Article
Nanoscience & Nanotechnology
Guofang Zhang, Yalin Cong, Feng-Liang Liu, Jiufeng Sun, Jiantian Zhang, Guoli Cao, Lingqiang Zhou, Wenjie Yang, Qingle Song, Fangjun Wang, Ke Liu, Jing Qu, Jing Wang, Min He, Shun Feng, Didar Baimanov, Wei Xu, Rong-Hua Luo, Xin-Yan Long, Shumin Liao, Yunping Fan, Yu-Feng Li, Bai Li, Ximing Shao, Guocheng Wang, Lijing Fang, Huaiyu Wang, Xue-Feng Yu, Yan-Zhong Chang, Yuliang Zhao, Liang Li, Peng Yu, Yong-Tang Zheng, Diana Boraschi, Hongchang Li, Chunying Chen, Liming Wang, Yang Li
Summary: A biocompatible and antiviral two-dimensional nanomaterial has been reported, which can firmly adsorb the virus and inhibit viral infection through conformational changes induced by interaction with the spike protein. This study proposes CIPS as a promising nanodrug for future anti-SARS-CoV-2 therapy, and also as a decontamination agent and surface-coating material to reduce SARS-CoV-2 infectivity.
NATURE NANOTECHNOLOGY
(2022)
Article
Multidisciplinary Sciences
Cun Li, Jingjing Huang, Yifei Yu, Zhixin Wan, Man Chun Chiu, Xiaojuan Liu, Shuxin Zhang, Jian-Piao Cai, Hin Chu, Gang Li, Jasper Fuk-Woo Chan, Kelvin Kai-Wang To, Zifeng Yang, Shibo Jiang, Kwok-yung Yuen, Hans Clevers, Jie Zhou
Summary: The high transmissibility of SARS-CoV-2 Omicron subvariants is attributed to immune escape. BA.5 showed significantly increased replicative capacity and infectivity in human respiratory epithelial cells. BA.5 has a higher entry efficiency and fusogenic activity, promoting viral spread through syncytium formation in the airway epithelium, while its replicative capacity in alveolar cells is attenuated, which may explain its milder clinical manifestation.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Biochemistry & Molecular Biology
Henkie Isahwan Ahmad Mulyadi Lai, Shih-Jie Chou, Yueh Chien, Ping-Hsing Tsai, Chian-Shiu Chien, Chih-Chien Hsu, Ying-Chun Jheng, Mong-Lien Wang, Shih-Hwa Chiou, Yu-Bai Chou, De-Kuang Hwang, Tai-Chi Lin, Shih-Jen Chen, Yi-Ping Yang
Summary: This study identified the expression of ACE2 and TMPRSS2 in human induced pluripotent stem cell (hiPSC)-derived retinal organoids, and demonstrated the potential infection of SARS-CoV-2 pseudovirus in retinal organoids and monolayer cultures. These findings highlight the potential of iPSC-derived retinal organoids as models for ACE2 receptor-based SARS-CoV-2 infection.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Virology
Man Chun Chiu, Shuxin Zhang, Cun Li, Xiaojuan Liu, Yifei Yu, Jingjing Huang, Zhixin Wan, Xiaoxin Zhu, Jie Zhou
Summary: Researchers have successfully established a three-dimensional organoid model of the respiratory epithelium, which accurately mimics the morphology and function of the human airway epithelium. This model allows for sustained replication of SARS-CoV-2 and accurately recapitulates the infectivity and replicative fitness of different viral variants. The model has significant importance in studying respiratory biology and diseases.
Article
Engineering, Biomedical
Han Kyung Kim, Hyeryeon Kim, Myoung Kyu Lee, Woo Hee Choi, Yejin Jang, Jin Soo Shin, Jun-Yeol Park, Dong Hyuck Bae, Seong-In Hyun, Kang Hyun Kim, Hyun Wook Han, Byungho Lim, Gildon Choi, Meehyein Kim, Young Chang Lim, Jongman Yoo
Summary: This study successfully established tonsil epithelial cell-derived organoids and demonstrated their feasibility as an ex vivo model for SARS-CoV-2 infection. The organoids recapitulated key characteristics of tonsil epithelium and expressed molecules essential for viral entry. Additionally, gene expression changes were observed in the organoids after SARS-CoV-2 infection, and the antiviral drug remdesivir showed efficacy in suppressing viral replication.
Article
Gastroenterology & Hepatology
Jana Krueger, Rudiger Gross, Carina Conzelmann, Janis A. Mueller, Lennart Koepke, Konstantin M. J. Sparrer, Tatjana Weil, Desiree Schuetz, Thomas Seufferlein, Thomas F. E. Barth, Steffen Stenger, Sandra Heller, Jan Muench, Alexander Kleger
Summary: Human intestinal organoids derived from pluripotent stem cells (PSC-HIOs) have been used to study SARS-CoV-2 infection and drug validation, revealing viral spread in the gut and the inhibitory effect of remdesivir. PSC-HIOs are susceptible to infection and can be rescued by drug testing.
CELLULAR AND MOLECULAR GASTROENTEROLOGY AND HEPATOLOGY
(2021)
Article
Cell Biology
Rajasekaran Mahalingam, Prakash Dharmalingam, Abirami Santhanam, Sivareddy Kotla, Gangarao Davuluri, Harry Karmouty-Quintana, Guha Ashrith, Rajarajan A. Thandavarayan
Summary: This study analyzed the expression of SARS-CoV-2 virus in human organoid cells using single-cell RNA sequencing data, finding abundant expression of ACE2 and TMPRSS2 in most organoids. It was discovered that low-density lipoprotein receptor expression is enriched in intestinal, lung, and retinal organoid cells. The study demonstrates that organoids can serve as an experimental platform for investigating the disease mechanism of this novel virus and for drug development.
JOURNAL OF CELLULAR PHYSIOLOGY
(2021)
Article
Microbiology
Romal Stewart, Kexin Yan, Sevannah A. Ellis, Cameron R. Bishop, Troy Dumenil, Bing Tang, Wilson Nguyen, Thibaut Larcher, Rhys Parry, Julian De Jun Sng, Alexander A. Khromykh, Robert K. P. Sullivan, Mary Lor, Frederic A. Meunier, Daniel J. Rawle, Andreas Suhrbier
Summary: Research shows that the BA.5 and XBB sub-lineages have increased pathogenicity compared to the BA.1 sub-lineage in mice and human cortical brain organoids, suggesting an evolving neurotropic potential.
FRONTIERS IN MICROBIOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Dong Chen, Xi Su, Haibo Chen, Siyan Chen, Yongsheng Zhao, Wei Wei
Summary: The COVID-19 pandemic caused by SARS-CoV-2 is a major public health problem. Understanding the molecular mechanisms of the virus is crucial for developing effective therapies and vaccines. Organoid models derived from human stem cells have become valuable tools for studying viral biology and disease progression, as well as identifying potential therapeutic agents. This article summarizes the different types of organoids used in SARS-CoV-2 studies and highlights key findings and their implications for drug development.
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
(2022)
Article
Cell & Tissue Engineering
Sayaka Deguchi, Angel Serrano-Aroca, Murtaza M. Tambuwala, Bruce D. Uhal, Adam M. Brufsky, Kazuo Takayama
Summary: Experimental cell models using human ES/iPS cell-derived somatic cells and organoids play a crucial role in understanding the pathophysiology of COVID-19 and developing therapeutic agents, by replicating SARS-CoV-2 infection and damage in various organs to evaluate antiviral efficacy and safety of potential treatments.
STEM CELLS TRANSLATIONAL MEDICINE
(2021)
Article
Chemistry, Multidisciplinary
Jie Ma, Jia Liu, Dunqin Gao, Xiao Li, Qiyu Zhang, Luye Lv, Yujie Wang, Jun Li, Yunping Zhu, Zhihong Wu, Hengrui Hu, Yufeng Li, Longda Ma, Qian Liu, Zhihong Hu, Shuyang Zhang, Yiwu Zhou, Manli Wang, Ling Leng
Summary: This study reports skin and hair loss in COVID-19 patients, establishes a proteome map of COVID-19 skin, and investigates the susceptibility and pathological mechanisms of skin cells infected with SARS-CoV-2. The findings suggest that SARS-CoV-2 can infect hair follicles and nerve cells, leading to impaired development of follicles and epidermis, and even neuron death.
Article
Microbiology
Man Chun Chiu, Cun Li, Xiaojuan Liu, Wenjun Song, Zhixin Wan, Yifei Yu, Jingjing Huang, Ding Xiao, Hin Chu, Jian-Piao Cai, Kelvin Kai-Wang To, Kwok Yung Yuen, Jie Zhou
Summary: This article reports on an organoid culture system of the nasal epithelium, which is essential for understanding cell biology and virus-host interaction in the human upper respiratory tract. The system allows for efficient reconstruction and stable expansion of the human nasal epithelium, providing a convenient and robust tool for studying SARS-CoV-2 infection and assessing the infectivity of emerging variants.
Review
Cell & Tissue Engineering
Moshe J. Willner, Yang Xiao, Hye Sung Kim, Xuejing Chen, Bin Xu, Kam W. Leong
Summary: The COVID-19 pandemic has worsened the opioid crisis, with factors such as social distancing regulations, medical and financial instability, and mental health issues negatively affecting those with OUD; neurological sequelae in COVID-19 patients suggest compromised neuroimmunity, hypoxia, and respiratory depression, similar to effects of opioid exposure; iPSC-derived brain organoid models may be a useful tool to study the physiological connection between COVID-19 and OUD due to limited in vivo options for research.
JOURNAL OF TISSUE ENGINEERING
(2021)
Review
Cell Biology
Li Peng, Li Gao, Xinya Wu, Yuxin Fan, Meixiao Liu, Jingjing Chen, Jieqin Song, Jing Kong, Yan Dong, Bingxue Li, Aihua Liu, Fukai Bao
Summary: This study summarizes the various lung organoids, their identification biomarkers, and applications. It also outlines the seven coronaviruses capable of infecting humans. Additionally, it provides a detailed summary of existing studies on SARS-CoV-2 using lung organoids, including pathogenesis, drug development, and precision treatment. This review highlights the important value of lung organoids in studying SARS-CoV-2 infection and brings hope for alleviating COVID-19-associated lung infections.
Article
Clinical Neurology
Marco Massimo, Carlotta Barelli, Catalina Moreno, Chiara Collesi, Rebecca K. Holloway, Berta Crespo, Lorena Zentilin, Anna Williams, Veronique E. Miron, Mauro Giacca, Katherine R. Long
Summary: The presence of SARS-CoV-2 in first and second trimester foetal brain tissue is associated with cortical haemorrhages, reduced blood vessel integrity, and increased immune cell infiltration into the foetal brain.
Article
Immunology
Katja Obieglo, Xiaogang Feng, Vishnu Priya Bollampalli, Isabel Dellacasa-Lindberg, Cajsa Classon, Markus Oesterblad, Helena Helmby, James P. Hewitson, Rick M. Maizels, Antonio Gigliotti Rothfuchs, Susanne Nylen
JOURNAL OF IMMUNOLOGY
(2016)
Article
Multidisciplinary Sciences
Khaled N. Alsayegh, Steven D. Sheridan, Shilpa Lyer, Raj Raghavendra Rao
Article
Microbiology
Xiaogang Feng, Cajsa Classon, Graciela Teran, Yunlong Yang, Lei Li, Sherwin Chan, Ulf Ribacke, Antonio Gigliotti Rothfuchs, Jonathan M. Coquet, Susanne Nylen
Article
Multidisciplinary Sciences
Aikaterini Nasi, Vishnu Priya Bollampalli, Meng Sun, Yang Chen, Sylvie Amu, Susanne Nylen, Liv Eidsmo, Antonio Gigliotti Rothfuchs, Bence Rethi
SCIENTIFIC REPORTS
(2017)
Article
Immunology
Nuno Rufino de Sousa, Niklas Sandstrom, Lei Shen, Kathleen Hakansson, Rafaella Vezozzo, Klas Udekwu, Julio Croda, Antonio Gigliotti Rothfuchs
Article
Public, Environmental & Occupational Health
Nuno Rufino de Sousa, Lei Shen, David Silcott, Charles J. Call, Antonio Gigliotti Rothfuchs
ANNALS OF WORK EXPOSURES AND HEALTH
(2020)
Article
Multidisciplinary Sciences
Ioanna Smyrlaki, Martin Ekman, Antonio Lentini, Nuno Rufino de Sousa, Natali Papanicolaou, Martin Vondracek, Johan Aarum, Hamzah Safari, Shaman Muradrasoli, Antonio Gigliotti Rothfuchs, Jan Albert, Bjoern Hoegberg, Bjoern Reinius
NATURE COMMUNICATIONS
(2020)
Article
Immunology
Juliana Bernardi Aggio, Veronika Krmeska, Brian J. Ferguson, Pryscilla Fanini Wowk, Antonio Gigliotti Rothfuchs
Summary: Despite hindering skin DC migration, VACV can still access the draining lymph nodes to prime CD4(+) T cells. UV-inactivated or modified VACV can promote DC migration to the dLN, suggesting that replication-competent VACV is required to interfere with skin DC mobilization.
JOURNAL OF IMMUNOLOGY
(2021)
Article
Immunology
Cajsa H. Classon, Muzhen Li, Ada Lerma Clavero, Junjie Ma, Xiaogang Feng, Christopher A. Tibbitt, Julian M. Stark, Rebeca Cardoso, Emma Ringqvist, Louis Boon, Eduardo J. Villablanca, Antonio Gigliotti Rothfuchs, Liv Eidsmo, Jonathan M. Coquet, Susanne Nylen
Summary: Infection by strictly intestinal helminths can increase the numbers of CD4(+) T cells in the skin of mice, leading to a skewed T(H)2 cell composition and long-term effects on skin immune responses. This indicates a novel gut-to-skin cell trafficking process and worm-mediated immunosuppression.
MUCOSAL IMMUNOLOGY
(2022)
Article
Construction & Building Technology
Nuno Rufino de Sousa, Laura Steponaviciute, Lucille Margerie, Karolina Nissen, Midori Kjellin, Bjorn Reinius, Erik Salaneck, Klas Udekwu, Antonio Gigliotti Rothfuchs
Summary: This study detected aerosol transmission of SARS-CoV-2 in rooms occupied by COVID-19 patients in a hospital in Sweden and found infectious SARS-CoV-2 particles in the air. This suggests the need for revising existing infection control frameworks to include airborne transmission.
Article
Immunology
Veronika Krmeska, Juliana Bernardi Aggio, Susanne Nylen, Pryscilla Fanini Wowk, Antonio Gigliotti Rothfuchs
Summary: This study found that Mycobacterium bovis Bacille Calmette-Guerin (BCG) vaccination induces the migration of dendritic cells (DCs) from the skin to the draining lymph nodes (dLNs) through the production of cyclooxygenase (COX)-derived PGE(2). Live BCG bacilli are necessary for this migration process. It was also found that IL-1 is not required for the early production of COX-2 or PGE(2) in BCG-infected skin, suggesting an IL-1-independent role for PGE(2) in DC migration. Furthermore, EP2/EP4, but not IL-1R, on DCs were shown to be important for the migration process.
JOURNAL OF IMMUNOLOGY
(2022)
Letter
Construction & Building Technology
Nuno Rufino de Sousa, Laura Steponaviciute, Lucille Margerie, Karolina Nissen, Midori Kjellin, Bjorn Reinius, Erik Salaneck, Klas I. Udekwu, Antonio Gigliotti Rothfuchs