Review
Oncology
Ye Gu, Xiaofeng Zhang, Weiping Yu, Weifeng Dong
Summary: This article reviews the methylation function of SET7/9 and discusses its important impact mechanisms on cancer initiation and development. The article also explores the function of SET7/9 in regulating the expression and stability of protein targets and discusses its potential role as an oncogene or tumor suppressor in different cancer types.
Article
Biochemistry & Molecular Biology
Dmitry Myadelets, Sergey Parfenyev, Julia Vasileva, Oleg Shuvalov, Alexey Petukhov, Olga Fedorova, Nickolai Barlev, Alexandra Daks
Summary: The protein-specific methyltransferase Set7/9 plays a crucial role in various cellular processes, including histone modification and transcription factor regulation. It has been linked to tumor growth and the transformation of normal cells. The impact of Set7/9 on cancer progression is complex and dependent on the type of cancer. This study reveals the potential involvement of Set7/9 in DNA damage signaling and repair processes in breast cancer cells. The downregulation of Set7/9 is associated with decreased expression of PARP1 and increased resistance to cisplatin. The combination of cisplatin and the FDA approved PARP1 inhibitor niraparib shows promising synergistic effects in overcoming cisplatin resistance in Set7/9 deficient breast cancer cells.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biology
Alexandra Daks, Elena Vasileva, Olga Fedorova, Oleg Shuvalov, Nickolai A. Barlev
Summary: In this review, we analyze the particularities of SET7/9 enzymatic activity and substrate specificity with respect to its biological importance, mostly focusing on its two well-characterized biological functions: cellular proliferation and stress response.
Review
Oncology
Lili Gao, Weiping Yu, Peng Song, Qing Li
Summary: SET7/9 is an important lysine methyltransferase involved in regulating various biological processes, playing a key role in tumorigenesis and development.
RECENT PATENTS ON ANTI-CANCER DRUG DISCOVERY
(2022)
Article
Biochemistry & Molecular Biology
Himanshu Sankrityayan, Ajinath Kale, Vishwadeep Shelke, Anil Bhanudas Gaikwad
Summary: The study demonstrated that the novel SET7/9 inhibitor, Cyproheptadine, has renoprotective potential by reducing H3K4Me1 expression and ER stress to prevent diabetic kidney disease.
ARCHIVES OF PHYSIOLOGY AND BIOCHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Alexandra Daks, Victoria Mamontova, Olga Fedorova, Alexey Petukhov, Oleg Shuvalov, Sergey Parfenyev, Sofia Netsvetay, Aigul Venina, Alena Kizenko, Evgeny Imyanitov, Nickolai Barlev
Summary: Set7/9 regulates cell proliferation, cancer progression, and DNA damage response by post-translationally modifying critical transcription factors.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Review
Cell Biology
Shangzhen Yang, Xi Wang, Jun Bai, Baojun Duan
Summary: SET domain containing lysine methyltransferase 7 (SETD7) plays a vital role in the regulation of cell cycle, cell differentiation, DNA damage response, and chromatin remodeling through histone methylation. Abnormal expression of SETD7 is associated with tumor development, promoting tumor cell proliferation and invasion, and predicting poor prognosis. Understanding the role of SETD7 in tumor development is important for exploring its regulatory mechanisms and potential targeted therapies.
Article
Chemistry, Medicinal
Alessandra Feoli, Giulia Iannelli, Alessandra Cipriano, Ciro Milite, Lei Shen, Zhihao Wang, Andrea Hadjikyriacou, Troy L. Lowe, Cyrus Safaeipour, Monica Viviano, Giuliana Sarno, Elva Morretta, Maria Chiara Monti, Yanzhong Yang, Steven G. Clarke, Sandro Cosconati, Sabrina Castellano, Gianluca Sbardella
Summary: PRMT7 and PRMT9 have been identified as important therapeutic targets, but their biological roles, functions, and structural requirements are still unclear. This study discovered a potent PRMT7/9 inhibitor and revealed its binding mode to the two enzymes. The inhibition of PRMT activity in cells was confirmed, and an effective AlphaLISA assay was developed for screening small molecule inhibitors of PRMT9.
JOURNAL OF MEDICINAL CHEMISTRY
(2023)
Article
Multidisciplinary Sciences
Liping Liu, Mengnan Chai, Youmei Huang, Jingang Qi, Wenhui Zhu, Xinpeng Xi, Fangqian Chen, Yuan Qin, Hanyang Cai
Summary: Inflorescence architecture is regulated by the inflorescence meristem and pedicel length. SDG2 genetically interacts with the SWR1-ER signaling pathways to influence inflorescence growth, potentially mediated by auxin. SWR1 and ER signaling are necessary for H2A.Z histone variant enrichment, while SDG2 regulates SDG2-mediated H3K4me3 histone modification at auxin-related genes and H2A.Z histone variant enrichment, affecting auxin hormone signaling pathways.
Article
Multidisciplinary Sciences
Rhian Jones, Susanne Wijesinghe, Claire Wilson, John Halsall, Triantafillos Liloglou, Aditi Kanhere
Summary: The nuclear lincRNA CCDC26 plays a crucial role in regulating DNMT1 activity and controlling DNA methylation levels. Its absence leads to mis-localization of DNMT1 to the cytoplasm, causing significant hypomethylation of genomic DNA, double-stranded DNA breaks, and increased cell death. This study reveals a previously unrecognized mechanism of lincRNA-mediated subcellular localization of DNMT1 and regulation of DNA methylation.
Article
Biochemistry & Molecular Biology
Hyunsoo Kim, Noriko Takegahara, Yongwon Choi
Summary: Pcdh7 regulates osteoclast differentiation by controlling the activation of RhoA and Rac1 through its SET binding domain. Restoring full-length Pcdh7 through retroviral transduction can rescue impaired osteoclast differentiation in Pcdh7-deficient cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Fatemeh Mirzadeh Azad, Eduard A. Struys, Victoria Wingert, Luciana Hannibal, Ken Mills, Joop H. Jansen, Daniel B. Longley, Hendrik G. Stunnenberg, Yaser Atlasi
Summary: Understanding the mechanisms of epigenetic regulation in ESCs is crucial for stem cell and developmental biology. In this study, the identification of Spic as a marker of ground state pluripotency and its role in controlling 1C metabolism and histone marks highlight the importance of auxiliary TFs in linking cellular metabolism to epigenetic regulation in ESCs.
Article
Oncology
Xiaolin Sun, Xiang Gu, Hongxiao Li, Pei Xu, Mengting Li, Ye Zhu, Qisheng Zuo, Bichun Li
Summary: Studies have shown that the epigenetic modifier H3K9me2 plays an important role in embryonic development, cell reprogramming, and cell differentiation. This study demonstrated that H3K9me2 is a negative regulator of cardiomyocyte formation by influencing the expression of key transcription factors, providing insights for optimizing the induction efficiency of cardiomyocytes in vitro and in clinical applications.
MOLECULAR MEDICINE REPORTS
(2021)
Article
Biology
Pierre-Olivier Esteve, Sagnik Sen, Udayakumar S. Vishnu, Cristian Ruse, Hang Gyeong Chin, Sriharsa Pradhan
Summary: SET8, the enzyme responsible for H4K20me1, is post-translationally poly ADP-ribosylated by PARP1, leading to its degradation and aberrant H4K20 methylation. This regulation plays important roles in mitotic condensation, DNA replication, DNA damage response, and gene expression.
COMMUNICATIONS BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Liane P. Fernandes, Rocio Enriquez-Gasca, Poppy A. Gould, James H. Holt, Lucia Conde, Gabriela Ecco, Javier Herrero, Robert Gifford, Didier Trono, George Kassiotis, Helen M. Rowe
Summary: Research has found that the regulation of cell fate can be influenced by ZFP819, which targets a satellite DNA array called ZP3AR. The depletion of ZFP819 leads to a transition from a pluripotent state to a 2-cell-like state in cells. This transition is accompanied by changes in chromatin structure and the inactivation of master transcription factor genes.
Article
Biochemistry & Molecular Biology
K. Dhanalakshmi, Seiki Kuramitsu, Shigeyuki Yokoyama, Kumarevel Thirumananseri, Karthe Ponnuraj
Summary: This study reports the crystal structure of arginase from Thermus thermophilus (TtArginase), and compares it with other homologous structures. The results show that TtArginase is more stable compared to its mesophilic counterpart, Bacillus subtilis arginase (BsArginase). Additionally, the study reveals the critical role of metal ions in both the catalytic function and maintenance of proper active site geometry. These findings are important for optimizing the enzymatic action of arginase in various conditions.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2023)
Article
Biochemistry & Molecular Biology
K. Dhanalakshmi, Seiki Kuramitsu, Shigeyuki Yokoyama, Thirumananseri Kumarevel, Karthe Ponnuraj
Summary: The crystal structure of pyrrolidone carboxyl peptidase from Thermus thermophilus (TtPCP) was solved and refined at 1.9 angstrom resolution using the molecular replacement method. Comparing TtPCP with its structural homologs, it was found that the putative thermal stability of TtPCP may be attributed to more intra and inter-molecular hydrogen bonds, hydrophobic and ion pair interactions. This structural information of TtPCP can contribute to understanding the intrinsic stability of thermophilic proteins and can be useful for protein engineering.
BIOPHYSICAL CHEMISTRY
(2023)
Editorial Material
Biochemistry & Molecular Biology
Minoru Yoshida, Seung Bum Park
CURRENT OPINION IN CHEMICAL BIOLOGY
(2023)
Editorial Material
Biotechnology & Applied Microbiology
Richard E. Lee, Minoru Yoshida
JOURNAL OF ANTIBIOTICS
(2023)
Article
Biochemistry & Molecular Biology
Hideaki Ohtomo, Shinsuke Ito, Nicholas J. McKenzie, Michael Uckelmann, Masatoshi Wakamori, Haruhiko Ehara, Ayako Furukawa, Yasuo Tsunaka, Marika Shibata, Shun-ichi Sekine, Takashi Umehara, Chen Davidovich, Haruhiko Koseki, Yoshifum Nishimura
Summary: PRC1 and PRC2 play important roles in epigenetic gene regulation. H2A ubiquitination by PRC1 promotes H3K27 methylation by PRC2 through altering the dynamics of H3-tail and its contacts with DNA, with the linker-DNA being crucial for this process.
JOURNAL OF MOLECULAR BIOLOGY
(2023)
Article
Multidisciplinary Sciences
Shohei Takase, Takashi Hiroyama, Fumiyuki Shirai, Yuki Maemoto, Akiko Nakata, Mayumi Arata, Seiji Matsuoka, Takeshi Sonoda, Hideaki Niwa, Shin Sato, Takashi Umehara, Mikako Shirouzu, Yosuke Nishigaya, Tatsunobu Sumiya, Noriaki Hashimoto, Ryosuke Namie, Masaya Usui, Tomokazu Ohishi, Shun-ichi Ohba, Manabu Kawada, Yoshihiro Hayashi, Hironori Harada, Tokio Yamaguchi, Yoichi Shinkai, Yukio Nakamura, Minoru Yoshida, Akihiro Ito
Summary: Sickle cell disease (SCD) is caused by beta-globin gene mutations. G9a inhibitors, including RK-701, have been proposed as therapeutic agents for inducing fetal globin expression. This study describes the development of RK-701 as a selective and non-genotoxic G9a inhibitor, which upregulates BGLT3 long non-coding RNA and induces gamma-globin expression. BGLT3 is identified as an essential factor for gamma-globin induction and its universal role suggests its importance in SCD treatment.
NATURE COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Mutsuko Kukimoto-Niino, Kentaro Ihara, Chiemi Mishima-Tsumagari, Mio Inoue, Yoshinori Fukui, Shigeyuki Yokoyama, Mikako Shirouzu
Summary: This study reveals the crystal structures of the catalytic DHR2 domain of mouse DOCK10, complexed with either Cdc42 or Rac1, and uncovers the unique dual activation mechanism of DOCK10.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Jennifer Nhieu, Liming Milbauer, Thomas Lerdall, Fatimah Najjar, Chin-Wen Wei, Ryosuke Ishida, Yue Ma, Hiroyuki Kagechika, Li-Na Wei
Summary: All-trans-retinoic Acid (atRA), derived from Vitamin A, plays crucial roles in various biological processes. It can modulate gene expression through nuclear RA receptors (RARs) or regulate cytoplasmic kinase signaling via cellular retinoic acid binding protein 1 (CRABP1). This study focuses on identifying CRABP1-binding ligands without RAR activity for potential therapeutic applications. The researchers developed a P19-MN differentiation system to investigate the effects of CRABP1 ligands in motor neuron (MN) differentiation and discovered C32 and C4 as CRABP1 ligands that can modulate CaMKII activation and protect against excitotoxicity-triggered MN death. These findings suggest the potential of selective and CRABP1-binding ligands in mitigating MN degenerative diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Tatsuo Yanagisawa, Eiko Seki, Hiroaki Tanabe, Yoshifumi Fujii, Kensaku Sakamoto, Shigeyuki Yokoyama
Summary: Pairs of pyrrolysyl-tRNA synthetase (PylRS) and tRNA(Pyl) from Methanosarcina mazei and Methanosarcina barkeri are used for site-specific incorporations of non-canonical amino acids into proteins. Mutations in Methanomethylophilus alvus PylRS allow efficient binding with N-e-((((E)-cyclooct-2-en-1-yl)oxy)carbonyl)-L-lysine (TCO*Lys) but not with N-e-(p-ethynylbenzyloxycarbonyl)-L-lysine (pEtZLys).
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell Biology
Kota Noritsugu, Takehiro Suzuki, Kosuke Dodo, Kenji Ohgane, Yasue Ichikawa, Kota Koike, Satoshi Morita, Takashi Umehara, Kenji Ogawa, Mikiko Sodeoka, Naoshi Dohmae, Minoru Yoshida, Akihiro Ito
Summary: TEAD transcription factors regulate the transcriptional output of Hippo signaling by phosphorylating YAP and TAZ as coactivators. Recent studies have shown that cysteine palmitoylation also plays a role in regulating TEAD activity. This study discovers lysine long-chain fatty acylation as another post-translational modification of TEADs. Lysine fatty acylation enhances the interaction between TEADs and YAP/TAZ, contributing to their transcriptional activity. Interestingly, lysine fatty acylation of TEADs increases upon Hippo signaling activation, despite a decrease in cysteine acylation. These findings provide new insights into the regulation of TEAD activity through fatty-acyl modifications.
Article
Biochemistry & Molecular Biology
Israa Mohammad Al-Amily, Marie Sjogren, Pontus Duner, Mohammad Tariq, Claes. B. B. Wollheim, Albert Salehi
Summary: The activation of GPR56 by Coll III promotes cell growth, proliferation, and survival. The signaling cascades mediating this effect involve VDAC1 expression in pancreatic beta-cells. Inhibition of GPR56 attenuates the suppressive effect of Coll III on various phosphorylation pathways and increases the expression of Chrebp, Txnip, and Vdac1. The translocation of Vdac1 to the plasma membrane in GPR56-KD cells leads to ATP loss, reduced cAMP production, and impaired glucose-stimulated insulin secretion.
Article
Multidisciplinary Sciences
Masaki Kikuchi, Satoshi Morita, Masatoshi Wakamori, Shin Sato, Tomomi Uchikubo-Kamo, Takehiro Suzuki, Naoshi Dohmae, Mikako Shirouzu, Takashi Umehara
Summary: In this study, the authors discovered that p300/CBP recognizes and acetylates histone H4 N-terminal tail (NT) acetylation (ac) in a nucleosome, and also acetylates non-H4 histone NTs within the same nucleosome. The authors propose a model in which p300/CBP replicates histone N-terminal tail acetylation within the H3-H4 tetramer and transfers it to the H2B-H2A dimers to activate context-dependent gene transcription through local nucleosome destabilization.
NATURE COMMUNICATIONS
(2023)
Article
Chemistry, Multidisciplinary
Liliana C. Fernandes, Daniela M. Correia, Mohammad Tariq, Jose M. S. S. Esperanca, Pedro Martins, Senentxu Lanceros-Mendez
Summary: With the increase in demand for miniaturized multifunctional devices, especially sensors and actuators, the development of smart materials with magnetoelectric (ME) properties has become important. The study demonstrates the suitability of magnetoelectric responsive materials for magnetic sensing and actuation devices. The materials show voltage responses in the mV range, making them suitable for high sensitivity magnetic field sensors.
Article
Immunology
Seisuke Kusano, Sho Ueda, Daisuke Oryoji, Aya Toyoumi, Akiko Hashimoto-Tane, Hiroyuki Kishi, Hiroshi Hamana, Atsushi Muraguchi, Hui Jin, Hisashi Arase, Hiroko Miyadera, Reiko Kishikawa, Yasunobu Yoshikai, Hisakata Yamada, Ken Yamamoto, Yasuharu Nishimura, Takashi Saito, Takehiko Sasazuki, Shigeyuki Yokoyama
Summary: Cry j 1 is a major allergen in Japanese cedar pollens. The NF region of Cry j 1 peptide plays a role in enhancing T-cell activation. Mutation of Ser and Lys in the NF region to Glu reduces the affinity for HLA-DP5, antigen presentation, and T-cell activation.
INTERNATIONAL IMMUNOLOGY
(2023)