Loop pathways are responsible for tuning the accumulation of C19- and C22-sterol intermediates in the mycobacterial phytosterol degradation pathway

Efficient conversion of phytosterols into 4-androstene-3,17-dione and its C1,2-dehydrogenized and 9α-hydroxylated derivatives by engineered Mycobacteria

(2021) Xin Li et al.   Microbial Cell Factories

Mycolicibacterium cell factory for the production of steroid-based drug intermediates

(2021) Anqi Zhao et al.   BIOTECHNOLOGY ADVANCES

Production of 9,21-dihydroxy-20-methyl-pregna-4-en-3-one from phytosterols in Mycobacterium neoaurum by modifying multiple genes and improving the intracellular environment

(2021) Chen-Yang Yuan et al.   Microbial Cell Factories

The sterol carrier hydroxypropyl-β-cyclodextrin enhances metabolism of phytosterols by Mycobacterium neoaurum

(2020) Liqiu Su et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Synthesis of ursodeoxycholic acid from plant-source (20S)-21-hydroxy-20-methylpregn-4-en-3-one

(2020) Jie Wang et al.   STEROIDS

A recycled batch biotransformation strategy for 22-hydroxy-23,24-bisnorchol-4-ene-3-one production from high concentration of phytosterols by mycobacterial resting cells

(2020) Yu Hu et al.   BIOTECHNOLOGY LETTERS

Whole-genome and enzymatic analyses of an androstenedione-producing Mycobacterium strain with residual phytosterol-degrading pathways

(2020) Hongwei Wang et al.   Microbial Cell Factories

A Dual Role Reductase from Phytosterols Catabolism Enables the Efficient Production of Valuable Steroid Precursors

(2020) Haidong Peng et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Identification of bottlenecks in 4-androstene-3,17-dione/1,4-androstadiene-3,17-dione synthesis by Mycobacterium neoaurum JC-12 through comparative proteomics

(2020) Chao Liu et al.   JOURNAL OF BIOSCIENCE AND BIOENGINEERING

Metabolic engineering of microbial cell factories for production of nutraceuticals

(2019) Shuo-Fu Yuan et al.   Microbial Cell Factories

Unravelling a new catabolic pathway of C-19 steroids in Mycobacterium smegmatis

(2018) Lorena Fernández-Cabezón et al.   ENVIRONMENTAL MICROBIOLOGY

Identification, function, and application of 3-ketosteroid Δ1-dehydrogenase isozymes in Mycobacterium neoaurum DSM 1381 for the production of steroidic synthons

(2018) Ruijie Zhang et al.   Microbial Cell Factories

Microbial biotransformation of bioactive and clinically useful steroids and some salient features of steroids and biotransformation

(2018) Nighat Sultana   STEROIDS

New product identification in the sterol metabolism by an industrial strain Mycobacterium neoaurum NRRL B-3805

(2018) Xuemei Li et al.   STEROIDS

Metagenomes Reveal Global Distribution of Bacterial Steroid Catabolism in Natural, Engineered, and Host Environments

(2018) Johannes Holert et al.   mBio

New Insights on Steroid Biotechnology

(2018) Lorena Fernández-Cabezón et al.   Frontiers in Microbiology

Engineered 3-Ketosteroid 9α-Hydroxylases in Mycobacterium neoaurum : an Efficient Platform for Production of Steroid Drugs

(2018) Hao-Hao Liu et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Enhancing Expression of 3-Ketosteroid-9α-Hydroxylase Oxygenase, an Enzyme with Broad Substrate Range and High Hydroxylation Ability, in Mycobacterium sp. LY-1

(2018) Hui Li et al.   APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY

Overexpression of cytochrome p450 125 in Mycobacterium: a rational strategy in the promotion of phytosterol biotransformation

(2018) Liqiu Su et al.   JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY

Metabolic Adaptation of Mycobacterium neoaurum ATCC 25795 in the Catabolism of Sterols for Producing Important Steroid Intermediates

(2018) Min Liu et al.   JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

Improving the production of 22-hydroxy-23,24-bisnorchol-4-ene-3-one from sterols in Mycobacterium neoaurum by increasing cell permeability and modifying multiple genes

(2017) Liang-Bin Xiong et al.   Microbial Cell Factories

Cofactor engineering to regulate NAD+/NADH ratio with its application to phytosterols biotransformation

(2017) Liqiu Su et al.   Microbial Cell Factories

Efficient androst-1,4-diene-3,17-dione production by co-expressing 3-ketosteroid-Δ1-dehydrogenase and catalase inBacillus subtilis

(2016) M. Shao et al.   JOURNAL OF APPLIED MICROBIOLOGY

A mutant form of 3-ketosteroid-Δ1-dehydrogenase gives altered androst-1,4-diene-3, 17-dione/androst-4-ene-3,17-dione molar ratios in steroid biotransformations by Mycobacterium neoaurum ST-095

(2016) Minglong Shao et al.   JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY

KEGG: new perspectives on genomes, pathways, diseases and drugs

(2016) Minoru Kanehisa et al.   NUCLEIC ACIDS RESEARCH

Sterol Synthesis in Diverse Bacteria

(2016) Jeremy H. Wei et al.   Frontiers in Microbiology

EngineeringMycobacterium smegmatisfor testosterone production

(2016) Lorena Fernández-Cabezón et al.   Microbial Biotechnology

Mycobacterium smegmatisis a suitable cell factory for the production of steroidic synthons

(2016) Beatriz Galán et al.   Microbial Biotechnology

Unraveling and engineering the production of 23,24-bisnorcholenic steroids in sterol metabolism

(2016) Li-Qin Xu et al.   Scientific Reports

Genetic differences in ksdD influence on the ADD/AD ratio of Mycobacterium neoaurum

(2015) Rili Xie et al.   JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY

3-Ketosteroid 9α-hydroxylase enzymes: Rieske non-heme monooxygenases essential for bacterial steroid degradation

(2014) Mirjan Petrusma et al.   ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY

Characterization and engineering of 3-ketosteroid-△1-dehydrogenase and 3-ketosteroid-9α-hydroxylase in Mycobacterium neoaurum ATCC 25795 to produce 9α-hydroxy-4-androstene-3,17-dione through the catabolism of sterols

(2014) Kang Yao et al.   METABOLIC ENGINEERING

Accumulation of androstadiene-dione by overexpression of heterologous 3-ketosteroid Δ1-dehydrogenase in Mycobacterium neoaurum NwIB-01

(2014) Wei Wei et al.   WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY

The Essential Function of Genes for a Hydratase and an Aldehyde Dehydrogenase for Growth of Pseudomonas sp. Strain Chol1 with the Steroid Compound Cholate Indicates an Aldolytic Reaction Step for Deacetylation of the Side Chain

(2013) J. Holert et al.   JOURNAL OF BACTERIOLOGY

Comparative analysis of genes encoding key steroid core oxidation enzymes in fast-growing Mycobacterium spp. strains

(2013) E.Yu. Bragin et al.   JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY

Microbial steroid transformations: current state and prospects

(2012) Marina V. Donova et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Identification and engineering of cholesterol oxidases involved in the initial step of sterols catabolism in Mycobacterium neoaurum

(2012) Kang Yao et al.   METABOLIC ENGINEERING

Conversion of phytosterols into androstenedione by Mycobacterium neoaurum

(2011) N. V. Rodina et al.   APPLIED BIOCHEMISTRY AND MICROBIOLOGY

Inactivation and Augmentation of the Primary 3-Ketosteroid- 1- Dehydrogenase in Mycobacterium neoaurum NwIB-01: Biotransformation of Soybean Phytosterols to 4-Androstene- 3,17-Dione or 1,4-Androstadiene-3,17-Dione

(2010) W. Wei et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

A New Steroid-Transforming Strain of Mycobacterium neoaurum and Cloning of 3-Ketosteroid 9α-Hydroxylase in NwIB-01

(2010) Wei Wei et al.   APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY

Androstenedione production by biotransformation of phytosterols

(2008) Alok Malaviya et al.   BIORESOURCE TECHNOLOGY

Enhancement of androstadienedione production from progesterone by biotransformation using the hydroxypropyl-β-cyclodextrin complexation technique

(2007) Aranya Manosroi et al.   JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY