Journal
JOURNAL OF OLEO SCIENCE
Volume 65, Issue 3, Pages 257-262Publisher
JAPAN OIL CHEMISTS SOC
DOI: 10.5650/jos.ess15207
Keywords
biodegradable plastic-degrading enzyme; Paraphoma; poly(butylene succinate-co-adipate)
Categories
Funding
- Science and Technology Research Promotion Program for Agriculture, Forestry, Fisheries and Food Industry [25017A]
- National Institute for Agro-Environmental Sciences, Japan
- Japan Society for the Promotion of Science (JSPS)
- JSPS KAKENHI [25.40169]
- Grants-in-Aid for Scientific Research [13J40169] Funding Source: KAKEN
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To improve the productivity of Paraphoma-like fungal strain B47-9 for biodegradable plastic (BP)-degrading enzyme (PCLE), the optimal concentration of emulsified poly(butylene succinate-co-adipate) (PBSA) in the medium was determined. Emulsified PBSA was consumed as a sole carbon source and an inducer of PCLE production by strain B47-9. Among the various concentrations of emulsified PBSA [0.09-0.9% (w/v)] used in flask cultivation, 0.27% yielded the maximum enzyme activity within a short cultivation period. To evaluate the residual concentration of emulsified PBSA in culture, emulsified PBSA in aliquots of culture supernatant was digested in vitro, and the concentration of released monomerised succinic acid was determined. Regardless of the initial concentration of emulsified PBSA in medium, PCLE activity was detected after residual succinic acid decreased below 0.04 mg/mL in culture broth. Jar fermentation was performed at a 0.27% PBSA concentration. Among the various airflow rates tested, 1 LPM resulted in a PCLE production rate of 1.0 U/mL/day. The enzyme activity in the resulting culture filtrate (4.2 U/2 mL) was shown to degrade commercial BP films (1 x 1 cm, 20 mu m thickness) within 8 hours.
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