Sciences
Hilde Harb Buzza, Fernanda Alves, Ana Julia Barbosa Tome, Juan Chen, Giulia Kassab, Jiachuan Bu, Vanderlei Salvador Bagnato, Gang Zheng, Cristina Kurachi
Summary: NewPS-aPDT is a safe and effective antimicrobial photosensitizer against biofilms. In experiments, NewPS showed high bactericidal efficacy in suspensions of Streptococcus pneumoniae and Staphylococcus aureus, and enhanced antibacterial effect in S. aureus biofilms with a higher concentration of 100 mu M, longer incubation time, and higher light dose. In a murine-infected wound model, NewPS-aPDT demonstrated better therapeutic efficacy and faster tissue healing compared to other treatment groups.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Biophysics
Amanda Cristina Zangirolami, Fernanda Carbinatto, Jose Dirceu Vollet Filho, Vanderlei Salvador Bagnato, Kate Cristina Blanco
Summary: The study demonstrates that curcuminoids and Photogem formulations combined with light illumination can effectively inhibit the formation and infection of bacterial biofilms. Curcuminoid formulations show better inhibition of Staphylococcus aureus biofilms, while Photogem formulations show better inhibition of Escherichia coli biofilms.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2022)
Article
Biotechnology & Applied Microbiology
Fatima Shatila, Grace M. O. Tieman, Stefania F. Musolino, Jeremy E. Wulff, Heather L. Buckley
Summary: The study examined the antimicrobial effect of cationic porphyrin-functionalized polyethylene terephthalate (PET) discs against E. coli and Pseudomonas aeruginosa growth. The results showed a significant reduction in planktonic cells and biofilm formation of the bacteria, highlighting the potential of porphyrin-functionalized photoactive surfaces as a means to prevent biofilm formation.
INTERNATIONAL BIODETERIORATION & BIODEGRADATION
(2023)
Article
Infectious Diseases
Sakari Nikinmaa, Anna Podonyi, Peter Raivio, Jukka Meurman, Timo Sorsa, Juha Rantala, Esko Kankuri, Tuomas Tauriainen, Tommi Paetilae
Summary: Dual-light aPDT shows improved bactericidal effect on Staphylococcus aureus biofilm compared to aPDT or aBL, providing a sustained effect. Increasing the relative ratio of aBL enhances the antibacterial effect, especially with repeated applications.
Article
Microbiology
Shanghong Liu, Yanpeng Xiong, Haitao Xiao, Jinxin Zheng, Zewen Wen, Duoyun Li, Qiwen Deng, Zhijian Yu
Summary: In this study, the antibacterial and excellent anti-biofilm activity of entrectinib against S. aureus was demonstrated. Entrectinib also exhibited good safety, showed the reduction in bacterial burden, and had an impact on protein biosynthesis and oxidative stress. Furthermore, the bactericidal action of entrectinib on S. aureus was confirmed by disrupting the bacterial cell membrane.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Acoustics
Jinyue Sun, Debao Wang, Zhilan Sun, Fang Liu, Lihui Du, Daoying Wang
Summary: The study demonstrated that the combined treatment of ultrasound and chlorogenic acid exhibited significant synergistic antibacterial effects against Staphylococcus aureus, causing severe cell damage, decreased membrane permeability, release of intracellular substances, and reduced exopolysaccharide content in bacterial biofilms.
ULTRASONICS SONOCHEMISTRY
(2021)
Article
Chemistry, Physical
Nanxin Zhao, Rongfeng Cai, Yuting Zhang, Xiaoli Wang, Nandi Zhou
Summary: Drug-resistant bacteria, especially those in microbial biofilm, pose a significant threat to human health. Traditional antibiotic treatment is not effective in sterilizing biofilm-associated pathogens, thus new approaches are urgently needed. In this study, researchers developed a pH-gated photosensitizer delivery system using amino-modified hollow mesoporous silica nanoparticles. The system efficiently targeted and released the photosensitizer curcumin into the biofilm, and subsequent photodynamic treatment significantly reduced the number of viable bacteria. This new approach shows promise in combating biofilm-associated infections without the risk of antibiotic resistance.
Article
Food Science & Technology
Sarah H. I. Lee, Antje Froehling, Oliver Schlueter, Carlos H. Corassin, Elaine C. P. De Martinis, Virginia F. Alves, Tatiana C. Pimentel, Carlos A. F. Oliveira
Summary: The study found that peracetic acid (PAA) had a better bactericidal effect on Staphylococcus aureus and Listeria monocytogenes in a short period of time compared to cold atmospheric pressure plasma (CAPP). CAPP showed a mild bactericidal effect on these two bacteria strains, with no significant impact after 120 seconds.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2021)
Article
Food Science & Technology
Chaoyi Shen, Jiahao Zhong, Fengjun Guo, Changfeng Zhang, Changqing Zhu, Di Wu, Kunsong Chen
Summary: This study successfully fabricated CUR-loaded PCL/CMCH nanofibers using solution blow spinning technique, which exhibited photodynamic inactivation activity for inactivating S. aureus. The nanofibers showed improved thermal stability, tensile strength, hydrophobicity, and sustained release of CUR. The developed nanofibers can be applied as food packaging materials to reduce the impact of S. aureus on human wellness.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Wei Yang, Ziyuan Wang, Qing Li, Yating Jia, Shuimiao Song, Zichu Ma, Jie Liu, Jing Wang
Summary: The study found that PDI using curcumin associated with LED can effectively prevent and disrupt the formation of Staphylococcus saprophyticus biofilms, including inducing biofilm prevention and removal, metabolic inactivation, intracellular membrane damage, and cell death.
Article
Biotechnology & Applied Microbiology
Yali Li, Yi Xu, Qiaoming Liao, Mengmeng Xie, Han Tao, Hui-Li Wang
Summary: This study demonstrated that the photodynamic efficacy of hypocrellin B can be potentiated by joint action with curcumin, leading to a synergistic activity in controlling the growth of Staphylococcal aureus. The synergistic action may be attributed to the increased type I/type II ratio of reactive oxygen species (ROS). The use of dual photosensitizers reduced microbial contamination of tested apple while maintaining its quality, providing a novel approach to food safety.
MICROBIAL BIOTECHNOLOGY
(2021)
Article
Pharmacology & Pharmacy
Gabriela Cervini Bohm, Lautaro Gandara, Gabriela Di Venosa, Leandro Mamone, Fernanda Buzzola, Adriana Casas
BIOCHEMICAL PHARMACOLOGY
(2020)
Article
Biochemistry & Molecular Biology
Maria Laura Mugas, Gustavo Calvo, Juliana Marioni, Mariela Cespedes, Florencia Martinez, Daniel Saenz, Gabriela Di Venosa, Jose L. Cabrera, Susana Nunez Montoya, Adriana Casas
Summary: PTN isolated from the lichen Teoloschistes nodulifer showed promising potential as a photosensitiser for PDT in treating superficial malignancies. The uptake of PTN by tumor cells mainly through passive diffusion and significant cell death under blue light irradiation were observed.
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY
(2021)
Article
Agronomy
Maria C. Perez-Piza, Pablo E. Grijalba, Ezequiel Cejas, Juan C. Chamorro Garces, Matias Ferreyra, Carla Zilli, Pablo Vallecorsa, Diego Santa-Cruz, Gustavo Yannarelli, Leandro Prevosto, Karina Balestrasse
Summary: Exposure of D. longicolla colonies to NTP severely compromised fungal biology, with negative effects on fungal growth, biomass, and colony appearance. Ozone production during treatment and lipid peroxidation of fungal cell membranes appeared to be involved in the observed effects. Asexual spores (conidia) of the fungi were also highly susceptible to NTP exposure.
PEST MANAGEMENT SCIENCE
(2021)
Article
Chemistry, Applied
Cristian M. Pis Diez, Mariela Cespedes, Gabriela M. Di Venosa, Gustavo Calvo, Esteban Avigliano, Adriana G. Casas, Jorge A. Palermo
Summary: In this study, eleven new derivatives of the alkaloid olivacine were synthesized, showing increased cytotoxicity towards cell lines and some exhibiting indications of lysosomal apoptosis. The subcellular localization of olivacine and its synthetic derivatives was studied, revealing a different profile of cell distribution among the phases of the cycle for some of the new compounds compared to olivacine.
NATURAL PRODUCT RESEARCH
(2022)
Article
Multidisciplinary Sciences
Cristian Dotto, Andrea Lombarte Serrat, Martin Ledesma, Carlos Vay, Monika Ehling-Schulz, Daniel O. Sordelli, Tom Grunert, Fernanda Buzzola
Summary: The study demonstrated that salicylic acid impairs biofilm dispersal of Staphylococcus aureus by interfering with agr expression, leading to reduced production of protease and surfactant molecules, as well as negative effects on bacterial cell autolysis. It also revealed the lack of extracellular DNA in the biofilm matrix. Additionally, in silico docking and simulation suggested a potential interaction between AgrA and SAL, resulting in reduced activity of the agr system.
SCIENTIFIC REPORTS
(2021)
Article
Oncology
Pablo Vallecorsa, Gabriela Di Venosa, M. Belen Ballatore, Dario Ferreyra, Leandro Mamone, Daniel Saenz, Gustavo Calvo, Edgardo Durantini, Adriana Casas
Summary: The study evaluated the photoactivity of four meso-substituted porphyrins and a porphyrin coupled to a fullerene in vitro. The PS with the best photokilling activity was administered to mice bearing the LM3 subcutaneously implanted adenocarcinoma. The results showed high photoactivity against LM3 mammary carcinoma cells for TAPP, DAPP, TEMCC4+ and TEMCP4+, with TAPP being the most active.
Article
Biochemistry & Molecular Biology
Pablo Vallecorsa, Gabriela Di Venosa, Gabriel Gola, Daniel Saenz, Leandro Mamone, Alexander J. MacRobert, Javier Ramirez, Adriana Casas
Summary: This study compared the efficacy of newly synthesized ALA esters in the treatment of cutaneous T-cell lymphomas, finding that some esters were more effective for photodynamic therapy than ALA. By comparing the use of different ALA esters, it was discovered that the concentrations of these esters were 4-25 times lower than ALA, showing potential for application in the treatment of cutaneous T-cell lymphomas.
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY
(2021)
Article
Plant Sciences
M. C. Perez-Piza, V. N. Ibanez, A. Varela, E. Cejas, M. Ferreyra, J. C. Chamorro-Garces, C. Zilli, P. Vallecorsa, B. Fina, L. Prevosto, C. F. Marfil, K. B. Balestrasse
Summary: Non-thermal plasmas (NTP) show promise as a technology for seed treatment, with treated soybean seeds exhibiting superior growth compared to untreated seeds. Epigenetic changes induced by NTP treatments were observed, with both demethylation and methylation changes at specific loci potentially contributing to the phenotypic differences observed.
JOURNAL OF PLANT GROWTH REGULATION
(2022)
Article
Biochemistry & Molecular Biology
Maria E. Perez, Javier E. Durantini, Eugenia Reynoso, Maria G. Alvarez, Maria E. Milanesio, Edgardo N. Durantini
Summary: New porphyrin-Schiff base conjugates containing one (6) and two (7) basic amino groups were synthesized, allowing easier access to porphyrins substituted with positive charge precursor groups in aqueous media. These compounds showed red fluorescence emission and were able to photo-oxidize amino acids, with enhanced photodynamic inactivation capacity when combined with iodide anions. The presence of basic aliphatic amino groups in these porphyrins provided an amphiphilic character, improving their interaction with cell walls and photocytotoxic activity against microorganisms.
Article
Infectious Diseases
Sofia C. Santamarina, Daniel A. Heredia, Andres M. Durantini, Edgardo N. Durantini
Summary: The widespread use of antibiotics has led to an increase in microbial resistance, necessitating the development of new strategies to combat pathogens. Photoactive polymers offer an interesting approach to kill microbes and maintain aseptic surfaces.
Review
Biology
Mindy Liu Perkins, Lautaro Gandara, Justin Crocker
Summary: Identifying the general principles by which genotypes are converted into phenotypes remains a challenge. Synthetic biology techniques can facilitate a systematic exploration of evolvability across biological scales. Combining synthetic approaches with multi-level phenotyping can provide a detailed characterization of how stimuli shape the morphology and behavior of organisms. Integrating experimental data with mathematical and computational techniques is advocated to pursue a comprehensive theory of evolution.
PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES
(2022)
Article
Cell Biology
Rafael Galupa, Gilberto Alvarez-Canales, Noa Ottilie Borst, Timothy Fuqua, Lautaro Gandara, Natalia Misunou, Kerstin Richter, Mariana R. P. Alves, Esther Karumbi, Melinda Liu Perkins, Tin Kocijan, Christine A. Rushlow, Justin Crocker
Summary: Developmental enhancers bind transcription factors and dictate patterns of gene expression during development. Their molecular evolution can underlie phenotypical evolution, but the contributions of the evolutionary pathways involved remain little understood. Our findings suggest that the phenotypic landscapes of developmental enhancers are constrained by enhancer architecture and chromatin accessibility. The evolution of existing enhancers is limited in its capacity to generate novel phenotypes, whereas the activity of de novo elements is a primary source of phenotypic novelty.
DEVELOPMENTAL CELL
(2023)
Article
Cell Biology
Lautaro Gandara, Albert Tsai, Mans Ekeloef, Rafael Galupa, Ella Preger-Ben Noon, Theodore Alexandrov, Justin Crocker
Summary: This study investigates the effects of hypomethylation on gene expression, morphology, metabolism, behavior, and offspring production in Drosophila melanogaster. The results demonstrate that hypomethylation disrupts gene expression and reduces phenotypic robustness. However, these phenotypic changes are only observed in specific genetic backgrounds or outside of standard laboratory environments.
Article
Microbiology
Maria Emilia Caceres, Martin Manuel Ledesma, Andrea Lombarte Serrat, Carlos Vay, Daniel Oscar Sordelli, Onica Nancy Giacomodonato, Fernanda Roxana Buzzola
Summary: The study evaluated the effect of different growing culture conditions on the biofilm formation of clinical mastitis pathogens, with MALDI-TOF-MS proving to be an excellent tool for discriminating between different biofilm formation levels. Some strains showed increased biofilm formation in milk. Free iron medium and milk whey inhibited biofilm formation in most strains.
CURRENT RESEARCH IN MICROBIAL SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Girgis Obaid, Menitte Eroy, Jie Zhao, Shazia Bano, Mari Mino-Kenudson, Tayyaba Hasan
Summary: The presence of desmoplasia in pancreatic ductal adenocarcinoma (PDAC) is associated with poor treatment outcomes and reduced drug delivery. By analyzing collagen subtypes in PDAC tumors following different treatment regimens, immunofluorescence profiling can predict long-term survival and tumor growth inhibition. This information can aid in personalized treatment and improve patient survival.
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY
(2024)
Article
Biochemistry & Molecular Biology
Fatemeh Malekpour, Rashin Bahrami, Mahshid Hodjat, Neda Hakimiha, Behnam Bolhari, Aidin Sooratgar, Soheil Niavarzi
Summary: This study investigated the effect of photobiomodulation (PBM) therapy using an 808 nm diode laser on cellular modulation mechanisms in regenerative endodontic procedures (REPs). The results showed that PBM treatment significantly increased cell viability and migration, and enhanced the release of TGF-131 from dentin.
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY
(2024)
Article
Biochemistry & Molecular Biology
Pranathi Tata, Ramakrishnan Ganesan, Jayati Ray Dutta
Summary: Thin film coatings with potent antibacterial properties are widely used in medical devices, frequently touched surfaces, and food packaging. However, achieving antibacterial performance under both light and dark conditions remains a challenge. In this study, we successfully prepared AgBr-coated ZnO nanorod thin film architecture using a unique surfactant-mediated solution-phase spin-coating approach. The resulting structures showed dual functionality as antimicrobial agents and photosensitizers, effectively enhancing the visible-light photodisinfection efficacy of ZnO nanorods. The concentration of the surfactant-based precursor solution could be modulated to control the AgBr content. Additionally, components like urea and lactic acid were found to enhance the photocatalytic efficiency of the structures.
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY
(2024)