Article
Multidisciplinary Sciences
Phan Tan Toi, Hyun Jae Jang, Kyeongseon Min, Sung-Phil Kim, Seung-Kyun Lee, Jongho Lee, Jeehyun Kwag, Jang-Yeon Park
Summary: Researchers have developed a two-dimensional fast line-scan approach that allows direct imaging of neuronal activity with millisecond precision, while maintaining high spatial resolution. This method was demonstrated in live mouse brain imaging, showing the sequential and laminar-specific propagation of neuronal activity along the thalamocortical pathway.
Article
Optics
Zhenfei Jiao, Zhou Zhou, Zhongyun Chen, Jinze Xie, Yu Mu, Jiulin DU, Ling Fu
Summary: To understand the functional neural circuits, it is crucial to acquire the firing sequence of neurons at different depths with cellular resolution. The Simple Multi-Plane Imaging Light-Sheet Fluorescence Microscopy (SIMPLE-LSFM) combines multi-plane illumination and spherical-aberration-assisted point spread function to simultaneously image neurons at different depths. This method was successfully demonstrated in capturing the firing sequences of neurons at six depths in larval zebrafish, providing fast and high-resolution mapping of anatomical structure and dynamics.
Article
Optics
KyeoReh Lee, Jun Lim, Su Yong Lee, YongKeun Park
Summary: Coherent speckle-correlation imaging (CSI) utilizes a designed X-ray diffuser to retrieve the complex sample field from a single shot measurement, achieving high-resolution imaging without any assumptions on the samples or measurements. The spatial resolution of CSI reaches 13.9 nm at 5.46 keV, surpassing the feature size of the diffuser used (300 nm). The high-resolution imaging capability of CSI is theoretically explained, and it is expected to be a versatile tool for exploring the nanometer world.
LIGHT-SCIENCE & APPLICATIONS
(2023)
Article
Chemistry, Multidisciplinary
Ali Yasin Sonay, Konstantinos Kalyviotis, Sine Yaganoglu, Aysen Unsal, Martina Konantz, Claire Teulon, Ingo Lieberwirth, Sandro Sieber, Shuai Jiang, Shahed Behzadi, Daniel Crespy, Katharina Landfester, Sylvie Roke, Claudia Lengerke, Periklis Pantazis
Summary: Optical imaging probes are important in disease detection, and biodegradable harmonophores offer a new approach for cancer treatment through targeted high-resolution optical imaging.
Article
Multidisciplinary Sciences
Antoine Labeyrie
Summary: The improved 'hypertelescope' version has been proposed for use on Earth, in space, and now on the Moon, allowing for direct high-resolution imaging with a high limiting magnitude. By utilizing many small mirrors arrayed in a lunar impact crater, a wide range of science targets can be studied, including near-Earth objects, exoplanets, neutron stars and black holes, and distant galaxies. Future larger versions, potentially spanning thousands of kilometers in space, could provide even higher resolution imaging capabilities for a variety of astronomical research purposes.
PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY A-MATHEMATICAL PHYSICAL AND ENGINEERING SCIENCES
(2021)
Article
Multidisciplinary Sciences
Wei Chen, Ryan G. Natan, Yuhan Yang, Shih-Wei Chou, Qinrong Zhang, Ehud Y. Isacoff, Na Ji
Summary: Studying neuronal activity at synapses requires high spatiotemporal resolution, which can be achieved through adaptive optics to correct sample-induced aberrations at depth for high spatial resolution. The combination of Bessel focus with two-photon fluorescence microscopy allows for fast volumetric imaging at subcellular lateral resolution. The developed AO method corrects distorted wavefront of Bessel focus at the objective focal plane, demonstrating significant improvements in sensitivity and resolution of structural and functional measurements of synapses in vivo.
NATURE COMMUNICATIONS
(2021)
Article
Radiology, Nuclear Medicine & Medical Imaging
Julien Galley, Reto Sutter, Christoph Germann, Florian Wanivenhaus, Daniel Nanz
Summary: Non-invasive 3D-DESS MR-imaging at 3 and 7 T successfully visualizes the cervical nerve roots within the spinal canal and has the potential to provide precise assessments of their micro-anatomy. Interobserver agreement was substantial to almost perfect, and the ICC for the number of identified rootlets was 0.80.
EUROPEAN RADIOLOGY
(2021)
Article
Multidisciplinary Sciences
David Knez, Benjamin W. Toulson, Anabel Chen, Martin H. Ettenberg, Hai Nguyen, Eric O. Potma, Dmitry A. Fishman
Summary: In this study, a novel spectral imaging technique based on nondegenerate two-photon absorption is proposed for high-speed imaging in the mid-infrared range, allowing simultaneous acquisition of morphological and chemical information.
Article
Biochemical Research Methods
Jelena Platisa, Xin Ye, Allison M. Ahrens, Chang Liu, Ichun Anderson Chen, Ian G. Davison, Lei Tian, Vincent A. Pieribone, Jerry L. Chen
Summary: Monitoring spiking activity in large neuronal populations is crucial for understanding neural circuit function. Voltage imaging provides a new approach for this, but it faces challenges such as reduced fluorescence detection and limited imaging duration. This study developed improved voltage indicators, a high-speed two-photon microscope, and denoising software, enabling simultaneous high-speed deep-tissue imaging of more than 100 labeled neurons over 1 hour. This scalable approach offers a way to image voltage activity across increasing neuronal populations.
Article
Multidisciplinary Sciences
Yonghyeon Jo, Ye-Ryoung Lee, Jin Hee Hong, Dong-Young Kim, Junhwan Kwon, Myunghwan Choi, Moonseok Kim, Wonshik Choi
Summary: DReAM is a label-free technique for deep-tissue imaging that selectively attenuates multiple scattering, providing high-contrast images of neural fibers.
Article
Neurosciences
Wei Qin, Qi Gan, Lei Yang, Yongchao Wang, Weizhi Qi, Bowen Ke, Lei Xi
Summary: The study utilized photoacoustic microscopy to investigate the structural and functional changes of cerebral vasculature in rhesus monkeys, revealing both vasodilatation and vasoconstriction under hypoxic conditions. The results suggest that photoacoustic microscopy is a promising method for studying neurovascular coupling and cerebral vascular diseases.
Article
Biochemistry & Molecular Biology
Jin-Fan Zhang, Bian Liu, Ingie Hong, Albert Mo, Richard H. Roth, Brian Tenner, Wei Lin, Jason Z. Zhang, Rosana S. Molina, Mikhail Drobizhev, Thomas E. Hughes, Lin Tian, Richard L. Huganir, Sohum Mehta, Jin Zhang
Summary: A new ultrasensitive protein kinase A activity reporter has been developed for tracking kinase activity dynamics across various experimental contexts. Results from in vivo visual cortex imaging in awake mice show highly dynamic neuronal PKA activity rapidly recruited by forced locomotion.
NATURE CHEMICAL BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Briana Martinez, Gergey Alzaem Mousa, Kiera Fleck, Tara MacCulloch, Chris W. Diehnelt, Nicholas Stephanopoulos, Sarah E. Stabenfeldt
Summary: The heterogeneous pathophysiology of traumatic brain injury poses challenges for diagnostics and therapeutics. This study used a unique discovery pipeline to identify novel targeting motifs that recognize specific temporal phases of TBI pathology. These findings provide a foundation for the development of targeted TBI therapeutics and diagnostics.
Article
Optics
Congping Chen, Zhongya Qin, Sicong He, Shaojun Liu, Shun-Fat Lau, Wanjie Wu, Dan Zhu, Nancy Y. Ip, Jianan Y. Qu
Summary: The challenge of imaging the brain at high spatial resolution has been addressed with the development of two-photon microscopy integrated with techniques like thinned-skull or optical clearing skull. However, the optical heterogeneity of the skull limits imaging depth to the superficial layer. Recent optimizations in adaptive optics and wavefront sensing algorithms have enabled subcellular resolution imaging of neurons and investigation of microglia-plaque interaction in living brains of mice with Alzheimer's disease.
PHOTONICS RESEARCH
(2021)
Article
Biology
Anuththara Rupasinghe, Nikolas Francis, Ji Liu, Zac Bowen, Patrick O. Kanold, Behtash Babadi
Summary: In this work, a new method is proposed to directly estimate signal and noise correlations from two-photon fluorescence observations without the need for intermediate spike deconvolution. The method provides theoretical guarantees and demonstrates its utility through applications to data from the mouse auditory cortex.