Simultaneous Detection of Ochratoxin A and Aflatoxins in Industrial and Traditional Red and isot Pepper Flakes along with Dietary Exposure Risk Assessment

This study focused on the co-occurrence of aflatoxins (AFs) and ochratoxin A (OTA) in traditionally and industrially dried red pepper flakes (DRPFs) and isot pepper flakes (IPFs). Following the multitoxin immunoaffinity column (IAC) clean-up, high-performance liquid chromatography coupled with fluorescence detection (HPLC-FLD) was used to quantify AFs and OTA. The limit of detection (LOD) and limit of quantification (LOQ) values were 0.11 and 0.18 mu g kg(-1) (AFB1), 0.04 and 0.08 mu g kg(-1) (AFB(2)), 0.13 and 0.18 mu g kg(-1) (AFG(1)), 0.04 and 0.11 mu g kg(-1) (AFG(2)), and 0.10 and 0.21 mu g kg(-1) (OTA), respectively. AFB(1), AFB(2), AFG(1), and OTA were found to be positive in 93, 74, 17, and 94% of all samples, respectively. The contamination levels in positive samples ranged from 0.23 to 38.69, 0.04 to 2.14, 0.13 to 0.88, and 0.18 to 52.19 mu g kg(-1) for AFB(1), AFB(2), AFG(1), and OTA, respectively, while no AFG(2) was found above the detection limit (0.04 mu g kg(-1)). None of the industrial isot peppers exceeded the European Union limits, while the levels of AFB(1) (5 mu g kg(-1)), total AFs (10 mu g kg(-1)), and OTA (20 mu g kg(-1)) of the traditional peppers were above the limit by 30% (16/54), 26% (14/54), and 4% (2/54), respectively. Co-occurrence of AFB(1)-AFB(2)-OTA was the most frequent, accounting for 54% (29/54) of all samples. At the upper bound (UB), estimated average exposure to AFB(1), total AFs, and OTA was determined to be 0.175, 0.189, and 0.124 ng kg(-1) bw day(-1) in all samples, respectively. The margin of exposure (MoE) value of AFB(1) and total AFs was found to be 977 and 909, indicating high health concerns compared to OTA (MoE >10,000). AFB(1) and total AFs may result in 0.0058 and 0.0062 liver cancer cases/100,000 person/year at UB, respectively, and weekly OTA exposure was 0.868 ng kg(-1) bw, well below the provisional tolerable weekly intake, hence not of health concern. AFs exposure could endanger health, whereas OTA posed no toxicological concerns through dried red pepper consumption

Automated summary

This study investigated the occurrence of aflatoxins (AFs) and ochratoxin A (OTA) in dried red pepper flakes. The results showed that AFB1, AFB2, AFG1, and OTA were detected in the samples, with AFB1 and total AFs exceeding the European Union limits in traditional peppers. The exposure to AFB1 and total AFs may pose health risks, while OTA exposure was below health concern levels.