M-TUBE enables large-volume bacterial gene delivery using a high-throughput microfluidic electroporation platform

Functional genetics of human gut commensal Bacteroides thetaiotaomicron reveals metabolic requirements for growth across environments

(2021) Hualan Liu et al.   Cell Reports

A nonviral, nonintegrating DNA nanovector platform for the safe, rapid, and persistent manufacture of recombinant T cells

(2021) Matthias Bozza et al.   Science Advances

Rapid ordering of barcoded transposon insertion libraries of anaerobic bacteria

(2021) Anthony L. Shiver et al.   Nature Protocols

Publisher Correction: Calibration of CAR activation potential directs alternative T cell fates and therapeutic potency

(2019) Judith Feucht et al.   NATURE MEDICINE

CAR T cell trogocytosis and cooperative killing regulate tumour antigen escape

(2019) Mohamad Hamieh et al.   NATURE

Engineered E. coli Nissle 1917 for the delivery of matrix-tethered therapeutic domains to the gut

(2019) Pichet Praveschotinunt et al.   Nature Communications

Improvement of electroporation-mediated transformation efficiency for a Bifidobacterium strain to a reproducibly high level

(2018) Min Ju Park et al.   JOURNAL OF MICROBIOLOGICAL METHODS

High efficiency hydrodynamic bacterial electrotransformation

(2017) Paulo A. Garcia et al.   LAB ON A CHIP

Targeting a CAR to the TRAC locus with CRISPR/Cas9 enhances tumour rejection

(2017) Justin Eyquem et al.   NATURE

Scalable Device for Automated Microbial Electroporation in a Digital Microfluidic Platform

(2017) Andrew C. Madison et al.   ACS Synthetic Biology

The essential genomic landscape of the commensal Bifidobacterium breve UCC2003

(2017) Lorena Ruiz et al.   Scientific Reports

CRISPR/Cas9 β-globin gene targeting in human haematopoietic stem cells

(2016) Daniel P. Dever et al.   NATURE

Directed evolution using dCas9-targeted somatic hypermutation in mammalian cells

(2016) Gaelen T Hess et al.   NATURE METHODS

Impact of external medium conductivity on cell membrane electropermeabilization by microsecond and nanosecond electric pulses

(2016) Aude Silve et al.   Scientific Reports

Pangenome analysis of Bifidobacterium longum and site-directed mutagenesis through by-pass of restriction-modification systems

(2015) A. O’Callaghan et al.   BMC GENOMICS

Electroporation-based applications in biotechnology

(2015) Tadej Kotnik et al.   TRENDS IN BIOTECHNOLOGY

Rapid Quantification of Mutant Fitness in Diverse Bacteria by Sequencing Randomly Bar-Coded Transposons

(2015) Kelly M. Wetmore et al.   mBio

Probiotic Bifidobacterium longum alters gut luminal metabolism through modification of the gut microbial community

(2015) Hirosuke Sugahara et al.   Scientific Reports

Physical methods for genetic transformation of fungi and yeast

(2014) Ana Leonor Rivera et al.   Physics of Life Reviews

Joule heating effects on particle immobilization in insulator-based dielectrophoretic devices

(2013) Roberto C. Gallo-Villanueva et al.   ELECTROPHORESIS

Thermal loading in flow-through electroporation microfluidic devices

(2013) Blanca del Rosal et al.   LAB ON A CHIP

Microfluidic electroporation for cellular analysis and delivery

(2013) Tao Geng et al.   LAB ON A CHIP

Transposon Mutagenesis in Bifidobacterium breve: Construction and Characterization of a Tn5 Transposon Mutant Library for Bifidobacterium breve UCC2003

(2013) Lorena Ruiz et al.   PLoS One

Low-frequency ac electroporation shows strong frequency dependence and yields comparable transfection results to dc electroporation

(2012) Yihong Zhan et al.   JOURNAL OF CONTROLLED RELEASE

Transfection of cells using flow-through electroporation based on constant voltage

(2011) Tao Geng et al.   Nature Protocols

Intracranial Nonthermal Irreversible Electroporation: In Vivo Analysis

(2010) Paulo A. Garcia et al.   JOURNAL OF MEMBRANE BIOLOGY

Microfluidics cell electroporation

(2010) Saeid Movahed et al.   Microfluidics and Nanofluidics

Plasmid uptake by bacteria: a comparison of methods and efficiencies

(2009) Naoto Yoshida et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Micro-electroporation of mesenchymal stem cells with alternating electrical current pulses

(2008) Roee Ziv et al.   BIOMEDICAL MICRODEVICES

Microfluidic electroporation for delivery of small molecules and genes into cells using a common DC power supply

(2008) Hsiang-Yu Wang et al.   BIOTECHNOLOGY AND BIOENGINEERING

Suspended-drop electroporation for high-throughput delivery of biomolecules into cells

(2008) Emmanuel G Guignet et al.   NATURE METHODS