Journal
NANOMATERIALS
Volume 12, Issue 21, Pages -Publisher
MDPI
DOI: 10.3390/nano12213854
Keywords
SARS-CoV-2; COVID-19; SERS; lithography; aptamer; biosensor
Categories
Funding
- Russian Science Foundation [19-72-30003]
Ask authors/readers for more resources
This technology utilizes double-labeled DNA aptamers and the SERS method for rapid and sensitive detection of SARS-CoV-2, featuring high speed, specificity, and ultrasensitive quantification capabilities, comparable to the conventional reverse transcription polymerase chain reaction method.
In this paper, we propose a technology for the rapid and sensitive detection of the whole viral particles of SARS-CoV-2 using double-labeled DNA aptamers as recognition elements together with the SERS method for detecting the optical response. We report on the development of a SERS-aptasensor based on a reproducible lithographic SERS substrate, featuring the combination of high speed, specificity, and ultrasensitive quantitative detection of SARS-CoV-2 virions. The sensor makes it possible to identify SARS-CoV-2 in very low concentrations (the limit of detection was 100 copies/mL), demonstrating a sensitivity level comparable to the existing diagnostic golden standard-the reverse transcription polymerase chain reaction.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available