Getting better all the time — recent progress in the development of CRISPR/Cas-based tools for plant genome engineering

Development of an efficient and precise adenine base editor (ABE) with expanded target range in allotetraploid cotton (Gossypium hirsutum)

(2022) Guanying Wang et al.   BMC BIOLOGY

PhieABEs: a PAM‐less/free high‐efficiency adenine base editor toolbox with wide target scope in plants

(2022) Jiantao Tan et al.   PLANT BIOTECHNOLOGY JOURNAL

An engineered prime editor with enhanced editing efficiency in plants

(2022) Yuan Zong et al.   NATURE BIOTECHNOLOGY

A cytosine base editor toolkit with varying activity windows and target scopes for versatile gene manipulation in plants

(2022) Xiangyu Xiong et al.   NUCLEIC ACIDS RESEARCH

Efficient C‐to‐G editing in rice using an optimized base editor

(2022) Yifu Tian et al.   PLANT BIOTECHNOLOGY JOURNAL

Genome‐wide analyses of PAM‐relaxed Cas9 genome editors reveal substantial off‐target effects by ABE8e in rice

(2022) Yuechao Wu et al.   PLANT BIOTECHNOLOGY JOURNAL

Heritable base-editing in Arabidopsis using RNA viral vectors

(2022) Degao Liu et al.   PLANT PHYSIOLOGY

CRISPR–Cas-mediated transcriptional control and epi-mutagenesis

(2022) Jason Gardiner et al.   PLANT PHYSIOLOGY

Using CRISPR-Kill for organ specific cell elimination by cleavage of tandem repeats

(2022) Angelina Schindele et al.   Nature Communications

Expanding the Editing Window of Cytidine Base Editors With the Rad51 DNA-Binding Domain in Rice

(2022) Chunjie Wei et al.   Frontiers in Plant Science

HDAC inhibitors improve CRISPR/Cas9 mediated prime editing and base editing

(2022) Nan Liu et al.   Molecular Therapy-Nucleic Acids

Wuschel2 enables highly efficient CRISPR/Cas-targeted genome editing during rapid de novo shoot regeneration in sorghum

(2022) Ping Che et al.   Communications Biology

Exploring C-to-G and A-to-Y Base Editing in Rice by Using New Vector Tools

(2022) Dongchang Zeng et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

An Intein-Mediated Split–nCas9 System for Base Editing in Plants

(2022) Guoliang Yuan et al.   ACS Synthetic Biology

A straightforward plant prime editing system enabled highly efficient precise editing of rice Waxy gene

(2022) Zhongsen Li et al.   PLANT SCIENCE

Enhancing gene editing and gene targeting efficiencies in Arabidopsis thaliana by using an intron‐containing version of tt Lb Cas12a

(2022) Patrick Schindele et al.   PLANT BIOTECHNOLOGY JOURNAL

Efficient gene targeting in Nicotiana tabacum using CRISPR/SaCas9 and temperature tolerant LbCas12a

(2021) Teng‐Kuei Huang et al.   PLANT BIOTECHNOLOGY JOURNAL

High-efficiency prime editing with optimized, paired pegRNAs in plants

(2021) Qiupeng Lin et al.   NATURE BIOTECHNOLOGY

The ScCas9 ++ variant expands the CRISPR toolbox for genome editing in plants

(2021) Taoli Liu et al.   Journal of Integrative Plant Biology

Adenine base editor engineering reduces editing of bystander cytosines

(2021) You Kyeong Jeong et al.   NATURE BIOTECHNOLOGY

Genome editing in mammalian cells using the CRISPR type I-D nuclease

(2021) Keishi Osakabe et al.   NUCLEIC ACIDS RESEARCH

Optimized nickase- and nuclease-based prime editing in human and mouse cells

(2021) Fatwa Adikusuma et al.   NUCLEIC ACIDS RESEARCH

Enhanced prime editing systems by manipulating cellular determinants of editing outcomes

(2021) Peter J. Chen et al.   CELL

Cas12a variants designed for lower genome-wide off-target effect through stringent PAM recognition

(2021) Jin Zhou et al.   MOLECULAR THERAPY

Programmable deletion, replacement, integration and inversion of large DNA sequences with twin prime editing

(2021) Andrew V. Anzalone et al.   NATURE BIOTECHNOLOGY

Genome editing with type II‐C CRISPR‐Cas9 systems from Neisseria meningitidis in rice

(2021) Rongfang Xu et al.   PLANT BIOTECHNOLOGY JOURNAL

Miniature type V-F CRISPR-Cas nucleases enable targeted DNA modification in cells

(2021) Greta Bigelyte et al.   Nature Communications

Targeted, random mutagenesis of plant genes with dual cytosine and adenine base editors

(2020) Chao Li et al.   NATURE BIOTECHNOLOGY

Targeted base editing in rice with CRISPR/ScCas9 system

(2020) Meixia Wang et al.   PLANT BIOTECHNOLOGY JOURNAL

Targeted mutagenesis in Arabidopsis thaliana using CRISPR‐Cas12b/C2c1

(2020) Fan Wu et al.   Journal of Integrative Plant Biology

Prime genome editing in rice and wheat

(2020) Qiupeng Lin et al.   NATURE BIOTECHNOLOGY

PAM recognition by miniature CRISPR–Cas12f nucleases triggers programmable double-stranded DNA target cleavage

(2020) Tautvydas Karvelis et al.   NUCLEIC ACIDS RESEARCH

A Cas9 with PAM recognition for adenine dinucleotides

(2020) Pranam Chatterjee et al.   Nature Communications

Rationally Designed APOBEC3B Cytosine Base Editors with Improved Specificity

(2020) Shuai Jin et al.   MOLECULAR CELL

Efficient Gene Targeting in Maize Using Inducible CRISPR-Cas9 and Marker-free Donor Template

(2020) Pierluigi Barone et al.   Molecular Plant

Changing local recombination patterns in Arabidopsis by CRISPR/Cas mediated chromosome engineering

(2020) Carla Schmidt et al.   Nature Communications

Genome editing mediated by SpCas9 variants with broad non-canonical PAM compatibility in plants

(2020) Juan Li et al.   Molecular Plant

CasX enzymes comprise a distinct family of RNA-guided genome editors

(2019) Jun-Jie Liu et al.   NATURE

Simplified adenine base editors improve adenine base editing efficiency in rice

(2019) Kai Hua et al.   PLANT BIOTECHNOLOGY JOURNAL

Expanding the scope of CRISPR/Cas9‐mediated genome editing in plants using an xCas9 and Cas9‐NG hybrid

(2019) Qingfeng Niu et al.   Journal of Integrative Plant Biology

Search-and-replace genome editing without double-strand breaks or donor DNA

(2019) Andrew V. Anzalone et al.   NATURE

Engineering CRISPR/ Lb Cas12a for highly efficient, temperature‐tolerant plant gene editing

(2019) Patrick Schindele et al.   PLANT BIOTECHNOLOGY JOURNAL

CRISPR/Cas brings plant biology and breeding into the fast lane

(2019) Angelina Schindele et al.   CURRENT OPINION IN BIOTECHNOLOGY