Article
Biochemistry & Molecular Biology
Anna Drohomirecka, Joanna Was, Natalia Wiligorska, Tomasz M. Rywik, Krzysztof Komuda, Dorota Sokolowska, Anna Lutynska, Tomasz Zielinski
Summary: Methylated arginine metabolites can disrupt nitric oxide synthesis, leading to endothelium dysfunction and inadequate vasodilation. This study aimed to investigate the dynamics of arginine derivatives during physical exercise in heart failure patients and assess its impact on patient outcomes.
Article
Biochemistry & Molecular Biology
Rocio Salsoso, Alfonso Mate, Fernando Toledo, Carmen M. Vazquez, Luis Sobrevia
Summary: Late-onset preeclampsia is associated with altered endothelial function in the fetoplacental vasculature, causing an imbalance in the L-arginine/NO signaling pathway and reducing umbilical vein dilation to insulin, which requires A(2B)AR activation in HUVECs.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2021)
Article
Medicine, Research & Experimental
Milene T. Fontes, Daniel F. Arruda-Junior, Danubia Silva dos Santos, Joao Carlos Ribeiro-Silva, Edinei L. Anto, Paulo F. J. Tucci, Luciana V. Rossoni, Adriana C. C. Girardi
Summary: This study tested the effects of DPP4 inhibitor vildagliptin on aortic hyperreactivity in male HF rats. The results showed that DPP4i mitigated the hypercontractility of HF aortas and enhanced NO bioavailability, suggesting a potential protective effect on the arteries.
Article
Biochemistry & Molecular Biology
Kira V. V. Derkach, Maxim A. A. Gureev, Anastasia A. A. Babushkina, Vladimir N. N. Mikhaylov, Irina O. O. Zakharova, Andrey A. A. Bakhtyukov, Viktor N. N. Sorokoumov, Alexander S. S. Novikov, Mikhail Krasavin, Alexander O. O. Shpakov, Irina A. A. Balova
Summary: This article describes the identification of dual inhibitors of protein phosphotyrosine phosphatase 1B (PTP1B)/T-cell protein phosphotyrosine phosphatase (TC-PTP) based on the 3-(hydroxymethyl)-4-oxo-1,4-dihydrocinnoline scaffold. The compounds were evaluated for their effects on obesity-related parameters and gene expressions. The results indicate the potential of these compounds as dual inhibitors for the treatment of metabolic disorders.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Multidisciplinary
Jinmin Miao, Jiajun Dong, Yiming Miao, Yunpeng Bai, Zihan Qu, Brenson A. Jassim, Bo Huang, Quyen Nguyen, Yuan Ma, Allison A. Murray, Jinyue Li, Philip S. Low, Zhong-Yin Zhang
Summary: In this study, researchers discovered the first highly potent and selective TC-PTP degrader called TP1L, which can induce degradation of TC-PTP and enhance T-cell signaling and tumor killing efficacy.
Review
Biochemistry & Molecular Biology
Maria Elisa Lopes-Pires, Jessica Oliveira Frade-Guanaes, Gregory J. Quinlan
Summary: Sepsis is a major cause of death among critically ill patients due to a host-mediated pro-inflammatory response to pathogen infection. Excessive oxidative/nitrosative stress and loss of vascular homeostatic control are common features of sepsis. Diagnosing and treating sepsis remains a significant challenge globally.
Article
Biotechnology & Applied Microbiology
Daquan Wang, Changyu Wang, Zhenxing Liang, Wangrui Lei, Chao Deng, Xiaoli Liu, Shuai Jiang, Yanli Zhu, Shaofei Zhang, Wenwen Yang, Ying Chen, Yao Qiu, Lingjie Meng, Yang Yang
Summary: This study found that nanoFe has a protective effect on septic myocardial injury by attenuating inflammation and oxidative stress, improving mitochondrial function, regulating endoplasmic reticulum stress, and activating the AMPK pathway. The RNA-seq results supported the role of nanoFe treatment in regulating a transcriptional profile consistent with its role in response to sepsis.
JOURNAL OF NANOBIOTECHNOLOGY
(2022)
Article
Pharmacology & Pharmacy
Jean-Louis Vincent, Can Ince, Peter Pickkers
Summary: This article discusses the different mechanisms underlying sepsis-related dysfunction of the vascular endothelium, including glycocalyx shedding, nitrosative stress, and coagulation factors, and mentions the potential therapeutic implications of each mechanism. While multiple targets to protect or restore endothelial function have been suggested, endothelium-driven treatments remain a future potential at present.
EXPERT OPINION ON THERAPEUTIC TARGETS
(2021)
Article
Biochemistry & Molecular Biology
Sonia Rocha, Mariana Lucas, Vera L. M. Silva, Pedro M. O. Gomes, Artur M. S. Silva, Alberto N. Araujo, Natalia Aniceto, Rita C. Guedes, M. Luisa Corvo, Eduarda Fernandes, Marisa Freitas
Summary: This study evaluated a library of 22 pyrazole compounds against human PTP1B activity, identifying two potent inhibitors and highlighting the importance of additional benzene rings in enhancing PTP1B inhibition. The most active compounds showed selectivity over TCPTP and molecular docking analyses revealed key residue contacts. This study represents a significant step in the design of novel PTP1B inhibitors.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Biochemistry & Molecular Biology
Bianca Maria Rotoli, Rossana Visigalli, Francesca Ferrari, Marianna Ranieri, Grazia Tamma, Valeria Dall'Asta, Amelia Barilli
Summary: This study evaluated the effects of desmopressin on human pulmonary microvascular endothelial cells and found that it had no cytotoxic effect on the endothelium, but significantly stimulated the production of nitric oxide.
Review
Physiology
Fulye Argunhan, Susan D. Brain
Summary: The treatment of hypertension and heart failure is a major challenge, with over 26 million people affected worldwide. CGRP has been found to have beneficial effects in hypertensive and heart failure patients. However, due to its short half-life, the beneficial effects of CGRP are often acute and transient. Recently developed long-lasting CGRP agonists may provide a solution for further studying CGRP in cardiovascular disease.
FRONTIERS IN PHYSIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Natasha de Alwis, Natalie K. Binder, Yeukai T. M. Mangwiro, Sally Beard, Natasha Pritchard, Elif Kadife, Bianca R. Fato, Emerson Keenan, Fiona C. Brownfoot, Tu'uhevaha J. Kaitu'u-Lino, Natalie J. Hannan
Summary: Esomeprazole, a proton pump inhibitor, shows potential therapeutic effects in the treatment of pregnancy-induced hypertension, and may work via the nitric oxide pathway. Further research is needed to fully understand its mechanism of action.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Pharmacology & Pharmacy
Samantha L. Saunders, Dana S. Hutchinson, Fiona C. Britton, Lu Liu, Irit Markus, Shaun L. Sandow, Timothy V. Murphy
Summary: The study demonstrated the presence of all three beta-adrenoceptor subtypes in the endothelium of rat cremaster muscle artery, with beta(3)-adrenoceptor mediated vasodilation only evident after blockade of beta(1/2)-adrenoceptors. Constitutive beta(1/2)-adrenoceptor activity inhibits beta(3)-adrenoceptor function in the endothelium of skeletal muscle resistance arteries.
BRITISH JOURNAL OF PHARMACOLOGY
(2021)
Article
Cardiac & Cardiovascular Systems
Ricardo Carnicer, Drew Duglan, Klemen Ziberna, Alice Recalde, Svetlana Reilly, Jillian N. Simon, Simona Mafrici, Ritu Arya, Esther Rosello-Lleti, Surawee Chuaiphichai, Damian Tyler, Craig A. Lygate, Keith M. Channon, Barbara Casadei
Summary: Myocardial BH4 was found to prevent and reverse left ventricular diastolic and systolic dysfunction associated with diabetes by increasing insulin-independent myocardial glucose uptake and utilization. This mechanism highlights the potential for GCH1/BH4-based therapeutics in human diabetic cardiomyopathy.
CIRCULATION RESEARCH
(2021)
Review
Medicine, General & Internal
Pasquale Ambrosino, Antimo Papa, Agostino Buonauro, Marco Mosella, Ilenia Calcaterra, Giorgio Alfredo Spedicato, Mauro Maniscalco, Matteo Nicola Dario Di Minno
Summary: Endothelial dysfunction is documented in patients with HFpEF, with a direct association between the severity of diastolic dysfunction and endothelial dysfunction. Strategies targeting endothelial dysfunction may provide a promising therapeutic option.
EUROPEAN JOURNAL OF CLINICAL INVESTIGATION
(2021)
Editorial Material
Gastroenterology & Hepatology
C. Lemaitre, P. -A. Thiebaut, A. Fracois, P. Michel
CLINICS AND RESEARCH IN HEPATOLOGY AND GASTROENTEROLOGY
(2021)
Article
Peripheral Vascular Disease
Jeremy Bellien, Michele Iacob, Vincent Richard, Julien Wils, Veronique Le Cam-Duchez, Robinson Joannides
Summary: Increasing wall shear stress in peripheral conduit arteries induces the release of t-PA, with high blood pressure potentially affecting this response and increasing the risk of arterial thrombosis.
HYPERTENSION RESEARCH
(2021)
Editorial Material
Pharmacology & Pharmacy
Mathieu Molimard, Vincent Richard, Jean-Luc Cracowski
Article
Cell Biology
Marion Bouvet, Emilie Dubois-Deruy, Annie Turkieh, Paul Mulder, Victoriane Peugnet, Maggy Chwastyniak, Olivia Beseme, Arthur Dechaumes, Philippe Amouyel, Vincent Richard, Nicolas Lamblin, Florence Pinet
Summary: This study identified an upregulation of active PKC zeta in heart failure rats, leading to phosphorylation and aggregation of desmin. Additionally, no modulation of the ubiquitin-proteasome system, a defect in macroautophagy, and an activation of chaperone-mediated autophagy were observed. These findings highlight a new signaling pathway associated with defective protein degradation systems in ischemic heart failure.
CELL DEATH DISCOVERY
(2021)
Article
Cell Biology
Philippe Drabent, Laura Polivka, Julie Agopian, Jean-Paul Duong Van Huyen, Pierre-Alain Thiebaut, Patrice Dubreuil, Olivier Hermine, Thierry Jo Molina, Sylvie Fraitag
Summary: The study analyzed 103 skin biopsies from patients with mastocytosis, determining the specific features of mast cell distribution and highlighting the percentage of mast cells as the most significant diagnostic criterion. Diagnosis of mastocytosis differed in difficulty between adults and children, with additional signs potentially useful in challenging cases.
Article
Pharmacology & Pharmacy
Regis Bordet, Jean-Christophe Dantonel, Eric Vacaresse, Claire Le Jeunne, Nora Benhabiles, Alain Beretz, Christian Boitard, Stephanie Debette, Gilles Duluc, Philippe Froguel, Benedicte Garbil, Stanislas Lyonnet, Abderrahim Mahfoudi, Pierre Marquet, Franck Mouthon, Olivier Rascol, Vincent Richard, Emmanuelle Simon, Nathalie Varoqueaux, Herve Watiert, Marie Zinsu
Summary: This article explores the impact of the Investments for the Future programme on health research and innovation, identifying five key factors for future development in healthcare innovation.
Article
Biochemistry & Molecular Biology
Thomas Duflot, Ly Tu, Matthieu Leuillier, Hind Messaoudi, Deborah Groussard, Guillaume Feugray, Saida Azhar, Raphael Thuillet, Fabrice Bauer, Marc Humbert, Vincent Richard, Christophe Guignabert, Jeremy Bellien
Summary: The study suggests that circulating lysophosphatidic acid may play a role in the pathophysiology of pulmonary hypertension. Further experiments are needed to assess the potential interest of modulating LPA signaling in pulmonary hypertension.
Article
Pharmacology & Pharmacy
Florian Lemaitre, Matthieu Gregoire, Caroline Monchaud, Stephane Bouchet, Beatrice Saint-Salvi, Elisabeth Polard, Sihem Benaboud, Laurent Chouchana, Jean-Luc Cracowski, Milou-Daniel Drici, Rodolphe Garraffo, Romain Guilhaumou, Annie-Pierre Jonville-Bera, Mathieu Molimard, Patric Muret, Gilles Peytavin, Vincent Richard, Caroline Solas
Summary: This study provides recommendations for the safe prescription of nirmatrelvir/ritonavir, aiming to prevent drug-drug interactions and ensure the efficacy of the treatment.
Article
Multidisciplinary Sciences
Matthieu Leuillier, Thomas Duflot, Severine Menoret, Hind Messaoudi, Zoubir Djerada, Deborah Groussard, Raphael G. P. Denis, Laurence Chevalier, Ahmed Karoui, Baptiste Panthu, Pierre-Alain Thiebaut, Isabelle Schmitz-Afonso, Severine Nobis, Cynthia Campart, Tiphaine Henry, Camille Sautreuil, Serge H. Luquet, Olivia Beseme, Catherine Feliu, Helene Peyret, Lionel Nicol, Jean-Paul Henry, Sylvanie Renet, Paul Mulder, Debin Wan, Laurent Tesson, Jean-Marie Heslan, Angeline Duche, Sebastien Jacques, Frederic Ziegler, Valery Brunel, Gilles J. P. Rautureau, Christelle Monteil, Jean-Luc do Rego, Jean-Claude do Rego, Carlos Afonso, Bruce Hammock, Anne-Marie Madec, Florence Pinet, Vincent Richard, Ignacio Anegon, Christophe Guignabert, Christophe Morisseau, Jeremy Bellien
Summary: The physiological role of the N-terminal phosphatase activity (sEH-P) of soluble epoxide hydrolase (sEH-H) was investigated using CRISPR/Cas9 to generate a knock-in (KI) rat line lacking sEH-P activity. The study found that sEH-P KI rats exhibited decreased metabolism of lysophosphatidic acids, decreased weight and fat mass gain, and increased insulin sensitivity. Moreover, sEH-P KI rats showed increased lipolysis and enhanced energy expenditure, which potentiated brown adipose thermogenesis. Additionally, sEH-P KI rats fed a high-fat diet did not experience weight gain, fat mass accumulation, insulin resistance, or hepatic steatosis, and they also exhibited enhanced cardiac mitochondrial activity and protection against ischemia-reperfusion injury. Overall, the findings highlight the importance of sEH-P in energy and fat metabolism and its potential therapeutic significance in the management of obesity and cardiac complications.
JOURNAL OF ADVANCED RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Victoriane Peugnet, Maggy Chwastyniak, Paul Mulder, Steve Lancel, Laurent Bultot, Natacha Fourny, Edith Renguet, Heiko Bugger, Olivia Beseme, Anne Loyens, Wilfried Heyse, Vincent Richard, Philippe Amouyel, Luc Bertrand, Florence Pinet, Emilie Dubois-Deruy
Summary: This study investigated the impact of mitochondria-targeted therapy on cardiac hypertrophy and found that MitoQ can reduce mitochondrial ROS and hypertrophy, but it may also affect mitochondrial structure and function by impairing mitochondrial respiration and mitophagy, particularly showing deleterious effects in cardiomyocytes.
Article
Cardiac & Cardiovascular Systems
Laurence Chevalier, Jean Selim, Celia Castro, Fabien Cuvilly, Jean-Marc Baste, Vincent Richard, Philippe Pareige, Jeremy Bellien
Summary: Glycocalyx is a layer of structure on the cell membrane composed of glycosaminoglycans and proteoglycans. It interacts with various proteins, contributing to vascular permeability and modulation of local inflammatory processes. This study developed innovative electron microscopy approaches to visualize the glycocalyx at the subcellular scale.
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2022)
Article
Peripheral Vascular Disease
Mouad Hamzaoui, Deborah Groussard, Dorian Nezam, Zoubir Djerada, Gaspard Lamy, Virginie Tardif, Anais Dumesnil, Sylvanie Renet, Valery Brunel, Dorien J. M. Peters, Laurence Chevalier, Melanie Hanoy, Paul Mulder, Vincent Richard, Jeremy Bellien, Dominique Guerrot
Summary: This study provides the first in vivo demonstration that specific deletion of Pkd1 in endothelial cells promotes endothelial dysfunction and hypertension, impairs arteriovenous fistula development, and potentiates the cardiovascular alterations associated with chronic kidney disease.
Article
Biochemistry & Molecular Biology
Jean Selim, Mouad Hamzaoui, Antoine Ghemired, Zoubir Djerada, Laurence Chevalier, Nicolas Piton, Emmanuel Besnier, Thomas Clavier, Anais Dumesnil, Sylvanie Renet, Paul Mulder, Fabien Doguet, Fabienne Tamion, Benoit Veber, Jeremy Bellien, Vincent Richard, Jean-Marc Baste
Summary: The use of human albumin and hypertonic sodium lactate as priming agents during mechanical circulatory support in lung transplantation can reduce pulmonary endothelial dysfunction. However, hypertonic sodium lactate may lead to the development of pulmonary edema.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Editorial Material
Pharmacology & Pharmacy
Jean-Luc Cracowski, Vincent Richard, Mathieu Molimard
Article
Surgery
Mickael Palmier, Emlyn Cornet, Sylvanie Renet, Anais Dumesnil, Nicolas Perzo, Quentin Cohen, Vincent Richard, Didier Plissonnier
Summary: This study investigated the lung injury induced by supraceliac aortic cross clamping through an inflammatory ischemia-reperfusion trigger and analyzed the role of glycocalyx (GCX), a component of the endothelial membrane, in remote lung injury. The results showed that plasma levels of GCX products (Sdc-1 and HS), TNF-α, and IL-10 reached peak levels at different time points during reperfusion. The levels were significantly higher in the clamping groups compared to the sham group. Lung levels of TNF-α and IL-10 also reached peak levels at 6 hours, and were significantly higher than the sham group. Histopathologic scores and pulmonary gravimetry showed more severe lung injury and pulmonary edema in the clamping groups compared to the sham group at different time points. In conclusion, supraceliac aortic clamping causes early lung injury associated with a systemic inflammatory response and altered GCX structure.
ANNALS OF VASCULAR SURGERY
(2023)
Article
Cardiac & Cardiovascular Systems
Andrew B. Harvey, Renelyn A. Woltes, Raymond N. Deepe, Hannah G. Tarolli, Jenna R. Drummond, Allison Trouten, Auva Zandi, Jeremy L. Barth, Rupak Mukherjee, Martin J. Romeo, Silvia G. Vaena, Ge Tao, Robin Muise-Helmericks, Paula S. Ramos, Russell A. Norris, Andy Wessels
Summary: This study highlights the importance of SOX9 in the regulation of epicardial cell invasion and emphasizes the role of EPDCs in regulating atrioventricular valve development and homeostasis. It also reports a novel expression profile of Cd109, a gene with previously unknown relevance in heart development.
JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY
(2024)
Review
Cardiac & Cardiovascular Systems
MariaSanta C. Mangione, Jinhua Wen, Dian J. Cao
Summary: mTOR, a mechanistic target of rapamycin, is an evolutionarily conserved pathway that plays a fundamental role in nutrient sensing, growth, metabolism, lifespan, and aging. Recent studies have highlighted the regulatory role of mTOR in innate immune responses and its involvement in the pathogenesis of cardiovascular diseases, especially in acute inflammation and atherosclerotic cardiovascular disease. This review also discusses mTOR's role in trained immunity, immune senescence, and clonal hematopoiesis, as well as its architecture and regulatory complexes.
JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY
(2024)
Article
Cardiac & Cardiovascular Systems
Junlin Li, Yajun Gong, Yiren Wang, Huihui Huang, Huan Du, Lianying Cheng, Cui Ma, Yongxiang Cai, Hukui Han, Jianhong Tao, Gang Li, Panke Cheng
Summary: Myocardial ischemia-reperfusion injury is closely related to the final infarct size in acute myocardial infarction. Regulatory T cells play an important role in the inflammatory response after AMI, but different subtypes of Tregs have different effects on the injury.
JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY
(2024)
Article
Cardiac & Cardiovascular Systems
Yuxin Chu, Yutao Hua, Lihao He, Jin He, Yunxi Chen, Jing Yang, Ismail Mahmoud, Fanfang Zeng, Xiaochang Zeng, Gloria A. Benavides, Victor M. Darley-Usmar, Martin E. Young, Scott W. Ballinger, Sumanth D. Prabhu, Cheng Zhang, Min Xie
Summary: This study demonstrates that administering beta-hydroxybutyrate (beta-OHB) at the time of reperfusion can reduce infarct size and preserve cardiac function by activating autophagy and preserving mitochondrial homeostasis, potentially through mTOR inhibition.
JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY
(2024)