The detoxification of cellular sulfite in table grape under SO2 exposure: Quantitative evidence of sulfur absorption and assimilation patterns

Sulfur dioxide (SO2) and its derivatives are known to be hazardous but their common application in food, especially the grape industry, is conditionally allowed. Potential hazards to consumers and the environment could occur upon the control-lost SO2 during grape logistics and storage. Researchers have usually focused on the anti-pathogen role of SO2 whereas limited efforts were conducted on the sulfur (S) absorption, assimilation patterns, and sulfite detoxification. In this study, short-term, room-temperature, and SO2-stored grapes were investigated, whose S flux of various forms was quantified through an estimation model. Accordingly, the additional accumulated S (0.50-0.86%) in pulps from atmospheric SO2 was considered mainly through rachis transport compared to across skin surfaces and the usage arrangement of the absorbed S was included. The first quantitative evidence of induced S assimilation under SO2 was also provided, which challenged the previous knowledge. In addition, sulfite oxidase and reductase (SiO and SiR) played major roles in sulfite detoxification, being effectively stimulated at multiple levels. The induced S metabolism associated with enhanced reactive oxygen species (ROS) scavenging capacity and alleviated senescence contributed to quality maintenance. Overall, these findings provide novel insights and are valuable supports for developing SO2-controlling strategies to avoid potential hazards.

Automated summary

This study reveals that grapes affected by sulfur dioxide primarily accumulate sulfur from the atmosphere through rachis transport, challenging previous knowledge. In sulfite detoxification, sulfite oxidase and reductase play major roles, effectively stimulated at multiple levels.